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1.

001-es BibID:BIBFORM083093
Első szerző:Almássy János (élettanász, biológus, angol-magyar szakfordító)
Cím:Investigation of BK Channel Gating using Mallotoxin / Almassy Janos, Begenisich Ted
Dátum:2011
ISSN:0006-3495
Tárgyszavak:Orvostudományok Elméleti orvostudományok idézhető absztrakt
folyóiratcikk
Megjelenés:Biophysical Journal. - 100 : 3 (2011), p. 583a. -
További szerzők:Begenisich, Ted B.
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2.

001-es BibID:BIBFORM049388
Első szerző:Almássy János (élettanász, biológus, angol-magyar szakfordító)
Cím:Inhibition of Ryr1 by different lanthanides might reveal fine details of the ion conducting pore / Janos Almassy, Zsanett Topcsiov, Anett Szabo, Istvan Jona
Dátum:2008
ISSN:0006-3495
Megjegyzések:Effect of Europium on the gating of the RyR1 was investigated using Müuller-type artificial bilayer system. Europium applied on the trans side inhibited the RyR1 channel by Kd = 4.7 ? 0.1 mM (similarly to the Gd3+ effect), with a high cooperativity, as characterized by a Hill-coefficient of N = 5.9 ? 0.9 in contrast to Gadolinium, which exhibited N= 4. Inhibition of the RyR1 activity from the cis side was also different from that of Gadolinium, characterized by Kd = 167 ? 5.0 nM and Nhill = 2.0 ? 0.1. The Eu3+ inhibition was potential independent if the charge carrier moved according to the physiological direction of calcium release, while it was potential dependent (and proportional with the driving force) if the current was opposite to the current during calcium release. We assume, that theEuropium binding site is in or near to channel pore because of voltage dependence of the Europium blockade. Effect of Europium was similar on the Ryanodine binding of HSR vesicles with slightlydifferent affinity probably due to the unspecific Europium binding by the associated junctional proteins. Ryanodine exerted its characteristic effect locking the channel into its half-conductance stateon the Europium modified channel independently that Europium was applied from the cis or from the trans side. A model explaining these data - considering the different ionic diameters for calcium,gadolinium and europium - is proposed to explain these findings and to propose further investigation of the ion conducting pore of the RyR1, based on the different ion-diameters of the lanthanides
Tárgyszavak:Természettudományok Biológiai tudományok idézhető absztrakt
calcium release
Megjelenés:Biophysical Journal. - Volume 94 : Suppl. 2 (2008), p. 426a. -
További szerzők:Topcsiov Zsanett Szabó Anett (élettanász) Jóna István (1948-) (élettanász, fizikus)
Pályázati támogatás:T061442
OTKA
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3.

001-es BibID:BIBFORM049386
Első szerző:Almássy János (élettanász, biológus, angol-magyar szakfordító)
Cím:Effect of scorpion toxins on the CRC/RyR function / Janos Almassy, Balazs Lukacs, Sandor Sarkozi, Istvan Jona
Dátum:2012
ISSN:0006-3495
Megjegyzések:It was shown previously that maurocalcin (MCa) induces long lasting subconductance states (LLSS) investigating the RyR function by single channel electrophysiology. These LLSSs are polarity and potential dependent and caused by the distinct positively charged surface formed by 5 amino acids corresponding to the peptide A binding site. We tested the effect of beta scorpion toxins - having a similar structure - on the RyR1 function. Charibdotoxin (CHTX) elicits close state at 20 nM in an all or none and voltage dependent manner because of smaller surface charge. Smaller size makes it easier to reach the most inner toxin binding site (out of the three) which causes the closure of the channel. MCa and CHTX share a common binding site which is identical to the peptide A binding site. Noxiustoxin has a similar effect at slightly higher toxin concentration. At nanomolar concentration Kaliotoxin evokes "flickering" of the channel in subconductance state which is occasionally interrupted by long lasting closed states, while locks the channel in closed state at micromolar concentration. Iberiotoxin induces a slight increase of the open probability accompanied by normal gating while Slotoxin has no effect. With the exception of MCa all toxins are effective only at one side, at the preferred side. Iberiotoxin and Slotoxin - ion spite of similar structure - have no large positive surfaces, they exhibit random surface charge distribution. A model has been proposed for the possible mode of action which accounts for the above effect of the tested toxins. Supported by Hungarian Research Found OTKA 81923.1563-PosB333
Tárgyszavak:Természettudományok Biológiai tudományok idézhető absztrakt
calcium release
Megjelenés:Biophysical Journal. - 102 : 3 (2012), p. 306a-307a. -
További szerzők:Lukács Balázs (1978-) (élettanász) Sárközi Sándor (1966-) (élettanász) Jóna István (1948-) (élettanász, fizikus)
Pályázati támogatás:81923
OTKA
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4.

