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1.

001-es BibID:BIBFORM050042
035-os BibID:PMID:23504771
Első szerző:Fábián Ákos István (aneszteziológus)
Cím:TripleFRET measurements in flow cytometry / Ákos Fábián, Gábor Horváth, György Vámosi, György Vereb, János Szöllősi
Dátum:2013
Megjegyzések:A frequently used method for viewing protein interactions and conformation, Forster (fluorescence) resonance energy transfer (FRET), has traditionally been restricted to two fluorophores. Lately, several methods have been introduced to expand FRET methods to three species. We present a method that allows the determination of FRET efficiency in three-dye systems on a flow cytometer. TripleFRET accurately reproduces energy transfer efficiency values measured in two-dye systems, and it can indicate the presence of trimeric complexes, which is not possible with conventional FRET methods. We also discuss the interpretation of energy transfer values obtained with tripleFRET in relation to spatial distribution of labeled molecules, specifically addressing the limitations of using total energy transfer to determine molecular distance
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
analysis
Antibodies
Antibodies,Monoclonal,Humanized
article
Biophysics
cell biology
Cell Line
chemistry
cytometry
Dyes
Energy Transfer
ENERGY-TRANSFER
FLOW
Flow Cytometry
Fluorescence
Fluorescence Resonance Energy Transfer
fluorescent dye
Fluorescent Dyes
FRET
Humans
Hungary
Immunoconjugates
immunology
methods
Protein Binding
PROTEIN INTERACTIONS
Research
Research Support
resonance energy transfer
Staining and Labeling
statistics & numerical data
Support
Megjelenés:Cytometry Part A. - 83 : 4 (2013), p. 375-385. -
További szerzők:Horváth Gábor (1974-) (biofizikus) Vámosi György (1967-) (biofizikus) Vereb György (1965-) (biofizikus, orvos) Szöllősi János (1953-) (biofizikus)
Pályázati támogatás:NK 101337
OTKA
K75752
OTKA
TÁMOP-4.2.2-08/1-2008-0019
TÁMOP
TÁMOP-4.2.1/B-09/1/KONV-2010-0007
TÁMOP
Receptor tirozin kinázok mint terápiás célpontok : működésük szabályozásának, és a közöttük fellépő molekuláris kölcsönhatások vizsgálata
TÁMOP-4.2.2.A-11/1/KONV-2012-0025
TÁMOP
Baross Gábor Program REG_EA_09-1-2009-0010
Egyéb
K77600
OTKA
K103965
OTKA
Internet cím:DOI
Intézményi repozitóriumban (DEA) tárolt változat
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2.

001-es BibID:BIBFORM043019
035-os BibID:PMID:22997049
Első szerző:Fábián Ákos István (aneszteziológus)
Cím:The hitchhikers guide to cancer stem cell theory : markers, pathways and therapy / Ákos Fábián, György Vereb, János Szöllősi
Dátum:2013
Megjegyzések:Cancer stem cell (CSC) biology is a rapidly developing field within cancer research. CSCs are postulated to be a unique cell population exclusively capable of infinite self renewal, multilineage differentiation and with ability to evade conventional cytotoxic cancer therapy. These traits distinguish CSCs from their more differentiated counterparts, which possess only limited or no potential for self renewal and tumor initiation. Therefore, CSCs would be the driving motor of malignant growth and therapy resistance. Accordingly, successful cancer treatment would need to eliminate this highly potent group of cells, since even small residual numbers would suffice to recapitulate the disease after therapy. Putative CSCs has been identified in a broad range of human malignancies and several cell surface markers have been associated with their stem cell phenotype. Despite all efforts, a pure CSC population has not been isolated and often in vitro clonogenic and in vivo tumorigenic potential is found in several cell populations with occasionally contradictory surface marker signatures. Here, we give a brief overview of recent advances in CSC theory, including the signaling pathways in CSCs that also appear crucial for stem cells homeostasis in normal tissues. We discuss evidence for the interaction of CSCs with the stromal tumor environment. Finally, we review the emerging potentially effective CSC-targeted treatment strategies and their future role in therapy. (c) 2012 International Society for Advancement of Cytometry
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
article
Biophysics
cell biology
cell surface
Cells
cytometry
differentiation
GROWTH
Homeostasis
Human
Hungary
In Vitro
Phenotype
Research
review
stem cell
therapy
Megjelenés:Cytometry. Part A. - 83 : 1 (2013), p. 62-71. -
További szerzők:Vereb György (1965-) (biofizikus, orvos) Szöllősi János (1953-) (biofizikus)
Pályázati támogatás:NK 101337
OTKA
K 75752
OTKA
TÁMOP-4.2.1/B-09/1/KONV-2010-0007
TÁMOP
Receptor tirozin kinázok mint terápiás célpontok: működésük szabályozásának, és a közöttük fellépő molekuláris kölcsönhatások vizsgálata
Baross Gábor Program REG_EA_09-1-2009-0010
Egyéb
Internet cím:DOI
Intézményi repozitóriumban (DEA) tárolt változat
Borító:

3.

