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1.

001-es BibID:BIBFORM010350
035-os BibID:(scopus)67349251235 (wos)000267233000008
Első szerző:Friedländer Elza (biofizikus)
Cím:Corrigendum to ?ErbB-directed immunotherapy: Antibodies in current practice and promising new agents" [Immunol. Lett. 116/2 (2008) 126-140] / Friedlander, E., Barok, M., Szollosi, J., Vereb, G.
Dátum:2009
ISSN:0165-2478
Tárgyszavak:Orvostudományok Elméleti orvostudományok hozzászólás
folyóiratcikk
Antibodies
Megjelenés:Immunology Letters. - 124 : 1 (2009), p. 55-56. -
További szerzők:Barok Márk (1976-) (biofizikus) Szöllősi János (1953-) (biofizikus) Vereb György (1965-) (absztraktok, könyvfejezetek)
Internet cím:DOI
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2.

001-es BibID:BIBFORM004864
035-os BibID:(scopus)26444452209 (wos)000232299300016
Első szerző:Friedländer Elza (biofizikus)
Cím:Signal transduction of erbB receptors in trastuzumab (Herceptin) sensitive and resistant cell lines : local stimulation using magnetic microspheres as assessed by quantitative digital microscopy / Friedlander, E., Arndt-Jovin, D. J., Nagy, P., Jovin, T. M., Szollosi, J., Vereb, G.
Dátum:2005
ISSN:1552-4922
Megjegyzések:ErbB2 (HER-2), a member of the epidermal growth factor (EGF) receptor family, is a class I transmembrane receptor tyrosine kinase. Although erbB2 has no known physiologic ligand, it can form complexes with other members of the family and undergo transactivation of its very potent kinase activity, thereby initiating downstream signaling and cell proliferation. ErbB2 is a frequent pathologic marker in ductal invasive breast carcinomas and is targeted by using a specific humanized monoclonal antibody, trastuzumab (Herceptin). The antibody is effective in only 20% to 50% of erbB2-positive tumors, and this resistance, as yet poorly understood, constitutes a major therapeutic challenge. METHODS: Magnetic microspheres coated with ligands or antibodies are widely used for separation of proteins and cells and allow localized, high intensity, and precisely timed stimulation of cells. We used EGF- and trastuzumab-covered paramagnetic microspheres, quantitative confocal laser scanning microscopy, and digital image processing to investigate the (trans)activation of and local signal propagation from erbB1 and erbB2 on trastuzumab sensitive and resistant carcinoma cell lines expressing these receptors at high levels. RESULTS: On A431 cells expressing high levels of endogenous erbB1 and transfected erbB2-mYFP (A4-erbB2-mYFP F4 cell line), EGF-coupled-microspheres activated erbB1 and transactivated erbB2-mYFP. In two other cell lines with comparable erbB2 expression but lower levels of erbB1, EGF microspheres transactivated erbB2 less efficiently. Trastuzumab in solution activated erbB2 on A4-erbB2-mYFP and the trastuzumab sensitive SKBR-3 cells, but only negligibly on the resistant JIMT-1 cells that showed a 10 times higher K(d) for the antibody. Nevertheless, pronounced erbB2 activation and tyrosine phosphorylation could be detected after stimulation with trastuzumab-coupled microspheres in all cell lines, although transactivation of erbB1 was negligible. Receptor phosphorylation was restricted to the immediate proximity of the microspheres, i.e., receptor clusters external to these locations remained inactive. CONCLUSION: ErbB1 ligand and erbB2 specific antibody attached to magnetic microspheres are efficient tools in assessing erbB activation, localized signal propagation, and erbB heterodimer formation. Trastuzumab coupled to microspheres is more efficient at accessing erbB2 and activating it than trastuzumab in solution
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Animals
Antibodies
Antibodies,Monoclonal
Biophysics
Carcinoma
Cell Line
Cell Line,Tumor
Cell Proliferation
Cells
chemistry
drug effects
Drug Resistance,Neoplasm
Epidermal Growth Factor
genetics
Humans
Hungary
Ligands
Magnetics
metabolism
methods
Microscopy
Microspheres
pharmacology
Phosphorylation
Proteins
Receptor,erbB-2
Research
Signal Transduction
Solubility
Support
Trans-Activation (Genetics)
Megjelenés:Cytometry. Part A. - 67 : 2 (2005), p. 161-171. -
További szerzők:Arndt-Jovin, Donna J. Nagy Péter (1971-) (biofizikus) Jovin, Thomas M. Szöllősi János (1953-) (biofizikus) Vereb György (1965-) (biofizikus, orvos)
Internet cím:elektronikus változat
DOI
Borító:

3.

001-es BibID:BIBFORM005181
035-os BibID:(scopus)40149107884 (wos)000255092200005
Első szerző:Friedländer Elza (biofizikus)
Cím:ErbB-directed immunotherapy : antibodies in current practice and promising new agents / Elza Friedländer, Márk Barok, János Szöllősi, György Vereb
Dátum:2008
Megjegyzések:The ErbB family is well known for its significance in oncogenesis. As bad prognostic markers, overexpressed or mutated ErbB1 and ErbB2 have an important role in the molecular diagnosis of various cancers, but as membrane proteins, expressed often selectively in tumor tissues, they can be targeted with kinase inhibitors or therapeutic antibodies. In addition to trastuzumab, the first humanized antibody that was approved for the therapy of solid tumors by the FDA, now many humanized antibodies are in late clinical trials, or already approved. Conjugation of antibodies with radioactive isotopes, cytotoxic agents or liposomes loaded with chemotherapeutic drugs led to improved therapeutic efficiency over their parent "unarmed" antibodies. Novel, engineered antibody derivates with better pharmacodynamic properties are also available and allow the targeting of ErbB1 or ErbB2 positive cancers in a wider patient population. In this review, we discuss the biological and clinical background of ErbB targeting, and describe the most successful antibodies against ErbB1 (cetuximab, panitumumab, matuzumab, nimotuzumab, ICR62, mAb 528, ch806 and MDX-447) and ErbB2 (trastuzumab, pertuzumab, MDX-H210, 2B1, C6.5xscFv(NM3E2), ertumaxomab and FRP-5 derivates) that are in clinical trials or already approved, along with the various relevant conjugation and engineering strategies. Recent data pertinent to the prevalent problem of clinical resistance to treatment with trastuzumab are also discussed.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
agonists
analysis
Animal
Antibodies
Antibody conjugation
Antibody targeted delivery
Apoptosis
Biophysics
Brain
Carcinoma
Cell Line
Cell Proliferation
Cell Survival
Cells
Clinical trial
Cysteine
diagnosis
Dimerization
Down-Regulation
Drug Resistance
EGFR
Endocytosis
Engineered antibody
Epidermal Growth Factor
Epitopes
ErbB1
ErbB2
Fibroblasts
Glioblastoma
Glioma
Human
Humanized antibody
Hungary
In Vitro
Insulin
Membrane Proteins
Mice
Microscopy
Microspheres
Mutation
Necrosis
Paclitaxel
Phenotype
Phosphorylation
Prevalence
Progesterone
Proteins
radiotherapy
Research
Signal Transduction
therapy
toxicity
Trastuzumab resistance
Tumor Necrosis Factor
Tumor therapy/cancer therapy
Tyrosine
Up-Regulation
Megjelenés:Immunology Letters. - 116 : 2 (2008), p. 126-140. -
További szerzők:Barok Márk (1976-) (biofizikus) Szöllősi János (1953-) (biofizikus) Vereb György (1965-) (biofizikus, orvos)
Internet cím:elektronikus változat
DOI
elektronikus változat
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4.

