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1.

001-es BibID:BIBFORM054375
Első szerző:Bánki Eszter
Cím:Molecular Mechanisms Underlying the Nephroprotective Effects of PACAP in Diabetes / Eszter Banki, Krisztina Kovacs, Daniel Nagy, Tamas Juhasz, Peter Degrell, Katalin Csanaky, Peter Kiss, Gabor Jancso, Gabor Toth, Andrea Tamas, Dora Reglodi
Dátum:2014
ISSN:0895-8696
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Journal of Molecular Neuroscience 54 : 3 (2014), p. 300-309. -
További szerzők:Kovács Krisztina (Pécs) Nagy Dániel Juhász Tamás (1976-) (biológus, orvosbiológus) Degrell Péter Csanaky Katalin Kiss Péter (Pécs) Jancsó Gábor Tóth Gábor Tamás Andrea (Idegtudomány) (Pécs) Reglődi Dóra (Idegtudományok)
Pályázati támogatás:K104984
OTKA
108596
OTKA
TÁMOP-4.2.2.A-11/1/KONV-2012-0024
TÁMOP
Bolyai Ösztöndíj
Egyéb
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2.

001-es BibID:BIBFORM003873
Első szerző:Boros Ákos
Cím:Changes in the expression of PACAP-like compounds during the embryonic development of the earthworm eisenia fetida / Akos Boros, Dora Reglodi, Zsofia Herbert, Gabor Kiszler, Jozsef Nemeth, Andrea Lubics, Peter Kiss, Andrea Tamas, Seiji Shioda, Kouhei Matsuda, Edit Pollak, Laszló Molnar
Dátum:2008
Megjegyzések:Pituitary adenylate cyclase-activating polypeptide (PACAP) is expressed at very early stages in the vertebrate nervous system, and its functions in the embryonic development have been shown by various studies. PACAP is an extremely conserved molecule in phylogeny; however, little is known about its presence and functions in invertebrates. Our previous studies have shown the occurrence of PACAP-like immunoreactivity in the invertebrate nervous system. The aim of this study was to investigate the presence and localization of PACAP-like compounds during the embryonic development of earthworms from cocoon deposition to hatching using immunological methods (radioimmunoassay, dot blot, immunohistochemistry). PACAP-like immunoreactive compounds were detected at very early stages of the embryonic development of the earthworm Eisenia fetida. No significant changes were observed during the early stages in the developing embryo, but a marked increase occurred before hatching. In contrast, during the embryonic development, the level of PACAP-like compounds gradually decreased in cocoon fluids. Immunohistochemistry revealed the presence of PACAP-like immunoreactive cell bodies and processes in the developing body wall, prostomium, pharyngeal wall, and central nervous system. Cells located in the body wall correspond to putative progenitor cells of primary sensory cells. In the present study, we also showed that the clitellum (reproductive organ) of sexually mature worms contained significantly higher levels of PACAP-like immunoreactivity than other regions of the same animals or the clitellar region of a non-reproducing animal. In summary, these observations provide a morphological basis and suggest a role of PACAP(-like peptides) in the reproductive and developmental functions of invertebrates.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Dot blot
Clitellum
Cocoon
Earthworm
embryo
Primary sensory cells
Radioimmunoassay
Megjelenés:Journal of molecular neuroscience. - 36 : 1-3 (2008), p. 157-165. -
További szerzők:Reglődi Dóra (Idegtudományok) Herbert Zsófia Kiszler Gábor Németh József (1954-) (vegyész, analitikus) Lubics Andrea (Pécs) Kiss Péter Tamás Andrea (Idegtudomány) (Pécs) Shioda, Seiji Matsuda, Kouhei Pollák Edit Molnár László
Internet cím:elektronikus változat
DOI
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3.

