CCL

Összesen 22 találat.
#/oldal:
Részletezés:
Rendezés:

1.

001-es BibID:BIBFORM074251
035-os BibID:(cikkazonosító)744 (WOS)000429953200001 (Scopus)85045321936
Első szerző:Bakti Fruzsina
Cím:Heavy metal induced expression of PcaA provides cadmium tolerance to Aspergillus fumigatus and supports its virulence in the Galleria mellonella model / Fruzsina Bakti, Christoph Sasse, Thorsten Heinekamp, István Pócsi, Gerhard H. Braus
Dátum:2018
ISSN:1664-302X
Megjegyzések:Most of the metal transporters in Aspergillus fumigatus are yet uncharacterized. Their role in fungal metabolism and virulence remains unclear. This paper describes the novel PIB-type cation ATPase PcaA, which links metal homeostasis and heavy metal tolerance in the opportunistic human pathogen A. fumigatus. The protein possesses conserved ATPase motif and shares 51% amino acid sequence identity with the Saccharomyces cerevisiae cadmium exporter Pca1p. A pcaA deletion, an overexpression and a gfp-pcaA complementation strain of A. fumigatus were constructed and their heavy metal susceptibilities were studied. The pcaA knock out strain showed drastically decreased cadmium tolerance, however, its growth was not affected by the exposure to high concentrations of copper, iron, zinc, or silver ions. Although the lack of PcaA had no effect on copper adaption, we demonstrated that not only cadmium but also copper ions are able to induce the transcription of pcaA in A. fumigatus wild type Af293. Similarly, cadmium and copper ions could induce the copper exporting ATPase crpA. These data imply a general response on the transcriptomic level to heavy metals in A. fumigatus through the induction of detoxification systems. Confocal microscopy of the gfp-pcaA complementation strain expressing functional GFP-PcaA supports the predicted membrane localization of PcaA. The GFP-PcaA fusion protein is located in the plasma membrane of A. fumigatus in the presence of cadmium ions. Virulence assays support a function of PcaA for virulence of A. fumigatus in the Galleria mellonella wax moth larvae model, which might be linked to the elimination of reactive oxygen species.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
gene cluster
biosynthetic
Megjelenés:Frontiers in Microbiology. - 9 (2018), p. [11]. -
További szerzők:Sasse, Christoph Heinekamp, Thorsten Pócsi István (1961-) (vegyész) Braus, Gerhard
Pályázati támogatás:TÁMOP-4.2.4B/2-11/1-2012-0001
TÁMOP
Internet cím:DOI
Intézményi repozitóriumban (DEA) tárolt változat
Borító:

2.

