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001-es BibID:	BIBFORM072554
035-os BibID:	(cikkazonosító)e0193935 (WOS)000426902900050 (Scopus)85043481861
Első szerző:	Bagi Zoltán (természetvédelmi mérnök, állatgenetika)
Cím:	MtDNA genetic diversity and structure of Eurasian Collared Dove (Streptopelia decaocto) / Zoltán Bagi, Evangelos Antonis Dimopoulos, Dimitrios Loukovitis, Cyril Eraud, Szilvia Kusza
Dátum:	2018
ISSN:	1932-6203
Megjegyzések:	The Eurasian Collared Dove (Streptopelia decaocto) is one of the most successful biological invaders among terrestrial vertebrates. However, little information is available on the genetic diversity of the species. A total of 134 Eurasian Collared Doves from Europe, Asia and the Caribbean (n=20) were studied by sequencing a 658-bp length of mitochondrial DNA (mtDNA) cytochrome oxidase I (COI). Fifty-two different haplotypes and relatively high haplotype and nucleotide diversities (Hd?SD= 0.843?0.037 and ??SD= 0.026?0.013) were detected. Haplotype Ht1 was particularly dominant: it included 44.03% of the studied individuals, and contained sequences from 75% of the studied countries. Various analyses (FST, AMOVA, STRUCTURE) distinguished 2 groups on the genetic level, designated 'A' and 'B'. Two groups were also separated in the median-joining network and the maximum likelihood tree. The results of the neutrality tests were negative (Fu FS= -25.914; Tajima D= -2.606) and significantly different from zero (P?0.001) for group A, whereas both values for group B were positive (Fu FS= 1.811; Tajima D= 0.674) and not significant (P>0.05). Statistically significant positive autocorrelation was revealed among individuals located up to 2000 km apart (r=0.124; P=0.001). The present results provide the first information on the genetic diversity and structure of the Eurasian Collared Dove, and can thereby serve as a factual and comparative basis for similar studies in the future.
Tárgyszavak:	Agrártudományok Állattenyésztési tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk demographic expansion mitochondrial DNA COI non-invasive sampling Columbidae haplotypes
Megjelenés:	Plos One. - 13 : 3 (2018), p. e0193935. -
További szerzők:	Dimopoulos, Evangelos Antonis Loukovitis, Dimitrios Eraud, Cyril Kusza Szilvia (1979-) (agrármérnök)
Pályázati támogatás:	EFOP-3.6.3-VEKOP-16-2017-00008 EFOP
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat DOI
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001-es BibID:	BIBFORM088166
035-os BibID:	(cikkazonosító)e013873
Első szerző:	Ilie, Daniela Elena
Cím:	Microsatellite and Mitochondrial DNA Study of Native Eastern European Cattle Populations: The Case of the Romanian Grey / Ilie Daniela Elena, Cean Ada, Cziszter Ludovic Toma, Gavojdian Dinu, Iván Alexandra, Kusza Szilvia
Dátum:	2015
ISSN:	1932-6203 1932-6203
Megjegyzések:	The Eastern European Grey cattle are regarded as the direct descendants of the aurochs(Bos taurus primigenius). Nowadays in Romania, less than 100 Grey animals are being reared and included in the national gene reserve. We examined the genetic diversity among Romanian Grey, Brown, Spotted and Black and White cattle breeds, with a particular focuson Romanian Grey through the use of (i) 11 bovine specific microsatellite markers on 83 animals and (ii) 638 bp length of mitochondrial DNA (mtDNA) D-loop region sequence data from a total of 81 animals. Both microsatellite and mtDNA analysis revealed a high level of genetic variation in the studied breeds. In Romanian Grey a total of 100 alleles were found,the mean number of observed alleles per locus was 9.091; the average observed heterozygosity was 0.940; the Wright's fixation index (FIS) was negative (-0.189) and indicates that there is no inbreeding and no selection pressure. MtDNA analysis revealed 52 haplotypes with 67 variable sites among the Romanian cattle breeds without any insertion or deletion.Haplotype diversity was 0.980?0.007 and ranged from 0.883?0.056 (Brown) to 0.990?0.028 (Spotted and Black and White). The highest genetic variability of the mtDNA was recorded in the Grey breed, where 18 haplotypes were identified. The most frequent mtDNAD-loop region belonged to T3 haplogroup (80.247%), which was found across all studied breeds, while T2 haplotypes (16.049%) was only found in Grey, Spotted and Black and White genotypes. The T1 haplotypes (3.704%) were found in the Grey and Spotted. The current results contribute to the general knowledge on genetic diversity found in Eastern European cattle breeds and could prove a valuable tool for the conservation efforts of animal genetic resources (FAnGR).
