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001-es BibID:BIBFORM025200
Első szerző:Fekete Erzsébet (biotechnológus, egyetemi tanár)
Cím:Regulation of formation of the intracellular β-galactosidase activity of Aspergillus nidulans / Erzsébet Fekete, Levente Karaffa, Erzsébet Sándor, Bernhard Seiboth, Sándor Biró, Attila Szentirmai, Christian P. Kubicek
Dátum:2002
Megjegyzések:The regulation of formation of the single intracellular beta-galactosidase activity of Aspergillus nidulans was investigated. beta-Galactosidase was not formed during growth on glucose or glycerol, but was rapidly induced during growth on lactose or D-galactose. L-Arabinose, and - with lower efficacy - D-xylose also induced beta-galactosidase activity. Addition of glucose to cultures growing on lactose led to a rapid decrease in beta-galactosidase activity. In contrast, in cultures growing on beta-galactose, addition of glucose decreased the activity of beta-galactosidase only slightly. Glucose inhibited the uptake of lactose, but not of D-galactose, and required the carbon catabolite repressor CreA for this. In addition, CreA also repressed the formation of basal levels of beta-galactosidase and partially interfered with the induction of beta-galactosidase by D-galaclose, L-arabinose, and D-xylose. D-Galactose phosphorylation was not necessary for beta-galactosidase induction, since induction by D-galactose occurred in an A. nidulans mutant defective in galactose kinase, and by the non-metabolizable D-galactose analogue fucose in the wild-type strain. Interestingly, a mutant in galactose-1-phosphate uridylyl transferase produced beta-galactosidase at a low, constitutive level even on glucose and glycerol and was no longer inducible by D-galactose, whereas it was still inducible by L-arabinose. We conclude that biosynthesis of the intracellular beta-galactosidase of A. nidulans is regulated by CreA,. partially repressed by galactose-1-phosphate uridylyl transferase, and induced by D-galactose and L-arabinose in independent ways.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Archives of Microbiology. - 179 : 1 (2002), p. 7-14. -
További szerzők:Karaffa Levente (1971-) (biotechnológus, egyetemi tanár) Karaffa Erzsébet Mónika (1972-) (mikrobiológus, egyetemi tanár) Seiboth, Bernhard Biró Sándor (1949-) (molekuláris genetikus) Szentirmai Attila (1930-) (mikrobiológus) Kubicek, Christian P.
Internet cím:DOI
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001-es BibID:BIBFORM057736
Első szerző:Spitzmüller Zsolt (molekuláris biológus)
Cím:[Gamma]-Glutamyl transpeptidase (GgtA) of Aspergillus nidulans is not necessary for bulk degradation of glutathione / Zsolt Spitzmüller, Nak-Jung Kwon, Melinda Szilágyi, Judit Keserű, Viktória Tóth, Jae-Hyuk Yu, István Pócsi, Tamás Emri
Dátum:2015
ISSN:0302-8933 1432-072X
Megjegyzések:Aspergillus nidulans exhibited high [Gamma]-glutamyl transpeptidase ([Gamma]GT) activity in both carbon-starved and carbon-limited cultures. Glucose repressed, but casein peptone increased [Gamma]GT production. Null mutation of creA did not influence [Gamma]GT formation, but the functional meaBwas necessary for the [Gamma]GT induction. Deletion of the AN10444 gene (ggtA) completely eliminated the [Gamma]GT activity, and the mRNA levels of ggtA showed strong correlation with the observed [Gamma]GT activities. While ggtA does not contain a canonical signal sequence, the [Gamma]GT activity was detectable both in the fermentation broth and in the hyphae. Deletion of the ggtAgene did not prevent the depletion of glutathione observed in carbon-starved and carbon-limited cultures. Addition of casein peptone to carbon-starved cultures lowered the formation of reactive species (RS). Deletion of ggtA could hinder this decrease and resulted in elevated RS formation. This effect of [Gamma]GT on redox homeostasis may explain the reduced cleistothecia formation of ?ggtA strains in surface cultures
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Archives Of Microbiology. - 197 : 2 (2015), p. 285-297. -
További szerzők:Kwon, Nak-Jung Szilágyi Melinda (1984-) (biológus) Keserű Judit (1976-) (molekuláris genetikus) Tóth Viktória (1984-) (biológus) Yu Jae-Hyuk Pócsi István (1961-) (vegyész) Emri Tamás (1969-) (biológus)
Pályázati támogatás:TÁMOP-4.2.2.A-11/1/KONV-2012-0045
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