001-es BibID:BIBFORM013274
Első szerző:Brubel Réka
Cím:Changes in the Expression of Pituitary Adenylate Cyclase-Activating Polypeptide in the Human Placenta during Pregnancy and Its Effects on the Survival of JAR Choriocarcinoma Cells/ R. Brubel, A. Boronkai, D. Reglodi, B. Racz, J. Nemeth, P. Kiss, A. Lubics, G. Toth, G. Horvath, T. Varga, D. Szogyi, E. Fonagy, J. Farkas, A. Barakonyi, Sz. Bellyei, L. Szereday, M. Koppan, A. Tamas
Dátum:2010
Megjegyzések:Pituitary adenylate cyclase-activating polypeptide(PACAP), a neuropeptide with survival-promotingactions, has been observed in endocrine organs and isthought to play a role in reproductive functions, includingpregnancy. PACAP occurs in two forms, 27 and 38 aminoacid residues, with PACAP38 being the predominant formin human tissues. In the present study, we determined theconcentrations of PACAP38 and PACAP27 in firsttrimesterand full-term human placentas using radioimmunoassay.We found high levels of PACAP38 and lowerlevels of PACAP27 in different parts of the full-term humanplacenta. PACAP38 content increased in the placentaduring pregnancy, both on the maternal side and on thefetal side. The effects of PACAP on the survival of JARhuman choriocarcinoma cells were investigated using flowcytometry and MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) cell viability assay in cellsexposed to the widely used chemotherapeutic agentmethotrexate (MTX). It was found that PACAP neitherinfluenced the survival of JAR cytotrophoblast cells noraffected cellular response to the death-inducing effect of thechemotherapeutic agent MTX. The present observationsfurther support the significance of PACAP in the humanplacenta. The observation that PACAP did not influence theeffects of MTX may have future clinical importance,showing that PACAP does not decrease the effects ofcertain chemotherapeutic agents.Keywords JAR . Choriocarcinoma . RIA .
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
JAR
Choriocarcinoma
RIA
PACAP38
Megjelenés:Journal of Molecular Neuroscience. - 42 : 3 (2010), p. 450-458. -
További szerzők:Boronkai A. Reglődi Dóra (Idegtudományok) Rácz B. (Pécs) Németh József (1954-) (vegyész, analitikus) Kiss Péter (Pécs) Lubics Andrea (Pécs) Tóth Gábor (Szeged) Horváth Gábor (Pécs) Varga T. (Pécs) Szogyi D. (Pécs) Fonagy E. Farkas J. (Pécs) Barakonyi A. Bellyei Szabolcs Szereday László Koppán Miklós Tamás A. (Pécs)
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4.

001-es BibID:BIBFORM069485
Első szerző:Farkas József
Cím:Early Neurobehavioral Development of Mice Lacking Endogenous PACAP / Farkas J., Sandor B., Tamas A., Kiss P., Hashimoto H., Nagy A. D., Fulop B. D., Juhasz T., Manavalan S., Reglodi D.
Dátum:2017
Megjegyzések:Pituitary adenylate cyclase activating polypeptide (PACAP) is a multifunctional neuropeptide. In addition to its diverse physiological roles, PACAP has important functions in the embryonic development of various tissues, and it is also considered as a trophic factor during development and in the case of neuronal injuries. Data suggest that the development of the nervous system is severely affected by the lack of endogenous PACAP. Short-term neurofunctional outcome correlates with long-term functional deficits; however, the early neurobehavioral development of PACAP-deficient mice has not yet been evaluated. Therefore, the aim of the present study was to describe the postnatal development of physical signs and neurological reflexes in mice partially or completely lacking PACAP. We examined developmental hallmarks during the first 3 weeks of the postnatal period, during which period most neurological reflexes and motor coordination show most intensive development, and we describe the neurobehavioral development using a complex battery of tests. In the present study, we found that PACAP-deficient mice had slower weight gain throughout the observation period. Interestingly, mice partially lacking PACAP weighed significantly less than homozygous mice. There was no difference between male and female mice during the first 3 weeks. Some other signs were also more severely affected in the heterozygous mice than in the homozygous mice, such as air righting, grasp, and gait initiation reflexes. Interestingly, incisor teeth erupted earlier in mice lacking PACAP. Motor coordination, shown by the number of foot-faults on an elevated grid, was also less developed in PACAP-deficient mice. In summary, our results show that mice lacking endogenous PACAP have slower weight gain during the first weeks of development and slower neurobehavioral development regarding a few developmental hallmarks.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Knockout
Neurodevelopment
Neuropeptide
Reflex
Trophic factor
Megjelenés:Journal of molecular neuroscience. - 61 : 4 (2017), p. 468-478. -
További szerzők:Sándor Balázs Tamás Andrea (Idegtudomány) (Pécs) Kiss Péter (Pécs) Hashimoto, Hitoshi Nagy András Dávid Fülöp Balázs Dániel (Orvosi alapkutatások) Juhász Tamás (1976-) (biológus, orvosbiológus) Manavalan, Sridharan Reglődi Dóra (Idegtudományok)
Pályázati támogatás:K104984
OTKA
K119759
OTKA
GINOP-2.3.2-15-2016-00050
GINOP
TAMOP 4.2.4.A/2-11-1-2012-0001
TÁMOP
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5.

