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001-es BibID:	BIBFORM117022
035-os BibID:	(cikkazonosító)21001 (Scopus)85177798219
Első szerző:	Adil Ali, Maysaa
Cím:	All-trans retinoic acid and dexamethasone regulate phagocytosis-related gene expression and enhance dead cell uptake in C2C12 myoblast cells / Maysaa Adil Ali, Éva Garabuczi, Nastaran Tarban, Zsolt Sarang
Dátum:	2023
ISSN:	2045-2322
Megjegyzések:	Extensive mechanical stress frequently causes micro-traumas in skeletal muscle, followed by a regeneration period. The effective removal of dead myofibers is a prerequisite for proper regeneration, and several cell types, including professional phagocytes, were reported to be active in this process. Myoblasts express several molecules of the phagocytic machinery, such as BAI1, stabilin-2, and TAM (Tyro3, Axl, Mertk) tyrosine kinase receptors, but these molecules were reported to serve primarily cell fusion and survival, and their role in the phagocytosis was not investigated. Therefore, we aimed to investigate the in vitro phagocytic capacity of the C2C12 mouse myoblast cell line. RNA sequencing data were analyzed to determine the level and changes of phagocytosis-related gene expression during the differentiation process of C2C12 cells. To study the phagocytic capacity of myoblasts and the effect of dexamethasone, all-trans retinoic acid, hemin, and TAM kinase inhibitor treatments on phagocytosis, C2C12 cells were fed dead thymocytes, and their phagocytic capacity was determined by flow cytometry. The effect of dexamethasone and all-trans retinoic acid on phagocytosis-related gene expression was determined by quantitative PCR. Both undifferentiated and differentiated cells engulfed dead cells being the undifferentiated cells more effective. In line with this, we observed that the expression of several phagocytosis-related genes was downregulated during the differentiation process. The phagocytosis could be increased by dexamethasone and all-trans retinoic acid and decreased by hemin and TAM kinase inhibitor treatments. Our results indicate that myoblasts not only express phagocytic machinery genes but are capable of efficient dead cell clearance as well, and this is regulated similarly, as reported in professional phagocytes.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk
Megjelenés:	Scientific Reports. - 13 : 1 (2023), p. 1-8. -
További szerzők:	Garabuczi Éva (1982-) (biokémikus, molekuláris biológus) Tarban, Nastaran (1988-) (molekuláris biológus) Sarang Zsolt (1976-) (mikrobiológus)
Pályázati támogatás:	NKFIH 124244 Egyéb
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM119954
035-os BibID:	(cikkazonosító)7545
Első szerző:	Nagy Nikoletta Andrea (biológus)
Cím:	The updated genome of the Hungarian population of Aedes koreicus / Nikoletta Andrea Nagy, Gábor Endre Tóth, Kornélia Kurucz, Gábor Kemenesi, Levente Laczkó
Dátum:	2024
ISSN:	2045-2322
Megjegyzések:	Vector-borne diseases pose a potential risk to human and animal welfare, and understanding their spread requires genomic resources. The mosquito Aedes koreicus is an emerging vector that has been introduced into Europe more than 15 years ago but only a low quality, fragmented genome was available. In this study, we carried out additional sequencing and assembled and characterized the genome of the species to provide a background for understanding its evolution and biology. The updated genome was 1.1Gbp long and consisted of 6099 contigs with an N50 value of 329,610 bp and a BUSCO score of 84%. We identifed 22,580 genes that could be functionally annotated and paid particular attention to the identifcation of potential insecticide resistance genes. The assessment of the orthology of the genes indicates a high turnover at the terminal branches of the species tree of mosquitoes with complete genomes, which could contribute to the adaptation and evolutionary success of the species. These results could form the basis for numerous downstream analyzes to develop targets for the control of mosquito populations.
Tárgyszavak:	Orvostudományok Egészségtudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk Aedes Invasive mosquito Genome assembly Tird generation sequencing Hybrid assembly Functional annotation
Megjelenés:	Scientific Reports. - 14 : 1 (2024), p.1-12. -
További szerzők:	Tóth Gábor Endre Kurucz Kornélia Kemenesi Gábor Laczkó Levente (1992-) (biológus)
Pályázati támogatás:	PD142602 OTKA FK-138563 Egyéb RRF-2.3.1-21-2022-00010 Egyéb
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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