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1.

001-es BibID:BIBFORM023074
Első szerző:Celik-Ozenci, Ciler
Cím:Sperm selection for ICSI : shape properties do not predict the absence or presence of numerical chromosomal aberrations / Ciler Celik-Ozenci, Attila Jakab, Tamas Kovacs, Jillian Catalanotti, Ramazan Demir, Patricia Bray-Ward, David Ward, Gabor Huszar
Dátum:2004
ISSN:0268-1161
Megjegyzések:We hypothesize that the potential relationship between abnormal sperm morphology and increased frequency of numerical chromosomal aberrations is based on two attributes of diminished sperm maturity: (i) cytoplasmic retention and consequential sperm shape abnormalities; and (ii) meiotic errors caused by low levels of the HspA2 chaperone, a component of the synaptonemal complex. Because sperm morphology and aneuploidies were assessed in semen, but not in the same spermatozoa, previous studies addressing this relationship were inconclusive. We recently demonstrated that sperm shape is preserved following fluorescence in situ hybridization (FISH). Thus, we examined the shape and chromosomal aberrations in the same sperm. METHODS: We performed phase contrast microscopy and FISH, using centromeric probes for chromosomes X, Y, 10, 11 and 17 in 15 men. The fluorescence and respective phase contrast images were digitized using the Metamorph program. We studied 1286 sperm (256 disomic, 130 diploid and 900 haploid sperm) by three criteria: head and tail dimensions, head shape and Kruger strict morphology. Furthermore, in each analysis, we considered whether disomic or diploid sperm may be distinguished from haploid sperm. RESULTS: There was an overall, but not discriminative, relationship between abnormal sperm dimensions or shape and increased frequencies of numerical chromosomal aberrations. However, approximately 68 of the 256 disomic, and four of 130 diploid sperm showed head and tail dimensions comparable with the most normal, lowest tertile of the 900 haploid spermatozoa. Considering all 1286 sperm, among those with the most regular, symmetrical shape (n = 367), there were 63 and five with disomic and diploid nuclei, respectively. In line with these findings, among the 256 disomic sperm, 10% were Kruger normal. CONCLUSIONS: Sperm dimensions or shape are not reliable attributes in selection of haploid sperm for ICSI.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
spermium aneuploidia
Megjelenés:Human Reproduction. - 19 : 9 (2004), p. 2052-2059. -
További szerzők:Jakab Attila (1964-) (szülész-nőgyógyász, endokrinológus) Kovács Tamás (1959-) (szülész-nőgyógyász, humángenetikus) Catalanotti, Jillian Demir, Ramazan Bray-Ward, Patricia Ward, David Huszár Gábor
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2.

001-es BibID:BIBFORM046563
Első szerző:Jakab Attila (szülész-nőgyógyász, endokrinológus)
Cím:Efficacy of the swim-up method in eliminating sperm with diminished maturity and aneuploidy / Attila Jakab, Tamas Kovacs, Zoltan Zavaczki, Antal Borsos, Patricia Bray-Ward, David Ward, Gabor Huszar
Dátum:2003
ISSN:1460-2350
Megjegyzések:We have previously shown that after 80% Percoll centrifugation there is an overall 2.7-fold reduction of sperm with chromosomal disomies and diploidies (3.2-fold and 2.0-fold respectively), and of sperm with diminished maturity as detected by cytoplasmic retention. The relationship between disomies and immature sperm was r = 0.7, suggesting that disomy primarily originates in immature sperm. In the present work we studied the efficacy of the swim-up method in elimination of sperm with diminished maturity and with chromosomal aberrations in the swim-up sperm fractions of 10 patients (sperm concentration: 20 ? 3.9 x 106/ml, range 8.9-45.5; sperm motility: 45.2 ? 2.4, all mean ? SEM). METHODS: The validity of the study was enhanced by assessing each sperm fraction with three-colour (X, Y and 17; 5000 sperm) and two-colour (10 and 11; 5000 sperm) chromosome probes using fluorescence in-situ hybridization (FISH). Thus, in each sample 10 000 sperm were evaluated. The incidence of diminished maturity sperm was assessed with creatine kinase immunocytochemistry. RESULTS: In the swim-up fractions there was a reduction in the frequencies of disomic sperm, whether considering the sex chromosomes (1.4-fold) or the three autosomal chromosomes (1.5-fold based on the aggregate frequencies of disomy 10, 11 and 17). There was also a 1.5-fold reduction in diminished maturity sperm, indicating a relationship between the proportion of immature sperm and chromosomal aneuploidies (r = 0.46, P < 0.05, n = 20). Diploid sperm were reduced at a 2.7-fold rate, whether assessed with two- or three-colour FISH. There was a slight increase in the X/Y ratios. CONCLUSIONS: Swim-up reduces the proportion of sperm with chromosomal aberrations and of sperm with diminished maturity. When compared with the results of the previous study with gradient centrifugation performed on semen samples with similar quality, the efficacy after swim-up is lower for disomies and higher for diploidies than that of gradient centrifugation.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Human Reproduction. - 18 : 7 (2003), p. 1481-1488. -
További szerzők:Kovács Tamás (1959-) (szülész-nőgyógyász, humángenetikus) Zavaczki Zoltán Borsos Antal (1939-) (szülész-nőgyógyász, gyermeknőgyógyász) Bray-Ward, Patricia Ward, David Huszár Gábor
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3.