001-es BibID:BIBFORM008847
Első szerző:Ferencz Andrea
Cím:Influence of PACAP on oxidative stress and tissue injury following small-bowel autotransplantation / Ferencz A., Racz B., Tamas A., Reglodi D., Lubics A., Nemeth J., Nedvig K., Kalmar-Nagy K., Horvath O. P., Weber G., Roth E.
Dátum:2009
Megjegyzések:Abstract Tissue injury caused by cold preservation and reperfusion remains an unsolved problem during smallbowel transplantation. Pituitary adenylate cyclase-activating polypeptide (PACAP) is present and plays a central role in the intestinal physiology. This study investigated effect of PACAP-38 on the oxidative stress and tissue damage in autotransplanted intestine. Sham-operated, ischemia/reperfusion, and autotransplanted groups were established in Wistar rats. In ischemia/reperfusion groups, 1 h (group A), 2 h (group B), and 3 h (group C) ischemia followed by 3 h of reperfusion was applied. In autotransplanted groups, total orthotopic intestinal autotransplantation was performed. Grafts were preserved in University of Wisconsin (UW) solution and in UW containing 30 ?g PACAP-38 for 1, 2, 3, and 6 h. Reperfusion lasted 3 h in all groups. Endogenous PACAP-38 concentration was measured by radioimmunoassay. To determine oxidative stress parameters, malondialdehyde, reduced glutathione, and superoxide dismutase were measured in tissue samples. Tissue damage was analyzed by qualitative and quantitative methods on hematoxylin/eosinstained sections. Concentration of endogenous PACAP-38 significantly decreased in groups B and C compared to sham-operated group. Preservation solution containing PACAP-38 ameliorated bowel tissue oxidative injury induced by cold ischemia and reperfusion. Histological results showed that preservation caused destruction of the mucous, submucous, and muscular layers, which were further deteriorated by the end of reperfusion. In contrast, PACAPJ-38 significantly protected the intestinal structure. Ischemia/ reperfusion decreased the endogenous PACAP-38 concentration in the intestinal tissue. Administration of PACAP-38 mitigated the oxidative injury and histological lesions in small-bowel autotransplantation model.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Small-bowel transplantation
Megjelenés:Journal of Molecular Neuroscience. - 37 : 2 (2009), p. 168-176. -
További szerzők:Rácz Boglárka Tamás Andrea (Idegtudomány) (Pécs) Reglődi Dóra (Idegtudományok) Lubics Andrea (Pécs) Németh József (1954-) (vegyész, analitikus) Nedvig Klára Kalmár-Nagy Károly Horváth Örs Péter (sebész) Wéber György Rőth Erzsébet (1957-) (vegyész)
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6.

