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001-es BibID:	BIBFORM008925
Első szerző:	Bágyi Kinga (fogszakorvos)
Cím:	Role of pathogenic oral flora in postoperative pneumonia following brain surgery / Bágyi Kinga, Haczku Angela, Márton Ildikó, Szabó Judit, Gáspár Attila, Andrási Melinda, Varga Imre, Tóth Judit, Klekner Álmos
Dátum:	2009
Megjegyzések:	Post-operative pulmonary infection often appears to result from aspiration of pathogens colonizing the oral cavity. It was hypothesized that impaired periodontal status and pathogenic oral bacteria significantly contribute to development of aspiration pneumonia following neurosurgical operations. Further, the prophylactic effects of a single dose preoperative cefazolin on the oral bacteria were investigated. Methods: A matched cohort of 18 patients without postoperative lung complications was compared to 5 patients who developed pneumonia within 48 hours after brain surgery. Patients waiting for elective operation of a single brain tumor underwent dental examination and saliva collection before surgery. Bacteria from saliva cultures were isolated and periodontal disease was scored according to type and severity. Patients received 15 mg/kg cefazolin intravenously at the beginning of surgery. Serum, saliva and bronchial secretion were collected promptly after the operation. The minimal inhibitory concentrations of cefazolin regarding the isolated bacteria were determined. The actual antibiotic concentrations in serum, saliva and bronchial secretion were measured by capillary electrophoresis upon completion of surgery. Bacteria were isolated again from the sputum of postoperative pneumonia patients. Results: The number and severity of coexisting periodontal diseases were significantly greater in patients with postoperative pneumonia in comparison to the control group (p = 0.031 and p = 0.002, respectively). The relative risk of developing postoperative pneumonia in high periodontal score patients was 3.5 greater than in patients who had low periodontal score (p < 0.0001). Cefazolin concentration in saliva and bronchial secretion remained below detectable levels in every patient. Conclusion: Presence of multiple periodontal diseases and pathogenic bacteria in the saliva are important predisposing factors of postoperative aspiration pneumonia in patients after brain surgery. The low penetration rate of cefazolin into the saliva indicates that its prophylactic administration may not be sufficient to prevent postoperative aspiration pneumonia. Our study suggests that dental examination may be warranted in order to identify patients at high risk of developing postoperative respiratory infections.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk pneumonia egyetemen (Magyarországon) készült közlemény
Megjelenés:	BMC Infectious Diseases. - 9 (2009), p. 104-113. -
További szerzők:	Haczku Angela Márton Ildikó (1954-) (fogszakorvos) Szabó Judit (1963-) (szakorvos, klinikai mikrobiológus) Gáspár Attila (1970-) (vegyész, kémikus) Andrási Melinda (1979-) (gyógyszerész) Varga Imre (1960-) (tüdőgyógyász) Tóth Judit (1958-) (onkológus szakorvos) Klekner Álmos (1970-) (idegsebész)
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001-es BibID:	BIBFORM074742
Első szerző:	Fidler Gábor (molekuláris biológus, genetikus)
Cím:	Validation of a simplex PCR assay enabling reliable identification of clinically relevant Candida species / Fidler Gabor, Leiter Eva, Kocsube Sandor, Biro Sandor, Paholcsek Melinda
Dátum:	2018
ISSN:	1471-2334 1471-2334
Megjegyzések:	Background: Fungal bloodstream infections (BSI) may be serious and are associated with drastic rise in mortality and health care costs. Candida spp. are the predominant etiological agent of fungal sepsis. The prompt and species-level identification of Candida may influence patient outcome and survival. The aim of this study was to develop and evaluate the CanTub-simplex PCR assay coupled with Tm calling and subsequent high resolution melting (HRM) analysis to barcode seven clinically relevant Candida species. Methods: Efficiency, coefficient of correlation and the limit of reliable detection were estimated on purified Candida EDTA-whole blood (WB) reference panels seeded with Candida albicans, Candida glabrata, Candida parapsilosis, Candida tropicalis, Candida krusei, Candida guilliermondii, Candida dubliniensis cells in a 6-log range. Discriminatory power was measured on EDTA-WB clinical panels on three different PCR platforms; LightCycler?96, LightCycler? Nano, LightCycler? 2.0. Inter- and intra assay consistencies were also calculated. Results: The limit of reliable detection proved to be 0.2?2 genomic equivalent and the method was reliable on broad concentration ranges (106 ?10 CFU) providing distinctive melting peaks and characteristic HRM curves. The diagnostic accuracy of the discrimination proved to be the best on Roche LightCycler?2.0 platform. Repeatability was tested and proved to be % C.V.: 0.14 ? 0.06 on reference- and % C.V.: 0.14 ? 0.02 on clinical-plates accounting for a very high accuracy. Reproducibility was % C.V.: 0.11 between reference- and % C.V.: 0.12between clinicalpanels which is highly acceptable. Conclusion: Our assay demonstrates recent advances on Tm calling and HRM analysis for the molecular identification of relevant Candida species. This unique, simplex PCR assay may be capable to outperform conventional phenotypic methods by reducing time and providing accurate and reliable results directly from blood (2 h) or from whole blood culture bottles (12?24 h).
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban Candida High resolution melting Tm calling Species level identification Simplex PCR
Megjelenés:	BMC Infectious Diseases. - 18 : 393 (2018), p. 1-13. -
További szerzők:	Leiter Éva (1976-) (biológus) Kocsubé Sándor Biró Sándor (1949-) (molekuláris genetikus) Paholcsek Melinda (1984-) (molekuláris biológus, genetikus)
Pályázati támogatás:	GINOP-2.3.2-15-2016-00042 GINOP
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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