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1.

001-es BibID:BIBFORM111540
035-os BibID:(cikkazonosító)550 (Scopus)85160562825 (WoS)000997913000001
Első szerző:Ács-Szabó Lajos (biológus)
Cím:Disruption of the Schizosaccharomyces japonicus lig4 Disturbs Several Cellular Processes and Leads to a Pleiotropic Phenotype / Lajos Acs-Szabo, Laszlo Attila Papp, Szonja Takacs, Ida Miklos
Dátum:2023
ISSN:2309-608X
Megjegyzések:Gene targeting is a commonly used method to reveal the function of genes. Although it is an attractive tool for molecular studies, it can frequently be a challenge because its efficiency can be low and it requires the screening of a large number of transformants. Generally, these problems originate from the elevated level of ectopic integration caused by non-homologous DNA end joining (NHEJ). To eliminate this problem, NHEJ-related genes are frequently deleted or disrupted. Although these manipulations can improve gene targeting, the phenotype of the mutant strains raised the question of whether mutations have side effects. The aim of this study was to disrupt the lig4 gene in the dimorphic fission yeast, S. japonicus, and investigate the phenotypic changes of the mutant strain. The mutant cells have shown various phenotypic changes, such as increased sporulation on complete medium, decreased hyphal growth, faster chronological aging, and higher sensitivity to heat shock, UV light, and caffeine. In addition, higher flocculation capacity has been observed, especially at lower sugar concentrations. These changes were supported by transcriptional profiling. Many genes belonging to metabolic and transport processes, cell division, or signaling had altered mRNA levels compared to the control strain. Although the disruption improved the gene targeting, we assume that the lig4 inactivation can cause unexpected physiological side effects, and we have to be very careful with the manipulations of the NHEJ-related genes. To reveal the exact mechanisms behind these changes, further investigations are required.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Megjelenés:Journal of Fungi. - 9 : 5 (2023), p. 1-16. -
További szerzők:Papp László Attila (1986-) (genetikus) Takács Szonja Miklós Ida (1962-) (biológus)
Pályázati támogatás:OTKA-106172
OTKA
EFOP-3.6.1-16-2016-00022
EFOP
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2.

001-es BibID:BIBFORM097369
035-os BibID:(cikkazonosító)864 (WoS)000715414500001 (Scopus)85118167505
Első szerző:Ács-Szabó Lajos (biológus)
Cím:Genome Comparisons of the Fission Yeasts Reveal Ancient Collinear Loci Maintained by Natural Selection / Acs-Szabo Lajos, Papp Laszlo Attila, Sipiczki Matthias, Miklos Ida
Dátum:2021
ISSN:2309-608X
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Megjelenés:Journal of Fungi. - 7 : 10 (2021), p. 1-26. -
További szerzők:Papp László Attila (1986-) (genetikus) Sipiczki Mátyás (1948-) (biológus) Miklós Ida (1962-) (biológus)
Pályázati támogatás:EFOP-3.6.1-16-2016-00022
EFOP
20428-3/2018
FIKP
TKP2020-IKA-04
Egyéb
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3.