001-es BibID:BIBFORM049383
Első szerző:Almássy János (élettanász, biológus, angol-magyar szakfordító)
Cím:Apical Ca2+ activated K+ channels in mouse lacrimal acinar cells / Janos Almassy, David I. Yule
Dátum:2013
Megjegyzések:During the contraction of a skeletal muscle fiber an action potential running along the fiber surface membrane results in the conformational change of the dihydropyridine receptors which in turn causes the opening of the sarcoplasmic reticulum (SR) calcium channels (RyR1). Calcium ions - released from the SR through the channels ? increase the myoplasmic calcium concentration that finally evokes the contraction of the fibre. The decrease in the myoplasmic calcium concentration causing relaxation of the fiber is achieved by the action of the SR Ca2+-ATPase (SERCA).It's known from the literature and our earlier results that thymol and its structural analogues ? which are widely used in the food, cosmetic and pharmaceutical industry as preservatives ? have influence on the activity of the RyR1 and SERCA. Continuing our previous work our aim was to study the effect of further phenol derivatives on the SERCA.Light SR (LSR) vesicles were prepared from rabbit skeletal muscle (m. longissimus dorsi) containing the SR Ca2+-pump in their membrane. ATP dependent hydrolytic activity of LSR vesicles was measured using "coupled enzyme assay" at 37oC. Specific activity of SERCA was calculated after determination of the non-specific activity in the presence of 20 ?M cyclopiazonic acid which specifically blocks SERCA.Pump activities were plotted against the concentration values of different drugs, dots were fitted by Hill-equation revealing the following parametes:4-Chloro-meta-crezol IC50=167 ? 8 ?M, nHill ~3;5-Chloro-orho-crezol IC50=554 ? 45 ?M, nHill ~2,4-Chloro-ortho-crezol IC50=1370 ? 88 ?M, nHill ~8.Almost all the compounds investigated here inhibited the pump except cresol which didn't exert any effect in concentration range 0?3 mM. Other compounds inhibited SERCA activity, but affinity and the number of ligands differed from each other.Our results prove that phenol derivative structural analogues have an inhibitory effect on SERCA activity but this effect is significantly modified by the relative position of the different substituent groups and the presence of cloride is also required for inhibition. The alterations in the shape of the pi electron cloud caused by the different substituents can be also involved in the effect of these compounds.Supported by: OTKA 81923
Tárgyszavak:Természettudományok Biológiai tudományok idézhető absztrakt
calcium release
calcium pump
Lacrimal Acinar Cell
Megjelenés:Biophysical Journal. - 104 : 2 (2013), p. 474a. -
További szerzők:Yule, David I.
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5.