001-es BibID:BIBFORM103776
035-os BibID:(cikkazonosító)1000688 (WOS)000871611300001 (Scopus)85138178796
Első szerző:Farkas Zsuzsa
Cím:Probabilistic modeling and risk characterization of the chronic aflatoxin M1 exposure of Hungarian consumers / Zsuzsa Farkas, Kata Kerekes, Árpád Ambrus, Miklós Süth, Ferenc Peles, Tünde Pusztahelyi, István Pócsi, Attila Nagy, Péter Sipos, Gabriella Miklós, Anna Lőrincz10, Szilveszter Csorba, Ákos Bernard Jóźwiak
Dátum:2022
ISSN:1664-302X
Megjegyzések:Aflatoxin contamination can appear in various points of the food chain. If animals are fed with contaminated feed, AFB1 is transformed?among others?to aflatoxin M1 (AFM1) metabolite. AFM1 is less toxic than AFB1, but it is still genotoxic and carcinogenic and it is present in raw and processed milk and all kinds of milk products. In this article, the chronic exposure estimation and risk characterization of Hungarian consumers are presented, based on the AFM1 contamination of milk and dairy products, and calculated with a probabilistic method, the two-dimensional Monte-Carlo model. The calculations were performed using the R plugin (mc2d package) integrated into the KNIME (Konstanz Information Miner) software. The imulations were performed using data from the 2018?2020 food consumption survey. The AFM1 analytical data were derived from the Hungarian monitoring survey and 1,985 milk samples were analyzed within the framework of the joint project of the University of Debrecen and the National Food Chain Safety Office of Hungary (NÉBIH). Limited AFM1 concentrations were available for processed dairy products; therefore, a database of AFM1 processing factors for sour milk products and various cheeses was produced based on the latest literature data, and consumer exposure was calculated with the milk equivalent of the consumed quantities of these products. For risk characterization, the calculation of hazard index (HI), Margin of Exposure, and the hepatocellular carcinoma incidence were used. The results indicate that the group of toddlers that consume a large amount of milk and milk products are exposed to a certain level of health risk. The mean estimated daily intake of toddlers is in the range of 0.008?0.221ngkg?1 bw day?1; the 97.5th percentile exposure of toddlers is between 0.013ngkg?1 bw day?1 and 0.379ngkg?1 bw day?1, resulting in a HI above 1. According to our study, the exposure of older age groups does not pose an emergent health risk. Nevertheless, the presence of carcinogenic compounds should be kept to a minimum in the whole population.
Tárgyszavak:Agrártudományok Élelmiszertudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
AFM1
mycotoxin exposure assessment
probabilistic method
Megjelenés:Frontiers in Microbiology. - 13 (2022), p. 1-12. -
További szerzők:Kerekes Kata Ambrus Árpád Süth Miklós Peles Ferenc (1979-) (mikrobiológia, élelmiszer-mikrobiológia, minőségügy) Pusztahelyi Tünde (1969-) (biológus, angol-magyar szakfordító) Pócsi István (1961-) (vegyész) Nagy Attila Sipos Péter (1975-) (agrármérnök) Miklós Gabriella Lőrincz Anna Csorba Szilveszter Jóźwiak Ákos
Pályázati támogatás:2018-1.2.1-NKP-2018-00002
Egyéb
Internet cím:Szerző által megadott URL
DOI
Intézményi repozitóriumban (DEA) tárolt változat
Borító:

3.

001-es BibID:BIBFORM089163
035-os BibID:(cikkazonosító)600983 (WOS)000589965500001 (Scopus)85096189197
Első szerző:Gao, Ying
Cím:Induction of New Lactam Derivatives From the Endophytic Fungus Aplosporella javeedii Through an OSMAC Approach / Ying Gao, Fabian Stuhldreier, Laura Schmitt, Sebastian Wesselborg, Zhiyong Guo, Kun Zou, Attila Mándi, Tibor Kurtán, Zhen Liu, Peter Proksch
Dátum:2020
ISSN:1664-302X
Megjegyzések:Fermentation of the endophytic fungus Aplosporella javeedii on solid rice medium in presence of either 3.5% NaNO3 or 3.5% monosodium glutamate caused a significant change of the fungal metabolite pattern compared to fungal controls grown only on rice. Chemical investigation of the former fungal extracts yielded 11 new lactam derivatives, aplosporellins A-K (2-12), in addition to the known compound, pramanicin A (1). All of these compounds were not detected when the fungus was grown on rice medium without these activators thereby indicating the power of this OSMAC approach. The structures of the new compounds were elucidated by one- and two- dimensional NMR spectroscopy, DFT-NMR calculations and by mass spectrometry as well as by comparison with the literature whereas the absolute configuration of the lactam core was determined by TDDFT-ECD and OR calculations. Pramanicin A (1) showed strong cytotoxicity against human lymphoma (Ramos) and leukemia (Jurkat J16) cells with IC50 values of 4.7 and 4.4 mM, respectively. Mechanistic studies indicated that 1 activates caspase-3 and induces apoptotic cell death.
Tárgyszavak:Természettudományok Kémiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Aplosporella javeedii
lactam derivatives
OSMAC approach
DFT-NMR
TDDFT-ECD
OR calculations
apoptosis
Megjelenés:Frontiers in Microbiology. - 11 (2020), p. 1-13. -
További szerzők:Stuhldreier, Fabian Schmitt, Laura Wesselborg, Sebastian Guo, Zhi-Yong Zou, Kun Mándi Attila (1981-) (vegyész, német szakfordító) Kurtán Tibor (1973-) (vegyész, angol szakfordító) Liu, Zhen Proksch, Peter
Pályázati támogatás:GINOP-2.3.2-15-2016-00008
GINOP
NKFI K120181
Egyéb
Internet cím:Szerző által megadott URL
DOI
Intézményi repozitóriumban (DEA) tárolt változat
Borító:

4.