001-es BibID:BIBFORM120552
Első szerző:Bodnár Veronika
Cím:Aspergillus nidulans gfdB, Encoding the Hyperosmotic Stress Protein Glycerol-3-phosphate Dehydrogenase, Disrupts Osmoadaptation in Aspergillus wentii / Veronika Bodnár, Károly Antal, Ronald P. de Vries, István Pócsi,Tamás Emri
Dátum:2024
ISSN:2309-608X
Megjegyzések:The genome of the osmophilic Aspergillus wentii, unlike that of the osmotolerant Aspergillus nidulans, contains only the gfdA, but not the gfdB, glycerol 3-phosphate dehydrogenase gene. Here, we studied transcriptomic changes of A. nidulans (reference strain and ?gfdB gene deletion mutant) and A. wentii (reference strain and An-gfdB expressing mutant) elicited by high osmolarity. A. nidulans showed a canonic hyperosmotic stress response characterized by the upregulation of the trehalose and glycerol metabolism genes (including gfdB), as well as the genes of the high-osmolarity glycerol (HOG) map kinase pathway. The deletion of gfdB caused only negligible alterations in the transcriptome, suggesting that the glycerol metabolism was flexible enough to compensate for the missing GfdB activity in this species. A. wentii responded differently to increased osmolarity than did A. nidulans, e.g., the bulk upregulation of the glycerol and trehalose metabolism genes, along with the HOG pathway genes, was not detected. The expression of An-gfdB in A. wentii did not abolish osmophily, but it reduced growth and caused much bigger alterations in the transcriptome than did the missing gfdB gene in A. nidulans. Flexible glycerol metabolism and hence, two differently regulated gfd genes, may be more beneficial for osmotolerant (living under changing osmolarity) than for osmophilic (living under constantly high osmolarity) species.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Aspergillus nidulans
Aspergillus wentii
glycerol-3-phosphate dehydrogenase
hyperosmotic stress
RNA sequencing
Megjelenés:Journal of Fungi. - 10 : 4 (2024), p. 1-23. -
További szerzők:Antal Károly (1956-) (kémikus) De Vries, Ronald P. Pócsi István (1961-) (vegyész) Emri Tamás (1969-) (biológus)
Pályázati támogatás:NN125671
Egyéb
K131767
Egyéb
TKP2021-EGA-20
Egyéb
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4.

001-es BibID:BIBFORM105430
035-os BibID:(cikkazonosító)1215 (WoS)000895364600001 (Scopus)85149568475
Első szerző:Borrego, Jesús
Cím:Recombinant Expression in Pichia pastoris System of Three Potent Kv1.3 Channel Blockers : Vm24, Anuroctoxin, and Ts6 / Borrego Jesús, Naseem Muhammad Umair, Sehgal Al Nasar Ahmed, Panda Lipsa Rani, Shakeel Kashmala, Gaspar Attila, Nagy Cynthia, Varga Zoltan, Panyi Gyorgy
Dátum:2022
ISSN:2309-608X
Megjegyzések:The Kv1.3 channel has become a therapeutic target for the treatment of various diseases. Several Kv1.3 channel blockers have been characterized from scorpion venom; however, extensive studies require amounts of toxin that cannot be readily obtained directly from venoms. The Pichia pastoris expression system provides a cost-effective approach to overcoming the limitations of chemical synthesis and E. coli recombinant expression. In this work, we developed an efficient system for the production of three potent Kv1.3 channel blockers from different scorpion venoms: Vm24, AnTx, and Ts6. Using the Pichia system, these toxins could be obtained in sufficient quantities (Vm24 1.6 mg/L, AnTx 46 mg/L, and Ts6 7.5 mg/L) to characterize their biological activity. A comparison was made between the activity of tagged and untagged recombinant peptides. Tagged Vm24 and untagged AnTx are nearly equivalent to native toxins in blocking Kv1.3 (Kd = 4.4 pM and Kd = 0.72 nM, respectively), whereas untagged Ts6 exhibits a 53-fold increase in Kd (Kd = 29.1 nM) as compared to the native peptide. The approach described here provides a method that can be optimized for toxin production to develop more selective and effective Kv1.3 blockers with therapeutic potential.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Pichia pastoris
recombinant peptides
scorpion venoms
Kv1.3 blockers
Megjelenés:Journal of Fungi. - 8 : 11 (2022), p. 1-15. -
További szerzők:Naseem, Muhammad Umair (1993-) (biofizikus, molekuláris biológus) Sehgal, Al Nasar Ahmed Panda, Lipsa Rani Shakeel, Kashmala Gáspár Attila (1970-) (vegyész, kémikus) Nagy Cynthia (1994-) Varga Zoltán (1969-) (biofizikus, szakfordító) Panyi György (1966-) (biofizikus)
Pályázati támogatás:K143071
OTKA
K127931
OTKA
Stipendium Hungaricum Scholarship by the Tempus Public Foundation
Egyéb
Richter Gedeon Talentum Foundation
Egyéb
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5.

