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001-es BibID:BIBFORM092901
035-os BibID:(WoS)000518794300002 (Scopus)85076683169
Első szerző:De Leoz, Maria Lorna A.
Cím:NIST Interlaboratory Study on Glycosylation Analysis of Monoclonal Antibodies: Comparison of Results from Diverse Analytical Methods / Maria Lorna A. De Leoz, David L. Duewer, Adam Fung, Lily Liu, Hoi Kei Yau, Oscar Potter, Gregory O. Staples, Kenichiro Furuki, Ruth Frenkel, Yunli Hu, Zoran Sosic, Peiqing Zhang, Friedrich Altmann, Clemens Grunwald-Grube, Chun Shao, Joseph Zaia, Waltraud Evers, Stuart Pengelley, Detlev Suckau, Anja Wiechmann, Anja Resemann, Wolfgang Jabs, Alain Beck, John W. Froehlich, Chuncui Huang, Yan Li, Yaming Liu, Shiwei Sun, Yaojun Wang, Youngsuk Seo, Hyun Joo An, Niels-Christian Reichardt, Juan Echevarria Ruiz, Stephanie Archer-Hartmann, Parastoo Azadi, Len Bell, Zsuzsanna Lakos, Yanming An, John F. Cipollo, Maja Pucic-Bakovic, Jerko Štambuk, Gordan Lauc, Xu Li, Peng George Wang, Andreas Bock, René Hennig, Erdmann Rapp, Marybeth Creskey, Terry D. Cyr, Miyako Nakano, Taiki Sugiyama, Pui-King Amy Leung, Paweł Link-Lenczowski, Jolanta Jaworek, Shuang Yang, Hui Zhang, Tim Kelly, Song Klapoetke, Rui Cao, Jin Young Kim, Hyun Kyoung Lee, Ju Yeon Lee, Jong Shin Yoo, Sa-Rang Kim, Soo-Kyung Suh, Noortje de Haan, David Falck, Guinevere S. M. Lageveen-Kammeijer, Manfred Wuhrer, Robert J. Emery, Radoslaw P. Kozak, Li Phing Liew, Louise Royle, Paulina A. Urbanowicz, Nicolle H. Packer, Xiaomin Song, Arun Everest-Dass, Erika Lattová, Samanta Cajic, Kathirvel Alagesan, Daniel Kolarich, Toyin Kasali, Viv Lindo, Yuetian Chen, Kudrat Goswami, Brian Gau, Ravi Amunugama, Richard Jones, Corné J. M. Stroop, Koichi Kato, Hirokazu Yagi, Sachiko Kondo, C. T. Yuen, Akira Harazono, Xiaofeng Shi, Paula E. Magnelli, Brian T. Kasper, Lara Mahal, David J. Harvey, Roisin O'Flaherty, Pauline M. Rudd, Radka Saldova, Elizabeth S. Hecht, David C. Muddiman, Jichao Kang, Prachi Bhoskar, Daniele Menard, Andrew Saati, Christine Merle, Steven Mast, Sam Tep, Jennie Truong, Takashi Nishikaze, Sadanori Sekiya, Aaron Shafer, Sohei Funaoka, Masaaki Toyoda, Peter de Vreugd, Cassie Caron, Pralima Pradhan, Niclas Chiang Tan, Yehia Mechref, Sachin Patil, Jeffrey S. Rohrer, Ranjan Chakrabarti, Disha Dadke, Mohammedazam Lahori, Chunxia Zou, Christopher Cairo, Béla Reiz, Randy M. Whittal, Carlito B. Lebrilla, Lauren Wu, Andras Guttman, Marton Szigeti, Benjamin G. Kremkow, Kelvin H. Lee, Carina Sihlbom, Barbara Adamczyk, Chunsheng Jin, Niclas G. Karlsson, Jessica Örnros, Göran Larson, Jonas Nilsson, Bernd Meyer, Alena Wiegandt, Emy Komatsu, Helene Perreault, Edward D. Bodnar, Nassur Said, Yannis-Nicolas Francois, Emmanuelle Leize-Wagner, Sandra Maier, Anne Zeck, Albert J. R. Heck, Yang Yang, Rob Haselberg, Ying Qing Yu, William Alley, Joseph W. Leone, Hua Yuan, Stephen E. Stein
Dátum:2020
ISSN:1535-9476
Megjegyzések:Glycosylation is a topic of intense current interest in the development of biopharmaceuticals because it is related to drug safety and efficacy. This work describes results of an interlaboratory study on the glycosylation of the Primary Sample (PS) of NISTmAb, a monoclonal antibody reference material. Seventy-six laboratories from industry, university, research, government, and hospital sectors in Europe, North America, Asia, and Australia submitted a total of 103 reports on glycan distributions. The principal objective of this study was to report and compare results for the full range of analytical methods presently used in the glycosylation analysis of mAbs. Therefore, participation was unrestricted, with laboratories