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001-es BibID:	BIBFORM073541
035-os BibID:	(WoS)000430668500001 (Scopus)85044666510
Első szerző:	Kecskeméti Ádám (vegyész)
Cím:	Particle-based liquid chromatographic separations in microfluidic devices / Kecskemeti Adam, Gaspar Attila
Dátum:	2018
ISSN:	0003-2670
Megjegyzések:	The research and applications of liquid chromatographic (LC) chips receive more and more attention due to the numerous advantages over the traditional and larger analytical systems, e.g. requirement for small sample and reagent volumes, fast and inexpensive analysis, dead-volume free connections and the ability to multiplex measurements. Since LC is one of the most powerful separation techniques, its miniaturization seems an obvious target of lab-on-a-chip developments. However, the common procedures used in the preparation of chromatographic columns are not well applicable at the microscopic level. Additionally, implementing sample injection of (sub)nanoliter volumes (with sensitive detection) is still a challenge.This review deals with microchips incorporating particle-based stationary phases and focuses on the lab-made and commercialized chromatographic separations discussing the particle retention methods, the designs/constructions of LC chips, the separation performances and possible applications. A survey about microfluidic chips - capable of efficient separations and high-throughput sample pretreatment - hyphenated with electrospray mass spectrometric devices to achieve sensitive detection has also been made. The merits and limitations of different commercial LC chips were compared with other published approaches, as well.
Tárgyszavak:	Természettudományok Kémiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk Microfluidics Chromatography Separation Particle Bead Review
Megjelenés:	Analytica Chimica Acta. - 1021 (2018), p. 1-19. -
További szerzők:	Gáspár Attila (1970-) (vegyész, kémikus)
Pályázati támogatás:	GINOP-2.3.2-15-2016-00008 GINOP GINOP-2.3.3-15-2016-00004 GINOP ÚNKP-17-3 Egyéb OTKA-111932 OTKA
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001-es BibID:	BIBFORM117884
035-os BibID:	(cikkazonosító)342162 (WoS)001147540500001 (Scopus)85181709291
Első szerző:	Nagy Cynthia (1994-)
Cím:	CZE-MS peptide mapping : To desalt or not to desalt? / Cynthia Nagy, Melinda Andrasi, Ruben Szabo, Attila Gaspar
Dátum:	2024
ISSN:	0003-2670
Megjegyzések:	Background: In "shotgun" approaches involving high-performance liquid chromatography or capillary zone electrophoresis (CZE), matrix removal prior to sample analysis is considered as an indispensable tool. Despite the fact that CZE offers a high tolerance towards salts, most publications reported on the use of desalting. There seems to be no clear consensus on the utilization of desalting in the CZE-MS community, most probably due to the absence of works addressing the comparison of desalted and non-desalted digests. Our aim was to fill this research gap using protein samples of varying complexity in different sample matrices. Results: First, standard protein digests were analyzed to build the knowledge on the effect of sample clean-up by solid-phase extraction (SPE) pipette tips and the possible stacking phenomena induced by different sample matrices. Desalting led to a somewhat altered peptide profile, the procedure affected mostly the hydrophilic peptides (although not to a devastating extent). Nevertheless, desalting samples allowed remarkable stacking efficiency owing to their low-conductivity sample background, enabling a so-called field-amplified sample stacking phenomenon. Non-desalted samples also produced a stacking event, the mechanism of which is based on transient-isotachophoresis due to the presence of high-mobility ions in the digestion buffer itself. Adding either extra ammonium ions or acetonitrile into the non-desalted digests enhanced the stacking efficiency. A complex sample (yeast cell lysate) was also analyzed with the optimal conditions, which yielded similar tendencies. Significance: Based on these results, we propose that sample clean-up in the bottom-up sample preparation process prior to CZE-MS analysis can be omitted. The preclusion of desalting can even enhance detection sensitivity, separation efficiency or sequence coverage.
Tárgyszavak:	Természettudományok Kémiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk Bottom-up proteomics Capillary zone electrophoresis Desalting Mass spectrometry Peptide mapping
Megjelenés:	Analytica Chimica Acta. - 1288 (2024), p. 1-9. -
További szerzők:	Andrási Melinda (1979-) (gyógyszerész) Szabó Ruben (1998-) Gáspár Attila (1970-) (vegyész, kémikus)
Pályázati támogatás:	K-142134 Egyéb ÚNKP-22-3-II Egyéb
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001-es BibID:	BIBFORM103039
035-os BibID:	(WOS)000522084100009 (Scopus)85080151996
Első szerző:	Nagy Cynthia (1994-)
Cím:	Fabrication of immobilized enzyme reactors with pillar arrays into polydimethylsiloxane microchip / Cynthia Nagy, Adam Kecskemeti, Attila Gaspar
Dátum:	2020
ISSN:	0003-2670
Megjegyzések:	This paper demonstrates the design, efficiency and applicability of a simple and inexpensive microfluidic immobilized enzymatic reactor (IMER) for rapid protein digestion. The high surface-to-volume ratio (S/V) of the reactor was achieved by forming pillars in the channel. It was found that pillar arrays including dimensions of 40 mu m x 40 mu m as pillar diameter and interpillar distance can provide both relatively high S/V and flow rate in the PDMS chip, the fabrication of which was performed by means of soft lithography using average research laboratory infrastructure. CZE peptide maps of IMER-based digestions were compared to peptide maps obtained from standard in-solution digestion of proteins. The peak patterns of the electropherograms and the identified proteins were similar, however, digestion with the IMER requires less than 10 min, while in-solution digestion takes 16 h.
Tárgyszavak:	Természettudományok Kémiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk Immobilization Digestion Peptide mapping Enzyme reactor Array of pillars poly(dimethylsiloxane)
Megjelenés:	Analytica Chimica Acta. - 1108 (2020), p. 70-78. -
További szerzők:	Kecskeméti Ádám (1992-) (vegyész) Gáspár Attila (1970-) (vegyész, kémikus)
Pályázati támogatás:	GINOP-2.3.2-15-2016-00008 GINOP GINOP-2.3.3-15-2016-00004 GINOP NKFIH-K127931 Egyéb
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001-es BibID:	BIBFORM046601
Első szerző:	Posta József (vegyész, analitikus)
Cím:	On-line separation and preconcentration of chromium species in seawater / J. Posta, A. Alimonti, F. Petrucci, S. Caroli
Dátum:	1996
ISSN:	0003-2670
Tárgyszavak:	Természettudományok Kémiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	Analytica Chimica Acta. - 325 : 3 (1996), p. 185-193. -
További szerzők:	Alimonti, A. Petrucci, F. Caroli, S.
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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