001-es BibID:BIBFORM103634
035-os BibID:(WOS)000472082700006 (Scopus)85051646952
Első szerző:Fülöp Balázs Dániel (Orvosi alapkutatások)
Cím:Altered Notch Signaling in Developing Molar Teeth of Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP)-Deficient Mice / B. D. Fulop, B. Sandor, E. Szentleleky, E. Karanyicz, D. Reglodi, B. Gaszner, R. Zakany, H. Hashimoto, T. Juhasz, A. Tamas
Dátum:2019
ISSN:0895-8696
Megjegyzések:Pituitary adenylate cyclase-activating polypeptide (PACAP) is a neuropeptide with neuroprotective and neurotrophic effects. This suggests its influence on the development of teeth, which are, similarly to the nervous system, ectoderm and neural crest derivatives. Our earlier studies have shown morphological differences between wild-type (WT) and PACAP-deficient mice, with upregulated sonic hedgehog (SHH) signaling in the lack of PACAP. Notch signaling is a key element of proper tooth development by regulating apoptosis and cell proliferation. In this study, our main goal was to evaluate the possible effects of PACAP on Notch signaling pathway. Immunohistochemical staining was performed of Notch receptors (Notch1, 2, 3, 4), their ligands [deltalike protein (DLL)1, 3, 4, Jagged1, 2], and intracellular target molecules [CSL (CBF1 humans/Su (H) Drosophila/LAG1 Caenorhabditis elegans transcription factor); TACE (TNF-? converting enzyme), NUMB] in molar teeth of 5-day-old WT, and homozygous and heterozygous PACAP-deficient mice. We measured immunopositivity in the enamel-producing ameloblasts and dentin-producing odontoblasts. Notch2 receptor and DLL1 expression were elevated in ameloblasts of PACAPdeficient mice compared to those in WT ones. The expression of CSL showed similar results both in the ameloblasts and odontoblasts. Jagged1 ligand expression was elevated in the odontoblasts of homozygous PACAP-deficient mice compared to WT mice. Other Notch pathway elements did not show significant differences between the genotype groups. The lack of PACAP leads to upregulation of Notch pathway elements in the odontoblast and ameloblast cells. The underlying molecular mechanisms are yet to be elucidated; however, we propose SHH-dependent and independent processes. We hypothesize that this compensatory upregulation of Notch signaling by the lack of PACAP could represent a salvage pathway in PACAP-deficient animals.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
PACAP-deficient mice
Notch signaling
DLL1, 3, 4, Jagged1, 2
Notch1, 2, 3, 4
CSL
TACE
NUMB
Development of molar tooth
Megjelenés:Journal of Molecular Neuroscience. - 68 : 3 (2019), p. 377-388. -
További szerzők:Sándor Balázs Szentléleky Eszter (fogászat) Karanyicz Edina Reglődi Dóra (Idegtudományok) Gaszner Balázs (Neuroanatómia) Zákány Róza (1963-) (anatómus-, kötőszövetbiológus) Hashimoto, Hitoshi Juhász Tamás (1976-) (biológus, orvosbiológus) Tamás Andrea (Idegtudomány) (Pécs)
Pályázati támogatás:2.3.2-15-2016-00050 BPEPSYS^
GINOP
3.6.3-VEKOP-16-15 2017-00008
EFOP
3.6.2-16-2017-00008
EFOP
3.6.1- 16.2016.00004
EFOP
4.2.4.A/2-11-1-2012-0001
TAMOP
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7.

