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1.

001-es BibID:BIBFORM110207
035-os BibID:(cikkazonosító)852 (Scopus)85156240803 (WoS)000978247400001
Első szerző:Csoma Hajnalka (biológus, szőlész-borász)
Cím:Characterization of Zygosaccharomyces lentus Yeast in Hungarian Botrytized Wines / Hajnalka Csoma, Lajos Acs-Szabo, László Attila Papp, Zoltán Kállai, Ida Miklós, Matthias Sipiczki
Dátum:2023
ISSN:2076-2607
Megjegyzések:Tokaj botrytized sweet wines are traditionally aged for several years in wood barrels or bottles. As they have significant residual sugar content, they are exposed to microbial contamination during ageing. Osmotolerant wine-spoilage yeasts are most commonly found in the Tokaj winegrowing region in the species Starmerella spp. and Zygosaccharomyces spp. For the first time, Z. lentus yeasts were isolated from post-fermented botrytized wines. Our physiological studies confirmed that these yeast strains are osmotolerant, with high sulphur tolerance and 8% v/v alcohol tolerance, and that they grow well at cellar temperature in acidic conditions. Low β-glucosidase and sulphite reductase activities were observed, whereas protease, cellulase, and α-arabinofuranosidase extracellular enzyme activities were not detected. Molecular biology analyses carried out by RFLP analysis of mtDNA revealed no remarkable differences between strains, while microsatellite-primed-PCR fingerprinting of the (GTG)5 microsatellite and examination of chromosomal pattern revealed considerable diversity. The fermentative vigour of the tested Z. lentus strains was found to be significantly lower compared to the control Saccharomyces cerevisiae (Lalvin EC1118). It can be concluded that Z. lentus is a potential spoilage yeast in oenology which may be responsible for the initiation of secondary fermentation of wines during ageing.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Zygosaccharomyces
spoilage yeast
botrytized wine
post-fermentation
Megjelenés:Microorganisms. - 11 : 4 (2023), p. 1-17. -
További szerzők:Ács-Szabó Lajos (1985-) (biológus) Papp László Attila (1986-) (genetikus) Kállai Zoltán (1986-) (biológus) Miklós Ida (1962-) (biológus) Sipiczki Mátyás (1948-) (biológus)
Pályázati támogatás:K-124417
NKFIH
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2.

001-es BibID:BIBFORM096892
035-os BibID:(cikkazonosító)19 (WoS)000610590500001 (Scopus)85098665221
Első szerző:Csoma Hajnalka (biológus, szőlész-borász)
Cím:Vinification without Saccharomyces: Interacting Osmotolerant and "Spoilage" Yeast Communities in Fermenting and Ageing Botrytised High-Sugar Wines (Tokaj Essence) / Csoma Hajnalka, Kállai Zoltán, Antunovics Zsuzsa, Czentye Kinga, Sipiczki Matthias
Dátum:2021
ISSN:2076-2607
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
yeast
fermentation
Megjelenés:Microorganisms. - 9 : 1 (2021), p. 1-28. -
További szerzők:Kállai Zoltán (1986-) (biológus) Antunovics Zsuzsa (1978-) (molekuláris biológus, genetikus) Czentye Kinga Sipiczki Mátyás (1948-) (biológus)
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3.

001-es BibID:BIBFORM095574
035-os BibID:(cikkazonosító)1433 (WoS)000676396300001
Első szerző:Emri Tamás (biológus)
Cím:AtfA-Independent Adaptation to the Toxic Heavy Metal Cadmium in Aspergillus nidulans / Tamás Emri, Barnabás Gila, Károly Antal, Fanni Fekete, Heungyun Moon, Jae-Hyuk Yu, István Pócsi
Dátum:2021
ISSN:2076-2607
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Megjelenés:Microorganisms. - 9 : 7 (2021), p. 1-23. -
További szerzők:Gila Barnabás (1994-) (biotechnológus, biomérnök) Antal Károly (1956-) (kémikus) Fekete Fanni Moon, Heungyun Yu Jae-Hyuk Pócsi István (1961-) (vegyész)
Pályázati támogatás:EFOP-3.6.1-16-2016-00022
EFOP
ÚNKP-20-3
Egyéb
K112181
Egyéb
K119494
Egyéb
NN125671
Egyéb
K131767
Egyéb
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4.