001-es BibID:BIBFORM061576
Első szerző:Jakab Attila (szülész-nőgyógyász, endokrinológus)
Cím:Correlation between sperm chromatin decondensation, density, aneuploidy and hyaluronic binding capacity / Jakab, A., Benyó, M., Mokanszki, A., Bazsáné, K. Z., Molnár, Z.
Dátum:2015
ISSN:0268-1161
Megjegyzések:Study question: Whether there is relation between sperm chromatin deconden- sation, concentration, hialuronic acid (HA) binding capacity and aneuploidy of semen samples of fertile, normozoospermic and infertile, oligozoospermic men?Summary answer: There are correlations between sperm condensation and the examined biomarkers, irrespective to fertility status. The sperm conden- sation rate, HA bindig capacity related fertility potential and sperm density are strongly related. Also, there is relation between sperm condensation and aneuploidy rate.What is known already: Importance of sperm biomarkers are increasing in male infertility. Beside genetic integrity and fertilization potential tests, epigen- etic studies came to the front. Chromatin decondensation is related to persistent histones detected by aniline blue, while HA binding capacity reflects genetic integrity and maturity related fertilization potential. Paralel evaluation of test results improves the understanding the regulation mechanisms of spermatogen- esis and fertilization, may help in choosing the most effective therapeutic ap- proach and sperm selection for assisted reproduction.Study design, size, duration: This is a cross-sectional study examining semen of 20 infertile, oligozoospermic and 20 fertile, normozoospermic men attend- ing at the Andrology Center and the Sperm Cryopreservation Laboratory. Participants/materials, setting, methods: The ejaculates were assessed as per WHO guidelines. Hyaluronic acid binding capacity test was the func- tional test. To determine the numerical chromosome aberrations we used multicolor fluorescent in situ hybridization (FISH) for chromosomes X, Y and 17. Aniline blue staining was performed as a nuclear decondensation marker test.Main results and the role of chance: In normozoospermic samples mean sperm concentration was 73,4 M/mL, rate of decondensed sperm nuclei detected by aniline blue 16.2%, HA-binding capacity 82% and estimated an- euploidy rate 4.12%. In the oligozoospermic samples mean sperm concentra- tion was 7.13 M/mL, rate of dencondensed nuclei 41.8%, HA binding capacity 47.5% and the estimated aneuploidy rate 6.17%. All examined parameters dif- fer significantly, if samples below and over 20% decondensation compared. There are inverse correlations between sperm decondensation and sperm con- centration (r = -0.876), as well as sperm decondensation and hyaluronic binding capacity (r = -0.876). Sperm decondensation rate is also related to aneuploidy rate (r = 0.559).Limitations, reason for caution: Data on confounders influencing sperm characteristics such as smoking, occupational or environmental hazards, co- morbidities were not collected.Wider implications of the findings: Although chromatin condensation occurs in late phase of spermatogenesis, it shows correlation with cytogenetic abnor- malities and membrane remodelling, irrespective to the fertility status. It can be explained with a common regulation mechanism, thus malfunction of it can result in various sperm defects on different levels. However, since examined biomarkers reflect different sperm defects, they cannot replace each other. Study funding/competing interest(s): Funding by national/international organization(s) ? This research was supported by the European Union and the State of Hungary, co-financed by the European Social Fund in the framework of TÁMOP 4.2.4.A/ 2-11/1-2012-0001Trial registration number: NA.Keywords: sperm function, sperm biomarkers, sperm decondensation, sperm aneuploidy, hyaluronic binding capacity
Tárgyszavak:Orvostudományok Klinikai orvostudományok idézhető absztrakt
sperm function
sperm biomarkers
sperm decondensation
sperm aneuploidy
sperm hyaluronic bindig capacity
Megjelenés:Human Reproduction. - 30 : Suppl (2015). p. i159. -
További szerzők:Benyó Mátyás (1982-) (urológus) Mokánszki Attila (1983-) (molekuláris biológus Ph.D hallgató) Bazsáné Kassai Zsuzsa (1946-) (embriológus) Molnár Zsuzsanna (1980-) (szülész-nőgyógyász, labor szakorvosjelölt, urológus)
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4.

