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001-es BibID:	BIBFORM057465
Első szerző:	Kappelmayer János (laboratóriumi szakorvos)
Cím:	Thrombocyta/megakaryocyta markerek áramlási citometriai alkalmazhatósága / Kappelmayer János, Kiss Csongor, Udvardy Miklós, Csáthy László, Kiss Flóra, Simon Ágnes, Hevessy Zsuzsanna
Dátum:	2013
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idézhető absztrakt
Megjelenés:	Hematológia-Transzfuziológia. Supplementum. - 46 : Suppl. 1 (2013), p. 73. -
További szerzők:	Kiss Csongor (1956-) (hematológus, onkológus) Udvardy Miklós (1947-) (belgyógyász, haematológus) Csáthy László (1979-) (laboratóriumi szakorvos) Kiss Flóra (1980-) (bőrgyógyász) Simon Ágnes (1969-) (laboratóriumi szakorvos) Hevessy Zsuzsanna (1966-) (laboratóriumi szakorvos)
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM034578
Első szerző:	Kappelmayer János (laboratóriumi szakorvos)
Cím:	Coagulation factor XIII-A. A flow cytometric intracellular marker in the classification of acute myeloid leukemias / Kappelmayer, J., Simon, A., Katona, E., Szanto, A., Nagy, L., Kiss, A., Kiss, C., Muszbek, L.
Dátum:	2005
ISSN:	0340-6245
Megjegyzések:	The association of coagulation factors with leukocytes have been demonstrated in several previous studies. This study was designed to study the sensitivity and specificity of factor XIII subunit A (FXIII-A) labelling in cultured myeloblastic and monoblastic cell lines and to investigate the intracytoplasmic expression of FXIII-A in de novo acute myeloid leukemia (AML) samples. Myeloblastic and a monoblastic cell lines were cultured and investigated for lineage specific maturation markers and FXIII-A expression. Furthermore, FXIII-A expression was investigated in 12 normal samples (7 bone marrow and 5 peripheral blood), 86 de novo AML samples and 6 chronic myelomonocytic leukemia (CMML) samples. In the monoblastic MonoMac6 cell line the appearance of FXIII-A preceded that of CD14 while it remained negative in the myeloblastic PLB-985 cell line throughout its maturation period. Among the AML samples the average frequency of FXIII-A positive cells in myeloblastic leukemia samples was below 10%, while in M4 and M5AML samples it was above 50% and was significantly higher than the generally used CD14 marker (p < 0.0001). In the AML M4 and M5 cases, FXIII-A proved sensitive for the identification of monoblasts. FXIII-A can be considered as a reliable intracytoplasmic marker for the monocytic and megakaryocytic series and its presence is highly predictive for mono- and megakaryocytic AML and for CMML.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	Thrombosis And Haemostasis. - 94 : 2 (2005), p. 454-459. -
További szerzők:	Simon Ágnes (1969-) (laboratóriumi szakorvos) Katona Éva (1961-) (klinikai biokémikus) Szántó Attila (1976-) (orvos, biokémikus) Nagy László (1966-) (molekuláris sejtbiológus, biokémikus) Kiss Attila (1942-) (belgyógyász, haematológus) Kiss Csongor (1956-) (hematológus, onkológus) Muszbek László (1942-) (haematológus, kutató orvos)
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM005605
Első szerző:	Kiss Flóra (bőrgyógyász)
Cím:	A coagulation factor becomes useful in the study of acute leukemias : studies with blood coagulation factor XIII / Kiss F., Simon Á., Csáthy L., Hevessy Z., Katona É., Kiss C., Kappelmayer J.
Dátum:	2008
Megjegyzések:	The intracellular form of the coagulation factor XIII has previously been identified by immunomorphological techniques using polyclonal antibodies. In these studies, only the A subunit (FXIII-A) was detectable in megakaryocytes/platelets and in monocytes/ macrophages. We developed several novel monoclonal antibody clones directed to both subunits (FXIII-A and FXIII-B) and investigated their appearance in normal and leukemic cells. By using 3- and 4-color flow cytometry FXIII expression was investigated in normal peripheral blood and bone marrow samples and in acute myeloblastic (AML) and lymphoblastic (ALL) leukemia cases. Samples were studied by Western blotting and confocal laser scanning microscopy. With a previously published ELISA assay applying two monoclonal antibodies directed to different epitopes in FXIII-A, we were able to measure the intracytoplasmic content of FXIII-A in normal cells and leukemic blasts. FXIII-A was detectable in normal peripheral blood monocytes and in large quantities in platelets, but both cell types were negative for FXIII-B. There was no surface staining for FXIII-A, it only appeared intracellularly. In samples derived from patients with AML M4 and M5, FXIII-A sensitively identified blast cells. Although normal lymphocytes do not express FXIII-A, 40% of ALL cases showed significant FXIII-A expression as determined by flow cytometry. FXIII-A positivity of lymphoblasts was verified by Western blotting, ELISA, and confocal laser scanning microscopy cytometry. These data provide evidence that FXIII-A is a sufficiently sensitive marker in differentiating myeloblasts and monoblasts and is suitable for identifying leukemia-associated phenotypes in ALL.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban factor XIII flow cytometry acute leukemia phenotype
Megjelenés:	Cytometry. Part A. - 73A : 3 (2008), p. 194-201. -
További szerzők:	Simon Ágnes (1969-) (laboratóriumi szakorvos) Csáthy László (1979-) (laboratóriumi szakorvos) Hevessy Zsuzsanna (1966-) (laboratóriumi szakorvos) Katona Éva (1961-) (klinikai biokémikus) Kiss Csongor (1956-) (hematológus, onkológus) Kappelmayer János (1960-) (laboratóriumi szakorvos)
Internet cím:	DOI elektronikus változat
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