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001-es BibID:	BIBFORM064998
Első szerző:	Dózsa Anikó (Ph.D hallgató, orvos)
Cím:	PPAR[gamma] has different expression and signaling pattern in normal and pathologic sebaceous glands / A. Dozsa, B. I. Tóth, T. Bíró, E. Remenyik, B. Dezso, C. C. Zouboulis, L. Nagy
Dátum:	2009
Megjegyzések:	Peroxisome proliferation activated receptor gamma (PPAR?) has been implicated in lipid metabolismand also in infl ammation. PPAR? is expressed in lipid rich human sebocytes and it appears to haveroles in their functions. The details of PPAR?-regulated lipid metabolism in sebocytes are, however,not well understood. Therefore our aim was to characterize the expression patterns of PPAR?, itsheterodimeric partner RXR and target genes in normal and pathologic sebaceous glands in tissuesand in the SZ95 sebocytes . Therefore we applied formaldehyde fi xed parraffi n embedded skinsamples of patients with sebaceous hyperplasia, sebaceous carcinoma for immunohistochemistry.From fresh frozen skin samples, we laser microdissected normal sebaceous glands and isolatedRNA for RT-PCR. We studied function of PPAR? molecule in SZ95 sebocyte culture by addingPPAR? agonist rosiglitazone (RSG), RXRalfa agonist LG268 or arachidonic acid (AA). Viabilityof the cells was measured by MTT test. Nile red staining and quantitative fl uorimetric analysiswere used to detect changes in polar and neutral lipid content levels as a differentiation markerof sebocytes. We found that PPAR? and RXR? proteins are present in normal and hyperplasticsebaceous glands, immortalized SZ95 cell line, but PPAR? is barely expressed in sebaceouscarcinoma. We also demonstrated that mRNA of PPAR? and its lipid-metabolism associatedtarget genes, ADRP and PGAR were present in both models, but only sebaceous glands in situexpressed FABP4. Target genes responded to activation or inhibition of PPAR? and RXR? inSZ95 sebocytes. These data suggest that PPAR? as a transcription factor is likely to play a role innormal and pathological sebaceous gland biology, thus possibly serving as a relevant target forfurther investigations in sebaceous gland-associated dermatoses and a potential target of therapy.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idézhető absztrakt PPAR gamma sebocyte lipid
Megjelenés:	Journal of Investigative Dermatology. - 129 : Suppl. 2 (2009), p. S54. -
További szerzők:	Tóth István Balázs (1978-) (élettanász) Bíró Tamás (1968-) (élettanász) (absztraktok) Remenyik Éva (1956-) (bőrgyógyász) Dezső Balázs (1951-) (pathológus) Zouboulis, Christos C. (1960-) (bőrgyógyász) Nagy László (1966-) (molekuláris sejtbiológus, biokémikus)
Internet cím:	Szerző által megadott URL Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM064997
Első szerző:	Dózsa Anikó (Ph.D hallgató, orvos)
Cím:	Role of a lipid activated transcription factor peroxisome proliferation activated receptor gamma in sebocytes / A. Dozsa, B. I. Tóth, T. Bíró, E. Remenyik, B. Dezso, C. C. Zouboulis, L. Nagy
Dátum:	2007
ISSN:	0022-202X
Megjegyzések:	Sebaceous gland is a holocrine gland with accelerated lipid metabolism. On the otherhand, peroxisome proliferation activated receptor gamma (PPAR?) is a nuclear receptor,functioning as a transcriptional factor. Together with retinoid X receptor alpha (RXR?) it formsa heterodimer and regulates gene expression. Certain lipid ligands activate or inactivate thisreceptor complex. PPAR? has a key role in adipocyte lipid metabolism and differentiation;sebaceous glands were also reported to express PPAR?. Our goal is to assess if PPAR? hasa role in sebocyte function and/or diseases associated with this cell type. Human SZ95sebocytes and skin samples of healthy individuals and patients with dermatoses were usedfor immunhistochemistry to determine the presence of the receptor proteins. In addition,we isolated mRNA from laser microdissected sebaceous glands to detect gene expressionwith RTqPCR. The function of the receptor was studied in SZ95 