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001-es BibID:BIBFORM050943
Első szerző:Szalma József (Pécs)
Cím:Proteomic and scanning electron microscopic analysis of submandibular sialoliths / József Szalma, Katalin Böddi, Edina Lempel, Alexandra Forsayeth Sieroslawska, Zoltán Szabó, Rania Harfouche, Lajos Olasz, Anikó Takátsy, András Guttman
Dátum:2013
ISSN:1432-6981 1436-3771
Megjegyzések:ObjectivesSeveral theories have been proposed regarding the genesis of sialoliths, including the organic core theory, which suggests epithelial or bacterial etiology originating in the central core. Our aim was to use novel methodologies to analyze central areas (the core) of calculi from sialolithiasis patients.Materials and methodsThe structures of the halves of six submandibular salivary stones were analyzed by scanning electron microscopy (SEM). After structural analysis, from the other six halves, samples from the central parts of the core and peripheral parts of the core were digested with trypsin and analyzed by matrix-assisted laser desorption ionization?time of flight mass spectrometry. The peptide mass fingerprints were compared with the results of in silico digestion.ResultsSEM analysis of the sialoliths showed that organic structures (collagen/fibrous-like structures, bacterial fragments) were visible only outside of the core in the concentric layers of external areas, but not in the core area. The mass spectrometry (MS)/MS post-source decay experiments were completed from the four, most intense signals observed in the MS spectrum and human defensin was proven to be present in three of the examined samples, originated from the peripheral region of three cores.ConclusionsAlthough proteomic analysis demonstrated defensin protein in the peripheral region of the core in three sialoliths, SEM failed to prove organic structures in the core.Clinical relevanceNew investigation modalities still cannot prove organic structures in the core, henceforward challenging the organic core theory.
Tárgyszavak:Természettudományok Kémiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
Sialolith
Calculus
Etiology
Submandibular gland
SEM
MALDI-TOF
Defensin
Megjelenés:Clinical Oral Investigations. - 17 : 7 (2013), p. 1709-1717. -
További szerzők:Böddi Katalin Lempel Edina Sieroslawska, Alexandra Forsayeth Szabó Zoltán (Pécs) Harfouche, Rania Olasz Lajos Takátsy Anikó Guttman András (1954-) (vegyészmérnök)
Pályázati támogatás:K-81839
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