001-es BibID:BIBFORM093531
035-os BibID:(cikkazonosító)680420 (WOS)000657282100001 (Scopus)85107225932
Első szerző:Karaffa Erzsébet Mónika (mikrobiológus, egyetemi tanár)
Cím:Carbon-source dependent interplay of copper and manganese ions modulates the morphology and itaconic acid production in Aspergillus terreus / Erzsébet Sándor, István S. Kolláth, Erzsébet Fekete, Vivien Bíró, Michel Flipphi, Béla Kovács, Christian P. Kubicek, Levente Karaffa
Dátum:2021
ISSN:1664-302X
Megjegyzések:The effects of the interplay of copper (II) and manganese (II) ions on growth, morphology and itaconic acid formation was investigated in a high-producing strain of Aspergillus terreus (NRRL1960), using carbon sources metabolized either mainly via glycolysis (D-glucose, D-fructose) or primarily via the pentose phosphate shunt (D-xylose, L-arabinose). Limiting Mn2+ concentration in the culture broth is indispensable to obtain high itaconic acid yields, while in the presence of higher Mn2+ concentrations yield decreases and biomass formation is favored. However, this low yield in the presence of high Mn2+ ion concentrations can be mitigated by increasing the Cu2+ concentration in the medium when D-glucose or D-fructose is the growth substrate, whereas this effect was at best modest during growth on D-xylose or -arabinose. A. terreus displays a high tolerance to Cu2+ which decreased when Mn2+ availability became increasingly limiting. Under such conditions biomass formation on D-glucose or D-fructose could be sustained at concentrations up to 250 mg L-1 Cu2+, while D-xylose- or L-arabinose supported growth was fully inhibited already at 100 mg L-1, while on D-xylose- or L-arabinose biomass formation was completely inhibited at 100 mg L-1. High (>75%) specific molar itaconic acid yields always coincided with an "overflow-associated" morphology, characterized by small compact pellets (<250 mm diameter) and short chains of "yeast-like" cells that exhibit increased diameters relative to the elongated cells in growing filamentous hyphae. At low concentrations (?1 mg L-1) of Cu2+ ions, manganese deficiency did not prevent filamentous growth. Mycelial- and cellular morphology progressively transformed into the typical overflow-associated one when external Cu2+ concentrations increased, irrespective of the available Mn2+. Our results indicate that copper ions are relevant for overflow metabolism and should be considered when optimizing itaconic acid fermentation in A. terreus.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Aspergillus terreus
Manganese(II) ions
Copper(II) ions
itaconic acid overflow
Pentose, hexose
Mycelial pellets
divalent cation antagonism
Megjelenés:Frontiers in Microbiology. - 12 (2021), p. 1-14. -
További szerzők:Kolláth István Sándor (biomérnök) Fekete Erzsébet (1975-) (biotechnológus, egyetemi tanár) Bíró Vivien (1994-)c(mikrobiológus) Flipphi, Michel (1964-) Kovács Béla (1963-) (okleveles vegyész, angol szakfordító) Kubicek, Christian P. Karaffa Levente (1971-) (biotechnológus, egyetemi tanár)
Internet cím:Szerző által megadott URL
DOI
Intézményi repozitóriumban (DEA) tárolt változat
Borító:

5.

