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1.

001-es BibID:BIBFORM086424
Első szerző:Farkas Etelka (vegyész)
Cím:Dihydroxamate based siderophore model, piperazine-1,4-bis-(N-methyl-acetohydroxamic acid (PIPDMAHA), as a chelating agent of molybdenum(VI) / Etelka Farkas, Hajnalka Csóka, Sofia Gama, Amélia M. Santos
Dátum:2002
ISSN:0039-9140 1873-3573
Megjegyzések:Equilibrium studies based on pH?potentiometric and spectrophotometric measurements as well as some theoretical simulations are reported for the complexes of Mo(VI) with a dihydroxamate type siderophore analogue, the piperazine-1,4-bis-(N-methyl-acetohydroxamic acid) (PIPDMAHA). It has been found that the complexation process starts below pH 2 and that PIPDMAHA forms more stable O,O-hydroxamate bis-chelated complexes with Mo(VI) than any of the formerly studied dihydroxamic acids. The experimental data were fitted with two complexation models based either on dinuclear or on mononuclear species. However, ESI-MS showed that the dimmer is much more abundant than the monomer. This feature was further suggested by theoretical simulation studies, which indicated the dimeric species is more stable than the monomeric one.
Tárgyszavak:Természettudományok Kémiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Siderophore analogues
Molybdenum
Hydroxamic acid
Chelating agent
Megjelenés:Talanta. - 57 : 5 (2002), p. 935-943. -
További szerzők:Csóka Hajnalka Gama, Sofia Santos, Amélia M.
Pályázati támogatás:T23612
OTKA
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DOI
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2.

001-es BibID:BIBFORM071904
Első szerző:Kecskeméti Ádám (vegyész)
Cím:Particle-based immobilized enzymatic reactors in microfluidic chips / Kecskemeti Adam, Gaspar Attila
Dátum:2018
ISSN:0039-9140
Megjegyzések:The research and applications of immobilized enzyme reactors (IMERs) have become more and more widespread due to the numerous advantages like reusability, easy handling, prolonged lifetime, easy separation from pro- ducts and substrate specificity. The miniaturized form of these reactors (microchip IMERs) received outstanding attention due to their special features and advantages over the traditional, larger analytical systems. Large specific surface is essential for the efficient operation of the microreactors, thus these devices include one of the several types of porous solid supports, but in this work only the particle based microchip IMERs are reviewed. A very large variety of micro- or nanoparticles (beads) have been used in the microchip IMERs, however, in- corporating these particles into microchips is still a challenge, because the common procedures used for the preparation of chromatographic columns are not well applicable at the microscopic level. Many detection systems were applied with microchip IMERs using on-chip or off-chip arrangement. The combination of microchip IMERs with mass spectrometry is particularly popular, because in these systems high- throughput analysis can be achieved by which the proteomic studies can be largely accelerated. In most chip IMER-MS systems, the chips are used for sample pretreatment including analyte (protein) digestion, pre- concentration of analyte, removal of matrix materials. Additional applications of the IMERs - like the rapid protein digestion with proteolytic enzymes, the transformation of analytes to a more easily or more sensitively measurable form (detection signal amplification) and the design of microarrays/biosensors to analyze antigens based on specific interactions in immunoanalytical studies ? are also reviewed.
Tárgyszavak:Természettudományok Kémiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
Microfluidic
Enzyme reactor
Particle
Enzyme immobilization
Protein digestion
Megjelenés:Talanta. - 180 (2018), p. 211-228. -
További szerzők:Gáspár Attila (1970-) (vegyész, kémikus)
Pályázati támogatás:OTKA-111932
OTKA
ÚNKP-17-3
Egyéb
GINOP-2.3.3-15-2016-00004
GINOP
GINOP-2.3.2-15-2016-00008
GINOP
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3.

001-es BibID:BIBFORM068560
Első szerző:Kecskeméti Ádám (vegyész)
Cím:Preparation and characterization of a packed bead immobilized trypsin reactor integrated into a PDMS microfluidic chip for rapid protein digestion / Ádám Kecskeméti, Attila Gáspár
Dátum:2017
ISSN:0039-9140
Megjegyzések:This paper demonstrates the design, efficiency and applicability of a simple, inexpensive and high samplethroughput microchip immobilized enzymatic reactor (IMER) for rapid protein digestion. The IMER containsconventional silica particles with covalently immobilized trypsin packed inside of a poly(dimethylsiloxane)(PDMS) microchip channel (10 mm?1 mm?35 ?m). The microchip consists of 9 different channels, enabling 9simultaneous protein digestions. Trypsin was covalently immobilized using carbodiimide activation, the idealtrypsin/silica particle ratio (i.e. measured mass ratio before the immobilization reaction) was determined. Theamount of immobilized trypsin was 10?15 ?g trypsin for 1 mg silica particle. Migration times of CZE peptidemaps showed good repeatability and reproducibility (RSD%=0.02?0.31%). The IMER maintained its activity for2 months, in this period it was used effectively for rapid proteolysis. Four proteins (myoglobin, lysozyme,hemoglobin and albumin) in a wide size range (15?70 kDa) were digested to demonstrate the applicability ofthe reactor. Their CZE peptide maps were compared to peptide maps obtained from standard in-solutiondigestion of the four proteins. The number of peptide peaks correlated well with the theoretically expectedpeptide number in both cases, the peak patterns of the electropherograms were similar, however, digestion withthe microchip IMER requires only < 10 s, while in-solution digestion takes 16 h. LC-MS/MS peptide mappingwas also carried out, the four proteins were identified with satisfying sequence coverages (29?50%), trypsinautolysis peptides were not detected. The protein content of human serum was digested with the IMER and within-solution digestion.
Tárgyszavak:Természettudományok Kémiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
Trypsin immobilization
Protein digestion
Peptide mapping
Microchip reactor
Megjelenés:Talanta. - 166 (2017), p. 275-283. -
További szerzők:Gáspár Attila (1970-) (vegyész, kémikus)
Pályázati támogatás:OTKA-111932
OTKA
NTP-NFTÖ-16-0038
Egyéb
GINOP-2.3.2-15-2016-00008
GINOP
GINOP-2.3.3-15-2016-00004
GINOP
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4.