choosing their own measurement techniques. Protein glycosylation was determined in various ways, including at the level of intact mAb, protein fragments, glycopeptides, or released glycans, using a wide variety of methods for derivatization, separation, identification, and quantification. Consequently, the diversity of results was enormous, with the number of glycan compositions identified by each laboratory ranging from 4 to 48. In total, one hundred sixteen glycan compositions were reported, of which 57 compositions could be assigned consensus abundance values. These consensus medians provide community-derived values for NISTmAb PS. Agreement with the consensus medians did not depend on the specific method or laboratory type. The study provides a view of the current state-of-the-art for biologic glycosylation measurement and suggests a clear need for harmonization of glycosylation analysis methods.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Megjelenés:Molecular & Cellular Proteomics. - 19 : 1 (2020), p. 11-30. -
További szerzők:Duewer, David L. Fung, Adam Liu, Lily Yau, Hoi Kei Potter, Oscar Staples, Gregory O. Furuki, Kenichiro Frenkel, Ruth Hu, Yunli Sosic, Zoran Zhang, Peiqing Altmann, Friedrich Grunwald-Grube, Clemens Shao, Chun Zaia, Joseph Evers, Waltraud Pengelley, Stuart Suckau, Detlev Wiechmann, Anja Resemann, Anja Jabs, Wolfgang Beck, Alain Froehlich, John W. Huang, Chuncui Li, Yan Liu, Yaming Sun, Shiwei Wang, Yaojun Seo, Youngsuk An, Hyun Joo Reichardt, Niels-Christian Ruiz, Juan Echevarria Archer-Hartmann, Stephanie Azadi, Parastoo Bell, Len Lakos Zsuzsanna An, Yanming Cipollo, John F. Pucic-Bakovic, Maja Stambuk, Jerko Lauc, Gordan Li, Xu Wang, Peng George Bock, Andreas Hennig, René Rapp, Erdmann Creskey, Marybeth Cyr, Terry D. Nakano, Miyako Sugiyama, Taiki Leung, Pui-King Link-Lenczowski, Paweł Jaworek, Jolanta Yang, Shuang Zhang, Hui Kelly, Tim Klapoetke, Song Cao, Rui Kim, Jiyoung Lee, Hyun Kyoung Lee, Ju Yeon Yoo, Jong Shin Kim, Sa-Rang Suh, Soo-Kyung de Haan, Noortje Falck, David Lageveen-Kammeijer, Guinevere S. M. Wuhrer, Manfred Emery, Robert J. Kozak, Radoslaw P. Liew, Li Phing Royle, Louise Urbanowicz, Paulina A. Packer, Nicolle Song, Xiaomin Everest-Dass, Arun Lattová, Erika Cajic, Samanta Alagesan, Kathirvel Kolarich, Daniel Kasali, Toyin Lindo, Viv Chen, Yuetian Goswami, Kudrat Gau, Brian Amunugama, Ravi Jones, Richard Stroop, Corné J. M. Kato, Koichi Yagi, Hirokazu Kondo, Sachiko Yuen, C. T. Harazono, Akira Shi, Xiaofeng Magnelli, Paula E. Kasper, Brian T. Mahal, Lara Harvey, David J. O'Flaherty, Roisin Rudd, Pauline M. Saldova, Radka Hecht, Elizabeth S. Muddiman, David C. Kang, Jichao Bhoskar, Prachi Menard, Daniele Saati, Andrew Merle, Christine Mast, Steven Tep, Sam Truong, Jennie Nishikaze, Takashi Sekiya, Sadanori Shafer, Aaron Funaoka, Sohei Toyoda, Masaaki de Vreugd, Peter Caron, Cassie Pradhan, Pralima Tan, Niclas Chiang Mechref, Yehia Patil, Sachin Rohrer, Jeffrey S. Chakrabarti, Ranjan Dadke, Disha Lahori, Mohammedazam Zou, Chunxia Cairo, Christopher Reiz, Béla Whittal, Randy M. Lebrilla, Carlito B. Wu, Lauren Guttman András (1954-) (vegyészmérnök) Szigeti Márton (1986-) (környezetmérnök) Kremkow, Benjamin G. Lee, Kelvin H. Sihlbom, Carina Adamczyk, Barbara Jin, Chunsheng Karlsson, Niclas G. Örnros, Jessica Larson, Göran Nilsson, Jonas Meyer, Bernd Wiegandt, Alena Komatsu, Emy Perreault, Helene Bodnar, Edward D. Said, Nassur Francois, Yannis-Nicolas Leize-Wagner, Emmanuelle Maier, Sandra Zeck, Anne Heck, Albert J. R. Yang, Yang Haselberg, Rob Yu, Ying Qing Alley, William Leone, Joseph W. Yuan, Hua Stein, Stephen E.
