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001-es BibID:	BIBFORM119550
035-os BibID:	(cikkazonosító)2825 (scopus)85180451810 (wos)001131446900001
Első szerző:	Ghaffarinia, Ameneh
Cím:	Unraveling Transcriptome Profile, Epigenetic Dynamics, and Morphological Changes in Psoriasis-like Keratinocytes : "Insights into Similarity with Psoriatic Lesional Epidermis" / Ghaffarinia Ameneh, Póliska Szilárd, Ayaydin Ferhan, Goblos Aniko, Parvaneh Shahram, Manczinger Máté, Balogh Fanni, Erdei Lilla, Veréb Zoltán, Szabó Kornélia, Bata-Csörgő Zsuzsanna, Kemény Lajos
Dátum:	2023
ISSN:	2073-4409
Megjegyzések:	Abstract: Keratinocytes are one of the primary cells affected by psoriasis inflammation. Our study aimed to delve deeper into their morphology, transcriptome, and epigenome changes in response to psoriasis-like inflammation. We created a novel cytokine mixture to mimic mild and severe psoriasis-like inflammatory conditions in cultured keratinocytes. Upon induction of inflammation, we observed that the keratinocytes exhibited a mesenchymal-like phenotype, further confirmed by increased VIM mRNA expression and results obtained from confocal microscopy. We performed RNA sequencing to achieve a more global view, revealing 858 and 6987 DEGs in mildly and severely inflamed keratinocytes, respectively. Surprisingly, we found that the transcriptome of mildly inflamed keratinocytes more closely mimicked that of the psoriatic epidermis transcriptome than the severely inflamed keratinocytes. Genes involved in the IL-17 pathway were a major contributor to the similarities of the transcriptomes between mildly inflamed KCs and psoriatic epidermis. Mild and severe inflammation led to the gene regulation of epigenetic modifiers such as HATs, HDACs, DNMTs, and TETs. Immunofluorescence staining revealed distinct 5-hmC patterns in inflamed versus control keratinocytes, and consistently low 5-mC intensity in both groups. However, the global DNA methylation assay detected a tendency of decreased 5-mC levels in inflamed keratinocytes versus controls. This study emphasizes how inflammation severity affects the transcriptomic similarity of keratinocytes to psoriatic epidermis and proves dynamic epigenetic regulation and adaptive morphological changes in inflamed keratinocytes.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk psoriasis keratinocyte cytokine inflammation transcriptome
Megjelenés:	Cells. - 12 : 24 (2023), p. 1-24. -
További szerzők:	Póliska Szilárd (1978-) (biológus) Ayaydin, Ferhan Goblos Anikó Parvaneh, Shahram Manczinger Máté Balogh Fanny (1995-) (hallgató) Erdei Lilla Veréb Zoltán (1980-) (immunológus, mikrobiológus, molekuláris biológus) Szabó Kornélia Bata-Csörgő Zsuzsanna Kemény Lajos V. (bőrgyógyász Szeged)
Pályázati támogatás:	TKP2021-NKTA-34 FIKP
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM116204
035-os BibID:	(Scopus)85167596616 (WOS)001046691600001 (cikkazonosító)1966
Első szerző:	Szűcs Diána
Cím:	Effect of Inflammatory Microenvironment on the Regenerative Capacity of Adipose-Derived Mesenchymal Stem Cells / Szűcs Diána, Miklós Vanda, Monostori Tamás, Guba Melinda, Kun-Varga Anikó, Póliska Szilárd, Kis Erika, Bende Balázs, Kemény Lajos, Veréb Zoltán
Dátum:	2023
ISSN:	2073-4409
Megjegyzések:	Adipose-derived mesenchymal stem cells are increasingly being used in regenerative medicine as cell therapy targets, including in the treatment of burns and ulcers. The regenerative potential of AD-MSCs and some of their immunological properties are known from in vitro studies; however, in clinical applications, cells are used in non-ideal conditions and can behave differently in inflammatory environments, affecting the efficacy and outcome of therapy. Our aim was to investigate and map the pathways that the inflammatory microenvironment can induce in these cells. High-throughput gene expression assays were performed on AD-MSCs activated with LPS and TNF?. Analysis of RNA-Seq data showed that control, LPS-treated and TNF?-treated samples exhibited distinct gene expression patterns. LPS treatment increased the expression of 926 genes and decreased the expression of 770 genes involved in cell division, DNA repair, the cell cycle, and several metabolic processes. TNF? treatment increased the expression of 174 genes and decreased the expression of 383 genes, which are related to cell division, the immune response, cell proliferation, and differentiation. We also map the biological pathways by further investigating the most altered genes using the Gene Ontology and KEGG databases. Secreted cytokines, which are important in the immunological response, were also examined at the protein level, and a functional assay was performed to assess wound healing. Activated AD-MSC increased the secretion of IL-6, IL-8 and CXCL-10, and also the closure of wounds. AD-MSCs presented accelerated wound healing under inflammation conditions, suggesting that we could use this cell in clinical application.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk adipose-derived mesenchymal stem cells inflammation lipopolysaccharide regenerative medicine tumor necrosis factor