001-es BibID:BIBFORM105401
035-os BibID:(cikkazonosító)1003709 (WoS)000868652700001 (Scopus)85139183146
Első szerző:Kocsis Beatrix (molekuláris biológus)
Cím:Functional analysis of the bZIP-type transcription factors AtfA and AtfB in Aspergillus nidulans / Beatrix Kocsis, Mi-Kyung Lee, Jae-Hyuk Yu, Tibor Nagy, Lajos Daróczi, Gyula Batta, István Pócsi, Éva Leiter
Dátum:2022
ISSN:1664-302X
Megjegyzések:Transcription factors (TFs) with the basic leucin zipper domain are key elements of the stress response pathways in filamentous fungi. In this study, we functionally characterized the two bZIP type TFs AtfA and AtfB by deletion (Delta) and overexpression (OE) of their encoding genes in all combination: Delta atfA, Delta atfB, Delta atfA Delta atfB, Delta atfAatfBOE, Delta atfBatfAOE, atfAOE, atfBOE and atfAOEatfBOE in Aspergillus nidulans. Based on our previous studies, Delta atfA increased the sensitivity of the fungus to oxidative stress mediated by menadione sodium bisulfite (MSB) and tert-butylhydroperoxide (tBOOH), while Delta atfB was not sensitive to any oxidative stress generating agents, namely MSB, tBOOH and diamide at all. Contrarily, the Delta atfB mutant was sensitive to NaCl, but tolerant to sorbitol. Overexpression of atfB was able to compensate the MSB sensitivity of the Delta atfA mutant. Heavy metal stress elicited by CdCl2 reduced diameter of the atfBOE and atfAOEatfBOE mutant colonies to about 50% of control colony, while the cell wall stress generating agent CongoRed increased the tolerance of the Delta atfA mutant. When we tested the heat stress sensitivity of the asexual spores (conidiospores) of the mutants, we found that conidiospores of Delta atfAatfBOE and Delta atfBatfAOE showed nearly 100% tolerance to heat stress. Asexual development was negatively affected by Delta atfA, while atfAOE and atfAOE coupled with Delta atfB increased the number of conidiospores of the fungus approximately 150% compared to the control. Overexpression of atfB led to a 25% reduction in the number of conidiospores, but increased levels of abaA mRNA and size of conidiospores. Sexual fruiting body (cleistothecium) formation was diminished in the Delta atfA and the Delta atfA Delta atfB mutants, while relatively elevated in the Delta atfB and the Delta atfBatfAOE mutants. Production of the mycotoxin sterigmatocystin (ST) was decreased to undetectable levels in the Delta atfA mutant, yet ST production was restored in the Delta atfA Delta atfB mutant, suggesting that Delta atfB can suppress ST production defect caused by Delta atfA. Levels of ST were also significantly decreased in the Delta atfAatfBOE, Delta atfBatfAOE and atfAOEatfBOE mutants.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
aspergillus nidulans
environmental stress
conidiospore
cleistothecium
sterigmatocystin
Megjelenés:Frontiers in Microbiology. - 13 (2022), p. 1-9. -
További szerzők:Lee Mi-Kyung Yu Jae-Hyuk Nagy Tibor (1988-) (vegyész) Daróczi Lajos (1965-) (fizikus) Batta Gyula (1979-) (biológus) Pócsi István (1961-) (vegyész) Leiter Éva (1976-) (biológus)
Pályázati támogatás:NKFIH
Egyéb
Internet cím:Szerző által megadott URL
DOI
Intézményi repozitóriumban (DEA) tárolt változat
Borító:

6.

