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001-es BibID:BIBFORM005978
Első szerző:Hadházy Csaba (anatómus)
Cím:Modification of the cell cycle of limb bud mesenchyme during in vitro cartilage differentiation / Hadházy, C., Szöllősi, J.
Dátum:1983
Megjegyzések:A consistent chondrogenesis takes place in micro high-density cultures derived from limb mesenchymal cells of chick embryos of stages 23-24. Flow-cytometric measurements of DNA content showed that cells in the phase of G1 or G0 made up 51% of the dispersed cell suspensions. The proportion of these cells increased to 71% by the onset of cartilage differentiation in day-2 cultures. This ratio was 84% when the voluminous matrix formation began on the 4th day of culturing. Thereafter, it increased to 90% by the 6th day, and to 93% by the 14th day. The results suggest that cartilage differentiates from G0 mesenchymal cells of the limb. In our measurements, however, the G0 phase includes all non-proliferative cell population which have identical DNA content with G1 cells. Therefore, the G0 phase contains also an increasing number of chondroblasts and chondrocytes as the chondrogenesis proceeds.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény hazai lapban
folyóiratcikk
Animal
Cartilage
Cell Cycle
Cell Differentiation
Cells,Cultured
Chick Embryo
cytology
Dna
Embryo
embryology
Extremities
Hungary
In Vitro
Interphase
Mesoderm
metabolism
Support,Non-U.S.Gov't
Time Factors
Megjelenés:Acta Biologica Hungarica. - 34 : 4 (1983), p. 407-414. -
További szerzők:Szöllősi János (1953-) (biofizikus)
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