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1.

001-es BibID:BIBFORM001292
Első szerző:Palatka Károly (belgyógyász, gasztroenterológus)
Cím:Changes in the expression and distribution of the inducible and endothelial nitric oxide synthase in mucosal biopsy specimens of inflammatory bowel disease / Károly Palatka, Zoltán Serfőző, Zoltán Veréb, Zoltán Hargitay, Bea Lontay, Ferenc Erdődi, Gáspár Bánfalvi, Zoltán Nemes, Miklós Udvardy, István Altorjay
Dátum:2005
Megjegyzések:The role of nitric oxide (NO) in the pathophysiology of inflammatory bowel disease (IBD) is controversial. The aim of this study was to investigate the expression and localization of nitric oxide synthase isoforms (iNOS, eNOS) in IBD colonic mucosa. MATERIAL AND METHODS: Forty-four patients with IBD (24 ulcerative colitis (UC), 20 Crohn's disease (CD) and 16 controls) were investigated by colonoscopy. iNOS and eNOS in tissue sections was demonstrated by histochemistry (NADPH-diaphorase reaction) and immunohistochemistry. Cell type analysis and quantitative assessment of the iNOS immunoreactive (IR) cells and densitometry of iNOS in immunoblots were also performed. RESULTS: iNOS-IR cells were significantly numerous in inflamed mucosa of UC (64+/-4 cells/mm2) than in CD (4+/-2 cells/mm2). iNOS-IR/CD15-IR cells showed significant elevation in inflamed (i) versus uninflamed (u) UC mucosa (UCu 8+/-3%, UCi 85+/-10%) In CD, the percentage of iNOS-IR/CD68-IR cells was lower in inflamed sites (CDu 23+/-8%, CDi 4+/-3%). Immunoblot of biopsies revealed significant elevation of iNOS in active UC compared with uninflamed sites, whereas in CD no significant changes were detected. Differences were observed in eNOS and endothelial marker CD31 immunoreactivity. In patients with UC and in controls the ratios of eNOS/CD31-IR vessels were 82.3% and 92.0% respectively, whereas in CD the ratio was 8.3% with a concomitantly significant increase of CD31-IR vessels. The distribution and morphological characteristics of the NOS-IR inflammatory cells and endothelia were similar to those showing NADPH-diaphorase reactivity. CONCLUSIONS: Differences observed in the expression and distribution of NOS isoforms in immune and endothelial cells may contribute to better understanding of the structural and physiological changes in UC and CD.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
Crohn betegség
histopathológia
gyulladásos bélbetegség
nitrogénmonoxid szintáz
fekélyes bélgyulladás
Megjelenés:Scandinavian Journal of Gastroenterology 40 : 6 (2005), p. 670-680. -
További szerzők:Serfőző Zoltán Veréb Zoltán (1980-) (immunológus, mikrobiológus, molekuláris biológus) Hargitay Zoltán Lontay Beáta (1975-) (biokémikus) Erdődi Ferenc (1953-) (biokémikus) Bánfalvi Gáspár (1943-) (sejtbiológus, gyógyszerész) Nemes Zoltán (1942-) (patológus) Udvardy Miklós (1947-) (belgyógyász, haematológus) Altorjay István (1954-) (belgyógyász, gasztroenterológus, onkológus)
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2.

001-es BibID:BIBFORM001297
035-os BibID:(Wos)000222590300004 (Scopus)2442668507
Első szerző:Röszer Tamás (orvos, biológus)
Cím:Structural diversity of NADPH diaphorase-reactive enteral networks in Stylommatophora (Gastropoda, Pulmonata) / Tamás Röszer, Zsolt Jenei, Zoltán Serfözö, Zsolt Czimmerer, Gáspár Bánfalvi
Dátum:2004
Megjegyzések:In this work we investigated the involvement of putative nitric oxide (NO)-forming neurons in enteric plexuses of stylommatophoran gastropods. The nitric oxide synthase (NOS)-containing cells were detected by NADPH diaphorase (NADPHd) histochemistry in the entreral nervous systems of several stylommatophoran species (Achatinacea: Achatina fulica, Helicacea: Cepaea hortensis, Cepaea nemoralis, Discus rotundatus, Helicella obvia, Helix lucorum, Helix lutescens, Monachoides umbrosa, Trichia hispida, Zebrina detrita, Succineacea: Succinea putris, Vertiliginacea: Clausilia dubia, Zonitacea: Arion ater, Arion subfuscus, Limax maximus). We detected the NO synthesis of isolated midintestinal segments by Griess's quantification of nitrite, one end product of NO. Effects of the NOS substrate L-arginine and the NOS inhibitor N?-nitro-L-arginine (NOARG) were also tested on nitrite production. We found NADPHd-reactive neurons and extrinsic nerves with NADPHd-stained fibers within the myenteric and submucosal networks of the midintestine of investigated members of Helicacea, Succineacea, and Vertiliginacea families. These networks innervated the midintestinal musculature and several nerve cells of the myenteric and submucosal plexi. In investigated members of Achatinacea and Zonitacea, NADPHd-stained networks were not detectable within the digestive tract. Administration of 1 mM L-arginine elevated, whereas 2 mM of NOARG diminished, the nitrite levels of the NADPHd-stained networks containing midintestine in C. nemoralis and H. lucorum. Enteral NADPHd staining was not detected in A. ater and L. maximus, and the nitrite production was not affected by L-arginine. Our results indicate a possible, but evolutionarily not conserved, NO-mediated enteral transmission in stylommatophoran gastropods.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
NADPH diaforáz
nitrogénmonoxid szintáz
enterális idegrendszer
lábasfejűek
Megjelenés:Invertebrate Biology. - 123 : 2 (2004), p. 128-135. -
További szerzők:Jenei Zsolt Serfőző Zoltán Czimmerer Zsolt (1981-) (molekuláris biológus) Bánfalvi Gáspár (1943-) (sejtbiológus, gyógyszerész)
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3.