Pályázati támogatás:NKFIH-K 116263
NKFIH
GINOP-2.3.2- 15-2016-00017
GINOP
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Intézményi repozitóriumban (DEA) tárolt változat
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2.

001-es BibID:BIBFORM023718
035-os BibID:(cikkazonosító)M111.010298 (WoS)000298290300017 (Scopus)83055168546
Első szerző:Guergova-Kuras, Mariana
Cím:Discovery of lung cancer biomarkers by profiling the plasma proteome with monoclonal antibody libraries / Mariana Guergova-Kuras, István Kurucz, William Hempel, Nadege Tardieu, János Kádas, Carole Malderez-Bloes, Anne Jullien, Yann Kieffer, Marina Hincapie, András Guttman, Eszter Csánky, Balázs Dezső, Barry L. Karger, László Takács
Dátum:2011
ISSN:1535-9476 1535-9484
Megjegyzések:A challenge in the treatment of lung cancer is the lack of early diagnostics. Here, we describe the application of monoclonal antibody (mAb) proteomics for discovery of a panel of biomarkers for early detection (stage I) of non small cell lung cancer (NSCLC). We produced large monoclonal antibody libraries directed against the natural form of protein antigens present in the plasma of NSCLC patients. Plasma biomarkers associated with the presence of lung cancer were detected via high throughput ELISA. Differential profiling of plasma proteomes of four clinical cohorts, totalling 301 patients with lung cancer and 235 healthy controls, identified 13 lung cancer associated (p<0.05) monoclonal antibodies. The mAbs recognize five different cognate proteins identified using immunoprecipitation followed by mass spectrometry. Four of the five antigens were present in non-small cell lung cancer cells in-situ. The approach is capable of generating independent antibodies against different epitopes of the same proteins, allowing fast translation to multiplexed sandwich assays. Based on these results, we have verified in two independent clinical collections a panel of five biomarkers for classifying patient disease status with a diagnostics performance of 77% sensitivity and 87% specificity. Combining CYFRA, an established cancer marker, with the panel resulted in a performance of 83 % sensitivity at 95 % specificity for stage I NSCLC.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Megjelenés:Molecular and Cellular Proteomics. - 10 : 12 (2011), p. 1-14. -
További szerzők:Kurucz István Hempel, William Tardieu, Nadège Kádas János (1976-) (molekuláris biológus, biokémikus, kertészmérnök) Malderez-Bloes, Carole Jullien, Anne Kieffer, Yann Hincapie, Marina Guttman András (1954-) (vegyészmérnök) Csánky Eszter (1959-) (tüdőgyógyász, klinikai immunológus, allergológus) Karger, Barry Takács László (1955-) Dezső Balázs (1951-) (pathológus)
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
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3.

001-es BibID:BIBFORM009630
Első szerző:Hádáné Birkó Zsuzsanna (molekuláris genetikus)
Cím:Lack of A-factor production induces the expression of nutrient scavenging and stress-related proteins in Streptomyces griseus / Birkó Zsuzsanna, Swiatek Magdalena, Szájli Emília, Medzihradszky F. Katalin, Vijgenboom Erik, Penyige András, Keserű Judit, van Wezel Gilles P., Biró Sándor
Dátum:2009
ISSN:1535-9476 (Print)
Megjegyzések:The small gamma-butyrolactone A-factor is an important autoregulatory signaling molecule for the soil-inhabiting streptomycetes. Starvation is a major trigger for development,and nutrients are provided by degradation of the vegetative mycelium via a process of programmed cell death, reusing proteins, nucleic acids, and cell wall material. The A-factor regulon includes many extracellular hydrolases. Here we show via proteomics analysis that many nutrientscavenging and stress-related proteins were overexpressed in an A-factor non-producing mutant of Streptomyces griseus B-2682. Transcript analysis showed that this is primarily due to differential transcription of the target genes during early development. The targets include proteins relating to nutrient stress and environmental stress and an orthologue of the Bacillus sporulation control protein Spo0M. The enhanced expression of these proteins underlines the stress that is generated by the absence of A-factor. Wild-type developmental gene expression was restored to the A-factor non-producing mutant by the signaling protein Factor C in line with our earlier observation that Factor C triggers A-factor production.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
C-faktor
Streptomyces griseus
proteomika
Megjelenés:Molecular and Cellular Proteomics : MCP - 8 : 10 (2009), p. 2396-2403. -
További szerzők:Swiatek, Magdalena Szájli Emília Medzihradszky-Fölkl Katalin Vijgenboom, Erik Penyige András (1954-) (molekuláris genetikus) Keserű Judit (1976-) (molekuláris genetikus) Wezel, Gilles P., van Biró Sándor (1949-) (molekuláris genetikus)
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elektronikus változat
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4.

