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001-es BibID:	BIBFORM090372
Első szerző:	Kusza Szilvia (agrármérnök)
Cím:	Preliminary report on β-casein gene Met183QVal183 polymorphism in Romanian indigenous Zackel sheep breeds / Kusza Szilvia, Daniela Elena Ilie, Maria Sauer, Ioan-Walter Sauer, Dinu Gavojdian
Dátum:	2017
ISSN:	0001-527X 1734-154X
Megjegyzések:	Genetic polymorphisms of the milk protein genes are important because of their effects on quantitative traits and technological properties of milk manufacturing. In the present study we identified the polymorphism of the beta-casein gene in two local sheep breeds (Racka n=98 and Turcana n=111) in Romania. The most studied variants at the ovine beta-casein (CSN2) locus are: A and G variants. Genomic DNA was extracted from hair follicles and beta-casein genotypes were determined by the rapid TaqMan (Applied Biosystems, USA) genotyping assay. Homozygote genotypes GG were not detected in any of the studied breeds. In both Racka and Turcana breeds, A variant had a much higher frequency, 0.98% and 0.97%, respectively. In the current study the fast DNA tests for genotyping ovine CSN2 was successfully optimized, however, further samples and correlations of genomic results with milk characteristics and production data are needed for the development of future selection schemes of the Romanian indigenous sheep breeds, with the ultimate purpose to produce low level allergenic sheep milk and derived dairy products.
Tárgyszavak:	Agrártudományok Állatorvosi tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk gene polymorphism ovine milk Racka Turcana
Megjelenés:	Acta Biochimica Polonica. - 64 : 2 (2017), p. 339-341. -
További szerzők:	Ilie, Daniela Elena Sauer, Maria Sauer, Ioan-Walter Gavojdian, Dinu
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM069248
Első szerző:	Kusza Szilvia (agrármérnök)
Cím:	Preliminary result of a genetic polymorphism of [beta]-lactoglobulin gene and the phylogenetic study of ten balkan and central european indigenous sheep breeds / Szilvia Kusza, Nikolett Sziszkosz, Krisztina Nagy, Amela Masala, Sándor Kukovics, Jávor András
Dátum:	2015
ISSN:	0001-527X
Megjegyzések:	Genetic polymorphism at the [beta]-lactoglobulin ([beta]-LG) loci in indigenous sheep breeds (Tsigai, Racka, Pramenka) was determined. Altogether 904 sheep were genotyped for the presence of the A, B and C alleles of [beta]-lactoglobulin by PCR-RFLP. The AB genotype was the most common and the [beta]-lactoglobulin A was the most frequent in the Cokanski Tsigai (54%), while the B allele was the most common in the Rusty and the Zomborski Tsigai (59%, 60%). The C allele was found only in one individual from Serbian Cokanski flock. These results differ from those that refer to other native sheep breeds. In the Cokanski Tsigai, deviation from the Hardy-Weinberg equilibrium was detected. Genetic relationship based on [beta]-lactoglobulin polymorphism was the closest between the Rusty and the Cokanski Tsigai among the studied populations and between sheep and goat among the other ruminants. Part of the promoter region (254 bp) of [beta]-LG in studied sheep breeds were sequenced in order to identify polymorphisms, analyze haplotypes, and phylogenetic relationship among them. Sequencing analysis and alignment of the obtained sequences showed one haplotype. Analysis of more samples and longer parts of the promoter region of [beta]-LG are needed to reconstruct a phylogenetic tree.
Tárgyszavak:	Agrártudományok Állattenyésztési tudományok idegen nyelvű folyóiratközlemény külföldi lapban Tsigai group Zackel sheep [beta]-LG allele frequencies, genetic relationship sequencing
Megjelenés:	Acta Biochimica Polonica 62 : 1 (2015), p. 109-112. -
További szerzők:	Sziszkosz Nikolett (1988-) (molekuláris biológus) Nagy Krisztina (agrár) Masala, Amela Kukovics Sándor (1950-) (agrár) Jávor András (1952-) (agrármérnök)
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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