001-es BibID:BIBFORM079798
035-os BibID:(cikkazonosító)1589 (WOS)000474482200002 (Scopus)85069454227
Első szerző:Kolláth István Sándor (biomérnök)
Cím:Manganese Deficiency Is Required for High Itaconic Acid Production From D-Xylose in Aspergillus terreus / István S. Kolláth, Ákos P. Molnár, Áron Soós, Erzsébet Fekete, Erzsébet Sándor, Béla Kovács, Christian P. Kubicek, Levente Karaffa
Dátum:2019
ISSN:1664-302X
Megjegyzések:Itaconic acid is used as a bio-based, renewable building block in the polymer industry. It is produced by submerged fermentations of the filamentous fungus Aspergillus terreus from molasses or starch, but research over the efficient utilization of non-food, lignocellulosic plant biomass is soaring. The objective of this study was to test whether the application of two key cultivation parameters for obtaining itaconic acid from D-glucose in high yields ? Mn2+ ion deficiency and high concentration of the carbon source ? would also occur on D-xylose, the principal monomer of lignocellulose. To this end, a carbon and energy balance for itaconic acid formation was established, which is 0.83 moles/mole D-xylose. The effect of Mn2+ ions on itaconic acid formation was indeed similar to that on D-glucose and maximal yields were obtained below 3 ?g L?1 Mn2+ ions, which were, however, only 0.63 moles of itaconic acid per mole D-xylose. In contrast to the case on D-glucose, increasing D-xylose concentration over 50 g L?1 did not change the above yield. By-products such as xylitol and ?-ketoglutarate were found, but in total they remained below 2% of the concentration of D-xylose. Mass balance of the fermentation with 110 g L?1 D-xylose revealed that >95% of the carbon from D-xylose was accounted as biomass, itaconic acid, and the carbon dioxide released in the last step of itaconic acid biosynthesis. Our data show that the efficiency of biomass formation is the critical parameter for itaconic acid yield from D-xylose under otherwise optimal conditions.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Aspergillus terreus
D-xylose
itaconic acid
fermentation
specific yield
volumetric yield
Megjelenés:Frontiers in Microbiology. - 10 (2019), p. 1-10. -
További szerzők:Molnár Ákos Péter (1988-) (okleveles biomérnök) Soós Áron (1990-) (élelmiszerbiztonsági és -minőségi mérnök) Fekete Erzsébet (1975-) (biotechnológus, egyetemi tanár) Karaffa Erzsébet Mónika (1972-) (mikrobiológus, egyetemi tanár) Kovács Béla (1963-) (okleveles vegyész, angol szakfordító) Kubicek, Christian P. Karaffa Levente (1971-) (biotechnológus, egyetemi tanár)
Pályázati támogatás:EFOP-3.6.1-16-2016-00022
EFOP
GINOP-2.3.2-15-2016-00008
GINOP
NKFIA-KH-129602
Egyéb
NKFIA-NN-128867
Egyéb
NKFIA-NN-116519
Egyéb
Internet cím:Szerző által megadott URL
DOI
Intézményi repozitóriumban (DEA) tárolt változat
Borító:

7.

001-es BibID:BIBFORM116975
035-os BibID:(cikkazonosító)1260166 (WoS)001143637400001 (Scopus)85182399795
Első szerző:Krishnan, S. Vipin
Cím:Fusarium biocontrol : antagonism and mycotoxin elimination by lactic acid bacteria / S. Vipin Krishnan, K. Madhavan Nampoothiri, Anandhu Suresh, Nguyen Thuy Linh, P. A. Balakumaran, István Pócsi, Tünde Pusztahelyi
Dátum:2024
ISSN:1664-302X
Megjegyzések:Mycotoxins produced by Fusarium species are secondary metabolites with low molecular weight formed by filamentous fungi generally resistant to different environmental factors and, therefore, undergo slow degradation. Contamination by Fusarium mycotoxins in cereals and millets is the foremost quality challenge the food and feed industry faces across the globe. Several types of chemical preservatives are employed in the mitigation process of these mycotoxins, and they help in long-term storage; however, chemical preservatives can be used only to some extent, so the complete elimination of toxins from foods is still a herculean task. The growing demand for green-labeled food drives to evade the use of chemicals in the production processes is getting much demand. Thus, the biocontrol of food toxins is important in the developing food sector. Fusarium mycotoxins are world-spread contaminants naturally occurring in commodities, food, and feed. The major mycotoxins Fusarium species produce are deoxynivalenol, fumonisins, zearalenone, and T2/HT2 toxins. Lactic acid bacteria (LAB), generally regarded as safe (GRAS), is a well-explored bacterial community in food preparations and preservation for ages. Recent research suggests that LAB are the best choice for extenuating Fusarium mycotoxins. Apart from Fusarium mycotoxins, this review focuses on the latest studies on the mechanisms of how LAB effectively detoxify and remove these mycotoxins through their various bioactive molecules and background information of these molecules.
Tárgyszavak:Agrártudományok Élelmiszertudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
lactic acid bacteria
Fusarium
mycotoxin
antagonism
biocontrol bioactive
biocontrol
mycotoxins
Megjelenés:Frontiers in Microbiology. - 14 (2024), p. 1-17. -
További szerzők:Nampoothiri K. Madhavan Suresh, Anandhu Nguyen Thuy Linh Balakumaran P. A. Pócsi István (1961-) (vegyész) Pusztahelyi Tünde (1969-) (biológus, angol-magyar szakfordító)
Pályázati támogatás:2019-2.1.13-TÉT_IN-2020-00056
Egyéb
TKP2021-NKTA-32
Egyéb
Internet cím:Szerző által megadott URL
DOI
Intézményi repozitóriumban (DEA) tárolt változat
Borító:

8.

