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001-es BibID:	BIBFORM105401
035-os BibID:	(cikkazonosító)1003709 (WoS)000868652700001 (Scopus)85139183146
Első szerző:	Kocsis Beatrix (molekuláris biológus)
Cím:	Functional analysis of the bZIP-type transcription factors AtfA and AtfB in Aspergillus nidulans / Beatrix Kocsis, Mi-Kyung Lee, Jae-Hyuk Yu, Tibor Nagy, Lajos Daróczi, Gyula Batta, István Pócsi, Éva Leiter
Dátum:	2022
ISSN:	1664-302X
Megjegyzések:	Transcription factors (TFs) with the basic leucin zipper domain are key elements of the stress response pathways in filamentous fungi. In this study, we functionally characterized the two bZIP type TFs AtfA and AtfB by deletion (Delta) and overexpression (OE) of their encoding genes in all combination: Delta atfA, Delta atfB, Delta atfA Delta atfB, Delta atfAatfBOE, Delta atfBatfAOE, atfAOE, atfBOE and atfAOEatfBOE in Aspergillus nidulans. Based on our previous studies, Delta atfA increased the sensitivity of the fungus to oxidative stress mediated by menadione sodium bisulfite (MSB) and tert-butylhydroperoxide (tBOOH), while Delta atfB was not sensitive to any oxidative stress generating agents, namely MSB, tBOOH and diamide at all. Contrarily, the Delta atfB mutant was sensitive to NaCl, but tolerant to sorbitol. Overexpression of atfB was able to compensate the MSB sensitivity of the Delta atfA mutant. Heavy metal stress elicited by CdCl2 reduced diameter of the atfBOE and atfAOEatfBOE mutant colonies to about 50% of control colony, while the cell wall stress generating agent CongoRed increased the tolerance of the Delta atfA mutant. When we tested the heat stress sensitivity of the asexual spores (conidiospores) of the mutants, we found that conidiospores of Delta atfAatfBOE and Delta atfBatfAOE showed nearly 100% tolerance to heat stress. Asexual development was negatively affected by Delta atfA, while atfAOE and atfAOE coupled with Delta atfB increased the number of conidiospores of the fungus approximately 150% compared to the control. Overexpression of atfB led to a 25% reduction in the number of conidiospores, but increased levels of abaA mRNA and size of conidiospores. Sexual fruiting body (cleistothecium) formation was diminished in the Delta atfA and the Delta atfA Delta atfB mutants, while relatively elevated in the Delta atfB and the Delta atfBatfAOE mutants. Production of the mycotoxin sterigmatocystin (ST) was decreased to undetectable levels in the Delta atfA mutant, yet ST production was restored in the Delta atfA Delta atfB mutant, suggesting that Delta atfB can suppress ST production defect caused by Delta atfA. Levels of ST were also significantly decreased in the Delta atfAatfBOE, Delta atfBatfAOE and atfAOEatfBOE mutants.
Tárgyszavak:	Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk aspergillus nidulans environmental stress conidiospore cleistothecium sterigmatocystin
Megjelenés:	Frontiers in Microbiology. - 13 (2022), p. 1-9. -
További szerzők:	Lee Mi-Kyung Yu Jae-Hyuk Nagy Tibor (1988-) (vegyész) Daróczi Lajos (1965-) (fizikus) Batta Gyula (1979-) (biológus) Pócsi István (1961-) (vegyész) Leiter Éva (1976-) (biológus)
Pályázati támogatás:	NKFIH Egyéb
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM085057
035-os BibID:	(cikkazonosító)957 (WOS)000539331800001 (Scopus)85085883554
Első szerző:	Nagy Fruzsina (molekuláris biológus)
Cím:	In vitro and in vivo effect of exogenous farnesol exposure against Candida auris / Fruzsina Nagy, Eszter Vitális, Ágnes Jakab, Andrew M. Borman, Lajos Forgács, Zoltán Tóth, László Majoros, Renátó Kovács
Dátum:	2020
ISSN:	1664-302X