001-es BibID:BIBFORM004700
Első szerző:Bagdány Miklós
Cím:Non-random distribution of Kv1.3 channels in the lymphocyte plasma membrane / Bagdany, M., Varga, S., Szentesi, G., Bodnar, A., Jenei, A., Damjanovich, S., Gaspar, R., Panyi, G.
Dátum:2002
Tárgyszavak:Orvostudományok Elméleti orvostudományok idézhető absztrakt
Megjelenés:Biophysical Journal. - 82 : 1 (2002), p. 1212. -
További szerzők:Varga Sándor (1943-) (biofizikus) Szentesi Gergely (1976-) (kémia-fizika tanár) Dóczy-Bodnár Andrea (1970-) (biofizikus) Jenei Attila (1966-) (biofizikus) Damjanovich Sándor (1936-2017) (biofizikus) Gáspár Rezső (1944-) (biofizikus) Panyi György (1966-) (biofizikus)
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6.

001-es BibID:BIBFORM004856
Első szerző:Bagossi Péter (biokémikus, vegyész)
Cím:Molecular modeling of nearly full-length ErbB2 receptor / Bagossi, P., Horvath, G., Vereb, G., Szollosi, J., Tozser, J.
Dátum:2005
ISSN:0006-3495
Megjegyzések:Members of the epidermal growth factor receptor family play important roles in various cellular processes, both in physiological and in pathological conditions. Dimerization and autophosphorylation of these receptor tyrosine kinases are key events of signal transduction. Details of the molecular events of the signaling are not entirely known. To facilitate the understanding of receptor structure and function at the molecular level, a molecular model was built for the nearly full-length ErbB2 dimer. Modeling was based on the x-ray or nuclear-magnetic resonance structures of extracellular, transmembrane, and intracellular domains. The extracellular domain was positioned above the cell membrane based on the distance determined from experimentally measured fluorescence resonance energy transfer. Favorable dimerization interactions are predicted for the extracellular, transmembrane, and protein kinase domains in the model of a nearly full-length dimer of ErbB2, which may act in a coordinated fashion in ErbB2 homodimerization, and also in heterodimers of ErbB2 with other members of the ErbB family
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
analysis
Cell Line, Tumor
Cell Membrane
chemistry
Comparative Study
Computer Simulation
Dimerization
Energy Transfer
Epidermal Growth Factor
Fluorescence
Fluorescence Resonance Energy Transfer
Humans
Hungary
Lipid Bilayers
methods
Models, Chemical
Models, Molecular
Neoplasms
Protein Conformation
Protein Structure, Tertiary
Receptor, erbB-2
Research
Signal Transduction
Structure-Activity Relationship
Support
ultrastructure
Megjelenés:Biophysical Journal. - 88 : 2 (2005), p. 1354-1363. -
További szerzők:Horváth Gábor (1974-) (biofizikus) Vereb György (1965-) (biofizikus, orvos) Szöllősi János (1953-) (biofizikus) Tőzsér József (1959-) (molekuláris biológus, biokémikus, vegyész)
Internet cím:elektronikus változat
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7.

001-es BibID:BIBFORM084527
035-os BibID:(WoS)000375141600351
Első szerző:Balajthy András (általános orvos)
Cím:7-Dehydrocholesterol Modifies the Operation of Kv1.3 Channels in T Cells Isolated from Smith-Lemli-Opitz Syndrome Patients / Balajthy Andras, Petho Zoltan, Somodi Sandor, Varga Zoltan, Peter Maria, Vígh Laszlo, Szabó Gabriella P., Paragh Gyorgy, Panyi Gyorgy, Hajdu Peter
Dátum:2016
ISSN:0006-3495
Tárgyszavak:Orvostudományok Klinikai orvostudományok idézhető absztrakt
folyóiratcikk
Megjelenés:Biophysical Journal. - 110 : 3 (2016), p. 278a-279a. -
További szerzők:Pethő Zoltán (1989-) (orvos) Somodi Sándor (1977-) (belgyógyász) Varga Zoltán (1969-) (biofizikus, szakfordító) Péter Mária Vígh László (orvos Szeged) P. Szabó Gabriella (1975-) (csecsemő- és gyermekgyógyász, klinikai genetikus) Paragh György (1953-) (belgyógyász) Panyi György (1966-) (biofizikus) Hajdu Péter (1975-) (biofizikus)
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8.