001-es BibID:BIBFORM015619
Első szerző:Fábián Ákos István (aneszteziológus)
Cím:Strength in numbers : effects of Acceptor Abundance on FRET Efficiency / Ákos I. Fábián, Tünde Rente, János Szöllősi, László Mátyus, Attila Jenei
Dátum:2010
Megjegyzések:Fluorescence resonance energy transfer (FRET) is a strongly distance-dependent process between a donor and an acceptor molecule, which can be used for sensitive distance measurements and characterization of molecular interactions at the nanometer level. The original mathematical description of this process, however, is only valid for the interaction of one donor with one acceptor. This criterion is not always met, especially in biological systems, where multiple structures can interact simultaneously, often making distance estimations based on transfer efficiency values error-prone. Herein we investigate how the interaction of multiple acceptors and donors influences the transfer efficiency value in an intramolecular cellular FRET system by manipulating the fluorophore/protein ratio of the fluorophore-conjugated antibodies. We show that the labeling ratio of the acceptor has the largest influence on measured transfer efficiency and decreasing or increasing the acceptor labeling ratio can be utilized to manipulate the FRET response of the acceptor-donor pair and therefore is a tool for optimizing sensitivity of FRET measurements
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Antibodies
article
BINDING-SITE
BY-CELL BASIS
cell surface receptors
chemistry
Energy Transfer
Molekuláris Medicina
FLOW
Flow Cytometry
Fluorescence
FLUORESCENCE RESONANCE
Fluorescence Resonance Energy Transfer
FRET
HIGH-THROUGHPUT FRET
Hungary
IMAGING MICROSCOPY
LIVING CELLS
molecular interaction
PROTEIN INTERACTIONS
resonance energy transfer
RESONANCE ENERGY-TRANSFER
SPECTROSCOPIC RULER
OTKA::1
MAB::3.1
egyetemen (Magyarországon) készült közlemény
Megjelenés:Chemphyschem. - 11 : 17 (2010), p. 3713-3721. -
További szerzők:Rente Tünde Szöllősi János (1953-) (biofizikus) Mátyus László (1956-) (biofizikus) Jenei Attila (1966-) (biofizikus)
Pályázati támogatás:TÁMOP-4.2.1/B-09/1/KONV-2010-0007
TÁMOP
Membrán dinamika
K 77600
OTKA
K 68763
OTKA
TÁMOP-4.2.2-08/1-2008-0019
TÁMOP
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
DOI
Borító:

4.

001-es BibID:BIBFORM010349
035-os BibID:(scopus)59049105873 (wos)000262061700007
Első szerző:Fábián Ákos István (aneszteziológus)
Cím:Die Hard : are cancer stem cells the Bruce Willises of tumor biology? / Fabian, A., Barok, M., Vereb, G., Szollosi, J.
Dátum:2009
ISSN:1552-4922 (Print)
Megjegyzések:In recent years, an exponentially growing number of studies have focused on identifying cancer stem cells (CSC) in human malignancies. The rare CSCs could be crucial in controlling and curing cancer: through asymmetric division CSCs supposedly drive tumor growth and evade therapy with the help of traits shared with normal stem cells such as quiescence, self-renewal ability, and multidrug resistance pump activity. Here, we give a brief overview of techniques used to confirm the stem cell-like behavior of putative CSCs and discuss markers and methods for identifying, isolating, and culturing them. We touch on the limitations of each marker and why the combined use of CSC markers, in vitro and in vivo assays may still fail to identify all relevant CSC populations. Finally, the various experimental findings supporting and contradicting the CSC hypothesis are summarized. The large number of tumor types thus far with a subpopulation of uniquely tumorigenic and therapy resistant cells suggests that despite the unanswered questions and inconsistencies, the CSC hypothesis has a legitimate role to play in tumor biology. At the same time, experimental evidence supporting the established alternative theory of clonal evolution can be found as wen. Therefore, a model that describes cancer initiation and progression should combine elements of clonal evolution and CSC theory.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Cells
Human
In Vitro
methods
therapy
Megjelenés:Cytometry. Part A. - 75A : 1 (2009), p. 67-74. -
További szerzők:Barok Márk (1976-) (biofizikus) Vereb György (1965-) (biofizikus, orvos) Szöllősi János (1953-) (biofizikus)
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
DOI
Borító:

5.