001-es BibID:BIBFORM119614
035-os BibID:(cikkazonosító)221
Első szerző:Emri Tamás (biológus)
Cím:The Oxidative Stress Response Highly Depends on Glucose and Iron Availability in Aspergillus fumigatus / Tamás Emri, Károly Antal, Kinga Varga, Barnabás Csaba Gila, István Pócsi
Dátum:2024
ISSN:2309-608X
Megjegyzések:Pathogens have to cope with oxidative, iron- and carbon(glucose)-limitation stresses in the human body. To understand how combined iron-carbon limitation alters oxidative stress responses, Aspergillus fumigatus was cultured in glucose-peptone or peptone containing media supplemented or not with deferiprone as an iron chelator. Changes in the transcriptome in these cultures were recorded after H2O2 treatment. Responses to oxidative stress were highly dependent on the availability of glucose and iron. Out of the 16 stress responsive antioxidative enzyme genes, only the cat2 catalase-peroxidase gene was upregulated in more than two culturing conditions. The transcriptional responses observed in iron metabolism also varied substantially in these cultures. Only extracellular siderophore production appeared important regardless of culturing conditions in oxidative stress protection, while the enhanced synthesis of Fe-S cluster proteins seemed to be crucial for oxidative stress treated iron-limited and fast growing (glucose rich) cultures. Although pathogens and host cells live together in the same place, their culturing conditions (e.g., iron availability or occurrence of oxidative stress) can be different. Therefore, inhibition of a universally important biochemical process, like Fe-S cluster assembly, may selectively inhibit the pathogen growth in vivo and represent a potential target for antifungal therapy.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Aspergillus fumigatus
carbon limitation
deferiprone
iron homeostasis
oxidative stress
RNA sequencing
Megjelenés:Journal of Fungi. - 10 : 3 (2024), p.1-18. -
További szerzők:Antal Károly Varga Kinga Gila Csaba Barnabás (1994-) (biotechnológus, biomérnök) Pócsi István (1961-) (vegyész)
Pályázati támogatás:K 131767
Egyéb
TKP2021-EGA-20
Egyéb
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6.

001-es BibID:BIBFORM105605
035-os BibID:(cikkazonosító)1226 (WoS)000912417100001 (Scopus)85149505856
Első szerző:Emri Tamás (biológus)
Cím:Stress Responses Elicited by Glucose Withdrawal in Aspergillus fumigatus / Tamás Emri, Károly Antal, Barnabás Gila, Andrea P. Jónás, István Pócsi
Dátum:2022
ISSN:2309-608X
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
aspergillus
fumigatus
stress
Megjelenés:Journal of Fungi. - 8 : 11 (2022), p. 1-19. -
További szerzők:Antal Károly Gila Csaba Barnabás (1994-) (biotechnológus, biomérnök) Jónás Andrea P. Pócsi István (1961-) (vegyész)
Pályázati támogatás:EFOP-3.6.1-16-2016-00022
EFOP
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7.