001-es BibID:BIBFORM083084
Első szerző:Bányász Tamás (élettanász)
Cím:Individual Cell Electrophysiology (ICE) of Cardiac Myocytes _ Using Innovative 'Onion-Peeling' Technique to Study the ICE of Excitable Cells / Banyasz Tamas, Jian Zhong, Horvath Balazs, Izu Leighton T., Chen-Izu Ye
Dátum:2012
ISSN:0006-3495
Tárgyszavak:Orvostudományok Elméleti orvostudományok idézhető absztrakt
folyóiratcikk
Megjelenés:Biophysical Journal. - 102 : 3 (2012), p. 544a. -
További szerzők:Jian, Zhong Horváth Balázs (1981-) (élettanász) Izu, Leighton T. Chen-Izu, Ye
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9.

001-es BibID:BIBFORM001017
Első szerző:Bányász Tamás (élettanász)
Cím:A new approach to the detection and statistical classification of Ca2+ sparks / Bányász T., Chen-Izu Y., Balke C. W., Izu L. T.
Dátum:2007
Megjegyzések:The availability of high-speed, two-dimensional (2-D) confocal microscopes and the expanding armamentarium of fluorescent probes presents unprecedented opportunities and new challenges for studying the spatial and temporal dynamics of cellular processes. The need to remove subjectivity from the detection process, the difficulty of the human eye to detect subtle changes in fluorescence in these 2-D images, and the large volume of data produced by these confocal microscopes call for the need to develop algorithms to automatically mark the changes in fluorescence. These fluorescence signal changes are often subtle, so the statistical estimate of the likelihood that the detected signal is not noise is an integral part of the detection algorithm. This statistical estimation is fundamental to our new approach to detection; in earlier Ca(2+) spark detectors, this statistical assessment was incidental to detection. Importantly, the use of the statistical properties of the signal local to the spark, instead of over the whole image, reduces the false positive and false negative rates. We developed an automatic spark detection algorithm based on these principles and used it to detect sparks on an inhomogeneous background of transverse tubule-labeled rat ventricular cells. Because of the large region of the cell surveyed by the confocal microscope, we can detect a large enough number of sparks to measure the dynamic changes in spark frequency in individual cells. We also found, in contrast to earlier results, that cardiac sparks are spatially symmetric. This new approach puts the detection of fluorescent signals on a firm statistical foundation.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Biophysical Journal 92 (2007), p. 4458-4465. -
További szerzők:Chen-Izu, Ye Balke, C. William Izu, Leighton T.
Internet cím:elektronikus változat
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10.

001-es BibID:BIBFORM049426
Első szerző:Bene László (biofizikus)
Cím:Intensity correlation-based calibration of FRET / László Bene, Tamás Ungvári, Roland Fedor, László Sasi Szabó, László Damjanovich
Dátum:2013
ISSN:0006-3495
Megjegyzések:ABSTRACT Dual-laser flow cytometric resonance energy transfer (FCET) is a statistically efficient and accurate way of determiningproximity relationships for molecules of cells even under living conditions. In the framework of this algorithm, absolutefluorescence resonance energy transfer (FRET) efficiency is determined by the simultaneous measurement of donor-quenchingand sensitized emission. A crucial point is the determination of the scaling factor a responsible for balancing the differentsensitivities of the donor and acceptor signal channels. The determination of a is not simple, requiring preparation of specialsamples that are generally different from a double-labeled FRET sample, or by the use of sophisticated statistical estimation(least-squares) procedures. We present an alternative, free-from-spectral-constants approach for the determination of a andthe absolute FRET efficiency, by an extension of the presented framework of the FCET algorithm with an analysis of the secondmoments (variances and covariances) of the detected intensity distributions. A quadratic equation for a is formulated withthe intensity fluctuations, which is proved sufficiently robust to give accurate a-values on a cell-by-cell basis in a wide systemof conditions using the same double-labeled sample from which the FRET efficiency itself is determined. This seemingly newapproach is illustrated by FRET measurements between epitopes of the MHCI receptor on the cell surface of two cell lines,FT and LS174T. The figures show that whereas the common way of a determination fails at large dye-per-protein labeling ratiosof mAbs, this presented-as-new approach has sufficient ability to give accurate results. Although introduced in a flow cytometer,the new approach can also be straightforwardly used with fluorescence microscopes.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
FRET
statistically efficient
Megjelenés:Biophysical Journal. - 105 : 9 (2013), p. 2024-2035. -
További szerzők:Ungvári Tamás Fedor Roland (1975-) (sebész) Sasi Szabó László András (1974-) (sebész) Damjanovich László (1960-) (általános sebész)
Pályázati támogatás:TÁMOP-4.2.2.A-11/1/KONV-2012-0045
TÁMOP
No. ASTF No 201-06
Egyéb
Short-term EMBO fellowship
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11.