001-es BibID:BIBFORM055584
035-os BibID:Article ID: P-008
Első szerző:Jakab Attila (szülész-nőgyógyász, endokrinológus)
Cím:Sperm fertilization potential and chromosomal anomalies at decreased sperm concentration : differences between testicular cancer patients and infertile men / A. Jakab, A. Mokánszki, A. Varga, M. Benyó, Z. Kassai, E. Oláh, Z. Molnár
Dátum:2013
ISSN:0268-1161
Megjegyzések:Study questionTo compare the sperm hyaluronic acid (HA) binding capacity and aneuploidy frequency of testicular germ cell carcinoma (TGCC) patients and infertile men without testicular cancer, but with decreased sperm concentrations.Summary answerWhile decreased fertilization potential reflected by low HA binding are related to low sperm count in both groups, elevated frequencies of chromosomal abnormalities are detected only in infertile, but not in TGCC patients. Qualitative spermiogenesis seems to be dissimilarly defected in TGCC and in infertile men without testicular cancer.What is known alreadyQuantitative spermatogenesis is known to be defected in men with TGCC, but little is known about the sperm quality and whether oligozoospermia arises from testicular alteration due the surgery or the tumor. Cryopreservation is the standard choice to preserve fertility. Infertile men with low sperm concentrations have higher frequency of aneuploid spermatozoa, which can be transmitted with assisted reproduction. HA binding capacity related to sperm maturity, viability, normal chromatin structure and fertilization potential.Study design, size, durationThis analysis is part of a prospective cohort study recruiting TGCC patients referred for cryopreservation and infertile men referred for genetic and functional sperm analysis in an academic tertiary center from 2010 to 2012. In this analysis patients with sperm count <40M/mL were included before treatment.Participants/materials, setting, methodsTwenty-one TGCC patients and 26 infertile men were examined. Spermiogram, Hyaluronan Binding Assay (HBA) was performed on native samples, HBA scores (%Bound = 100xBound Motile/Total Motile) were calculated. Sperm aneuploidy and diploidy frequency were examined with triple-color FISH scoring ca.5,000 spermatozoa of each individuals, then estimated numerical chromosome aberrations were calculated.Main results and the role of chanceMean sperm concentration was slightly higher (18.7 M/mL, range 6-35) in TGCC patients (mean age 28.7, range 18-37) than in infertile men (12.3 M/mL, range 1-39, mean age 34.4, 27-42) (p < 0.05). HBA score was 54.1% (range 8-86.5) in TGCC patients and even lower, 38.7% (0-83) in the infertile group (p < 0.05). The X/Y ratio were close to 1:1 in both groups. Estimated numerical chromosome aberrations were higher in the infertile than in the TGCC group (6.08% vs. 4.12%, p < 0.001), with elevations of both autosomal aneuploidies (0.11% vs. 0.07% of disomies 17, p < 0.001) and diploidies (0.30% vs. 0.15%, p < 0.001), but not sex chromosome disomies (0.41% vs. 0.40%, p = 0.1628). Samples normality was analysed using Shapiro-Wilk test, homogenity with Barlett test and the significance level with Two-sample t-probe.Limitations, reason for cautionMean sperm concentration is higher in TGCC patients than in the infertile group, since considering the limited