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001-es BibID:BIBFORM060508
Első szerző:Albert Réka
Cím:Triamcinolone regulated apopto-phagocytic gene expression patterns in the clearance of dying retinal pigment epithelial cells. A key role of Mertk in the enhanced phagocytosis / Réka Albert, Endre Kristóf, Gábor Zahuczky, Mária Szatmári-Tóth, Zoltán Veréb, Brigitta Oláh, Morten C. Moe, Andrea Facskó, László Fésüs, Goran Petrovski
Dátum:2015
ISSN:0304-4165
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Megjelenés:Biochimica et Biophysica Acta (BBA). General Subjects. - 1850 : 2 (2015), p. 435-446. -
További szerzők:Kristóf Endre (1987-) (általános orvos) Zahuczky Gábor (1975-) (molekuláris biológus, biokémikus, vegyész) Szatmári-Tóth Mária (1987-) (molekuláris biológus) Veréb Zoltán (1980-) (immunológus, mikrobiológus, molekuláris biológus) Oláh Brigitta Moe, Morten C. Facskó Andrea (1953-) (szemész) Fésüs László (1947-) (orvos biokémikus) Petrovski, Goran (1975-) (orvos)
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DOI
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2.

001-es BibID:BIBFORM075129
Első szerző:Bene László (biofizikus)
Cím:Confocal microscopic dual-laser dual-polarization FRET (2polFRET) at the acceptor side for correlating rotations at different distances on the cell surface / László Bene, Matthias Gralle, László Damjanovich
Dátum:2018
ISSN:0304-4165
Megjegyzések:Relationship of donor and acceptor fluorescence anisotropies as well as efficiency of fluorescence resonanceenergy transfer (FRET) has been investigated in a confocal microscope in the context of FRET systems comprisedof donor and acceptor-labeled MHCI and MHCII receptors on the surface of Kit-225 K6 human T-cells. Themeasurements have been carried out in a 2-laser, 5-signal platform where the total donor fluorescence intensityand 2 acceptor fluorescence intensities with their anisotropies ? one at the donor's excitation wavelength, theother at the acceptor's excitation wavelength ? have been detected. This configuration enabled the determinationof FRET efficiency and correlating it with the two acceptor fluorescence anisotropies as a kind of calibration.Estimations for the FRET-enhanced donor fluorescence anisotropy, the directly excited acceptor fluorescenceanisotropy, and the fluorescence anisotropy of sensitized emission have been obtained. Procedures for determiningFRET by measuring only the total donor intensity and the acceptor intensity and its anisotropy, or twoacceptor intensities and their anisotropies have been elaborated, the errors of which have been estimated basedon the fluorescence anisotropy values obtained in the calibration with the method of flow cytometric energytransfer (FCET).The combined detection of the donor and acceptor fluorescence anisotropies enabled also the determinationof the lower and upper limits of the orientation factor for FRET (?2). An increase in range for ?2 with increasingFRET efficiency has been observed, with average ?2 values different from the dynamic random average of 2/3.These observations call for the need of ?2 determination in proximity measurements, where the donor andacceptor orientations are not predictable.An increasing range of ?2 with increasing intermolecular proximity of the MHCI and MHCII receptors hasbeen observed. This indicates that molecular flexibility in the clusters of the MHCI and MHCII receptors reduceswith increasing cluster density, i.e. a "fluidity gradient" exists in the clusters. More specifically, the local densitydependent flexibility can also be taken as a direct proof for that the association of these receptors is non-random,but mediated by some type of physical interaction, a finding as a benefit of FRET detection by polarizationspectroscopy.Two new quantities ? the quenched donor fluorescence anisotropy and a fluorescence anisotropy analogue,the "dissymmetry index" of the polarized FRET efficiency components ? have also been introduced for thecharacterization of the orientational dynamics of the excited state during FRET.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Dual-anisotropy correlations
Donor anisotropy
Acceptor anisotropy
Orientation factor (k2)-imaging
Anisotropy multiplexing
rFLIM
Megjelenés:Biochimica et Biophysica Acta (BBA). General Subjects. - 1862 : 4 (2018), p. 1050-1068. -
További szerzők:Gralle, Matthias Damjanovich László (1960-) (általános sebész)
Pályázati támogatás:TÁMOP-4.2.2.A-11/1/KONV-2012-0045
TÁMOP
Sebészet Kutatócsoport
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3.

001-es BibID:BIBFORM048515
Első szerző:Shrestha, Dilip (biológus)
Cím:CD1d favors MHC neighborhood, GM1 ganglioside proximity and low detergent sensitive membrane regions on the surface of B lymphocytes / Dilip Shrestha, Mark A. Exley, György Vereb, János Szöllősi, Attila Jenei
Dátum:2014
ISSN:0304-4165
Megjegyzések:BACKGROUND: Cluster of differentiation 1 (CD1) represents a family of proteins which is involved in lipid-based antigen presentation. Primarily, antigen presenting cells, like B cells, express CD1 proteins. Here, we examined the cell-surface distribution of CD1d, a subtype of CD1 receptors, on B lymphocytes. METHODS: Fluorescence labeling methods, including fluorescence resonance energy transfer (FRET), were employed to investigate plasma membrane features of CD1d receptors. RESULTS: High FRET efficiency was observed between CD1d and MHC I heavy chain (MHC I-HC), beta2-microglobulin (beta2m) and MHC II proteins in the plasma membrane. In addition, overexpression of CD1d reduced the expression of MHC II and increased the expression of MHC I-HC and beta2m proteins on the cell-surface. Surprisingly, beta2m dependent CD1d isoform constituted only ~15% of the total membrane CD1d proteins. Treatment of B cells with methyl-beta-cyclodextrin (MbetaCD) / simvastatin caused protein rearrangement; however, FRET demonstrated only minimal effect of these chemicals on the association between CD1d and GM1 ganglioside on cell-surface. Likewise, a modest effect was only observed in a co-culture assay between MbetaCD/simvastatin treated C1R-CD1d cells and invariant natural killer T cells on measuring secreted cytokines (IFNgamma and IL4). Furthermore, CD1d rich regions were highly sensitive to low concentration of Triton X-100. Physical proximity between CD1d, MHC and GM1 molecules was also detected in the plasma membrane. CONCLUSIONS: An intricate relationship between CD1d, MHC, and lipid species were found on the membrane of human B cells. General significance Organization of CD1d on the plasma membrane might be critical for its biological functions.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
CD1d
MHC
FRET
Rafts
Simvastatin
Megjelenés:Biochimica et Biophysica Acta (BBA). General Subjects. - 1840 : 1 (2014), p. 667-680. -
További szerzők:Exley, Mark A. (1961-) (immunológus) Vereb György (1965-) (biofizikus, orvos) Szöllősi János (1953-) (biofizikus) Jenei Attila (1966-) (biofizikus)
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