001-es BibID:BIBFORM082526
035-os BibID:(PMID)31808034
Első szerző:Józsa Gergő
Cím:Lack of Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP) Disturbs Callus Formation / Gergő Józsa, Balázs Dániel Fülöp, László Kovács, Bernadett Czibere, Vince Szegeczki, Tamás Kiss, Tibor Hajdú, Andrea Tamás, Zsuzsanna Helyes, Róza Zákány, Dóra Reglődi, Tamás Juhász
Dátum:2021
ISSN:0895-8696
Megjegyzések:Pituitary adenylate cyclase-activating polypeptide (PACAP) is a naturally secreted signaling peptide and has important regulatory roles in the differentiation of the central nervous system and its absence results in disorders in femur development. PACAP has an important function in prevention of oxidative stress or mechanical stress in chondrogenesis but little is known about its function in bone regeneration. A new callus formation model was set to investigate its role in bone remodeling. Fracturing was 5 mm distal from the proximal articular surface of the tibia and the depth was 0.5 mm. Reproducibility of callus formation was investigated with CT 3, 7, and 21 days after the operation. Absence of PACAP did not alter the alkaline phosphatase (ALP) activation in PACAP KO healing process. In developing callus, the expression of collagen type I increased in wild-type (WT) and PACAP KO mice decreased to the end of healing process. Expression of the elements of BMP signaling was disturbed in the callus formation of PACAP KO mice, as bone morphogenic protein 4 (BMP4) and 6 showed an early reduction in bone regeneration. However, elevated Smad1 expression was demonstrated in PACAP KO mice. Our results indicate that PACAP KO mice show various signs of disturbed bone healing and suggest PACAP compensatory and fine tuning effects in proper bone regeneration.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
ALP
BMP
Callus
Collagen type I
PACAP
Tibia fracture
Megjelenés:Journal of Molecular Neuroscience. - 71 : 8 (2021), p. 1543-1555. -
További szerzők:Fülöp Balázs Dániel (Orvosi alapkutatások) Kovács László Czibere Bernadett Szegeczki Vince (1992-) Kiss Tamás Hajdú Tibor (1988-) (általános orvos) Tamás Andrea (Idegtudomány) (Pécs) Helyes Zsuzsanna Zákány Róza (1963-) (anatómus-, kötőszövetbiológus) Reglődi Dóra (Idegtudományok) Juhász Tamás (1976-) (biológus, orvosbiológus)
Pályázati támogatás:NKFIK115874
Egyéb
PD109644
Egyéb
K119759
Egyéb
NKFIHFK129190
Egyéb
GINOP-2.3.2-15-2016-00050
GINOP
UNKP-16-4-IV.
Egyéb
EFOP-3.6.1.-16-2016-00004
EFOP
EFOP-3.6.2-16-2017-00008
EFOP
EFOP-3.6.3-VEKOP-16-2017-00009
EFOP
EFOP-3.6.1.-16-2016-00004
EFOP
NAP B KTIA NAP 13-2014-0022
Egyéb
OTKA-NN 114458
OTKA
TÁMOP 4.2.4
TÁMOP
TÁMOP A/2-11-1-2012-0001
TÁMOP
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8.

001-es BibID:BIBFORM077126
Első szerző:Juhász Tamás (biológus, orvosbiológus)
Cím:Pituitary adenylate cyclase activating polypeptide (pacap)-signalling promotes chondrogenesis : implication of calcineurin as a downstream target / Juhasz T., Matta Cs., Katona E., Somogyi Cs., Takacs R., Kolozsvari B., Csernoch László, Panyi Gy., Tóth Gábor, Reglődi Dóra, Tamas Andrea, Zakany R.
Dátum:2013
ISSN:0895-8696
Tárgyszavak:Orvostudományok Elméleti orvostudományok idézhető absztrakt
Science & Technology
Life Sciences & Biomedicine
Biochemistry & Molecular Biology
Neurosciences
Neurosciences & Neurology
Biochemistry & molecular biology
Neurosciences
Elméleti Orvostudományok
Megjelenés:Journal Of Molecular Neuroscience. - 51 : Suppl. 1 (2013), p. 229. -
További szerzők:Matta Csaba (1980-) (molekuláris biológus, genetikus, angol szakfordító) Katona Éva (1986-) (molekuláris biológus) Somogyi Csilla (1983-) (biológus, angol-magyar szakfordító) Takács Roland Ádám (1985-) (molekuláris biológus, biokémikus) Kolozsvári Bernadett (1982-) (molekuláris biológus, genetikus) Csernoch László (1961-) (élettanász) Panyi György (1966-) (biofizikus) Tóth Gábor (Szeged) Reglődi Dóra (Idegtudományok) Tamás Andrea (Idegtudomány) (Pécs) Zákány Róza (1963-) (anatómus-, kötőszövetbiológus)
Pályázati támogatás:CNK80709
OTKA
TÁMOP 4.2.1.B-10/2/KONV-2010-002
TÁMOP
TÁMOP 4.2.2.A-11/1/KONV-2012-0025
TÁMOP
TAMOP 4.2.2.A-11/1/KONV-2012-0024
TÁMOP
TAMOP 4.2.4.A/2- 11-1-2012-0001
TÁMOP
K104984
OTKA
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9.