001-es BibID:BIBFORM016471
Első szerző:Kocúrová, Lívia
Cím:A novel approach in dispersive liquid-liquid microextraction based on the use of an auxiliary solvent for adjustment of density UV-VIS spectrophotometric and graphite furnace atomic absorption spectrometric determination of gold based on ion pair formation / Lívia Kocúrová, Ioseph S. Balogh, Jana Škrlíková, József Posta, Vasil Andruch
Dátum:2010
ISSN:0039-9140
Megjegyzések:This paper presents a novel approach to dispersive liquid-liquid microextraction (DLLME), based on the use of an auxiliary solvent for the adjustment of density. The procedure utilises a solvent system consisting of a dispersive solvent, an extraction solvent and an auxiliary solvent, which allows for the use of solvents having a density lower than that of water as an extraction solvent while preserving simple phase separation by centrifugation. The suggested approach could be an alternative to procedures described in the literature in recent months and which have been devoted to solving the same problem. The efficiency of the suggested approach is demonstrated through the determination of gold based on the formation of the ion pair [Au(CN)(2)](-) anion with Astra Phloxine (R) reagent and its extraction using the DLLME procedure with subsequent UV-VIS spectrophotometric and graphite furnace atomic absorption spectrometric detection. The optimum conditions were found to be: pH 3; 0.8 mmol L-1 K-4[Fe(CN)(6)]; 0.12 mmol L-1 R; dispersive solvent, methanol; extraction solvent, toluene; auxiliary solvent, tetrachloromethane. The calibration plots were linear in the ranges 0.39-4.7 mg L-1 and 0.5-39.4 mu g L-1 for UV-VIS and GFAAS detection, respectively; thus enables the application of the developed method in two ranges differing from one from another by three orders of magnitude. The presented approach can be applied to the development of DLLME procedures for the determination of other compounds extractable by organic solvents with a density lower than that of water. (C) 2010 Elsevier B.V. All rights reserved.
Tárgyszavak:Természettudományok Kémiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
Dispersive liquid-liquid microextraction
Auxiliary solvent
Ion pair
Gold
UV-VIS spectrophotometry
Graphite furnace atomic absorption
spectrometry
mass-spectrometry
water samples
Megjelenés:Talanta (Oxford). - 82 : 5 (2010), p. 1958-1964. -
További szerzők:Balogh Ioseph S. Škrlíková, Jana Posta József (1948-) (vegyész, analitikus) Andruch, Vasil
Internet cím:DOI
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5.

001-es BibID:KLTEbibl000539
Első szerző:Tóth Imre (vegyész)
Cím:Extraction of gallium(III) and aluminium(III) with O,O-dialkyldithiophosphoric acids / Imre Tóth, Ernő Brücher, Zoltán Szabó
Dátum:1990
ISSN:0039-9140 1873-3573
Megjegyzések:The extraction of Ga3+ and Al3+ with the liquid cation-exchangers di-n-butyldithiophosphoric acid (DBTPA) and di-(2-ethylhexyl)dithiophosphoric acid (DETPA) in kerosene, in the presence and absence of alcohols and tri-n-butyl phosphate (TBP) has been studied. Both Ga3+ and Al3+ can be extracted in the form of a neutral complex, MA3, but the distribution coefficient of Ga3+ is the higher by about two orders of magnitude, which can be the basis of the solvent extraction separation of gallium and aluminium. The differences can be explained by the interaction between the sulphur donor atoms of the extractants and the d10 electronic shell of Ga3+ as well as by the lower steric hindrance of ligands co-ordinated to Ga3+.
Tárgyszavak:Természettudományok Kémiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Megjelenés:Talanta. - 37 : 12 (1990), p. 1175-1178. -
További szerzők:Brücher Ernő (1935-) (vegyész) Szabó Zoltán (vegyész)
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
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