001-es BibID:BIBFORM106701
035-os BibID:(cikkazonosító)1085891 (WoS)000929068300001 (Scopus)85147698175
Első szerző:Loi, Martina
Cím:Advanced mycotoxin control and decontamination techniques in view of an increased aflatoxin risk in Europe due to climate change / Martina Loi, Antonio F. Logrieco, Tünde Pusztahelyi, Éva Leiter, László Hornok, István Pócsi
Dátum:2023
ISSN:1664-302X
Megjegyzések:Aflatoxins are toxic secondary metabolites produced by Aspergillus spp. found in staple food and feed commodities worldwide. Aflatoxins are carcinogenic, teratogenic, and mutagenic, and pose a serious threat to the health of both humans and animals. The global economy and trade are significantly affected as well. Various models and datasets related to aflatoxins in maize have been developed and used but have not yet been linked. The prevention of crop loss due to aflatoxin contamination is complex and challenging. Hence, the set-up of advanced decontamination is crucial to cope with the challenge of climate change, growing population, unstable political scenarios, and food security problems also in European countries. After harvest, decontamination methods can be applied during transport, storage, or processing, but their application for aflatoxin reduction is still limited. Therefore, this review aims to investigate the effects of environmental factors on aflatoxin production because of climate change and to critically discuss the present-day and novel decontamination techniques to unravel gaps and limitations to propose them as a tool to tackle an increased aflatoxin risk in Europe
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
aflatoxin
climate change
models
mycotoxin control
decontamination techniques
Megjelenés:Frontiers in Microbiology. - 13 (2023), p. 1-18. -
További szerzők:Logrieco, Antonio F. Pusztahelyi Tünde (1969-) (biológus, angol-magyar szakfordító) Leiter Éva (1976-) (biológus) Hornok László Pócsi István (1961-) (vegyész)
Pályázati támogatás:2018-1.2.1-NKP-2018- 00002
Egyéb
TKP2021-EGA-20 810
Egyéb
K142801
Egyéb
No 952337
Egyéb
Internet cím:Szerző által megadott URL
DOI
Intézményi repozitóriumban (DEA) tárolt változat
Borító:

9.

001-es BibID:BIBFORM116764
035-os BibID:(cikkazonosító)1294771 (WoS)001122459000001 (Scopus)85179311017
Első szerző:Miklós Máté (biológus)
Cím:Environmental bacteria increase population growth of hydra at low temperature / Máté Miklós, Karolina Cseri, Levente Laczkó, Gábor Kardos, Sebastian Fraune, Jácint Tökölyi
Dátum:2023
ISSN:1664-302X
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Megjelenés:Frontiers in Microbiology. - 14 (2023), p. 1-14. -
További szerzők:Cseri Karolina (1985-) (molekuláris biológus) Laczkó Levente (1992-) (biológus) Kardos Gábor (1974-) (szakorvos, klinikai mikrobiológus) Fraune, Sebastian Tökölyi Jácint (1984-) (biológus)
Pályázati támogatás:MTA-DE Lendület
MTA
Élettudományok - Biológiai tudományok
Internet cím:Szerző által megadott URL
DOI
Intézményi repozitóriumban (DEA) tárolt változat
Borító:

10.