sebocytes by adding thePPAR? agonist rosiglitazone (RSG), the PPAR? antagonist GW9668, interleukin4 (IL4), theRXR? agonist LG268 or arachidonic acid (AA). Quantitative fluorimetric analyses wereused to detect changes in lipids. We detected PPAR? and RXR? proteins in samples fromheathy individuals, patients with sebaceous hyperplasia, sebaceous carcinoma and inSZ95 sebocytes. The expression of PPARs in SZ95 sebocytes and also expression of targetgenes (ie. ADRP, LXR?) were detectable. By adding RSG, IL4 and AA no change in certainPPAR? target genes was detected, however changes in sebocyte lipid content occurred.GW9668 treatment decreased sebaceous lipids. Our data suggest that PPAR? is likelyto play a role in sebaceous lipid synthesis, thus it is possibly a relevant target for furtherinvestigation in sebaceous gland-associated dermatoses and a potential target of therapy.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idézhető absztrakt PPAR gamma sebocyte lipid
Megjelenés:	Journal of Investigative Dermatology. - 127 : Suppl. 2 (2007), p. S81. -
További szerzők:	Tóth István Balázs (1978-) (élettanász) Bíró Tamás (1968-) (élettanász) (absztraktok) Remenyik Éva (1956-) (bőrgyógyász) Dezső Balázs (1951-) (pathológus) Zouboulis, Christos C. (1960-) (bőrgyógyász) Nagy László (1966-) (molekuláris sejtbiológus, biokémikus)
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM084536
035-os BibID:	(WOS)000574374700012 (Scopus)85083321686
Első szerző:	Retzlerné Medgyesi Barbara (biotechnológus)
Cím:	Rosacea is Characterized by a Profoundly Diminished Skin Barrier / Medgyesi B., Dajnoki Zs., Béke G., Gáspár K., Szabó I. L., Janka E. A., Póliska S., Hendrik Z., Méhes G., Törőcsik D., Bíró T., Kapitány A., Szegedi A.
Dátum:	2020
ISSN:	0022-202X 1523-1747
Megjegyzések:	Rosacea is a common, chronic inflammation of sebaceous gland-rich facial skin characterized by severe skin dryness, elevated pH, transepidermal water loss, and decreased hydration levels. Until now, there has been no thorough molecular analysis of permeability barrier alterations in the skin of rosacea patients. Thus, we aimed to investigate the barrier alterations in papulopustular rosacea (PPR) samples compared to healthy sebaceous gland-rich (SGR) skin, using RNASeq analysis (n=8). Pathway analyses by Cytoscape ClueGo revealed 15 significantly enriched pathways related to skin barrier formation. RT-PCR and immunohistochemistry were used to validate the pathway analyses. The results showed significant alterations in barrier components in PPR samples compared to SGR, including the cornified envelope and intercellular lipid lamellae formation, desmosome and tight junction organizations, barrier alarmins, and antimicrobial peptides. Moreover, the barrier damage in PPR was unexpectedly similar to atopic dermatitis (AD); this similarity was confirmed by immunofluorescent staining. In summary, besides the well-known dysregulation of immunological, vascular, and neurological functions, we demonstrated prominent permeability barrier alterations in PPR at the molecular level, which highlight the importance of barrier repair therapies for rosacea.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk antimicrobial peptides barrier function cornified envelope papulopustular rosacea
Megjelenés:	The Journal of investigative dermatology. - 140 : 10 (2020), p. 1938-1950. -
További szerzők:	Dajnoki Zsolt (1985-) (molekuláris biológus) Béke Gabriella (1987-) (molekuláris biológus) Gáspár Krisztián (1974-) (bőrgyógyász) Szabó Imre Lőrinc (1987-) (általános orvos) Janka Eszter Anna (1989-) (bőrgyógyász, népegészségügyi szakember) Póliska Szilárd (1978-) (biológus) Hendrik Zoltán (1986-) (orvos) Méhes Gábor (1966-) (patológus) Töröcsik Dániel (1979-) (bőrgyógyász) Bíró Tamás (1968-) (élettanász) Kapitány Anikó (1979-) (molekuláris biológus) Szegedi Andrea (1964-) (bőrgyógyász)
Internet cím:	DOI Intézményi repozitóriumban (DEA) tárolt változat
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