001-es BibID:BIBFORM001290
035-os BibID:(Wos)000187210800003 (Scopus)0348010590
Első szerző:Röszer Tamás (orvos, biológus)
Cím:A possible stimulatory effect of FMRFamide on neural nitric oxide production in the central nervous system of Helix lucorum L. / Tamás Röszer, Zsolt Jenei, Tamás Gáll, Olivér Nagy, Zsolt Czimmerer, Zoltán Serfözö, Károly Elekes, Gáspár Bánfalvi
Dátum:2004
Megjegyzések:The anatomical and functional relationship between neurons expressing nitric oxide (NO) synthase and molluscan cardioexcitatory (FMRFamide)-like neuropeptides was studied in the central ganglia of Helix lucorum (Pulmonata, Gastropoda), applying NADPHdiaphorase (NADPHd) histochemistry to visualize NO synthase and immunocytochemistry to demonstrate FMRFamide (FMRFa) at the light microscopic level. The NO production of the ganglia was detected by the colorimetric Griess determination of nitrite, a breakdown product of NO. Effects of the NO synthase substrate amino acid L-arginine, the NO synthase inhibitor Nomega-nitro-L-arginine (NOARG), synthetic FMRFa and the FMRFa sensitive ion channel blocker amiloride hydrochloride on nitrite production were also tested. NADPHd reaction labeled nerve cells and fibers in the procerebra, mesocerebra and metacerebra within the cerebral ganglia, and cell clusters in the postcerebral ganglia. FMRFa immunolabeling could be observed within subpopulations of NADPHd positive cells and in pericellular varicose fibers surrounding NADPHd stained neurons. Nitrite production of the ganglia was stimulated by L-arginine (10- 20 mM) but was decreased by NOARG (1-2 mM). Synthetic FMRFa (0.830-3.340 mM) increased the nitrite production in a dose dependent manner, but was ineffective in the presence of NOARG. Amiloride hydrochloride (7.890 mM) reduced the FMRFa evoked nitrite production in all ganglia. This is the first description of an anatomical relationship between putative NO producing and FMRFa containing cells, suggesting a possible regulatory role of FMRFa in the NO mediated signaling in an invertebrate nervous system. Copyright 2004 S. Karger AG, Basel
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
FMRFamid
nitrogén monoxid
neurokémia
gerinctelenek
fejlábúak
éticsiga
Megjelenés:Brain, Behavior and Evolution. - 63 : 1 (2004), p.23-33. -
További szerzők:Jenei Zsolt Gáll Tamás Nagy Olivér Czimmerer Zsolt (1981-) (molekuláris biológus) Serfőző Zoltán Elekes Károly Bánfalvi Gáspár (1943-) (sejtbiológus, gyógyszerész)
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4.

001-es BibID:BIBFORM001309
Első szerző:Szepessy Edit
Cím:Multiple subphases of DNA repair and poly (ADP-ribose) synthesis in Chinese hamster ovary (CHO-K1) cells / Edit Szepessy, Gabor Nagy, Zsolt Jenei, Zoltan Serfozo, Ildiko Csuka, Jill James, Gaspar Banfalvi
Dátum:2003
Megjegyzések:The two types of DNA synthesis as well as poly(ADP-ribose) biosynthesis were measured simultaneously in synchronized intact populations of CHO cells throughout the duration of S phase. Naturally occurring DNA fragmentation was detected by random primed oligonucleotide synthesis (ROPS assay). Fractions of synchronous cell populations were obtained by counterflow centrifugal elutriation. By gradually increasing the resolution of centrifugal elutriation multiple non-overlapping repair and replication peaks were obtained. The elutriation profile of DNA repair peaks corresponded to the DNA fragmentation pattern measured by ROPS assay. The number and position of poly(ADP-ribose) peaks during S phase resembled those seen in the DNA replication profile. Our results indicate that PAR synthesis is coupled to DNA replication serving the purpose of genomic stability.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
DNS repair alfázisok
PAR szintézis alfázisok
DNS replikáció
permeábilis sejtek
Megjelenés:European journal of cell biology. - 82 : 4 (2003), p. 201-207. -
További szerzők:Szemán-Nagy Gábor (1975-) (biológia tanár-molekuláris biológus) Jenei Zsolt Serfőző Zoltán Csuka Ildikó James, Jill Bánfalvi Gáspár (1943-) (sejtbiológus, gyógyszerész)
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