001-es BibID:BIBFORM111996
Első szerző:Nagy Péter (biofizikus)
Cím:Novel single cell fluorescence approaches in the investigation of signaling at the cellular level / Péter Nagy, György Vereb, Janine N. Post, Elza Friedländer, János Szőllősi
Dátum:2005
ISSN:0932-2353
ISBN:9783540250647
Tárgyszavak:Orvostudományok Elméleti orvostudományok könyvfejezet
könyvrészlet
Megjelenés:Biophysical aspects of transmembrane signaling / szerk. Damjanovich Sándor. - p. 33-70. -
További szerzők:Vereb György (1965-) (biofizikus, orvos) Post, Janine N. Friedländer Elza (1980-) (biofizikus) Szöllősi János (1953-) (biofizikus)
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
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5.

001-es BibID:BIBFORM050093
Első szerző:Petrás Miklós (orvos)
Cím:Molecular interactions of ErbB1 (EGFR) and integrin-β1 in astrocytoma frozen sections predict clinical outcome and correlate with Akt-mediated in vitro radioresistance / Miklós Petrás, Tamás Lajtos, Elza Friedländer, Álmos Klekner, Éva Pintye, Burt G. Feuerstein, János Szöllősi, György Vereb
Dátum:2013
ISSN:1522-8517
Megjegyzések:INTRODUCTION: Treatment of astrocytoma is frequently hampered by radioresistance of the tumor. In addition to overexpression of ErbB1/EGFR, functional crosstalk between receptor tyrosine kinases and cell adhesion molecules may also contribute to therapy resistance. METHODS: Acceptor photobleaching FRET was implemented on frozen sections of clinical astrocytoma to check the role of ErbB1-integrin-beta1 interaction. U251 glioma subclones were obtained by introducing extra CHR7 material or the ErbB1 gene to test the relevance and mechanism of this interaction in vitro. RESULTS: Grade IV tumors showed higher ErbB1 and integrin-beta1 expression and greater ErbB1-integrin-beta1 heteroassociation than did grade II tumors. Of these, the extent of molecular association was a single determinant of tumor grade and prognosis in stepwise logistic regression. In vitro, integrin-beta1 was upregulated, and radiosensitivity was diminished by ectopic ErbB1 expression. Great excess of ErbB1 provided colony forming advantage over medium excess but did not yield better radiation resistance or faster proliferation and decreased to medium level over time, whereas integrin-beta1 levels remained elevated and defined the extent of radioresistance. Increased expression of ErbB1 and integrin-beta1 was paralleled by decreasing ErbB1 homoassociation and increasing ErbB1-integrin-beta1 heteroassociation. Microscopic two-sided FRET revealed that pixels with higher ErbB1-integrin-beta1 heteroassociation exhibited lowed ErbB1 homoassociation, indicating competition for association partners among these molecules. Boosted Akt phosphorylation response to EGF accompanied this shift toward heteroassociation, and the consequentially increased radioresistance could be reverted by inhibiting PI3K. CONCLUSION: The clinically relevant ErbB1-integrin-beta1 heteroassociation may be used as a target of both predictive diagnostics and molecular therapy
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Biophysics
Cell Adhesion
Cell Adhesion Molecules
cell biology
EGFR
ErbB1
EXPRESSION FRET
Glioma
Hungary
In Vitro
methods
molecular interaction
Phosphorylation
Photobleaching
Receptor
tyrosine kinase
Research
therapy
TUMORS
Tyrosine
Molekulatudomány
Doktori iskola
Megjelenés:Neuro-Oncology. - 15 : 8 (2013), p. 1027-1040. -
További szerzők:Lajtos Tamás Friedländer Elza (1980-) (biofizikus) Klekner Álmos (1970-) (idegsebész) Pintye Éva (1955-) (fizikus) Feuerstein, Burt G. Szöllősi János (1953-) (biofizikus) Vereb György (1965-) (biofizikus, orvos)
Pályázati támogatás:K75752
OTKA
NK101337
OTKA
F-049050
OTKA
ETT 523/2003
Egyéb
ETT 362-01/2009
Egyéb
TÁMOP-4.2.1/B-09/1/KONV-2010-0007
TÁMOP
TÁMOP-4.2.2/B-10/1-2010-0024
TÁMOP
TÁMOP-4.2.2/A-11/1/KONV-2012-0025
TÁMOP
Internet cím:Szerző által megadott URL
DOI
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6.