amount for cryopreservation only samples < 6 M/mL were used for study purposes in those men.Wider implications of the findingsLow fertilization potential reflected in decreased sperm count and low hyaluronic binding capacity occurs in both TGCC and infertile patients. However, while the sperm aneuploidy in increased in infertile men, TGCC patients show similar rate to normal population. The presence of cancer does not increase the frequency of sperm numerical chromosomal aberrations. Thus, unlike in oligozoospermic infertile men, semen cryopreservation and assisted reproduction in TGCC do not increase risk of transmitting major genetic defects.Study funding/competing interest(s)Study was founded by the participating institutions and by the PhD scholarships. No competing interest declared.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idézhető absztrakt
sperm
testicular cancer
infertility
aneuploidy
hyaluronic binding capacity
Megjelenés:Human Reproduction. - 28 : S1 (2013), [2] p. -
További szerzők:Mokánszki Attila (1983-) (molekuláris biológus Ph.D hallgató) Varga Attila (1949-) (urológus, andrológus) Benyó Mátyás (1982-) (urológus) Bazsáné Kassai Zsuzsa (1946-) (embriológus) Oláh Éva (1943-2019) (gyermekgyógyász, klinikai genetikus) Molnár Zsuzsanna (1980-) (szülész-nőgyógyász, labor szakorvosjelölt, urológus)
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5.

001-es BibID:BIBFORM053792
Első szerző:Jakab Attila (szülész-nőgyógyász, endokrinológus)
Cím:Semen quality at cryobanking before oncologic treatment / A. Jakab, Z. Molnár, M. Benyó, I. Lévai, Z. Kassai
Dátum:2010
ISSN:0268-1161
Megjegyzések:Sperm cryobanking is a standard method of choice to preserve fertility of men undergoing oncologic treatment.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idézhető absztrakt
sperm cryopreservation
infertility
Megjelenés:Human Reproduction. - 26 (2010), p. i144-i145. -
További szerzők:Molnár Zsuzsanna (1980-) (szülész-nőgyógyász, labor szakorvosjelölt, urológus) Benyó Mátyás (1982-) (urológus) Lévai Írisz Bazsáné Kassai Zsuzsa (1946-) (embriológus)
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6.

001-es BibID:BIBFORM038992
Első szerző:Jakab Attila (szülész-nőgyógyász, endokrinológus)
Cím:Association between diploidy and double heads in spermatozoa arising from meiosis I and meiosis II / Jakab A., Kovacs T., Borsos A., Ward D. C., Bray-Ward P., Huszar G.
Dátum:2003
ISSN:0268-1161
Tárgyszavak:Orvostudományok Klinikai orvostudományok idézhető absztrakt
egyetemen (Magyarországon) készült közlemény
Megjelenés:Human Reproduction. - 18 : Suppl. 1. (2003), p. 188. -
További szerzők:Kovács Tamás (1959-) (szülész-nőgyógyász, humángenetikus) Borsos Antal (1939-) (szülész-nőgyógyász, gyermeknőgyógyász) Ward, David Bray-Ward, Patricia Huszár Gábor
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7.

001-es BibID:BIBFORM023067
Első szerző:Jakab Attila (szülész-nőgyógyász, endokrinológus)
Cím:The origin of sperm with extra chromosome set / Attila Jakab, Tamas Kovacs, Ciler Celik
Dátum:2002
ISSN:0268-1161
Tárgyszavak:Orvostudományok Klinikai orvostudományok szerkesztői levél
Megjelenés:Human Reproduction. - 18 : 2 (2002), p. 459. -
További szerzők:Kovács Tamás Ciler, Celik
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8.