001-es BibID:BIBFORM001540
Első szerző:Hádáné Birkó Zsuzsanna (molekuláris genetikus)
Cím:The secreted signaling protein factor C triggers the A-factor response regulon in streptomyces griseus : overlapping signaling routes / Birkó Z., Bialek S., Buzás K., Szájli E., Traag B. A., Medzihradszky K. F., Rigali S., Vijgenboom E., Penyige A., Kele Z., van Wezel G. P., Bíró S.
Dátum:2007
Megjegyzések:Members of the prokaryotic genus Streptomyces produce over 60% of all known antibiotics and a wide range of industrial enzymes. A leading theme in microbiology is which signals are received and transmitted by these organisms to trigger the onset of morphological differentiation and antibiotic production. The small -butyrolactone A-factor is an important autoregulatory signaling molecule in streptomycetes, and A-factor mutants are blocked in development and antibiotic production. In this study we showed that heterologous expression of the 324-amino acid secreted regulatory protein Factor C resulted in restoration of development and enhanced antibiotic production of an A-factor-deficient bald mutant of Streptomyces griseus, although the parental strain lacks an facC gene. Proteome analysis showed that in the facC transformant the production of several secreted proteins that belong to the A-factor regulon was restored. HPLC-MS/MS analysis indicated that this was due to restoration of A-factor production to wild-type levels in the transformant. This indicates a connection between two highly divergent types of signaling molecules and possible interplay between their regulatory networks.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
Streptomyces szignalizáció
Megjelenés:Molecular and Cellular Proteomics : MCP - 6 : 7 (2007), p. 1248-1256. -
További szerzők:Bialek, Sylwia Buzás Krisztina Szájli Emília Traag, Bjorn A. Medzihradszky-Fölkl Katalin Rigali, Sebastien Vijgenboom, Erik Kele Zoltán Wezel, Gilles P., van Biró Sándor (1949-) (molekuláris genetikus) Penyige András (1954-) (molekuláris genetikus)
Internet cím:elektronikus változat
DOI
elektronikus változat
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5.

001-es BibID:BIBFORM111963
035-os BibID:(cikkazonosító)100580 (Scopus)85165521769
Első szerző:Lázár József
Cím:Large-scale plasma proteome epitome profiling is an efficient tool for the discovery of cancer biomarkers / Lazar Jozsef, Antal-Szalmas Peter, Kurucz Istvan, Ferenczi Annamaria, Jozsi Mihaly, Tornyi Ilona, Muller Monika, Fekete Janos Tibor, Lamont John, FitzGerald Peter, Gall-Debreceni Anna, Kadas Janos, Vida Andras, Tardieu Nadege, Kieffer Yann, Jullien Anne, Guergova-Kuras Mariana, Hempel William, Kovacs Andras, Kardos Tamas, Bittner Nora, Csanky Eszter, Szilasi Maria, Losonczy Gyorgy, Szondy Klara, Galffy Gabriella, Csada Edit, Szalontai Klara, Somfay Attila, Malka David, Cottu Paul, Bogos Krisztina, Takacs Laszlo
Dátum:2023
ISSN:1535-9476
Megjegyzések:Current proteomic technologies focus on the quantification of protein levels, while little effort is dedicated to the development of systems approaches to simultaneously monitor proteome variability and abundance. Protein variants may display different immunogenic epitopes detectable by monoclonal antibodies. Epitope variability results from alternative splicing, posttranslational modifications, processing, degradation, and complex formation and possess dynamically changing availability of interacting surface structures frequently serve as reachable epitopes, and often carry different functions. Thus, it is highly likely, that the presence of some of the accessible epitopes correlate with function under physiological and pathological conditions. To enable the exploration of the impact of protein variation on the immunogenic epitome first; here, we present a robust and analytically validated protein epitome profiling (PEP) technology for characterizing immunogenic epitopes of the plasma. To this end we prepared mAb libraries directed against the normalized human plasma proteome as a complex natural immunogen. Resulting hybridoma supernatants were selected for mAb production and the corresponding hybridomas