001-es BibID:BIBFORM079931
035-os BibID:(cikkazonosító)3370 (scopus)85071327332 (wos)000477041100254
Első szerző:Vámosi György (biofizikus)
Cím:EGF Receptor Stalls upon Activation as Evidenced by Complementary Fluorescence Correlation Spectroscopy and Fluorescence Recovery after Photobleaching Measurements / Vámosi György, Friedländer-Brock Elza, Ibrahim Shehu M., Brock Roland, Szöllősi János, Vereb György
Dátum:2019
ISSN:1661-6596 1422-0067
Megjegyzések:To elucidate the molecular details of the activation-associated clustering of epidermal growth factor receptors (EGFRs), the time course of the mobility and aggregation states of eGFP tagged EGFR in the membranes of Chinese hamster ovary (CHO) cells was assessed by in situ mobility assays. Fluorescence correlation spectroscopy (FCS) was used to probe molecular movements of small ensembles of molecules over short distances and time scales, and to report on the state of aggregation. The diffusion of larger ensembles of molecules over longer distances (and time scales) was investigated by fluorescence recovery after photobleaching (FRAP). Autocorrelation functions could be best fitted by a two-component diffusion model corrected for triplet formation and blinking. The slow, 100?1000 ms component was attributed to membrane localized receptors moving with free Brownian diffusion, whereas the fast, ms component was assigned to cytosolic receptors or their fragments. Upon stimulation with 50 nM EGF, a significant decrease from 0.11 to 0.07 ?m2/s in the diffusion coefficient of membrane-localized receptors was observed, followed by recovery to the original value in ~20 min. In contrast, the apparent brightness of diffusing species remained the same. Stripe FRAP experiments yielded a decrease in long-range molecular mobility directly after stimulation, evidenced by an increase in the recovery time of the slow component from 13 to 21.9 s. Our observations are best explained by the transient attachment of ligand-bound EGFRs to immobile or slowly moving structures such as the cytoskeleton or large, previously photobleached receptor aggregates.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
Fluorescence correlation spectroscopy
FCS
fluorescence recovery after photobleaching
FRAP
epidermal growth factor receptor
translational diffusion
EGFR?eGFP fusion protein
Megjelenés:International Journal Of Molecular Sciences. - 20 : 13 (2019), p. 1-22. -
További szerzők:Friedländer Elza (1980-) (biofizikus) Ibrahim, Shehu M. Brock Roland Szöllősi János (1953-) (biofizikus) Vereb György (1965-) (biofizikus, orvos)
Pályázati támogatás:K119690
OTKA
NN129371
OTKA
GINOP-2.3.2-15-2016-00050
GINOP
GINOP-2.3.3-15-2016-00003
GINOP
Internet cím:Szerző által megadott URL
DOI
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7.

001-es BibID:BIBFORM002771
Első szerző:Vereb György (biofizikus, orvos)
Cím:Fluorescence correlation spectroscopy of living cells / Vereb G., Ujlaky-Nagy L., Friedländer E., Vámosi G., Szöllősi J.
Dátum:2007
Tárgyszavak:Természettudományok Biológiai tudományok könyvfejezet
könyvrészlet
Cells
Fluorescence
Microscopy
Research
Megjelenés:Modern research and educational topics in microscopy / ed. Mendez-Vilas, A., Labajos-Broncano, L. - p. 848-854.
További szerzők:Ujlaky-Nagy László (1977-) (biofizikus) Friedländer Elza (1980-) (biofizikus) Vámosi György (1967-) (biofizikus) Szöllősi János (1953-) (biofizikus)
Internet cím:elektronikus változat
elektronikus változat
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