001-es BibID:BIBFORM109570
035-os BibID:(cikkazonosító)586 (Scopus)85151426418 (WoS)000956870300001
Első szerző:Juhász Gabriella Petra (biologia tanár)
Cím:Microcystin-LR, a Cyanobacterial Toxin, Induces Changes in the Organization of Membrane Compartments in Arabidopsis / Gabriella Petra Juhász, Sándor Kéki, Anita Dékány-Adamoczky, Csongor Freytag, Gábor Vasas, Csaba Máthé, Tamás Garda
Dátum:2023
ISSN:2076-2607
Megjegyzések:To evaluate the effects of the cyanobacterial toxin microcystin-LR (MCY-LR, a protein phosphatase inhibitor) and diquat (DQ, an oxidative stress inducer) on the organization of tonoplast, the effect of MCY-LR on plastid stromule formation and on mitochondria was investigated in wildtype Arabidopsis. Tonoplast was also studied in PP2A catalytic (c3c4) and regulatory subunit mutants (fass-5 and fass-15). These novel studies were performed by CLSM microscopy. MCY-LR is produced during cyanobacterial blooms. The organization of tonoplast of PP2A mutants of Arabidopsis is much more sensitive to MCY-LR and DQ treatments than that of wild type. In c3c4, fass-5 and fass-15, control and treated plants showed increased vacuole fragmentation that was the strongest when the fass-5 mutant was treated with MCY-LR. It is assumed that both PP2A/C and B" subunits play an important role in normal formation and function of the tonoplast. In wild-type plants, MCY-LR affects mitochondria. Under the influence of MCY-LR, small, round-shaped mitochondria appeared, while long/fused mitochondria were typical in control plants. Presumably, MCY-LR either inhibits the fusion of mitochondria or induces fission. Consequently, PP2A also plays an important role in the fusion of mitochondria. MCY-LR also increased the frequency of stromules appearing on chloroplasts after 1 h treatments. Along the stromules, signals can be transported between plastids and endoplasmic reticulum. It is probable that they promote a faster response to stress.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Megjelenés:Microorganisms. - 11 : 3 (2023), p. 1-13. -
További szerzők:Kéki Sándor (1964-) (polimer kémikus) Dékány-Adamoczky Anita (1985-) (anyagmérnök) Freytag Csongor (1993-) (biológus) Vasas Gábor (1975-) (biológus-vegyész) Máthé Csaba (1966-) (biológus) Garda Tamás (1990-) (biológus)
Pályázati támogatás:OTKA-120638
OTKA
OTKA-132685
OTKA
TKP2021-EGA-20
Egyéb
ÚNKP-22-4-I-DE-11
Egyéb
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5.

001-es BibID:BIBFORM094999
035-os BibID:(WoS)000666456900001 (Scopus)85107497751
Első szerző:Karaffa Levente (biotechnológus, egyetemi tanár)
Cím:The role of metal ions in fungal organic acid accumulation / Levente Karaffa, Erzsébet Fekete, Christian P. Kubicek
Dátum:2021
ISSN:2076-2607
Megjegyzések:Organic acid accumulation is probably the best-known example of primary metabolic overflow. Both bacteria and fungi are capable of producing various organic acids in large amounts under certain conditions, but in terms of productivity-and consequently, of commercial importance-fungal platforms are unparalleled. For high product yield, chemical composition of the growth medium is crucial in providing the necessary conditions, of which the concentrations of four of the first-row transition metal elements, manganese (Mn2+), iron (Fe2+), copper (Cu2+) and zinc (Zn2+) stand out. In this paper we critically review the biological roles of these ions, the possible biochemical and physiological consequences of their influence on the accumulation of the most important mono-, di- and tricarboxylic as well as sugar acids by fungi, and the metal ion-related aspects of submerged organic acid fermentations, including the necessary instrumental analytics. Since producing conditions are associated with a cell physiology that differs strongly to what is observed under "standard" growth conditions, here we consider papers and patents only in which organic acid accumulation levels achieved at least 60% of the theoretical maximum yield, and the actual trace metal ion concentrations were verified.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
manganese(II) ions
acopper(II) ions
iron(II) ions
zinc(II) ion
organic acid fermentations
filamentous fungi
Megjelenés:Microorganisms. - 9 : 6 (2021), p. 1-16. -
További szerzők:Fekete Erzsébet (1975-) (biotechnológus, egyetemi tanár) Kubicek, Christian P.
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6.