001-es BibID:BIBFORM093928
035-os BibID:(cikkazonosító)341 (WoS)000654309100001 (Scopus)85105797365
Első szerző:Ezzat, Gaballa Kassem Ahmed (agrár)
Cím:Temporal Patterns and Inter-Correlations among Physical and Antioxidant Attributes and Enzyme Activities of Apricot Fruit Inoculated with Monilinia laxa under Salicylic Acid and Methyl Jasmonate Treatments under Shelf-Life Conditions / Ahmed Ezzat, Szilárd Szabó, Zoltán Szabó, Attila Hegedűs, Dorina Berényi, Imre J. Holb
Dátum:2021
ISSN:2309-608X
Megjegyzések:Monilinia laxa causes serious postharvest damage on apricot fruits under shelf-life storage conditions. Plant elicitors of methyl jasmonate (MeJA) and salicylic acid (SA) can reduce this damage, and their research can explain the background of the plant defense physiological processes in M. laxa-infected fruits. The aims of this study were: (i) to evaluate the effect of various concentrations of MeJA and SA on brown rot incidence (BRI) and lesion diameter (LD) of apricot fruits; (ii) to measure the temporal patterns for the effect of 0.4 mmol L?1 MeJA and 2 mmol L?1 SA treatments on BRI, LD and seven fruit measures (fruit firmness (FF), lignin content (LC), total soluble phenol content (TSPC), total antioxidant capacity (TAC) and enzyme activities of PAL, POD and SOD) in treatments of M. laxa-inoculated versus (vs.) non-inoculated fruits over an eight-day shelf-life storage period; and (iii) to determine inter-correlations among the seven fruit measures for MeJA and SA treatments. Both MeJA and SA significantly reduced BRI and LD. LC, FF, TAC, TSPC, as well as SOD and PAL activities in the MeJA and SA treatments were higher than the water-treated control in most assessment days and both inoculation treatments. In both inoculation treatments, the activity of POD in the SA-treated fruits was higher than MeJA-treated and control fruits at all dates. In MeJA vs. SA and inoculated vs. non-inoculated treatments, six variable pairs (FF vs. TSPC, FF vs. TAC, TAC vs. PAL, PAL vs. POD, PAL vs. SOD, and POD vs. SOD) showed significant inter-correlation values. Principal component analyses explained 96% and 93% of the total variance for inoculated and non-inoculated treatments, respectively. In inoculated treatments, both PC1 and PC2 explained 41% of the total variance and correlated with FF, TSPC and TAC and with PAL, SOD and POD, respectively. In non-inoculated treatments, PC1 and PC2 explained 49% and 44% of the total variance and correlated with LC, PAL, POD and SOD and with FF, TSPC and TAC, respectively. It can be concluded that MeJA and SA are useful in the practice to enhance the plant defense system against brown rot by reducing fungal growth and by improving physical and antioxidant attributes (FF, LC, TAC and TSPC) and the activity of defense-related enzymes (PAL, POD and SOD) in apricot fruits during shelf-life storage conditions.
Tárgyszavak:Agrártudományok Növénytermesztési és kertészeti tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
brown rot
lesion diameter
lignin
fruit firmness
phenol content
antioxidant capacity
defense related enzymes
Pearson correlation
regression analyses
PCA
Megjelenés:Journal of Fungi. - 7 : 5 (2021), p. 1-24. -
További szerzők:Szabó Szilárd (1974-) (geográfus) Szabó Zoltán (1960-) (kertészmérnök) Hegedűs Attila Berényi Dorina Holb Imre (1973-) (agrármérnök)
Pályázati támogatás:K 131478
OTKA
TÁMOP-4.2.4.A/2-11/1-2012-0001
TÁMOP
TKP2020-NKA-04
Egyéb
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Intézményi repozitóriumban (DEA) tárolt változat
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8.

001-es BibID:BIBFORM108068
035-os BibID:(cikkazonosító)600 (WOS)000818347500001 (Scopus)85131883040
Első szerző:Farkas Edit
Cím:Distribution Types of Lichens in Hungary That Indicate Changing Environmental Conditions / Edit Farkas, Nóra Varga, Katalin Veres, Gábor Matus, Mónika Sinigla, László Lőkös
Dátum:2022
ISSN:2309-608X
Megjegyzések:Distribution data originating from earlier herbarium collections and recent biodiversity records form the basis of distribution analyses in lichen species with different ecological requirements, where the records allowed comparisons or showed clear trends. As the occurrences of lichens are strongly correlated to background environmental conditions (e.g., air pollution, global warming), confirmed by Wirth's ecological indicator values, the analysis of distribution types has a great value for bioindication and the establishment of current and future climatic and pollution situations. Five distribution types were introduced-presented by characteristic examples (13)-according to lichen distribution maps prepared in different periods of time (representing changing environmental conditions): (1) species of decreasing occurrences by time (e.g., Lobaria pulmonaria, Menegazzia terebrata, suboceanic, acidic pollution sensitive species), (2) species with no or few former records but with increasing occurrences in recent decades (e.g., Flavoparmelia soredians, Hyperphyscia adglutinata, Solenopsora candicans, sub-Mediterranean species), (3) species with increasing and then (from c. 2000) decreasing occurrences (e.g., Scoliciosporum chlorococcum, Straminella conizaeoides, acidofrequent species), (4) species with widely increasing occurrences in recent decades (e.g., Physcia aipolioides, Piccolia ochrophora, Xanthoria parietina, nitrofrequent species), and (5) species with rapidly increasing occurrences (e.g., Absconditella lignicola, Coenogonium pineti, Evernia divaricata, rapidly spreading species). The proposed distribution types of lichen species may be applied to wider regions (the European or the global level).
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
acidofrequent
air pollution bioindication
biodiversity
climate
environmental changes
land use
nitrofrequent
rapidly spreading
substrate
time scale
Megjelenés:Journal of Fungi. - 8 : 6 (2022), p. 1-17. -
További szerzők:Varga Nóra Veres Katalin (biológus) Matus Gábor (1968-) (botanikus) Sinigla Mónika Lőkös László
Pályázati támogatás:NKFI K 124341
Egyéb
EFOP 3.4.3-16-2016-00021
EFOP
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Intézményi repozitóriumban (DEA) tárolt változat
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9.

001-es BibID:BIBFORM101910
035-os BibID:(cikkazonosító)397 (WoS)000787051700001 (Scopus)85129122957
Első szerző:Fekete Erzsébet (biotechnológus, egyetemi tanár)
Cím:Unique and Repeated Stwintrons (Spliceosomal Twin Introns) in the Hypoxylaceae / Erzsébet Fekete, Fruzsina Pénzes, Norbert Ág, Viktória Ág-Rácz, Erzsébet Sándor, Claudio Scazzocchio, Michel Flipphi, Levente Karaffa
Dátum:2022
ISSN:2309-608X
Megjegyzések:Introns are usually non-coding sequences interrupting open reading frames in pre-mRNAs [D1,2]. Stwintrons are nested spliceosomal introns, where an internal intron splits a second donor sequence into two consecutive splicing reactions leading to mature mRNA. In Hypoxylon sp. CO27-5, 36 highly sequence-similar [D1,2] stwintrons are extant (sister stwintrons). An additional 81 [D1,2] sequence-unrelated stwintrons are described here. Most of them are located at conserved gene positions rooted deep in the Hypoxylaceae. Absence of exonic sequence bias at the exon?stwintron junctions and a very similar phase distribution were noted for both groups. The presence of an underlying sequence symmetry in all 117 stwintrons was striking. This symmetry, more pronounced near the termini of most of the full-length sister stwintrons, may lead to a secondary structure that brings into close proximity the most distal splice sites, the donor of the internal and the acceptor of the external intron. The Hypoxylon stwintrons were overwhelmingly excised by consecutive splicing reactions precisely removing the whole intervening sequence, whereas one excision involving the distal splice sites led to a frameshift. Alternative (mis)splicing took place for both sister and uniquely occurring stwintrons. The extraordinary symmetry of the sister stwintrons thus seems dispensable for the infrequent, direct utilisation of the distal splice sites.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
spliceosomal introns
splicing; stwintrons
comparative genomics
stwintron dissemination
stwintron propagation
internal symmetry
single-stranded RNA secondary structure prediction
Hypoxylon sp. CO27-5
Hypoxylaceae
Megjelenés:Journal of Fungi. - 8 : 4 (2022), p. 1-23. -
További szerzők:Pénzes Fruzsina (1995-) (okleveles biomérnök) Ág Norbert (1987-) (okleveles biomérnök) Ág-Rácz Viktória (1991-) (mikrobiológus) Karaffa Erzsébet Mónika (1972-) (mikrobiológus, egyetemi tanár) Scazzocchio, Claudio Flipphi, Michel (1964-) Karaffa Levente (1971-) (biotechnológus, egyetemi tanár)
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Intézményi repozitóriumban (DEA) tárolt változat
Borító:

10.

001-es BibID:BIBFORM096118
035-os BibID:(cikkazonosító)710 (WoS)000699660000001 (Scopus)85114371224
Első szerző:Fekete Erzsébet (biotechnológus, egyetemi tanár)
Cím:Internally Symmetrical Stwintrons and Related Canonical Introns in Hypoxylaceae Species / Erzsébet Fekete, Fruzsina Pénzes, Norbert Ág, Claudio Scazzocchio, Michel Flipphi, Levente Karaffa
Dátum:2021
ISSN:2309-608X
Megjegyzések:Spliceosomal introns are pervasive in eukaryotes. Intron gains and losses have occurred throughout evolution, but the origin of new introns is unclear. Stwintrons are complex intervening sequences where one of the sequence elements (5·?donor, lariat branch point element or 3·?acceptor) necessary for excision of a U2 intron (external intron) is itself interrupted by a second (internal) U2 intron. In Hypoxylaceae, a family of endophytic fungi, we uncovered scores of donor?disrupted stwintrons with striking sequence similarity among themselves and also with canonical introns. Intron-exon structure comparisons suggest that these stwintrons have proliferated within diverging taxa but also give rise to proliferating canonical introns in some genomes. The proliferated (stw)introns have integrated seamlessly at novel gene positions. The recently proliferated (stw)introns appear to originate from a conserved ancestral stwintron characterised by terminal inverted repeats (45-55 nucleotides), a highly symmetrical structure that may allow the formation of a double?stranded intron RNA molecule. No short tandem duplications flank the putatively inserted intervening sequences, which excludes a DNA transposition?based mechanism of proliferation. It is tempting to suggest that this highly symmetrical structure may have a role in intron proliferation by (an)other mechanism(s).
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Megjelenés:Journal of Fungi. - 7 (2021), p. 1-21. -
További szerzők:Pénzes Fruzsina (1995-) (okleveles biomérnök) Ág Norbert (1987-) (okleveles biomérnök) Scazzocchio, Claudio Flipphi, Michel (1964-) Karaffa Levente (1971-) (biotechnológus, egyetemi tanár)
Pályázati támogatás:ÚNKP-20-3-I-DE-40
Egyéb
ÚNKP-20-5-DE-13
Egyéb
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Intézményi repozitóriumban (DEA) tárolt változat
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11.

001-es BibID:BIBFORM117383
035-os BibID:(cikkazonosító)1195 (WoS)001132751600001 (Scopus)85180472702
Első szerző:Flipphi, Michel
Cím:Generation, Transfer, and Loss of Alternative Oxidase Paralogues in the Aspergillaceae Family / Michel Flipphi, Alexandra Márton, Vivien Bíró, Norbert Ág, Erzsébet Sándor, Erzsébet Fekete, Levente Karaffa
Dátum:2023
ISSN:2309-608X
Megjegyzések:Abstract: Alternative oxidase (Aox) is a terminal oxidase operating in branched electron transport. The activity correlates positively with overflow metabolisms in certain Aspergilli, converting intracellular glucose by the shortest possible path into organic acids, like citrate or itaconate. Aox is nearly ubiquitous in fungi, but aox gene multiplicity is rare. Nevertheless, within the family of the Aspergillaceae and among its various species of industrial relevance?Aspergillus niger, A. oryzae, A. terreus, Penicillium rubens?paralogous aox genes coexist. Paralogous genes generally arise from duplication and are inherited vertically. Here, we provide evidence of four independent duplication events along the lineage that resulted in aox paralogues (aoxB) in contemporary Aspergillus and Penicillium taxa. In some species, three aox genes are co-expressed. The origin of the A. niger paralogue is different than that of the A. terreus paralogue, but all paralogous clades ultimately arise from ubiquitous aoxA parent genes. We found different patterns of uncorrelated gene losses reflected in the Aspergillus pedigree, albeit the original aoxA orthologues persist everywhere and are never replaced. The loss of acquired paralogues co-determines the contemporary aox gene content of individual species. In Aspergillus calidoustus, the two more ancient paralogues have, in effect, been replaced by two aoxB genes of distinct origins.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Aspergillus
Penicillium
alternative oxidase
aox gene multiplicity
gene duplication
paralogous genes
gene loss
Megjelenés:Journal of Fungi. - 9 : 12 (2023), p. 1-18. -
További szerzők:Márton Alexandra (1994-) (mikrobiológus) Bíró Vivien (1994-)c(mikrobiológus) Ág Norbert (1987-) (okleveles biomérnök) Karaffa Erzsébet Mónika (1972-) (mikrobiológus, egyetemi tanár) Fekete Erzsébet (1975-) (biotechnológus, egyetemi tanár) Karaffa Levente (1971-) (biotechnológus, egyetemi tanár)
Pályázati támogatás:ÚNKP-23-3-II-DE-359
Egyéb
ÚNKP-23-4-IDE-362
Egyéb
Internet cím:Szerző által megadott URL
DOI
Intézményi repozitóriumban (DEA) tárolt változat
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12.

