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001-es BibID:	BIBFORM039951
035-os BibID:	PMID:11514955
Első szerző:	Balázs Margit (sejtbiológus, molekuláris genetikus)
Cím:	Chromosomal imbalances in primary and metastatic melanomas revealed by comparative genomic hybridization / Margit Balázs, Zsuzsa Ádám, Andrea Treszl, Ágnes Bégány, János Hunyadi, Róza Ádány
Dátum:	2001
ISSN:	0196-4763
Megjegyzések:	Characteristic genetic changes underlying the metastatic progression of malignant melanoma is incompletely understood. The goal of our study was to explore specific chromosomal alterations associated with the aggressive behavior of this neoplasm. Comparative genomic hybridization was performed to screen and compare genomic imbalances present in primary and metastatic melanomas. Sixteen primary and 12 metastatic specimens were analyzed. We found that the pattern of chromosomal aberrations is similar in the two subgroups; however, alterations present only in primary and/or metastatic tumors were also discovered. The mean number of genetic changes was 6.3 (range 1-14) in primary and 7.8 (range 1-16) in metastatic lesions. Frequent losses involved 9p and 10q, whereas gains most often occurred at 1q, 6p, 7q, and 8q. Distinct, high-level amplifications were mapped to 1p12-p21 and 1p22-p31 in both tumor types. Amplification of 4q12-q13.1, 7q21.3-qter and 8q23-qter were detected only in primary tumors. The 20q13-qter amplicon was present in a metastatic tumor. The number of genetic alterations were significantly higher in primary tumors which developed metastases within one year after the surgery compared to tumors without metastasis during this time period. Fluorescence in situ hybridization with centromeric and locus-specific probes was applied to validate CGH results on a subset of tumors. Comparison of FISH and CGH data gave good correlation. The aggressive behavior of melanoma is associated with accumulation of multiple genetic alterations. Chromosome regions, which differ in the primary and metastatic lesions, may represent potential targets to identify metastases-related chromosomal alterations.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	Cytometry. - 46 : 4 (2001), p. 222-232. -
További szerzők:	Ádám Zsuzsa Bégány Ágnes (1954-2011) (bőrgyógyász, kozmetológus, klinikai onkológus) Hunyadi János (1943-) (bőrgyógyász, kozmetológus, allergológus) Treszl Andrea (1974-) (molekuláris biológus) Ádány Róza (1952-) (megelőző orvostan és népegészségtan szakorvos)
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001-es BibID:	BIBFORM015977
Első szerző:	Balázs Margit (sejtbiológus, molekuláris genetikus)
Cím:	Interphase cytogenetic analysis shows frequent hemizygous and homozygous deletion of p16/MTS1/CDKN2A tumorsuppressor gene in sporadic primary melanomas / Balazs M., Rakosy Z., Treszl A., Begany A., Adany R.
Dátum:	2004
ISSN:	1552-4922
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idézhető absztrakt
Megjelenés:	22nd Congress of the International-Society-for-Analytical-Cytology (22)(2004)(Franciaország). - Cytometry Part A. - 59A : 1 (2004), p. 69. -
További szerzők:	Rákosy Zsuzsa (1978-) (sejtbiológus, molekuláris biológus, genetikus) Bégány Ágnes (1954-2011) (bőrgyógyász, kozmetológus, klinikai onkológus) Ádány Róza (1952-) (megelőző orvostan és népegészségtan szakorvos) Treszl Andrea (1974-) (molekuláris biológus)
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM005605
Első szerző:	Kiss Flóra (bőrgyógyász)
Cím:	A coagulation factor becomes useful in the study of acute leukemias : studies with blood coagulation factor XIII / Kiss F., Simon Á., Csáthy L., Hevessy Z., Katona É., Kiss C., Kappelmayer J.
