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001-es BibID:	BIBFORM004678
035-os BibID:	(scopus)0035153667 (wos)000171533400007
Első szerző:	Barabás György (sejtbiológus, molekuláris genetikus)
Cím:	N-Alkane uptake and utilisation by Streptomyces strains / Barabas, G., Vargha, G., Szabo, I. M., Penyige, A., Damjanovich, S., Szollosi, J., Matko, J., Hirano, T., Matyus, A., Szabo, I.
Dátum:	2001
Megjegyzések:	Streptomyces strains isolated from the Kuwait Burgan oil field were defined as S. griseoflavus, S. parvus, and S. plicatus utilised n-hexadecane, n-octadecane (purified fractions of mineral oil), kerosene, and crude oil as sole carbon and energy sources. The strains were incubated with n-alkanes and increase of the fatty acid content with chain length equivalent to the employed n-alkanes was observed. Signal transducing GTP-binding proteins (GBPs) play an important role in n-alkane uptake in streptomycetes. Specific activators of GBPs increased the uptake of hydrocarbons. Using the hydrophobic fluorescent dye diphenylhexatrien (DPH) as a probe, it was found that the microviscosity of the hydrophobic inner region of the cellular membrane is significantly lower in hydrocarbon utilisers than in non-utilisers. This difference probably reflects differences in the fatty acid composition of the strains. When cultures were grown in n-alkane containing media, electron microscopy revealed that the hydrocarbon utilisers showed less-electron dense areas as inclusions in the cytoplasm. Soil samples inoculated with Streptomyces strains eliminated hydrocarbons much faster than those not containing these strains, serving as control. When inorganic medium was supplied with n-hexadecane-1-14C as sole carbon and energy source, radioactive CO2 was detected. Since streptomycetes have not been used until now for oil elimination, though they are known as abundant soil bacteria tolerating extreme conditions, their possible use for bioremediation of hydrocarbon contaminated soils is discussed.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk Alkanes analogs and derivatives analysis Biodegradation Cell Membrane Cell Membrane Permeability chemistry Cytoplasm Diphenylhexatriene Dyes Fatty Acids Fluorescent Dyes genetics GTP-Binding Proteins Human Hungary Hydrocarbons metabolism Microscopy physiology Streptomyces Support,Non-U.S.Gov't ultrastructure
Megjelenés:	Antonie Van Leeuwenhoek. - 79 : 3-4 (2001), p. 269-276. -
További szerzők:	Vargha György (1951-) (orvos) Szabó István M. Penyige András (1954-) (molekuláris genetikus) Damjanovich Sándor (1936-2017) (biofizikus) Szöllősi János (1953-) (biofizikus) Matkó János (1952-) (biológus) Hirano, Tadashi Mátyus Anita Szabó István
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001-es BibID:	BIBFORM025194
Első szerző:	Biró Sándor (molekuláris genetikus)
Cím:	Transcriptional and functional analysis of the gene for factor C, an extracellular signal protein involved in cytodifferentiation of Streptomyces griseus / Sándor Biró, Zsuzsanna Birkó, Gilles P. van Wezel
Dátum:	2000
Megjegyzések:	Factor C is an extracellular signal protein involved in cellular differentiation in Streptomyces griseus. Nuclease S1 mapping experiments revealed that transcription of the gene takes place from a single promoter in a developmental-stage specific manner. The latter was also confirmed by in vivo promoter probing. The sequence of its promoter suggests that the gene is not transcribed by the major sigma factor. The cloned gene expressed from its own promoter in low- and high-copy-number vectors restored normal sporulation to a bald mutant of Streptomyces griseus. Computer analysis of the amino acid sequence revealed the presence of a transmembrane localization segment with the N-terminus positioned inside the cell. These data fit well into our working model that points at an important role for factor C in the morphogenesis of Streptomyces griseus.
Tárgyszavak:	Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	Antonie Van Leeuwenhoek. - 78 : 3-4 (2000), p. 277-285. -
További szerzők:	Hádáné Birkó Zsuzsanna (1971-) (molekuláris genetikus) Wezel, Gilles P., van
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat Elektronikus változat DOI
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