Találati lista | Tudóstér

001-es BibID:	BIBFORM066281
Első szerző:	Balogh Enikő (molekuláris biológus)
Cím:	Impaired Immunosuppressive Effect of Bronchoalveolar Mesenchymal Stem Cells in Hypersensitivity Pneumonitis : preliminary findings / Eniko Balogh, Bela Nagy Jr., Agnes Gyetvai, Zsolt Bene, Zoltan Hendrik, Viktoria Jeney, Peter Nagy, Agnes Papp, Jozsef Balla, Gyorgy Balla, Janos Kappelmayer, Bela Nagy
Dátum:	2018
ISSN:	1552-4949
Megjegyzések:	Bronchoalveolar mesenchymal stem cells (MSCs) play an important role in the maintenance of lung integrity. Therapeutic application of bone marrow-derived MSCs reduced chronic bronchial inflammation in idiopathic pulmonary fibrosis, and improved the ratio of survivors in sepsis with pneumonia. This study investigated the effect of MSCs from bronchoalveolar lavage fluid (BALF) of hypersensitivity pneumonitis (HP) on T-cell function under in vitro conditions.METHODS:Bronchoalveolar MSCs were obtained via bronchoscopy with BAL from children with severe subacute HP. As control, BALF MSCs were assessed from children without any inflammatory lung disease. Isolated MSCs were characterized via immunophenotyping by flow cytometry and confocal laser scanning microscopy. HP-derived and healthy separated peripheral blood mononuclear cells (PBMCs) were stimulated by 5 ?g/mL phytohemagglutinin in the presence of HP-derived or control MSCs in 5-day cultures. Proliferation and activation of T-cells were characterized by the mean fluorescence intensity (MFI) of 5,6-carboxyfluorescein-diacetat succinimidyl ester (CFSE) and CD25, CD69 as well as HLA-DR surface positivities, respectively.RESULTS:HP-derived MSCs showed significantly lower level of CD73, CD90, and CD105 expression compared to control MSCs in both flow cytometric and confocal microscopic experiments. MSCs from HP did not reduce T-cell proliferation based on CFSE MFI values, while the level of CD25 expression on both control and HP-derived CD4+ and CD8+ T-cells was significantly reduced by normal MSCs, while HP-derived MSCs did not have any significant effect. The level of other activation markers was not markedly modulated by MSCs.CONCLUSIONS:BALF MSCs from HP are unable to downregulate the proliferation and activation of T-cells that may support the development of recurrent intrapulmonary inflammation in HP. ? 2016 Clinical Cytometry Society.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban T-lymphocyte proliferation and activation bronchoalveolar lavage hypersensitivity pneumonitis mesenchymal stem cells
Megjelenés:	Cytometry Part B-Clinical Cytometry. - 94 : 2 (2018), p. 363-368. -
További szerzők:	Nagy Béla Jr. (1980-) (labordiagnosztikai szakorvos) Gyetvai Ágnes Bene Zsolt (1981-) (orvos) Hendrik Zoltán (1986-) (orvos) Jeney Viktória (1971-) (vegyész, kémia tanár) Nagy Péter (1971-) (biofizikus) Papp Ágnes (1967-) (gyermekgyógyász, pulmonológus) Balla József (1959-) (belgyógyász, nephrológus) Balla György (1953-) (csecsemő és gyermekgyógyász, neonatológus) Kappelmayer János (1960-) (laboratóriumi szakorvos) Nagy Béla (1949-) (csecsemő- és gyermekgyógyász, gyermek-tüdőgyógyász)
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
Borító:
Saját polcon:

001-es BibID:	BIBFORM062383
Első szerző:	Kerényi Adrienne (laboratóriumi szakorvos)
Cím:	Evaluation of flow cytometric HIT assays in relation to an IgG-specific immunoassay and clinical outcome / Adrienne Kerényi, Ildikó Beke Debreceni, Zsolt Oláh, Péter Ilonczai, Zsuzsanna Bereczky, Béla Nagy Jr., László Muszbek, János Kappelmayer
Dátum:	2017
ISSN:	1552-4949
Megjegyzések:	BACKGROUND:Heparin-induced thrombocytopenia (HIT) is a severe side effect of heparin treatment caused by platelet activating IgG antibodies generated against the platelet factor 4 (PF4)-heparin complex. Thrombocytopenia and thrombosis are the leading clinical symptoms of HIT.METHODS:The clinical pretest probability of HIT was evaluated by the 4T score system. Laboratory testing of HIT was performed by immunological detection of antibodies against PF4-heparin complex (EIA) and two functional assays. Heparin-dependent activation of donor platelets by patient plasma was detected by flow cytometry. Increased binding of Annexin-V to platelets and elevated number of platelet-derived microparticles (PMP) were the indicators of platelet activation.RESULTS:EIA for IgG isotype HIT antibodies was performed in 405 suspected HIT patients. Based on negative EIA results HIT was excluded in 365 (90%) of cases. In 40 patients with positive EIA test result functional tests were performed. Platelet activating antibodies were detected in 17 cases by Annexin V binding. PMP count analysis provided nearly identical results. The probability of a positive flow cytometric assay result was higher in patients with elevated antibody titer. 71% of patients with positive EIA and functional assay had thrombosis.CONCLUSIONS:EIA is an important first line laboratory test in the diagnosis of HIT, however, HIT must be confirmed by a functional test. Annexin V binding and PMP assays using flow cytometry are functional HIT tests convenient in a clinical diagnostic laboratory. The positive results of functional assays may predict the onset of thrombosis. This article is protected by copyright. All rights reserved.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban heparin-induced thrombocytopenia thrombosis 4T score flow cytometry platelet microparticles
Megjelenés:	Cytometry Part B-Clinical Cytometry. - 92 : 5 (2017), p. 389-397. -
További szerzők:	Bekéné Debreceni Ildikó (1970-) (biológus) Oláh Zsolt (1974-) (belgyógyász) Ilonczai Péter (1977-) (orvos, belgyógyász, haematológus szakorvos) Bereczky Zsuzsanna (1974-) (orvosi laboratóriumi diagnosztika szakorvos) Nagy Béla Jr. (1980-) (labordiagnosztikai szakorvos) Muszbek László (1942-) (haematológus, kutató orvos) Kappelmayer János (1960-) (laboratóriumi szakorvos)
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
Borító:
Saját polcon:

001-es BibID:	BIBFORM006003
Első szerző:	Szabó Gábor (biofizikus)
Cím:	Flow cytometric analysis of the uptake of Hoechst 33342 dye by human lymphocytes / Gábor Szabó, Attila Kiss, Sándor Damjanovich
Dátum:	1981
Megjegyzések:	Human peripheral blood lymphocytes have been shown to resist staining with the DNA binding fluorochrome Hoechst 33342 by the cellular membrane. The rate of uptake of the dye is strongly temperature-dependent with minimal uptake rate below 16 degrees C. The activation energy of dye transport was found to be 135 kJ/mol above 20 degrees C and about 20 kJ/mol below 16 degrees C. Metabolic inhibitors accelerated, instead of inhibiting, the transport of the dye. Dead cells have been shown to stain promptly in contrast with the gradually staining viable cells. The uptake process in the vital staining conditions is suggested to involve a carrier mediated mechanism. Application of Hoechst 33342 as a fluorescent indicator of viability is proposed.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban analysis Azides Benzimidazoles Biological Transport blood Cell Survival Dna Dyes Flow Cytometry Fluorescent Dyes Human Kinetics Lymphocytes metabolism pharmacology Sodium Sodium Azide Staining and Labeling Support,Non-U.S.Gov't Temperature
Megjelenés:	Cytometry. - 2 : 1 (1981), p. 20-23. -
További szerzők:	Kiss Attila (1942-) (belgyógyász, haematológus) Damjanovich Sándor (1936-2017) (biofizikus)
Internet cím:	elektronikus változat DOI
Borító:
Saját polcon:

001-es BibID:	BIBFORM077428
Első szerző:	Szerémy Péter
Cím:	Determination of reference values of MDR-ABC transporter activities in CD3+ lymphocytes of healthy volunteers using a flow cytometry based method / Szerémy Péter, Tauberné Jakab Katalin, Baráth Sándor, Apjok András, Filkor Kata, Holló Zsolt, Márki-Zay János, Kappelmayer János, Sipka Sándor, Krajcsi Péter, Toldi Gergely
Dátum:	2019
ISSN:	1552-4949
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk
Megjelenés:	Cytometry Part B-Clinical Cytometry. - 96 : 6 (2019), p. 469-474. -
További szerzők:	Tauberné Jakab Katalin (Szeged) Baráth Sándor (1977-) (biológus) Apjok András Filkor Kata Holló Zsolt Marki-Zay János Kappelmayer János (1960-) (laboratóriumi szakorvos) Sipka Sándor ifj. (1980-) (orvos) Krajcsi Péter Toldi Gergely
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
Borító:
Saját polcon: