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1.

001-es BibID:BIBFORM046090
Első szerző:Bacsó Zsolt (biofizikus)
Cím:Raft and cytoskeleton associations of an ABC transporter : P-glycoprotein / Zsolt Bacso, Henrietta Nagy, Katalin Goda, László Bene, Ferenc Fenyvesi, János Matkó, Gábor Szabó
Dátum:2004
ISSN:0196-4763
Megjegyzések:A novel flow cytometric assay has been described in an accompanying report (Gombos et al., METHODS: The kinetics of the decrease in immunofluorescence intensity was analyzed after the addition of the raft-preserving Triton X-100 or Nonidet P-40, both of which disrupt the entire membrane. Mild treatments by both detergents leave cells attached to only those proteins that are anchored to the cytoskeleton by rafts or independent of rafts. Agents that affect microfilaments and modulate membrane levels of cholesterol by cyclodextrin were used to distinguish between the raft-mediated and non-raft-related associations of the Pgp. Confocal microscopy and flow cytometric fluorescence energy transfer measurements were used to confirm colocalization of Pgp with raft constituents. RESULTS: The assay was proved to be sensitive enough to resolve differences between the resistance of UIC2-labeled cell-surface Pgps to Triton X-100 versus Nonidet P-40. Approximately 34% of the UIC2 Fab-labeled Pgp molecules were associated with the cytoskeleton through detergent-resistant, cholesterol-sensitive microdomains or directly, whereas approximately 15% were found to be directly linked to the cytoskeleton. Accordingly, confocal microscopy showed that Pgps colocalize with raft markers, mainly in microvilli. Fluorescence resonance energy transfer efficiency data indicating molecular proximity between Pgp and the raft markers CD44, CD59, and G(M1)-gangliosides also suggested that a significant fraction of Pgps resides in raft microdomains. Raft association of Pgp appears to be of functional significance because its modulation markedly affected drug pumping. CONCLUSIONS: By using the flow cytometric detergent resistance assay in kinetic mode, we were able to assess the extent of raft association and actin cytoskeleton anchorage of Pgp expressed at physiologically relevant levels. We demonstrated that a significant fraction of Pgp is raft associated on LS-174-T human colon carcinoma cells and that this localization may influence its transporter function. The kinetic flow cytometric detergent resistance assay presented in this report is considered to be generally applicable for the analysis of molecular interactions of membrane proteins expressed at low levelsújratöltve - BIBFORM004828
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Cytometry. - 61 : 2 (2004), p. 105-116. -
További szerzők:Nagy Henrietta Goda Katalin (1969-) (biofizikus) Bene László (1963-) (biofizikus) Fenyvesi Ferenc (1977-) (gyógyszerész, gyógyszertechnológus) Matkó János (1952-) (biológus) Szabó Gábor (1953-) (biofizikus)
Pályázati támogatás:T032563
OTKA
034393
OTKA
T046945
OTKA
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DOI
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2.

001-es BibID:BIBFORM015977
Első szerző:Balázs Margit (sejtbiológus, molekuláris genetikus)
Cím:Interphase cytogenetic analysis shows frequent hemizygous and homozygous deletion of p16/MTS1/CDKN2A tumorsuppressor gene in sporadic primary melanomas / Balazs M., Rakosy Z., Treszl A., Begany A., Adany R.
Dátum:2004
ISSN:1552-4922
Tárgyszavak:Orvostudományok Elméleti orvostudományok idézhető absztrakt
Megjelenés:22nd Congress of the International-Society-for-Analytical-Cytology (22)(2004)(Franciaország). - Cytometry Part A. - 59A : 1 (2004), p. 69. -
További szerzők:Rákosy Zsuzsa (1978-) (sejtbiológus, molekuláris biológus, genetikus) Bégány Ágnes (1954-2011) (bőrgyógyász, kozmetológus, klinikai onkológus) Ádány Róza (1952-) (megelőző orvostan és népegészségtan szakorvos) Treszl Andrea (1974-) (molekuláris biológus)
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
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3.

001-es BibID:BIBFORM015661
Első szerző:Juhász Attila (fül-orr-gégész)
Cím:Chromosomal imbalances in laryngeal and hypopharyngeal cancers detected by comparative genomic hybridization / Juhász A., Balázs M., Sziklay I., Rákosy Zs., Treszl A., Répássy G., Ádány R.
Dátum:2005
ISSN:1552-4922
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Cytometry. Part A. - 67 : 2 (2005), p. 151-160. -
További szerzők:Balázs Margit (1952-) (sejtbiológus, molekuláris genetikus) Sziklai István (1954-) (fül-orr-gégész) Rákosy Zsuzsa (1978-) (sejtbiológus, molekuláris biológus, genetikus) Répássy Gábor (1947-) (fül-orr-gégész) Ádány Róza (1952-) (megelőző orvostan és népegészségtan szakorvos) Treszl Andrea (1974-) (molekuláris biológus)
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
elektronikus elérés
DOI
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4.

001-es BibID:BIBFORM103569
035-os BibID:(WoS)000851426500001 (Scopus)85146322280
Első szerző:Rebenku István
Cím:Taking molecular pathology to the next level : whole slide multicolor confocal imaging with the Pannoramic Confocal digital pathology scanner / István Rebenku, Ferenc A. Bartha, Tamás Katona, Barbara Zsebik, Géza Antalffy, Lili Takács, Béla Molnár, György Vereb
Dátum:2023
ISSN:1552-4922 1552-4930
Megjegyzések:Abstract The emergence and fast advance of digital pathology allows the acquisition, digital storage, interactive recall and analysis of morphology at the tissue level. When applying immunohistochemistry, it also affords the correlation of morphology with the expression of one or two specific molecule of interest. The rise of fluorescence pathology scanners expands the number of detected molecules based on multiplex labelling. The Pannoramic Confocal (created by 3DHistech, Hungary) is a first-of-the-kind digital pathology scanner that affords not only multiplexed fluorescent detection on top of conventional transmission imaging, but also confocality. We have benchmarked this scanner in terms of stability, precision, light efficiency, linearity and sensitivity. X-Y stability and relocalisation precision were well below resolution limit (?50 nm). Light throughput in confocal mode was 4-5 times higher than that of a point scanning confocal microscope, yielding similar calculated confocal intensities but with the potential for improving signal to noise ratio or scan speed. Response was linear with R2 ?0.9996. Calibrated measurements showed that using indirect labeling ? 2000 molecules per cell could be well detected and imaged on the cell surface. Both standard-based and statistical post-acquisition flatfield corrections are implemented. We have also measured the point spread function (PSF) of the instrument. The dimensions of the PSF are somewhat larger and less symmetric than of the theoretical PSF of a conventional CLSM, however, the spatial homogeneity of these parameters allows for obtaining a specific system PSF for each optical path and using it for optional on-the-fly deconvolution. In conclusion, the Pannoramic Confocal provides sensitive, quantitative widefield and confocal detection of multiplexed fluorescence signals, with optical sectioning and 3D reconstruction, in addition to brightfield transmission imaging. High speed scanning of large samples, analysis of tissue heterogeneity, and detection of rare events open up new ways for quantitatively analyzing tissue sections, organoid cultures or large numbers of adherent cells.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
3-dimensional virtual slide
deconvolution
digital pathology
multispectral fluorescence imaging
widefield aperture correlated confocal imaging
Megjelenés:Cytometry Part A. - 103 : 3 (2023), p. 198-207. -
További szerzők:Bartha Ferenc Katona Tamás (1991-) (informatikus) Zsebik Barbara (1977-) (biofizikus) Antalffy Géza Takács Lili (1969-) (szemész) Molnár Béla Vereb György (1965-) (biofizikus, orvos)
Pályázati támogatás:GINOP-2.2.1-15-2017-00072
GINOP
OTKA K135938
OTKA
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DOI
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5.

001-es BibID:BIBFORM041645
035-os BibID:PMID:15221868
Első szerző:Treszl Andrea (molekuláris biológus)
Cím:Extra copies of c-myc are more pronounced in nodular melanomas than in superficial spreading melanomas as revealed by fluorescence in situ hybridisation / Andrea Treszl, Róza Ádány, Zsuzsa Rákosy, László Kardos, Ágnes Bégány, Katalin Gilde, Margit Balázs
Dátum:2004
ISSN:0196-4763
Megjegyzések:Amplification of c-myc is a common genetic alteration and associated with a poor prognosis in a variety of cancers. Extra copies of the gene have been found in large numbers of melanoma metastases, but only few primary tumours have been studied. We investigated the c-myc copy number alterations in two different subtypes of primary melanomas with different biological behaviours. METHODS: Fluorescence in situ hybridisation was performed using c-myc and centromeric 8 (C8) probes on 68 lesions (28 nodular melanomas [NMs], 26 superficial spreading melanomas [SSMs], and 14 metastases). To assess the ploidy pattern, copy number distribution of seven different chromosomes was also investigated. RESULTS: All tumours showed aneuploid populations for at least three chromosomes. Whereas 61% of the NMs exhibited extra c-myc copies, only 27% of SSMs showed increased gene dosage. The c-myc/C8 ratio exceeding 1.5 was significantly higher in NMs (P = 0.01). High level amplification was seen only in NMs. An elevated c-myc/C8 ratio was higher than 1.5 in only four metastases. CONCLUSION: Our data show that c-myc copy number alterations differ in the two melanoma subtypes and are associated with the advanced stage of the disease. The less frequent amplification of the c-myc gene in metastatic lesions indicates that it may play an important role in the development of an invasive potential rather than in the metastatic process.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
nodular melanoma
superficial spreading melanoma
c-myc
fluorescence in situ hybridisation
egyetemen (Magyarországon) készült közlemény
Megjelenés:Cytometry. Part B, Clinical Cytometry. - 60B : 1 (2004), p. 37-46. -
További szerzők:Ádány Róza (1952-) (megelőző orvostan és népegészségtan szakorvos) Rákosy Zsuzsa (1978-) (sejtbiológus, molekuláris biológus, genetikus) Kardos László (1970-) (megelőző orvostan és népegészségtan szakorvos) Bégány Ágnes (1954-2011) (bőrgyógyász, kozmetológus, klinikai onkológus) Gilde Katalin Balázs Margit (1952-) (sejtbiológus, molekuláris genetikus)
Internet cím:DOI
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