001-es BibID:BIBFORM085810
035-os BibID:(cikkazonosító)101796
Első szerző:Lukács Luca (hallgató)
Cím:Phagocyte function of peripheral neutrophil granulocytes and monocytes in endometriosis before and after surgery / Lukács Luca, Kovács Anna Rebeka, Pál László, Szűcs Sándor, Kövér Ágnes, Lampé Rudolf
Dátum:2021
ISSN:2468-7847
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Megjelenés:Journal of Gynecology Obstetrics and Human Reproduction. - 50 : 4 (2021), p. 1-5. -
További szerzők:Kovács Anna Rebeka (1993-) (általános orvos) Pál László (1987-) (népegészségügyi szakember, egészségfejlesztő) Szűcs Sándor (1958-) (biokémikus, vegyész) Kövér Ágnes (1989-) (szociológus) Lampé Rudolf (1983-) (szülész-nőgyógyász)
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9.

001-es BibID:BIBFORM053794
Első szerző:Molnár Zsuzsanna (szülész-nőgyógyász, labor szakorvosjelölt, urológus)
Cím:Sperm fertilization potential and chromosomal anomalies in testicular cancer before chemotherapy / Z. Molnár, A. Mokánszki, M. Benyó, Z. Bazsáné Kassai, E. Oláh, A. Jakab
Dátum:2011
ISSN:0268-1161
Tárgyszavak:Orvostudományok Klinikai orvostudományok idézhető absztrakt
kemoterápia
spermium genetikai vizsgálat
Megjelenés:Human Reproduction. - 26 (2011), p. i253. -
További szerzők:Mokánszki Attila (1983-) (molekuláris biológus Ph.D hallgató) Benyó Mátyás (1982-) (urológus) Bazsáné Kassai Zsuzsa (1946-) (embriológus) Oláh Éva (1943-2019) (gyermekgyógyász, klinikai genetikus) Jakab Attila (1964-) (szülész-nőgyógyász, endokrinológus)
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10.

001-es BibID:BIBFORM053774
Első szerző:Molnár Zsuzsanna (szülész-nőgyógyász, labor szakorvosjelölt, urológus)
Cím:Sperm concentration, hyaluronic acid binding capacity, aneuploidy and persistent histones in testicular cancer / Z. Molnár, A. Mokanszki, Z. Kassai Bazsáné, H. P. Bhattoa, M. Benyó, É. Oláh, A. Jakab
Dátum:2014
ISSN:0268-1161
Megjegyzések:AbstractStudy question: Are the quantitative and qualitative characteristics of semen samples of patients with testicular cancer (TC), prior to anticancer therapy, different from infertile oligozoospermic and normozoospermic age-matched men?Summary answer: Sperm concentration in TC patients was significantly decreased with no difference in estimated numerical chromosome aberrations and nuclear decondensation compared to normozoospermic men, while the infertile, oligozoospermic men had significantly poorer sperm qualitative characteristics versus the TC group overall and oligozoospermic patients with testicular cancer.What is known already: Spermatogenesis is altered in TC patients at the time of the diagnosis. However, the mechanism responsible for the decreased semen quantity in patients with testicular cancer is not well understood. Anticancer treatment may have gonadotoxic side effects and post treatment fertility cannot be predicted. Before commencing anticancer treatment, cryopreservation may be suggested to preserve fertility but there are no data regarding the risk of genetic aberrations in these sperms.Study design, size, duration: This is a cross-sectional study examining semen from 28 patients with testicular cancer, 20 oligozoospermic and 20 normozoospermic age-matched men attending the Andrology Center and the Sperm Cryopreservation Laboratory of the Medical and Health Science Center, University of Debrecen. Semen samples from patients with testicular cancer were collected after orchidectomy, but prior to anticancer treatment. Semen samples from TC patients recruited over a period of 4 years were studied. Based on their sperm concentration, TC patients were subgrouped into an oligozoospermic TC (TCO) and a normozoospermic TC (TCN) group. For statistical analysis, the normal group (NZ+IO) comprised non-tumorous normozoospermic (NZ) and infertile oligozoospermic (IO) men.Participants/materials/setting, method: The ejaculates were assessed as per World Health Organization guidelines. Hyaluronic acid (HA) binding capacity was the functional test. To determine the numerical chromosome aberrations we used multicolour fluorescence in-situ hybridization. Aniline blue (AB) staining was performed as a nuclear decondensation marker test. Main results and the role of chance: The results did not reveal any significant difference in disomy of sex chromosomes and chromosome 17, diploidy and estimated numerical chromosome aberrations and AB staining results upon comparing the normozoospermic and testicular cancer groups, although the sperm concentration (p<0.001) and HA binding capacity (p<0.001) were lower in the TC group. Estimated numerical chromosome aberrations (p<0.001), AB staining (p<0.001) and HA-binding capacity (p=0.019) was lower in the infertile, oligozoospermic group as compared to the patients with testicular cancer. The TCO group had significantly better results in every examined parameter than the infertile, oligozoospermic group. In the non-tumorous control group (NZ+IO), a significant (p<0.001) correlation (Spearman's rho=r) was found between sperm concentration and aneuploidy rate (r=-0.642), AB staining (r=-0.876) and HA binding (r=0.842); the HA-binding capacity was related to the aneuploidy rate (r=-0.678) and the AB staining (r=-0.811,); and there was significant correlation between aneuploidy and AB staining (r=0.559). In the TC group, apart from the negative correlation between sperm concentration and estimated chromosomal aberrations (r=-0.642), no other correlations were observed.Limitations, reasons for caution: Data on confounders influencing sperm characteristics, such as smoking, occupational or environmental hazards, alcoholism, co-morbidities and other andrological conditions, were not collected. Wider implications of the findings: This is the first study to demonstrate that sperm qualitative characteristics in anticancer therapy naïve oligozoospermic TC patients differ significantly from those in infertile oligozoospermic men and do not differ from those in normozoospermic men. Our results need to be validated in similar groups of men and in other patient groups with cancer where cryopreservation is advisable. Study funding/competing interest(s): This research was supported by the European Union and the State of Hungary, co-financed by the European Social Fund in the framework of TÁMOP-4.2.4.A/2-11/1-2012-0001 ♭National Excellence Program'. The authors have no conflict of interest to declare.Key words: testicular cancer, infertility, hyaluronic acid binding assay, aniline-blue staining, human sperm fluorescence in-situ hybridization
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
testicular cancer
infertility
hyaluronic acid binding assay
aniline-blue staining
human sperm fluorescence in-situ hybridization
Megjelenés:Human Reproduction. - 29 : 9 (2014), p. 1866-1874. -
További szerzők:Mokánszki Attila (1983-) (molekuláris biológus Ph.D hallgató) Bazsáné Kassai Zsuzsa (1946-) (embriológus) Bhattoa Harjit Pal (1973-) (laboratóriumi szakorvos) Benyó Mátyás (1982-) (urológus) Oláh Éva (1943-2019) (gyermekgyógyász, klinikai genetikus) Jakab Attila (1964-) (szülész-nőgyógyász, endokrinológus)
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11.

001-es BibID:BIBFORM100559
035-os BibID:(cikkazonosító)102350
Első szerző:Riemma, Gaetano
Cím:The role of hysteroscopy in reproductive surgery : today and tomorrow / Riemma Gaetano, Vitale Salvatore Giovanni, Manchanda Rahul, Rathore Aayushi, Török Péter, De Angelis Carlo, Urman Bulent, Sareri Marco Iraci, La Verde Marco, Carugno Jose, De Franciscis Pasquale, Tesarik Jan
Dátum:2022
ISSN:2468-7847
Megjegyzések:During the last decades, the number of couples with reproductive issues has substantially increased. Many different factors are implicated in reproductive failure, including uterine factors. Endometrial pathologies, such as endometrial polyps, hyperplasia, endometritis, and Mullerian anomalies, can also hinder embryo implantation. Hysteroscopy remains the gold standard for the evaluation and treatment of intrauterine pathology. Over the last few years, advances in hysteroscopic instrumentations and surgical techniques have significantly evolved, the refinement in technology, miniaturization of instruments, and improved image quality have rendered hysteroscopy a more patient and user-friendly procedure that has enhanced its use in reproductive medicine. Nowadays, hysteroscopy is essential in the evaluation and treatment of women with infertility. This article underscores the major technological breakthroughs achieved over the last few years with emphasis on the role of artificial intelligence, augmented reality, and 3D hysteroscopy, which can set new benchmarks in hysteroscopy applied to reproductive medicine.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Hysteroscopy
portable hysteroscope
tissue retrieval system
3D hysteroscopy
clinical pregnancy
IVF
Megjelenés:Journal of Gynecology Obstetrics and Human Reproduction. - 51 : 4 (2022), p. 1-5. -
További szerzők:Vitale, Salvatore Giovanni Manchanda, Rahul Rathore, Aayushi Török Péter (1975-) (szülész-nőgyógyász) De Angelis, Carlo Urman, Bulent Sareri, Marco Iraci La Verde, Marco Carugno, Jose De Franciscis, Pasquale Tesarik, Jan
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12.

