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001-es BibID:	BIBFORM076545
035-os BibID:	(WoS)000461584100005 (Scopus)85062210557
Első szerző:	Budai Zsófia (biológus, molekuláris biológus)
Cím:	Macrophages engulf apoptotic and primary necrotic thymocytes through similar phosphatidylserine-dependent mechanisms / Budai Zsófia, Ujlaky-Nagy László, Kis Gréta Nikoletta, Antal Miklós, Bankó Csaba, Bacsó Zsolt, Szondy Zsuzsa, Sarang Zsolt
Dátum:	2019
ISSN:	2211-5463
Megjegyzések:	One of the major roles of professional phagocytes is the removal of dead cells in the body. We know less about the clearance of necrotic cells than apoptotic cell phagocytosis, despite the fact that both types of dead cells need to be cleared together, and necrotic cells appear often in pathological settings. In the present study, we examined phagocytosis of heat- or H2O2-killed necrotic and apoptotic thymocytes by mouse bone marrow-derived macrophages (BMDMs) in vitro, and found that the two cell types are engulfed at equal efficiency and compete with each other when added together to BMDMs. Phagocytosis of both apoptotic and necrotic thymocytes was decreased by (i) blocking phosphatidylserine on the surface of dying cells; (ii) inhibition of Mer tyrosine kinase, Tim-4, integrin ?3 receptor signaling, or Rac1 activity; or (iii) using BMDMs deficient for transglutaminase 2. Stimulation of liver X, retinoid X, retinoic acid or glucocorticoid nuclear receptors in BMDMs enhanced not only apoptotic, but also necrotic cell uptake. Electron microscopic analysis of the engulfment process revealed that the morphology of phagosomes and the phagocytic cup formed during the uptake of dying thymocytes is similar for apoptotic and necrotic cells. Our data indicate that apoptotic and necrotic cells are cleared via the same mechanisms, and removal of necrotic cells in vivo can be facilitated by molecules known to enhance the uptake of apoptotic cells.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk macrophages phagocytosis primary necrosis apoptosis phosphatidylserine
Megjelenés:	FEBS Open Bio. - 9 (2019), p. 446-456. -
További szerzők:	Ujlaky-Nagy László (1977-) (biofizikus) Kis Gréta (1979-) (környezetkutató) Antal Miklós (1951-) (orvos, anatómus) Bankó Csaba (1989-) (molekuláris biológus) Bacsó Zsolt (1963-) (biofizikus) Szondy Zsuzsanna (1959-) (molekuláris sejtbiológus, biokémikus) Sarang Zsolt (1976-) (mikrobiológus)
Pályázati támogatás:	EFOP-3.6.3-VEKOP-16-2017-00009 EFOP GINOP-2.3.2-15-2016-00006 GINOP
Internet cím:	DOI Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM071996
035-os BibID:	(WoS)000428997200007 (Scopus)85042164621
Első szerző:	Csősz Éva (biokémikus, molekuláris biológus)
Cím:	Salivary proteome profiling of oral squamous cell carcinoma in a Hungarian population / Éva Csősz, Bernadett Márkus, Zsuzsanna Darula, Katalin F. Medzihradszky, Judit Nemes, Emese Szabó, József Tőzsér, Csongor Kiss, Ildikó Márton
Dátum:	2018
ISSN:	2211-5463
Megjegyzések:	Oral squamous cell carcinoma (OSCC) is the 7th most common malignancy and the 9th most frequent site of cancer death in Europe. Hungary occupies top places of statistics regarding OSCC incidence and mortality. Therefore, there is a high need to improve early detection. Saliva, as a readily available body fluid, became an increasingly important substance for the detection of biomarkers. Different research groups identified salivary biomarkers specific for different countries. In this study saliva samples of Hungarian OSCC patients were studiedin order to discover disease- and perhaps region-specific biomarkers. LC/MS/MS analysis on linear ion trap-Orbitrap mass spectrometer was used for qualitative and quantitative salivary protein profiling. More than 500 proteins were identified from saliva by shotgun proteomics. The up- and downregulated proteins in the saliva of patients with OSCC highlighted the importance of protein-protein interaction networks involving the immune system and proteolysis in disease development. Two potential biomarkers from our shotgun analysis and a third candidate reported earlier by a Taiwanese group were further examined by ELISA on a larger reference set of samples. Resistin reported as a biomarker in Taiwan but not validated in our study highlights the necessity of application of standardized analysis methods in different ethnic or geographical populations in order to identify biomarkers with sufficient specificity and sensitivity.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk OSCC biomarker proteomic analysis ELISA
Megjelenés:	FEBS Open Bio. - 8 : 4 (2018), p. 556-569. -
További szerzők:	Márkus Bernadett (1989-) (molekuláris biológus) Darula Zsuzsanna Medzihradszky-Fölkl Katalin Nemes Judit (1968-) (fogszakorvos) Szabó Emese (1992-) (biotechnológus) Tőzsér József (1959-) (molekuláris biológus, biokémikus, vegyész) Kiss Csongor (1956-) (hematológus, onkológus) Márton Ildikó (1954-) (fogszakorvos)
Pályázati támogatás:	OTKA-105034 OTKA TÁMOP-4.2.2.D-15/1/KONV-2015-0016 TÁMOP GINOP-2.3.2-15-2016-00020 Egyéb GINOP-2.3.2-15-2016-00001 Egyéb
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat DOI Szerző által megadott URL
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