Megjegyzések:	The spreading of multidrug-resistant Candida auris is considered as an emerging global health threat. The number of effective therapeutic regimens is strongly limited; therefore, development of novel strategies is needed. Farnesol is a quorum-sensing molecule with a potential antifungal and/or adjuvant effect; it may be a promising candidate in alternative treatment against Candida species including C. auris. To examine the effect of farnesol on C. auris, we performed experiments focusing on growth, biofilm production ability, production of enzymes related to oxidative stress, triazole susceptibility and virulence. Concentrations ranging from 100 to 300 uM farnesol caused a significant growth inhibition against C. auris planktonic cells for 24 hours (p<0.01-0.05). Farnesol treatment showed a concentration dependent inhibition in terms of biofilm forming ability of C. auris; however, it did not inhibit significantly the biofilm development at 24 hours. Nevertheless, the metabolic activity of adhered farnesol pre-exposed cells (75 uM) was significantly diminished at 24 hours depending on farnesol treatment during biofilm formation (p<0.001-0.05). Moreover, 300 uM farnesol exerted a marked decrease in metabolic activity against one-day-old biofilms between 2 and 24 hours (p<0.001). Farnesol increased the production of reactive species remarkably, as revealed by 2',7'-dichlorofluorescein (DCF) assay (3.96?0.89 [nmol DCF (OD640)-1] and 23.54?4.51 [nmol DCF (OD640)-1] for untreated cells and farnesol exposed cells, respectively; p<0.001). This was in line with increased superoxide dismutase level (85.69?5.42 [munit (mg protein)-1] and 170.11?17.37 [munit (mg protein)-1] for untreated cells and farnesol exposed cells, respectively; p<0.001), but the catalase level remained statistically comparable between treated and untreated cells (p>0.05). Concerning virulence-related enzymes, exposure to 75 uM farnesol did not influence phospholipase or aspartic proteinase activity (p>0.05). The interaction between fluconazole, intraconazole, voriconazole, posaconazole, isavuconazole and farnesol showed clear synergism (FICI ranges from 0.038 to 0.375) against one-day-old biofilms. Regarding in vivo experiments, daily 75 uM farnesol treatment decreased the fungal burden in an immuncompromised murine model of disseminated candidiasis, especially in case of inocula pre-exposed to farnesol (p<0.01). In summary, farnesol shows a promising therapeutic or adjuvant potential in traditional or alternative therapies such as catheter lock therapy.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk
Megjelenés:	Frontiers in Microbiology. - 11 (2020), p. 1-12. -
További szerzők:	Vitális Eszter (1977-) (orvos) Jakab Ágnes (1987-) (biológus) Borman, Andrew M. Forgács Lajos Tóth Zoltán (1990-) (molekuláris biológus) Majoros László (1966-) (szakorvos, klinikai mikrobiológus) Kovács Renátó László (1987-) (molekuláris biológus)
Pályázati támogatás:	EFOP-3.6.3-VEKOP-16-2017-00009 EFOP ÚNKP-19-3 Egyéb
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM100299
035-os BibID:	(cikkazonosító)785411 (WOS)000749862300001 (Scopus)85123770402
Első szerző:	Nagy József Bálint (molekuláris biológus)
Cím:	Comparison of Extended-Spectrum Beta-Lactamase-Producing Escherichia coli Isolates From Rooks (Corvus frugilegus) and Contemporary Human-Derived Strains: A One Health Perspective / Nagy Bálint József, Balázs Bence, Benmazouz Isma, Gyüre Péter, Kövér László, Kaszab Eszter, Bali Krisztina, Lovas-Kiss Ádám, Damjanova Ivelina, Majoros László, Tóth Ákos, Bányai Krisztián, Kardos Gábor
Dátum:	2022
ISSN:	1664-302X
Tárgyszavak:	Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk
Megjelenés:	Frontiers in Microbiology. - 12 (2022), p. 1-9. -
További szerzők:	Balázs Bence (1991-) (PhD hallgató) Benmazouz, Isma (1992-) (veterinarian) Gyüre Péter (1974-) (agrármérnök) Kövér László (1985-) (városökológia, városi vadgazdálkodás) Kaszab Eszter (1989-) (biológus) Bali Krisztina Lovas-Kiss Ádám (1991-) (biológus, botanikus) Damjanova, Ivelina Majoros László (1966-) (szakorvos, klinikai mikrobiológus) Tóth Ákos Bányai Krisztián Kardos Gábor
Pályázati támogatás:	ÚNKP-19-3-I Egyéb
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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