001-es BibID:BIBFORM104434
035-os BibID:(cikkazonosító)2295 (WOS)000723780000001 (Scopus)85118337433
Első szerző:Nĕmcová, Andrea
Cím:Use of Waste Substrates for the Lipid Production by Yeasts of the Genus Metschnikowia - Screening Study / Andrea Nĕmcová, Martin Szotkowski, Ota Samek, Linda Cagáňová, Matthias Sipiczki, Ivana Márová
Dátum:2021
ISSN:2076-2607
Megjegyzések:Oleogenic yeasts are characterized by the ability to accumulate increased amounts of lipids under certain conditions. These microbial lipids differ in their fatty acid composition, which allows them to be widely used in the biotechnology industry. The interest of biotechnologists is closely linked to the rising prices of fossil fuels in recent years. Their negative environmental impact is caused by significantly increased demand for biodiesel. The composition of microbial lipids is very similar to vegetable oils, which provides great potential for use in the production of biodiesel. In addition, some oleogenic microorganisms are capable of producing lipids with a high proportion of unsaturated fatty acids. The presented paper's main aim was to study the production of lipids and lipid substances by yeasts of the genus Metschnikowia, to cultivate crude waste animal fat to study its utilization by yeasts, and to apply the idea of circular economy in the biotechnology of Metschnikowia yeasts. The work focuses on the influence of various stress factors in the cultivation process, such as reduced temperature or nutritional stress through the use of various waste substrates, together with manipulating the ratio of carbon and nitrogen sources in the medium. Yeast production properties were monitored by several instrumental techniques, including gas chromatography and Raman spectroscopy. The amount of lipids and in particular the fatty acid composition varied depending on the strains studied and the culture conditions used. The ability of yeast to produce significant amounts of unsaturated fatty acids was also demonstrated in the work. The most suitable substrate for lipid production was a medium containing glycerol, where the amount of accumulated lipids in the yeast M. pulcherrima 1232 was up to 36%. In our work, the crude animal fat was used for the production of high-value lipids, which to the best of our knowledge is a new result. Moreover, quantitative screening of lipase enzyme activity cultivated on animal fat substrate on selected yeasts of the genus Metschnikowia was performed. We found that for the yeast utilizing glycerol, animal fat seems to be an excellent source of carbon. Therefore, the yeast conversion of crude processed animal fat to value-added products is a valuable process for the biotechnology and food industry.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
yeasts
Metschnikowia
animal fat
lipids
stress factors
Raman spectroscopy
Megjelenés:Microorganisms. - 9 : 11 (2021), p. 1-23. -
További szerzők:Szotkowski, Martin Samek, Ota Cagáňová, Linda Sipiczki Mátyás (1948-) (biológus) Marova, Ivana
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7.

001-es BibID:BIBFORM104433
035-os BibID:(cikkazonosító)26 (WOS)000758821200001 (Scopus)85121724236
Első szerző:Sipiczki Mátyás (biológus)
Cím:Wine Yeasts 1.0 / Matthias Sipiczki
Dátum:2021
ISSN:2076-2607
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Megjelenés:Microorganisms. - 10 : 1 (2021), p. 1-4. -
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8.

001-es BibID:BIBFORM104053
035-os BibID:(cikkazonosító)1029 (WOS)00055420760000 (Scopus)85089603735
Első szerző:Sipiczki Mátyás (biológus)
Cím:Metschnikowia pulcherrima and Related Pulcherrimin-Producing Yeasts: Fuzzy Species Boundaries and Complex Antimicrobial Antagonism / Matthias Sipiczki
Dátum:2020
ISSN:2076-2607
Megjegyzések:Yeasts affiliated with theMetschnikowia pulcherrimaclade (subclade) of the large ascomycetous genusMetschnikowiafrequently turn out to produce the characteristic maroon-red pulcherrimin when tested for pigment production and prove to exert antagonistic effects on many types of microorganisms. The determination of the exact taxonomic position of the strains is hampered by the shortage of distinctive morphological and physiological properties of the species of the clade and the lack of rDNA barcode gaps. The rDNA repeats of the type strains of the species are not homogenized and are assumed to evolve by a birth-and-death mechanism combined with reticulation. The taxonomic division is further hampered by the incomplete biological (reproductive) isolation of the species: certain type strains can be hybridized and genome sequencing revealed chimeric genome structures in certain strains that might have evolved from interspecies hybrids (alloploid genome duplication). Various mechanisms have been proposed for the antimicrobial antagonism. One is related to pulcherrimin production. The diffusible precursor of pulcherrimin, the pulcherriminic acid is secreted by the cells into the environment where it forms the insoluble pulcherrimin with the ferric ions. The lack of free iron caused by the immobilization of ferric ions inhibits the growth of many microorganisms. Recent results of research into the complexity of the taxonomic division of the pulcherrimin-producingMetschnikowiayeasts and the mechanism(s) underlying their antimicrobial antagonism are discussed in this review.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Metschnikowia
taxonomy
barcodes
genome
reticulation
antagonism
pulcherrimin
iron-depletion
bioprotection
Megjelenés:Microorganisms. - 8 : 7 (2020), p. 1-19. -
Pályázati támogatás:NKFIH-K-124417
Egyéb
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9.