001-es BibID:BIBFORM111705
035-os BibID:(cikkazonosító)570 (Scopus)85160535117 (WoS)000997803000001
Első szerző:Flipphi, Michel
Cím:Mutations in the Second Alternative Oxidase Gene: A New Approach to Group Aspergillus niger Strains / Michel Flipphi, Alexandra Márton, Vivien Bíró, Norbert Ág, Erzsébet Sándor, Erzsébet Fekete, Levente Karaffa
Dátum:2023
ISSN:2309-608X
Megjegyzések:Alternative oxidase is a terminal oxidase in the branched mitochondrial electron transport chain of most fungi including Aspergillus niger (subgenus Circumdati, section Nigri). A second, paralogous aox gene (aoxB) is extant in some A. niger isolates but also present in two divergent species of the subgenus Nidulantes A. calidoustus and A. implicatus?as well as in Penicillium swiecickii. Black aspergilli are cosmopolitan opportunistic fungi that can cause diverse mycoses and acute aspergillosis in immunocompromised individuals. Amongst the approximately 75 genome-sequenced A. niger strains, aoxB features considerable sequence variation. Five mutations were identified that rationally affect transcription or function or terminally modify the gene product. One mutant allele that occurs in CBS 513.88 and A. niger neotype strain CBS 554.65 involves a chromosomal deletion that removes exon 1 and intron 1 from aoxB. Another aoxB allele results from retrotransposon integration. Three other alleles result from point mutations: a missense mutation of the start codon, a frameshift, and a nonsense mutation. A. niger strain ATCC 1015 has a full-length aoxB gene. The A. niger sensu stricto complex can thus be subdivided into six taxa according to extant aoxB allele, which may facilitate rapid and accurate identification of individual species
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
ubiquinol:oxygen oxidoreductase encoding gene
Aspergillus niger sensu stricto complex
alleles
chromosomal deletion
retrotransposon insertion
point mutation
frameshift mutation
nonsense mutation
molecular taxonomy
Megjelenés:Journal of Fungi. - 9 : 5 (2023), p. 1-17. -
További szerzők:Márton Alexandra (1994-) (mikrobiológus) Bíró Vivien (1994-)c(mikrobiológus) Ág Norbert (1987-) (okleveles biomérnök) Karaffa Erzsébet Mónika (1972-) (mikrobiológus, egyetemi tanár) Fekete Erzsébet (1975-) (biotechnológus, egyetemi tanár) Karaffa Levente (1971-) (biotechnológus, egyetemi tanár)
Internet cím:Szerző által megadott URL
DOI
Intézményi repozitóriumban (DEA) tárolt változat
Borító:
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