001-es BibID:BIBFORM054229
035-os BibID:(PMID)25112418 (WOS)000344875700028 (Scopus)84939883199
Első szerző:Juhász Tamás (biológus, orvosbiológus)
Cím:Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP) Signalling Enhances Osteogenesis in UMR-106 Cell Line / Tamás Juhász, Csaba Matta, Éva Katona, Csilla Somogyi, Roland Takács, Tibor Hajdú, Solveig Lind Helgadottir, János Fodor, László Csernoch, Gábor Tóth, Éva Bakó, Dóra Reglődi, Andrea Tamás, Róza Zákány
Dátum:2014
ISSN:0895-8696
Megjegyzések:Presence of the pituitary adenylate cyclase-activating polypeptide (PACAP) signalling has been proved in various peripheral tissues. PACAP can activate protein kinase A (PKA) signalling via binding to pituitary adenylate cyclase-activating polypeptide type I receptor (PAC1), vasoactive intestinal polypeptide receptor (VPAC) 1 or VPAC2 receptor. Since little is known about the role of this regulatory mechanism in bone formation, we aimed to investigate the effect of PACAP on osteogenesis of UMR-106 cells. PACAP 1-38 as an agonist and PACAP 6-38 as an antagonist of PAC1 were added to the culture medium. Surprisingly, both substances enhanced protein expressions of collagen type I, osterix and alkaline phosphatase, along with higher cell proliferation rate and an augmented mineralisation. Although expression of PKA was elevated, no alterations were detected in the expression, phosphorylation and nuclear presence of CREB, but increased nuclear appearance of Runx2, the key transcription factor of osteoblast differentiation, was shown. Both PACAPs increased the expressions of bone morphogenetic proteins (BMPs) 2, 4, 6, 7 and Smad1 proteins, as well as that of Sonic hedgehog, PATCH1 and Gli1. Data of our experiments indicate that activation of PACAP pathway enhances bone formation of UMR-106 cells and PKA, BMP and Hedgehog signalling pathways became activated. We also found that PACAP 6-38 did not act as an antagonist of PACAP signalling in UMR-106 cells.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Megjelenés:Journal of Molecular Neuroscience. - 54 : 3 (2014), p. 555-573. -
További szerzők:Matta Csaba (1980-) (molekuláris biológus, genetikus, angol szakfordító) Katona Éva (1986-) (molekuláris biológus) Somogyi Csilla (1983-) (biológus, angol-magyar szakfordító) Takács Roland Ádám (1985-) (molekuláris biológus, biokémikus) Hajdú Tibor (1988-) (általános orvos) Helgadottir, Solveig Lind Fodor János (1973-) (élettanász, biotechnológus) Csernoch László (1961-) (élettanász) Tóth Gábor Bakó Éva (1958-) (biokémikus) Reglődi Dóra (Idegtudományok) Tamás Andrea (Idegtudomány) (Pécs) Zákány Róza (1963-) (anatómus-, kötőszövetbiológus)
Pályázati támogatás:K 104984
OTKA
CNK80709
OTKA
TÁMOP-4.2.1.B-10/2KONV-2010-002
TÁMOP
TÁMOP-4.2.2.A-11/1/KONV-2012-0053
TÁMOP
TÁMOP-4.2.2.A-11/1/KONV-2012-0024
TÁMOP
TÁMOP-4.2.4.A/2-11-1-2012-0001
TÁMOP
Mecenatura Mec-9/2011
Egyéb
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10.