Dátum:	2008
Megjegyzések:	The intracellular form of the coagulation factor XIII has previously been identified by immunomorphological techniques using polyclonal antibodies. In these studies, only the A subunit (FXIII-A) was detectable in megakaryocytes/platelets and in monocytes/ macrophages. We developed several novel monoclonal antibody clones directed to both subunits (FXIII-A and FXIII-B) and investigated their appearance in normal and leukemic cells. By using 3- and 4-color flow cytometry FXIII expression was investigated in normal peripheral blood and bone marrow samples and in acute myeloblastic (AML) and lymphoblastic (ALL) leukemia cases. Samples were studied by Western blotting and confocal laser scanning microscopy. With a previously published ELISA assay applying two monoclonal antibodies directed to different epitopes in FXIII-A, we were able to measure the intracytoplasmic content of FXIII-A in normal cells and leukemic blasts. FXIII-A was detectable in normal peripheral blood monocytes and in large quantities in platelets, but both cell types were negative for FXIII-B. There was no surface staining for FXIII-A, it only appeared intracellularly. In samples derived from patients with AML M4 and M5, FXIII-A sensitively identified blast cells. Although normal lymphocytes do not express FXIII-A, 40% of ALL cases showed significant FXIII-A expression as determined by flow cytometry. FXIII-A positivity of lymphoblasts was verified by Western blotting, ELISA, and confocal laser scanning microscopy cytometry. These data provide evidence that FXIII-A is a sufficiently sensitive marker in differentiating myeloblasts and monoblasts and is suitable for identifying leukemia-associated phenotypes in ALL.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban factor XIII flow cytometry acute leukemia phenotype
Megjelenés:	Cytometry. Part A. - 73A : 3 (2008), p. 194-201. -
További szerzők:	Simon Ágnes (1969-) (laboratóriumi szakorvos) Csáthy László (1979-) (laboratóriumi szakorvos) Hevessy Zsuzsanna (1966-) (laboratóriumi szakorvos) Katona Éva (1961-) (klinikai biokémikus) Kiss Csongor (1956-) (hematológus, onkológus) Kappelmayer János (1960-) (laboratóriumi szakorvos)
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001-es BibID:	BIBFORM041645
035-os BibID:	PMID:15221868
Első szerző:	Treszl Andrea (molekuláris biológus)
Cím:	Extra copies of c-myc are more pronounced in nodular melanomas than in superficial spreading melanomas as revealed by fluorescence in situ hybridisation / Andrea Treszl, Róza Ádány, Zsuzsa Rákosy, László Kardos, Ágnes Bégány, Katalin Gilde, Margit Balázs
Dátum:	2004
ISSN:	0196-4763
Megjegyzések:	Amplification of c-myc is a common genetic alteration and associated with a poor prognosis in a variety of cancers. Extra copies of the gene have been found in large numbers of melanoma metastases, but only few primary tumours have been studied. We investigated the c-myc copy number alterations in two different subtypes of primary melanomas with different biological behaviours. METHODS: Fluorescence in situ hybridisation was performed using c-myc and centromeric 8 (C8) probes on 68 lesions (28 nodular melanomas [NMs], 26 superficial spreading melanomas [SSMs], and 14 metastases). To assess the ploidy pattern, copy number distribution of seven different chromosomes was also investigated. RESULTS: All tumours showed aneuploid populations for at least three chromosomes. Whereas 61% of the NMs exhibited extra c-myc copies, only 27% of SSMs showed increased gene dosage. The c-myc/C8 ratio exceeding 1.5 was significantly higher in NMs (P = 0.01). High level amplification was seen only in NMs. An elevated c-myc/C8 ratio was higher than 1.5 in only four metastases. CONCLUSION: Our data show that c-myc copy number alterations differ in the two melanoma subtypes and are associated with the advanced stage of the disease. The less frequent amplification of the c-myc gene in metastatic lesions indicates that it may play an important role in the development of an invasive potential rather than in the metastatic process.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban nodular melanoma superficial spreading melanoma c-myc fluorescence in situ hybridisation egyetemen (Magyarországon) készült közlemény
Megjelenés:	Cytometry. Part B, Clinical Cytometry. - 60B : 1 (2004), p. 37-46. -
További szerzők:	Ádány Róza (1952-) (megelőző orvostan és népegészségtan szakorvos) Rákosy Zsuzsa (1978-) (sejtbiológus, molekuláris biológus, genetikus) Kardos László (1970-) (megelőző orvostan és népegészségtan szakorvos) Bégány Ágnes (1954-2011) (bőrgyógyász, kozmetológus, klinikai onkológus) Gilde Katalin Balázs Margit (1952-) (sejtbiológus, molekuláris genetikus)
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