001-es BibID:BIBFORM085057
035-os BibID:(cikkazonosító)957 (WOS)000539331800001 (Scopus)85085883554
Első szerző:Nagy Fruzsina (molekuláris biológus)
Cím:In vitro and in vivo effect of exogenous farnesol exposure against Candida auris / Fruzsina Nagy, Eszter Vitális, Ágnes Jakab, Andrew M. Borman, Lajos Forgács, Zoltán Tóth, László Majoros, Renátó Kovács
Dátum:2020
ISSN:1664-302X
Megjegyzések:The spreading of multidrug-resistant Candida auris is considered as an emerging global health threat. The number of effective therapeutic regimens is strongly limited; therefore, development of novel strategies is needed. Farnesol is a quorum-sensing molecule with a potential antifungal and/or adjuvant effect; it may be a promising candidate in alternative treatment against Candida species including C. auris. To examine the effect of farnesol on C. auris, we performed experiments focusing on growth, biofilm production ability, production of enzymes related to oxidative stress, triazole susceptibility and virulence. Concentrations ranging from 100 to 300 uM farnesol caused a significant growth inhibition against C. auris planktonic cells for 24 hours (p<0.01-0.05). Farnesol treatment showed a concentration dependent inhibition in terms of biofilm forming ability of C. auris; however, it did not inhibit significantly the biofilm development at 24 hours. Nevertheless, the metabolic activity of adhered farnesol pre-exposed cells (75 uM) was significantly diminished at 24 hours depending on farnesol treatment during biofilm formation (p<0.001-0.05). Moreover, 300 uM farnesol exerted a marked decrease in metabolic activity against one-day-old biofilms between 2 and 24 hours (p<0.001). Farnesol increased the production of reactive species remarkably, as revealed by 2',7'-dichlorofluorescein (DCF) assay (3.96?0.89 [nmol DCF (OD640)-1] and 23.54?4.51 [nmol DCF (OD640)-1] for untreated cells and farnesol exposed cells, respectively; p<0.001). This was in line with increased superoxide dismutase level (85.69?5.42 [munit (mg protein)-1] and 170.11?17.37 [munit (mg protein)-1] for untreated cells and farnesol exposed cells, respectively; p<0.001), but the catalase level remained statistically comparable between treated and untreated cells (p>0.05). Concerning virulence-related enzymes, exposure to 75 uM farnesol did not influence phospholipase or aspartic proteinase activity (p>0.05). The interaction between fluconazole, intraconazole, voriconazole, posaconazole, isavuconazole and farnesol showed clear synergism (FICI ranges from 0.038 to 0.375) against one-day-old biofilms. Regarding in vivo experiments, daily 75 uM farnesol treatment decreased the fungal burden in an immuncompromised murine model of disseminated candidiasis, especially in case of inocula pre-exposed to farnesol (p<0.01). In summary, farnesol shows a promising therapeutic or adjuvant potential in traditional or alternative therapies such as catheter lock therapy.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Megjelenés:Frontiers in Microbiology. - 11 (2020), p. 1-12. -
További szerzők:Vitális Eszter (1977-) (orvos) Jakab Ágnes (1987-) (biológus) Borman, Andrew M. Forgács Lajos Tóth Zoltán (1990-) (molekuláris biológus) Majoros László (1966-) (szakorvos, klinikai mikrobiológus) Kovács Renátó László (1987-) (molekuláris biológus)
Pályázati támogatás:EFOP-3.6.3-VEKOP-16-2017-00009
EFOP
ÚNKP-19-3
Egyéb
Internet cím:Szerző által megadott URL
DOI
Intézményi repozitóriumban (DEA) tárolt változat
Borító:

11.