001-es BibID:BIBFORM042639
Első szerző:Koppán Miklós
Cím:Correlation Between Oocyte Number and Follicular Fluid Concentration of Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP) in Women After Superovulation Treatment / M. Koppan, A. Varnagy, D. Reglodi, R. Brubel, J. Nemeth, A. Tamas, L. Mark, J. Bodis
Dátum:2012
Megjegyzések:Follicular growth, ovulation, and luteinization areinfluenced by interactions of peptide and steroid hormonesignalingcascades in the ovary. Pituitary adenylate cyclaseactivatingpolypeptide (PACAP) plays an important role in theregulation of several endocrine processes and is present inovarian follicular fluid (FF). However, little is known aboutPACAP in FF with regard to maturation, ovulation, fertilization,and successful pregnancy. The aim of this pilot study wasto investigate whether there is a correlation between PACAPconcentration in FF and ovarian response to superovulationtreatment in infertile women, performed in volunteers (n0132; aged between 20 and 35). After treatment, the numberof harvested oocytes was recorded and PACAP immunoreactivityin FF was measured by radioimmunoassay. All thecorresponding PACAP concentrations were below 290 fmol/ml in cases when the number of harvested oocytes exceeded 14per patient, while in all cases above 290 fmol/ml, the number ofoocytes was below 14. Using these cutoff values, we determinedthree study groups: high-PACAP concentration, highoocytenumber, and low-PACAP concentration?low-oocytenumber groups. Median values of PACAP concentration inthese groups were 411.2, 106.5, and 101.0 fmol/ml, respectively,while themedian values of harvested oocyteswere 5.5, 19.0,and 5.0, respectively. Differences were significant, indicating acorrelation between concentration of PACAP in FF and thenumber of recruited oocytes. Higher concentrations of PACAPin FF might be associated with lower number of developingoocytes, while low concentrations of PACAP might correlatewith a markedly higher number of ova retrieved, thus predictinga higher chance for ovarian hyperstimulation. Our presentstudy is among the first few human clinical studies with directconclusions drawn for possible clinical impact of PACAP.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
RIA
Follicular fluid
Superovulation treatment
Human
Neuropeptide
Megjelenés:Journal of Molecular Neuroscience. - 48 (2012), p. 617-622. -
További szerzők:Várnagy A. Reglődi Dóra (Idegtudományok) Brubel Réka Németh József (1954-) (vegyész, analitikus) Tamás A. (Pécs) Mark L. Bodis József (Pécs)
Pályázati támogatás:OTKA-72592
OTKA
Internet cím:DOI
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11.

001-es BibID:BIBFORM076127
Első szerző:László Eszter
Cím:Protective Effect of PACAP on Ischemia/Reperfusion-Induced Kidney Injury of Male and Female Rats : gender Differences / Eszter Laszlo, Tamas Juhasz, Adam Varga, Bernadett Czibere, Krisztina Kovacs, Peter Degrell, Gabriella Horvath, Gabor Jancso, Peter Szakaly, Andrea Tamas, Dora Reglodi
Dátum:2019
ISSN:0895-8696
Megjegyzések:Pituitary adenylate cyclase-activating polypeptide (PACAP) is a neuropeptide that exerts general cytoprotective effects, including protection in different kidney disorders. The aim of our study was to investigate the ischemia/reperfusion-induced kidney injury of male and female rats to confirm the protective effects of PACAP in the kidney and to reveal possible gender differences.Male and female Wistar rats underwent unilateral renal artery clamping followed by 24-h, 48-h, or 14-day reperfusion. PACAP was administered intravenously before arterial clamping in half of the rats. Tubular damage, cytokine expression pattern, oxidative stress marker, antioxidative status and signaling pathways were evaluated using histology, immunohistology, cytokine array, PCR, and Western blot. Tubular damage was significantly less severe in the PACAP-treated male and female rats compared to controls. Results of female animals were significantly better in both treated and untreated groups. Cytokine expression, oxidative stress marker and antioxidative status confirmed the histological results. We also revealed that PACAP counteracted the decreased PKA phosphorylation, influenced the expression of BMP2 and BMP4, and increased the expression of the protein Smad1.We conclude that PACAP is protective in ischemia/reperfusion-induced kidney injury in both sexes, but females had markedly less pronounced injury after ischemia/reperfusion, possibly also involving further protective factors, the investigation of which could have future therapeutic value in treating ischemic kidney injuries.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Journal Of Molecular Neuroscience. - 68 : 3 (2019), p. 408-419. -
További szerzők:Juhász Tamás (1976-) (biológus, orvosbiológus) Varga Ádám Czibere Bernadett Kovács Krisztina Degrell Péter Horváth Gabriella Jancsó Gábor Szakály Péter Tamás Andrea (Idegtudomány) (Pécs) Reglődi Dóra (Idegtudományok)
Pályázati támogatás:EFOP-3.6.2-16-2017-00008
EFOP
NKFIH119759
NKFIH
GINOP-2.3.2-15-2016-00050
GINOP
EFOP-3.6.1.-16-2016-00004
EFOP
EFOP-3.6.3-VEKOP-16-2017-00009
EFOP
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DOI
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12.