Tárgyszavak:	Agrártudományok Állattenyésztési tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk DNA Study Native Eastern European Cattle Populations Romanian Grey
Megjelenés:	Plos One. - 10 : 9 (2015), p. 1-18. -
További szerzők:	Cean, Ada Cziszter, Ludovic Toma Gavojdian, Dinu Iván Alexandra Kusza Szilvia (1979-) (agrármérnök)
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM079295
035-os BibID:	(cikkazonosító)e0197051 (WOS)000431757400057 (Scopus)85046772044
Első szerző:	Ilie, Daniela Elena
Cím:	Genetic characterization of indigenous goat breeds in Romania and Hungary with a special focus on genetic resistance to mastitis and gastrointestinal parasitism based on 40 SNPs / Daniela Elena Ilie, Kusza Szilvia, Maria Sauer, Dinu Gavojdian
Dátum:	2018
ISSN:	1932-6203
Megjegyzések:	Goat breeding has become an important sector in Eastern Europe, with Romania and Hungary being among the major producer countries. Given the limited number of research done up-to-date concerning genetic studies of indigenous goat breeds reared in Romania and Hungary, the current preliminary study aimed to analyze the variability of genes related to mastitis and gastrointestinal parasitism by using Kompetitive Allele Specific PCR (KASP?). We studied 52 single nucleotide polymorphisms (SNPs) belonging to 19 genes in indigenous breeds from both countries, namely Banat's White (n = 36), Carpatina (n = 35) from Romania and Hungarian Milking (n = 79) and identified 16 polymorphic SNPs among 10 genes (PTX3, IL6, CLEC4E, IL8, IL1RN, IL15RA, TNFSF13, SOCS3, TNF and TLR3) in 150 animals. Furthermore, the diversity of the studied breeds was investigated. The PIC values ranged from 0.042 to 0.691. The mean values of observed and expected heterozygosity were 0.235 and 0.246 respectively. The highest observed heterozygosity was obtained for IL15RA g.10343904C>T in Banat's White (0.464), IL15RA g.10354813C>T in Carpatina (0.577) and SOCS3 g.52626440T>G in Hungarian Milking (0.588). Pairwise FST values between the Romanian breeds and Romanian and Hungarian breeds were small (0.009 and 0.015), indicating the close relationship among the studied goat populations. From all the polymorphic SNPs identified, the Hungarian Milking breed showed the highest proportion of polymorphisms (100%), whereas the Carpatina breed had the lowest percentage (87.5%). The highest value of MAF was obtained for SOCS3 g.52626440T>G (0.46), IL15RA g.10343904C>T (0.47), IL15RA g.10344025C>T (0.45), and IL15RA g.10354813C>T (0.42). The 16 polymorphic SNPs identified in a panel of 150 unrelated individuals belonging to three Romanian and Hungarian indigenous goat breeds could be used in future genomic based breeding schemes as markers for genetic resistance to mastitis and gastrointestinal parasitism in goat breeds found in Eastern and Central Europe.
Tárgyszavak:	Agrártudományok Állattenyésztési tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk
Megjelenés:	Plos One. - 13 : 5 (2018), p. 1-14. -
További szerzők:	Kusza Szilvia (1979-) (agrármérnök) Sauer, Maria Gavojdian, Dinu
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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