were cloned. Monoclonal antibodies react with single epitopes, thus profiling with the libraries is expected to profile many epitopes which we define by the mimotopes, as we present here. Screening blood plasma samples from control subjects (n = 558) and cancer patients (n = 598) for merely 69 native epitopes displayed by 20 abundant plasma proteins resulted in distinct cancer-specific epitope panels that showed high accuracy (AUC 0.826?0.966) and specificity for lung, breast, and colon cancer. Deeper profiling (?290 epitopes of approximately 100 proteins) showed unexpected granularity of the epitope-level expression data and detected neutral and lung-cancer associated epitopes of individual proteins. Biomarker epitope panels selected from a pool of 21 epitopes of 12 proteins were validated in independent clinical cohorts. The results demonstrate the value of PEP as a rich and thus far unexplored source of protein biomarkers with diagnostic potential.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Megjelenés:Molecular & Cellular Proteomics. - 22 : 7 (2023), p. 1-18. -
További szerzők:Antal-Szalmás Péter (1968-) (laboratóriumi szakorvos) Kurucz István Ferenczi Annamária (1986-) (molekuláris biológus, mikrobiológus) Józsi Mihály Tornyi Ilona (1982-) (molekuláris biológus) Müller Mónika Fekete János Tibor Lamont, John FitzGerald, Peter Gall-Debreceni Anna Kádas János (1976-) (molekuláris biológus, biokémikus, kertészmérnök) Vida András (1979-) (molekuláris biológus, genetikus) Tardieu, Nadège Kieffer, Yann Jullien, Anne Guergova-Kuras, Mariana Hempel, William Kovács András László (1969-) (biológus, biológia-kémia tanár) Kardos Tamás (1980-) (pulmonológus, klinikai onkológus) Bittner Nóra (1963-) (orvos) Csánky Eszter (1959-) (tüdőgyógyász, klinikai immunológus, allergológus) Szilasi Mária (1953-) (tüdőgyógyász, klinikai immunológus, allergológus, belgyógyász) Losonczy György Szondy Klára Gálffy Gabriella Csada Edit Szalontai Klára Somfay Attila Malka, David Cottu, Paul Bogos Krisztina Takács László (1955-) (orvos)
Pályázati támogatás:TECH-09-A1-2009-0113; mAbCHIC
Egyéb
GOP-1.2.1-09-2010-0019
Egyéb
Internet cím:Szerző által megadott URL
DOI
Intézményi repozitóriumban (DEA) tárolt változat
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6.

001-es BibID:BIBFORM083100
035-os BibID:(WoS)000501288700014 (Scopus)85075963083
Első szerző:Szigeti Márton (környezetmérnök)
Cím:Sample Preparation Scale-Up for Deep N-glycomic Analysis of Human Serum by Capillary Electrophoresis and CE-ESI-MS / Szigeti Marton, Guttman Andras
Dátum:2019
ISSN:1535-9476
Megjegyzések:We introduce an efficient sample preparation workflow to facilitate deep N-glycomics analysis of the human serum by capillary electrophoresis with laser induced fluorescence (CE-LIF) detection and to accommodate the higher sample concentration requirement of electrospray ionization mass spectrometry connected to capillary electrophoresis (CE-ESI-MS). A novel, temperature gradient denaturing protocol was applied on amine functionalized magnetic bead partitioned glycoproteins to circumvent the otherwise prevalent precipitation issue. During this process, the free sugar content of the serum was significantly decreased as well, accommodating enhanced PNGase F mediated release of the N-linked carbohydrates. The liberated oligosaccharides were tagged with aminopyrene- trisulfonate, utilizing a modified evaporative labeling protocol. Processing the samples with this new workflow enabled deep CE-LIF analysis of the human serum N-glycome and provided the appropriate amount of material for CE-ESI-MS analysis in negative ionization mode. Molecular & Cellular Proteomics 18: 2524?2531, 2019. DOI: 10.1074/mcp.TIR119.001669.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Megjelenés:Molecular & Cellular Proteomics. - 18 : 12 (2019), p. 2524-2531. -
További szerzők:Guttman András (1954-) (vegyészmérnök)
Pályázati támogatás:K 116263
NKFIH
BIONANO_GINOP-2.3.2-15-2016-00017
GINOP
Internet cím:Szerző által megadott URL
DOI
Intézményi repozitóriumban (DEA) tárolt változat
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