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001-es BibID:	BIBFORM027915
Első szerző:	Gorovits, Rena
Cím:	A mutation within the catalytic domain of COT1 kinase confers changes in the presence of two COT1 isoforms and in Ser/Thr protein kinase and phosphatase activities in Neurospora crassa / Rena Gorovits, Oshrat Propheta, Mikhail Kolot, Viktor Dombradi, Oded Yarden
Dátum:	1999
Megjegyzések:	Neurospora crassa grows by forming spreading colonies. cot-1 belongs to a class of N. crassa colonial temperature-sensitive (cot) mutants and encodes a Ser/Thr protein kinase. We have mapped the cot-1 mutation to a single base change resulting in a His to Arg substitution at amino acid 351, which resides within the catalytic domain. Antibodies raised against COT1 detected and immunoprecipitated a predominant 73-kDa polypeptide in N. crassa extracts, whose abundance was constant under all growth conditions tested. An additional, lower MW COT1 isoform (67-kDa) present in the wild-type was not detected in cot-1 grown at the restrictive temperature. Similarly, this isoform was not detected in cot-3 or cot-5 strains, when grown at restrictive temperatures. Reduced levels of Ser/Thr kinase activity and an increase in type 1 and type 2B phosphatase (calcineurin) activities were measured in a cot-1 background. Apparent changes in the phosphorylation state of the p150(Glued) subunit of the dynactin cytoskeletal motor component (encoded by ro-3, a suppressor of cot-1) and evidence of in vitro physical interactions between COT1 and calcineurin indicate a functional linkage among COT1 kinase, type 2B phosphatase, and dynactin.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban COT1 Ser/Thr protein kinase Neurospora crassa
Megjelenés:	Fungal Genetics and Biology. - 27 : 2-3 (1999), p. 264-274. -
További szerzők:	Propheta, Oshrat Kolot, Mikhail Yarden, Oded Dombrádi Viktor (1953-) (biokémikus)
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat DOI
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001-es BibID:	BIBFORM036845
035-os BibID:	PMID:22750657
Első szerző:	Leiter Éva (biológus)
Cím:	Protein phosphatase Z modulates oxidative stress response in fungi / Éva Leiter, Asier González, Éva Erdei, Carlos Casado, László Kovács, Csaba Ádám, Judit Oláh, Márton Miskei, Monika Molnar, Ilona Farkas, Zsuzsanna Hamari, Joaquín Ariño, István Pócsi, Viktor Dombrádi
Dátum:	2012
ISSN:	1087-1845
Megjegyzések:	The genome of the filamentous fungus Aspergillus nidulans harbors the gene ppzA that codes for the catalytic subunit of protein phosphatase Z (PPZ), and the closely related opportunistic pathogen Aspergillus fumigatus encompasses a highly similar PPZ gene (phzA). When PpzA and PhzA were expressed in Saccharomyces cerevisiae or Schizosaccharomyces pombe they partially complemented the deleted phosphatases in the ppz1 or the pzh1 mutants, and they also mimicked the effect of Ppz1 overexpression in slt2 MAP kinase deficient S. cerevisiae cells. Although ppzA acted as the functional equivalent of the known PPZ enzymes its disruption in A. nidulans did not result in the expected phenotypes since it failed to affect salt tolerance or cell wall integrity. However, the inactivation of ppzA resulted in increased sensitivity to oxidizing agents like tert-butylhydroperoxide, menadione, and diamide. To demonstrate the general validity of our observations we showed that the deletion of the orthologous PPZ genes in other model organisms, such as S. cerevisiae (PPZ1) or Candida albicans (CaPPZ1) also caused oxidative stress sensitivity. Thus, our work reveals a novel function of the PPZ enzyme in A. nidulans that is conserved in very distantly related fungi.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban Aspergillus (Emericella) nidulans protein phosphatase Z oxidative stress tert-butylhydroperoxide menadione diamide Molekuláris Medicina egyetemen (Magyarországon) készült közlemény
Megjelenés:	Fungal Genetics and Biology 49 : 9 (2012), p. 708-716. -
További szerzők:	Gonzalez, Asier Erdei Éva (1983-) (biológus, biotechnológus) Casado, Carlos Kovács László (1982-) (Ph.D. hallgató, molekuláris biológus) Ádám Csaba (1980-) (molekuláris biológus) Oláh Judit Miskei Márton (1978-) (molekuláris biológus, genetikus) Molnár Mónika (biofizikus) Farkas Ilona (1953-) (biokémikus) Hamari Zsuzsanna Ariño, Joaquín Pócsi István (1961-) (vegyész) Dombrádi Viktor (1953-) (biokémikus)
Pályázati támogatás:	TÁMOP-4.2.1/B-09/1/KONV-2010-0007 TÁMOP Jelátviteli folyamatok vizsgálata patogén gombákban
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM006471
Első szerző:	Miskei Márton (molekuláris biológus, genetikus)
Cím:	Annotation of stress-response proteins in the aspergilli / Márton Miskei, Zsolt Karányi, István Pócsi
Dátum:	2009
Megjegyzések:	Stress-response proteins of Aspergillus nidulans, Aspergillus clavatus, Aspergillus flavus, Aspergillus fumigatus, Aspergillus niger, Aspergillus oryzae, Aspergillus terreus and Neosartorya fischeri (3908 in total) were annotated and grouped according to stress types (http://193.6.155.82/AspergillusStress/). All genomes harboured elements of the SskA-HogA/SakA stress signalling pathway. There are accumulating data pointing at the importance of SskA-HogA/SakA signalling in different types of stress-responses in the aspergilli and, in this regard, these filamentous fungi are closer to fission yeast than to budding yeast. The abundance of annotated stress sensing histidine kinases and transcriptional regulators in each Aspergillus species indicates that the applicability of yeast-based models to fully describe and explain the stress-responses of these fungi is limited. Most excitingly, putative orthologues of both Saccharomyces cerevisiae Msn2p/Msn4p C2H2 zinc finger-type and Schizosaccharomyces pombe Atf1 bZip-type 1general stress' transcription factors were annotated in the aspergilli, foreshadowing complex and robust stress defence systems in these euascomycetes.
Tárgyszavak:	Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban Aspergillus, Stress-response Mitogen-activated protein kinase Environmental stress-response HOG pathway Osmotic stress Genome annotation Comparative genomics Neurospora crassa Schizosaccharomyces pombe Saccharomyces cerevisiae DNA repair Starvation stress Unfolded protein response Thermal stress Oxidative stress
Megjelenés:	Fungal Genetics and Biology 46 : 1 Suppl.1 (2009), p. 105-120. -
További szerzők:	Karányi Zsolt (1961-) (biostatisztikus, bioinformatikus) Pócsi István (1961-) (vegyész)
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