Megjelenés:	Cells. - 12 : 15 (2023), p. 1-19. -
További szerzők:	Miklós Vanda Monostori Tamás Guba Melinda Kun-Varga Anikó Póliska Szilárd (1978-) (biológus) Kis Erika (Szeged) Bende Balázs Kemény Lajos V. (bőrgyógyász Szeged) Veréb Zoltán (1980-) (immunológus, mikrobiológus, molekuláris biológus)
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM088858
035-os BibID:	(cikkazonosító)8847038 (WoS)000591736500001 (Scopus)85096040529
Első szerző:	Veréb Zoltán (immunológus, mikrobiológus, molekuláris biológus)
Cím:	Vessel Wall-Derived Mesenchymal Stromal Cells Share Similar Differentiation Potential and Immunomodulatory Properties with Bone Marrow-Derived Stromal Cells / Veréb Zoltán, Mázló Anett, Szabó Attila, Póliska Szilárd, Kiss Attila, Litauszky Krisztina, Koncz Gábor, Boda Zoltán, Rajnavölgyi Éva, Bácsi Attila
Dátum:	2020
ISSN:	1687-966X 1687-9678
Megjegyzések:	This study is aimed at investigating the phenotype, differentiation potential, immunomodulatory properties, and responsivenessof saphenous vein vessel wall-derived mesenchymal stromal cells (SV-MSCs) to various TLR ligands and proinflammatory cytokines, aswell as comparing their features to those of theirbone marrow-derived counterparts (BM-MSCs).Methods. SV-MSCs were isolated byenzymatic digestion of the saphenous vein vessel wall. Phenotype analysis was carried out byflow cytometry and microscopy, whereasadipogenic, chondrogenic, and osteogenic differentiation potentials were tested inin vitroassays. For comparative analysis, theexpression of different stemness, proliferation, and differentiation-related genes was determined by Affymetrix gene array. To comparethe immunomodulatory properties of SV-MSCs and BM-MSCs, mixed lymphocyte reaction was applied. To investigate theirresponses to various activating stimuli, MSCs weretreated with TLR ligands (LPS, PolyI:C) or proinflammatory cytokines (TNF?,IL-1?,IFN?), and the expression of various early innate immune response-related genes was assessed by qPCR, while secretion ofselected cytokines and chemokines was measured by ELISA.Results. The isolated SV-MSCs were able to differentiate into bone, fat,and cartilage cells/directionin vitro. SV-MSCs expressed the most important MSC markers (CD29, CD44, CD73, CD90, and CD105)and shared almost identical phenotypic characteristics with BM-MSCs. Their gene expression pattern and activation pathways wereclose to those of BM-MSCs. SV-MSCs showed better immunosuppressive activity inhibiting phytohemagglutinin-induced Tlymphocyte proliferationin vitrothan BM-MSCs. Cellular responses to treatments mimicking inflammatory conditions werecomparable in the bone marrow- and saphenous vein-derived MSCs. Namely, similar to BM-MSCs, SV-MSCs secreted increasedamount of IL-6 and IL-8 after 12- or 24-hour treatment with LPS, PolyI:C, TNF?,orIL-1?,comparedtountreatedcontrols.Interestingly, a different CXCL-10/IP-10 secretion pattern could be observed under inflammatory conditions in the two types ofMSCs.Conclusion. Based on our results, cells isolated from saphenous vein vessel wall fulfilled the ISCT's (International Society forCellular Therapy) criteria for multipotent mesenchymal stromal cells, and no significant differences in the phenotype, gene expressionpattern, and responsiveness to inflammatory stimuli could be observed between BM-MSCs and SV-MSCs, while the latter cells havemore potent immunosuppressive activityin vitro. Further functional assays have to be performed to reveal whether SV-MSCs couldbe useful for certain regenerative therapeutic applications or tissueengineering purposes.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk
Megjelenés:	Stem Cells International. - 2020 (2020), p. 1-16. -
További szerzők:	Türk-Mázló Anett (1989-) (molekuláris biológus) Szabó Attila (1981-) (molekuláris biológus, immunológus, filozófus) Póliska Szilárd (1978-) (biológus) Kiss Attila (1942-) (belgyógyász, haematológus) Litauszky Krisztina (1967-) (orvos) Koncz Gábor (1970-) (biológus, immunológus) Boda Zoltán (1947-) (belgyógyász, haematologus, klinikai onkológus) Rajnavölgyi Éva (1950-) (immunológus) Bácsi Attila (1967-) (immunológus)
Pályázati támogatás:	GINOP-2.3.2-15-2016-00050 GINOP OTKA-114423 OTKA NKFIH K 125337 Egyéb
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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