001-es BibID:BIBFORM042481
035-os BibID:(scopus)40049093067 (wos)000253576200006
Első szerző:Fazekas Zsolt (biofizikus)
Cím:Two-sided fluorescence resonance energy transfer for assessing molecular interactions of up to three distinct species in confocal microscopy / Zsolt Fazekas, Miklós Petrás, Ákos Fábián, Zsuzsanna Pályi-Krekk, Péter Nagy, Sándor Damjanovich, György Vereb, János Szöllősi
Dátum:2008
ISSN:1552-4922 1552-4930
Megjegyzések:The role of the expression patterns of proteins involved in oncogenesis can be understood after characterizing their multimolecular interactions. Conventional FRET methods permit the analysis of interaction between two molecular species at the most, which necessitates the introduction of new approaches for studying multicomponent signaling complexes. Flow cytometric as well as microscopic donor (dbFRET) and acceptor (abFRET) photobleaching FRET measurements were performed to determine the association states of ErbB2, b1-integrin, and CD44 receptors. Based on consecutively applied abFRET and dbFRET methods (two-sided FRET), the relationship of b1-integrin?ErbB2 heteroassociation to ErbB2 homoassociation and of b1-integrin?ErbB2 heteroassociation to ErbB2?CD44 heteroassociation was studied by correlating pixel-by-pixel FRET values of the corresponding abFRET and dbFRET images in contour plots. Anticorrelation was observed between b1-integrin?ErbB2 heteroassociation and ErbB2 homoassociation on trastuzumab sensitive N87 and SK-BR-3 cells, while modest positive correlation was found between b1-integrin?ErbB2 and ErbB2?CD44 heteroassociationon trastuzumab resistant MKN-7 cells. The FRET efficiency values of b1-integrin?ErbB2 heteroassociation were markedly higher at the focal adhesion regions on attachedcells than those measured by flow cytometry on detached cells. In conclusion, we implemented an experimental set-up termed two-sided FRET for correlating two pairwiseinteractions of three arbitrarily chosen molecular species. On the basis of our results, we assume that the homoassociation state of ErbB2 is dynamically modulated by its interaction with b1-integrins.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
beta1-integrin
abFRET
analysis
Biophysics
Carcinoma
CD44
Cell Adhesion
Cell Adhesion Molecules
Cell Line
Cells
Cholera Toxin
Confocal
Confocal microscopy
cytology
dbFRET
Energy Transfer
ErbB2
Flow Cytometry
Fluorescein
Fluorescence
fluorescence microscopy
Fluorescence Resonance Energy Transfer
FRET
Hiv-1
Human
Hungary
Image Cytometry
Image Processing
Kinetics
Laser scanning confocal microscopy
Luminescence
methods
Microscopy
Photobleaching
Protein-protein interactions
Proteins
Receptor tyrosine kinase
Research
Signal Transduction
Software
therapy
Trastuzumab resistance
tsFRET
Tyrosine
egyetemen (Magyarországon) készült közlemény
Megjelenés:Cytometry Part A. - 73 : 3 (2008), p. 209-219. -
További szerzők:Petrás Miklós (1977-) (orvos) Fábián Ákos István (1982-) (aneszteziológus) Pályiné Krekk Zsuzsanna (1974-) (molekuláris biológus) Nagy Péter (1971-) (biofizikus) Damjanovich Sándor (1936-2017) (biofizikus) Vereb György (1965-) (biofizikus, orvos) Szöllősi János (1953-) (biofizikus)
Internet cím:Szerző által megadott URL
DOI
Intézményi repozitóriumban (DEA) tárolt változat
Borító:

6.

001-es BibID:BIBFORM042654
035-os BibID:(scopus)19444380683 (wos)000229353200007
Első szerző:Horváth Gábor (biofizikus)
Cím:Selecting the right fluorophores and flow cytometer for fluorescence resonance energy transfer measurements / Gábor Horváth, Miklós Petrás, Gergely Szentesi, Ákos Fábián, John W. Park, György Vereb, János Szöllősi
Dátum:2005
ISSN:1552-4922 1552-4930
Megjegyzések:BACKGROUND:Fluorescence resonance energy transfer applied in flow cytometry (FCET) is an excellent tool for determining supramolecular organization of biomolecules at the cell surface or inside the cell. Availability of new fluorophores and cytometers requires the establishment of fluorophore dye pairs most suitable for FCET measurements.METHODS:A gastric tumor cell line (N87) was labeled for major histocompatibility complex class I heavy chain and beta2-microglobulin with antibodies conjugated with fluorescein- and indocarbocyanine-like fluorophores and analyzed in FCET measurements on a cell-by-cell basis using three flow cytometers: FACSCalibur, FACSDiVa, and FACSArray.RESULTS:Normalized fluorescence intensity values were measured and normalized energy transfer efficiencies, spectral overlap integrals, and crucial dye- and instrument-dependent parameters were calculated for all matching pairs of seven fluorophores on the three commercial cytometers. The most crucial parameter in determining the applicability of the donor-acceptor pairs was the normalized fluorescence intensity and the least important one was the spectral overlap.CONCLUSIONS:On the basis of available laser lines, the optimal dye pair for all three cytometers is the Alexa546-Alexa647 pair, which produces high energy transfer efficiency values and has the best spectral characteristics with regard to laser excitation, detection of emission, and spectral overlap.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
fluorescence resonance energy transfer
flow cytometry
donor-acceptor pair
egyetemen (magyarországon) készült közlemény
Megjelenés:Cytometry. Part A. - 65A : 2 (2005), p. 148-157. -
További szerzők:Petrás Miklós (1977-) (orvos) Szentesi Gergely (1976-) (kémia-fizika tanár) Fábián Ákos István (1982-) (aneszteziológus) Park, John W. Vereb György (1965-) (biofizikus, orvos) Szöllősi János (1953-) (biofizikus)
Internet cím:Szerző által megadott URL
DOI
Intézményi repozitóriumban (DEA) tárolt változat
Borító:
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