001-es BibID:BIBFORM072608
035-os BibID:(WoS)000430439600694
Első szerző:Collet, Claude
Cím:Subcellular calcium events and calcium waves in leg skeletal muscle fibers isolated from the honey bee APIS Mellifera / C. Collet, C. Simut, M. Takacs, L. Szabo, P. Szentesi, L. Csernoch
Dátum:2018
ISSN:0006-3495
Tárgyszavak:Orvostudományok Elméleti orvostudományok idézhető absztrakt
folyóiratcikk
Megjelenés:Biophysical Journal. - 114 (2018), p. 289a. -
További szerzők:Simut, Cecilia Takács Marianna (1991-) (állattenyésztés) Szabó László József (1955-) (ökológus, hidrobiológus) Szentesi Péter (1967-) (élettanász) Csernoch László (1961-) (élettanász)
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12.

001-es BibID:BIBFORM040113
Első szerző:Collet, Claude
Cím:Intramembrane charge movement and L-type calcium current in skeletal muscle fibers isolated from control and mdx mice / Collet, C., Csernoch, L., Jacquemond, V.
Dátum:2003
ISSN:0006-3495
Megjegyzések:Dystrophin-deficient muscle fibers from mdx mice are believed to suffer from increased calcium entry and elevated submembranous calcium level, the actual source and functional consequences of which remain obscure. Here we compare the properties of the dihydropyridine receptor as voltage sensor and calcium channel in control and mdx muscle fibers, using the silicone-voltage clamp technique. In control fibers charge movement followed a two-state Boltzmann distribution with values for maximal charge, midpoint voltage, and steepness of 23 +/- 2 nC/ micro F, -37 +/- 3 mV, and 13 +/- 1 mV (n = 7). Essentially identical values were obtained in mdx fibers and the time course of charge recovery from inactivation was also similar in the two populations (tau approximately 6 s). In control fibers the voltage dependence of the slow calcium current elicited by 100-ms-long pulses gave values for maximal conductance, apparent reversal potential, half-activation potential, and steepness factor of 156 +/- 15 S/F, 65.5 +/- 2.9 mV, -0.76 +/- 1.2 mV, and 6.2 +/- 0.5 mV (n = 17). In mdx fibers, the half-activation potential of the calcium current was slightly more negative (-6.2 +/- 1.2 mV, n = 16). Also, when using longer pulses, the time constant of calcium current decay was found to be significantly larger (by a factor of 1.5-2) in mdx than in control fibers. These changes in calcium current properties are unlikely to be primarily responsible for a dramatic alteration of intracellular calcium homeostasis. They may be speculated to result, at least in part, from remodeling of the submembranous cytoskeleton network due to the absence of dystrophin.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Biophysical Journal. - 84 : 1 (2003), p. 251-265. -
További szerzők:Csernoch László (1961-) (élettanász) Jacquemond, Vincent
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