001-es BibID:BIBFORM071334
Első szerző:Török Péter (szülész-nőgyógyász)
Cím:Differentiating tissues and organs in endoscopic images using a convolutional neural network / Török Péter, Lampé Rudolf, Harangi Balázs, Lőrincz Judit
Dátum:2017
ISSN:0268-1161
Megjegyzések:TitleDifferentiating tissues and organs in endoscopic images using a convolutional neural networkStudy questionIs it possible to identify different tissues and organs during endoscopy supported by a software.Summary answer During the learning curve of laparoscopy trainees can get help in differentiating tissues by an automated digital image processing based decision support systemWhat is known already Endoscopy surgery is the part of everyday work of surgeon. In an experienced hand provides less postoperative morbidity, complications and the time periods of hospital stay and returning to normal activity, so it has a lot of advantages compared to the laparotomy.Opposing to open-surgery, the recognition of organs is rather difficult in laparoscopy because of the lack of the tactile information. Knowing detailed anatomy, having experience letting us to recognize structures in the abdominal cavity. Because of the anatomical variations, tissue identification is not always sure relying on the visual information.Study design, size, duration COur dataset has been collected retrospectively during 35 different gynecological endoscopic operations at the Department of Obstetrics and Gynecology of the University of Debrecen. The videos have been recorded by a high definition 1-MOS endoscopic camera at 30 frames/sec rate and resolution of 1920?1080 pixels.Participants/materials, setting, methods Data of patients scheduled for gynecological endoscopic operations are analyzed. The medical expert or an assistant should manually mark the region of interest. Then, the maximum number of sub-images of size 224?224 pixels are cut off along the axis from the video frame. Finally, the classification problem is solved automatically using a convolutional neural network with the resulted labels are pinned on the corresponding organs in the video frame.Main results and the role of chance We have presented an approach to develop an application, which helps medical experts with performing endoscopic surgeries. Our effort primarily addressed the drawback of losing tactile information during key-hole surgery in the recognition of different organs. To address this problem, we have developed a semi-automatic tool, which requires a manual annotation regarding the axis of the interested organs first. Then, several sub-images covering the selected organs are extracted and classified by a fine-tuned GoogLeNet convolutional neural network.The classification performance of the fine-tuned GoogLeNet model on our test dataset considering the top-1 error rate is 0.193 at sub-image level. That is, 403 out of the 500 test images have been classified correctly. However, notice that these sub-images are only small, non-overlapping segments of the interested organs. That is, it is reasonable to fuse these label information for recognizing the corresponding organ. To do so, we have applied the simple majority-voting rule on the 4-5 labels supplied by the sub-images for the same organ. In this way, our proposed approach has reached 94.2% final accuracy regarding this binary classification task.Limitations, reasons for cautionOur collected dataset is relatively small with containing insufficient number of images to train a complex neural network, so we should extend the size of our dataset.Wider implications of the findings Using the software made by the results of the study, accuracy of the tissue/organ recognition could be increased during training laparoscopic technique, or for the experts as well.Study funding/competing interest(s) This work was supported in part by the projects GINOP-2.1.1-15-2015-00376 and VKSZ 14-1-2015-0072, SCOPIA: Development of diagnostic tools based on endoscope technology supported by the European Union, co-financed by the European Social Fund.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idézhető absztrakt
laparoszkópia
endoszkópia
mélytanulás
konvolúcionális neurális hálózat
szövetfelismerés
Megjelenés:Human Reproduction 32 : suppl. 1 (2017), p. 487. -
További szerzők:Lampé Rudolf (1983-) (szülész-nőgyógyász) Harangi Balázs (1986-) (programtervező matematikus) Lőrincz Judit (1988-) (általános orvos)
Internet cím:DOI
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