001-es BibID:BIBFORM082608
035-os BibID:(cikkazonosító)2861 (WOS)000504275200001 (Scopus)85077285551
Első szerző:Peles Ferenc (mikrobiológia, élelmiszer-mikrobiológia, minőségügy)
Cím:Adverse Effects, Transformation and Channeling of Aflatoxins Into Food Raw Materials in Livestock / Ferenc Peles, Péter Sipos, Zoltán Győri, Walter P. Pfliegler, Federica Giacometti, Andrea Serraino, Giampiero Pagliuca, Teresa Gazzotti, István Pócsi
Dátum:2019
ISSN:1664-302X
Megjegyzések:Aflatoxins are wide-spread harmful carcinogenic secondary metabolites produced by Aspergillus species, which cause serious feed and food contaminations and affect farm animals deleteriously with acute or chronic manifestations of mycotoxicoses. On farm, both pre-harvest and post-harvest strategies are applied to minimize the risk of aflatoxin contaminations in feeds. The great economic losses attributable to mycotoxin contaminations have initiated a plethora of research projects to develop new, effective technologies to prevent the highly toxic effects of these secondary metabolites on domestic animals and also to block the carry-over of these mycotoxins to humans through the food chain. Among other areas, this review summarizes the latest findings on the effects of silage production technologies and silage microbiota on aflatoxins, and it also discusses the current applications of probiotic organisms and microbial products in feeding technologies. After ingesting contaminated foodstuffs, aflatoxins are metabolized and biotransformed differently in various animals depending on their inherent and acquired physiological properties. These mycotoxins may cause primary aflatoxicoses with versatile, species-specific adverse effects, which are also dependent on the susceptibility of individual animals within a species, and will be a function of the dose and duration of aflatoxin exposures. The transfer of these undesired compounds from contaminated feed into food of animal origin and the aflatoxin residues present in foods become an additional risk to human health, leading to secondary aflatoxicoses. Considering the biological transformation of aflatoxins in livestock, this review summarizes (i) the metabolism of aflatoxins in different animal species, (ii) the deleterious effects of the mycotoxins and their derivatives on the animals, and (iii) the major risks to animal health in terms of the symptoms and consequences of acute or chronic aflatoxicoses, animal welfare and productivity. Furthermore, we traced the transformation and channeling of Aspergillus-derived mycotoxins into food raw materials, particularly in the case of aflatoxin contaminated milk, which represents the major route of human exposure among animal-derived foods. The early and reliable detection of aflatoxins in feed, forage and primary commodities is an increasingly important issue and, therefore, the newly developed, easy-to-use qualitative and quantitative aflatoxin analytical methods are also summarized in the review.
Tárgyszavak:Agrártudományok Élelmiszertudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Megjelenés:Frontiers in Microbiology. - 10 (2019), p. 1-26. -
További szerzők:Sipos Péter (1975-) (agrármérnök) Győri Zoltán (1948-) (vegyész) Pfliegler Valter Péter (1986-) (molekuláris biológus) Giacometti, Federica Serraino, Andrea Pagliuca, Giampiero Gazzotti, Teresa Pócsi István (1961-) (vegyész)
Pályázati támogatás:Debreceni Egyetem Biotechnológia tématerületi program
FIKP
2018-1.2.1-NKP-2018-00002
Egyéb
Internet cím:Szerző által megadott URL
DOI
Intézményi repozitóriumban (DEA) tárolt változat
Borító:

12.

001-es BibID:BIBFORM082438
035-os BibID:(cikkazonosító)2921 (WOS)000517458200001 (Scopus)85083912366
Első szerző:Pfliegler Valter Péter (molekuláris biológus)
Cím:The Aspergilli and their mycotoxins : metabolic interactions with plants and the soil biota / Valter P. Pfliegler, István Pócsi, Zoltán Győri, Tünde Pusztahelyi
Dátum:2020
ISSN:1664-302X
Megjegyzések:Species of the highly diverse fungal genus Aspergillus are well-known agricultural pests and, most importantly, producers of various mycotoxins threatening food safety worldwide. Mycotoxins are studied predominantly from the perspectives of human and livestock health. Meanwhile, their roles are far less known in nature. However, to understand the factors behind mycotoxin production, the roles of the toxins of the Aspergilli must be understood from a complex ecological perspective, taking mold-plant, mold-microbe, and mold-animal interactions into account. The Aspergilli may switch between saprophytic and pathogenic lifestyles, and the production of secondary metabolites, such as mycotoxins, may vary according to these fungal ways of life. Recent studies highlighted the complex ecological network of soil microbiotas determining the niches that Aspergilli can fill in. Interactions with the soil microbiota and soil macro-organisms determine the role of secondary metabolite production to a great extent. While, upon infection of plants, metabolic communication including fungal secondary metabolites like aflatoxins, gliotoxin, patulin, cyclopiazonic acid, ochratoxin, influences the fate of both the invader and the host. In this review, the role of mycotoxin producing Aspergillus species and their interactions in the ecosystem are discussed. We intend to highlight the complexity of the roles of the main toxic secondary metabolites as well as their fate in natural environments and agriculture, a field that still has important knowledge gaps.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Megjelenés:Frontiers in Microbiology. - 10 (2020), p. 1-45. -
További szerzők:Pócsi István (1961-) (vegyész) Győri Zoltán (1948-) (vegyész) Pusztahelyi Tünde (1969-) (biológus, angol-magyar szakfordító)
Pályázati támogatás:NKFIH 2018-1.2.1-NKP
Egyéb
OTKA K119494
OTKA
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
DOI
Borító:
Rekordok letöltése1 2 
Corvina könyvtári katalógus v8.2.27 © 2023 Monguz kft. Minden jog fenntartva.