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001-es BibID:BIBFORM006033
Első szerző:Feuerstein, Burt G.
Cím:alpha-Difluoromethylornithine alters calcium signaling in platelet-derived growth factor-stimulated A172 brain tumor cells in culture / Feuerstein, B. G., Szollosi, J., Basu, H. S., Marton, L. J.
Dátum:1992
Megjegyzések:alpha-Difluoromethylornithine (DFMO), an irreversible inhibitor of the polyamine biosynthetic enzyme ornithine decarboxylase, inhibits the growth of brain tumor cell lines and is undergoing clinical trials as a treatment for brain tumors. Platelet-derived growth factor (PDGF) is thought to regulate the growth and development of precursors of both normal and neoplastic astrocytic cells; calcium signaling is thought to play a role in the transduction of PDGF signals. Using laser fluorescence image cytometry, flow cytometry, and spectrofluorometry, we studied the effect of DFMO on the calcium signals induced by PDGF in A172 human glioblastoma cells. Four days of treatment with 5 mM DFMO substantially shortened PDGF-induced calcium signals. The effect was reversed more than 10 h but less than 24 h after putrescine treatment, even though polyamines were repleted 4 h after putrescine and spermidine were added. DFMO did not substantially affect intracellular calcium release or the timing of the opening and closing of plasma membrane calcium channels. These findings support the notion that calcium signaling may be a target for inhibitors of polyamine metabolism.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
analysis
Biogenic Polyamines
Brain Neoplasms
Calcium
Calcium Channels
Calcium Signaling
Cell Line
drug effects
Eflornithine
Flow Cytometry
Fluorescence
Glioblastoma
Human
Image Cytometry
Ion Channel Gating
metabolism
pathology
pharmacology
Platelet-Derived Growth Factor
Polyamines
Signal Transduction
Support, Non-U.S.Gov't
Support, U.S.Gov't,P.H.S.
Tumor Cells, Cultured
Megjelenés:Cancer Research. - 52 : 24 (1992), p. 6782-6789. -
További szerzők:Szöllősi János (1953-) (biofizikus) Basu, Hirak S. Marton, Laurence J.
Internet cím:elektronikus változat
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2.

001-es BibID:BIBFORM004934
Első szerző:Szöllősi János (biofizikus)
Cím:ERBB-2 (HER2/neu) gene copy number, p185HER-2 overexpression, and intratumor heterogeneity in human breast cancer / Szollosi, J., Balazs, M., Feuerstein, B. G., Benz, C. C., Waldman, F. M.
Dátum:1995
Megjegyzések:Amplification of the ERBB-2 (HER-2/neu) gene is accompanied by overexpression of its cell surface receptor product, p185HER-2. Heterogeneity has been observed for both the gene copy number and the level of overexpression of its protein product. To better understand their relationship, correlation between the level of cellular expression of p185HER-2 and ERBB-2 gene amplification was studied in four human breast cancer cell lines (BT-474, SK-BR-3, MDA-453, and MCF-7) and in a primary human breast tumor sample. The relative expression of p185HER-2 was measured by immunofluorescence by using flow and/or image cytometry while correlated DNA analysis was performed on the same cells by fluorescence in situ hybridization to determine ERBB-2 gene and chromosome 17 copy numbers. Marked heterogeneity was observed in both protein expression and ERBB-2 copy number. Despite this heterogeneity, and in accordance with previous studies, the average levels of p185HER-2 expression correlated well with average ERBB-2 gene copy numbers in the four lines examined (r = 0.99). When the relationship between copy number and protein expression was studied on a cell-by-cell basis, p185HER-2 expression correlated with both the absolute number of ERBB-2 gene copies/cell (r = 0.59-0.63) and chromosome 17 copy number (r = 0.45-0.61). It is of interest that there was weak or no correlation between p185HER-2 protein expression and the ERBB-2 copy number:chromosome 17 copy number ratio (r = 0.0-0.25). In more than one-half of cells expressing a high level of p185HER-2, the chromosome 17 copy number was high (two or three times the average copy number), whereas < 2% of an unselected population had a high chromosome 17 copy number. Bromodeoxyuridine incorporation indicated that the S-phase-labeling index was homogeneous across various p185HER-2-expressing subpopulations in the SK-BR-3 cell line. Analysis of the primary breast tumor sample showed results similar to the cell lines, supporting the strong possibility of a mechanistic link among p185HER-2 overexpression, ERBB-2 amplification, and high chromosome 17 copy number.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
analysis
biosynthesis
Breast Neoplasms
Cell Line
Chromosomes,Human,Pair 17
Dna
Female
Fluorescence
Gene Amplification
Gene Dosage
Genes,erbB-2
genetics
Human
Hybridization
Image Cytometry
Receptor,erbB-2
Support,Non-U.S.Gov't
Support,U.S.Gov't,P.H.S.
Tumor Cells,Cultured
Megjelenés:Cancer Research. - 55 : 22 (1995), p. 5400-5407. -
További szerzők:Balázs Margit (1952-) (sejtbiológus, molekuláris genetikus) Feuerstein, Burt G. Benz, Christopher C. Waldman, Frederick
Internet cím:elektronikus változat
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