001-es BibID:BIBFORM078607
035-os BibID:(cikkazonosító)5 (WoS)000457124000003 (Scopus)85061664892
Első szerző:Váradi Györgyi
Cím:Structure and synthesis of antifungal disulfide β-strand proteins from filamentous fungi / Györgyi Váradi, Gábor K. Tóth, Gyula Batta
Dátum:2018
ISSN:2076-2607
Megjegyzések:The discovery and understanding of the mode of action of new antimicrobial agents is extremely urgent, since fungal infections cause 1.5 million deaths annually. Antifungal peptides and proteins represent a significant group of compounds that are able to kill pathogenic fungi. Based on phylogenetic analyses the ascomycetous, cysteine-rich antifungal proteins can be divided into three different groups: Penicillium chrysogenum antifungal protein (PAF), Neosartorya fischeri antifungal protein 2 (NFAP2) and ?bubble-proteins" (BP) produced, for example, by P. brevicompactum. They all dominantly have β-strand secondary structures that are stabilized by several disulfide bonds. The PAF group (AFP antifungal protein from Aspergillus giganteus, PAF and PAFB from P. chrysogenum, Neosartorya fischeri antifungal protein (NFAP)) is the best characterized with their common β-barrel tertiary structure. These proteins and variants can efficiently be obtained either from fungi production or by recombinant expression. However, chemical synthesis may be a complementary aid for preparing unusual modifications, e.g., the incorporation of non-coded amino acids, fluorophores, or even unnatural disulfide bonds. Synthetic variants up to ca. 6-7 kDa can also be put to good use for corroborating structure determination. A short overview of the structural peculiarities of antifungal β-strand disulfide bridged proteins will be given. Here, we describe the structural propensities of some known antifungal proteins from filamentous fungi which can also be prepared with modern synthetic chemistry methods.
Tárgyszavak:Természettudományok Kémiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
structure
antifungal protein
chemical synthesis
solid-phase peptide synthesis
native chemical ligation
disulfide bond
NFAP2
PAF
Megjelenés:Microorganisms. - 7 : 1 (2018), p. 1-10. -
További szerzők:Tóth Gábor (Szeged) Batta Gyula (1953-) (molekula-szerkezet kutató)
Pályázati támogatás:GINOP-2.3.2-15-2016-00008
GINOP
GINOP-2.3.2-15-2016-00014
GINOP
GINOP-2.3.3-15-2016-00004
GINOP
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10.

001-es BibID:BIBFORM104436
035-os BibID:(cikkazonosító)277 (WOS)000622821100001 (Scopus)85100040328
Cím:The Use of Raman Spectroscopy to Monitor Metabolic Changes in Stressed Metschnikowia sp. Yeasts / Andrea Němcová, Dominika Gonová, Ota Samek, Matthias Sipiczki, Emilia Breierová, Ivana Márová
Dátum:2021
ISSN:2076-2607
Megjegyzések:Raman spectroscopy is a universal method designed for the analysis of a wide range of physical, chemical and biological systems or various surfaces. This technique is suitable to monitor various components of cells, tissues or microorganisms. The advantages include very fast non-contact and non-destructive analysis and no or minimal need for sample treatment. The yeasts Metschnikowia can be considered as industrially usable producers of pulcherrimin or single-cell lipids, depending on cultivation conditions and external stress. In the present study, Raman spectroscopy was used as an effective tool to identify both pulcherrimin and lipids in single yeast cells. The analysis of pulcherrimin is very demanding; so far, there is no optimal procedure to analyze or identify this pigment. Based on results, the strong dependence of pulcherrimin production on the ferric ion concentration was found with the highest yield in media containing 0.1 g/L iron. Further, production of lipids in Metschnikowia cells was studied at different temperatures and C:N ratios, using Raman spectroscopy to follow fatty acids composition, under different regimes, by monitoring the iodine number. The results of Raman spectroscopy were comparable with the fatty acid analysis obtained by gas chromatography. This study therefore supported use of Raman spectroscopy for biotechnological applications as a simple tool in the identification and analysis both the pulcherrimin and microbial lipids. This method provides a quick and relatively accurate estimation of targeted metabolites with minimal sample modification and allows to monitor metabolic changes over time of cultivation.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
yeasts
Metschnikowia
lipids
pulcherrimin
Raman spectroscopy
Megjelenés:Microorganisms. - 9 : 2 (2021), p. 1-19. -
További szerzők:Nĕmcová, Andrea Gonová, Dominika Samek, Ota Sipiczki Mátyás (1948-) (biológus) Breierová, Emilia Marova, Ivana
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