001-es BibID:BIBFORM042646
Első szerző:Nedvig Klára
Cím:Changes of PACAP immunoreactivities and cytokine levels after PACAP-38 containing intestinal preservation and autotransplantation / Klara Nedvig, Gyorgy Weber, Jozsef Nemeth, Krisztina Kovacs, Dora Reglodi, Agnes Kemeny, Andrea Ferencz
Dátum:2012
Megjegyzések:Small bowel is one of the most sensitive organs toischemia?reperfusion injury, which is a significant problemduring transplantation. Pituitary adenylate cyclaseactivatingpolypeptide (PACAP) has cytoprotective effect inischemic injuries of various tissues. The aim of our study wasto measure changes of PACAP-38 and PACAP-27 immunoreactivitiesand cytokine levels in intestinal grafts stored inPACAP-38-containing preservation solution. Small bowelautotransplantation was performed on maleWistar rats. Graftswere stored in University ofWisconsin (UW) solution at 4 ?Cfor 1 h (group (G)I), for 3 h (GII), and for 6 h (GIII) and inPACAP-38-containing UW solution for 1 h (GIV), for 3 h(GV), and for 6 h (GVI).After preservation, performing vesselanastomosis reperfusion began, which lasted 3 h in eachgroup. Tissue biopsies were collected after laparotomy(control) and at the end of the reperfusion periods. IntestinalPACAP-38 and PACAP-27 immunoreactivities were measuredby radioimmunoassay. To measure cytokines from tissuehomogenates, we used rat cytokine array and LuminexMultiplex Immunoassay. Levels of PACAP-38 and PACAP-27 immunoreactivity decreased after 1 and 3 h preservationcompared to control levels. This decrease was significantfollowing 6 h cold storage (p<0.05). Values remained significantlyhigher in grafts stored in PACAP-38-containing UW.Cytokine array revealed that expression of the soluble intercellularadhesion molecule-1 (CD54) and L-selectin (CD62L/LECAM-1) was increased in GIII. Both 6 h cold storage inPACAP-38-containing UW solution and 3 h reperfusioncaused strong reduction in these cytokines activation in GVI.RANTES (CCL5) levels were increased in all groups. Strongactivation of the tissue inhibitor of metalloproteinase-1 was inGIII. However, PACAP-38-containing cold storage could decreaseits activation in GVI. Furthermore, strong activation ofthe tissue inhibitor of metalloproteinase-1 was detected in 6 hpreserved grafts without PACAP-38 (GIII). PACAP-38-containing cold storage could decrease its activation in GVI.Our present study showed that PACAP-38 and PACAP-27immunoreactivities decreased in a time-dependent mannerduring intestinal cold preservation, which could be amelioratedby administration of exogenous PACAP-38 to the preservationsolution. Moreover, PACAP-38 could attenuate tissuecold ischemic injury-induced changes in cytokine expression.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Small bowel
Transplantation
PACAP-38
PACAP-27
Cytokine
Megjelenés:Journal of Molecular Neuroscience. - 48 : 3 (2012), p. 788-794. -
További szerzők:Wéber György Németh József (1954-) (vegyész, analitikus) Kovács Krisztina (Pécs) Reglődi Dóra (Idegtudományok) Kemény Ágnes Ferencz Andrea
Pályázati támogatás:OTKA-77474
OTKA
Internet cím:DOI
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