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1.

001-es BibID:BIBFORM006025
Első szerző:Balázs Margit (sejtbiológus, molekuláris genetikus)
Cím:Fluorescent tetradecanoylphorbol acetate : a novel probe of phorbol ester binding domains / Balázs, M., Szöllösi, J., Lee, W. C., Haugland, R. P., Guzikowski, A. P., Fulwyler, M. J., Damjanovich, S., Feuerstein, B. G., Pershadsingh, H. A.
Dátum:1991
Megjegyzések:Protein kinase C (PKC) has a prominent role in signal transduction of many bioactive substances. We synthesized the fluorescent derivative, phorbol-13-acetate-12-N-methyl-N-4-(N,N'-di(2-hydroxyethyl)amino)-7-n itr obenz-2-oxa-1,3-diazole-aminododecanoate (N-C12-Ac(13)) of 12-O-tetradecanoylphorbol-13-acetate (TPA) to monitor the location of phorbol ester binding sites and evaluate its potential use as a probe of PKC in viable cells. The excitation maximum wavelength of N-C12-Ac(13) is close to 488 nm, facilitating its use in argon-ion laser flow and imaging cytometry. When incubated with 100 nM N-C12-Ac(13) at 25 degrees C, P3HR-1 Burkitt lymphoma cells accumulated the dye rapidly, reaching maximum fluorescence within 25 min, 20-fold above autofluorescence. Addition of unlabeled TPA significantly decreased the fluorescence of N-C12-Ac(13) stained cells in a dose-dependent manner indicating specific displacement of the bound fluoroprobe. Competitive displacement of [3H]-phorbol-12,13-dibutyrate ([3H]-PBu2) from rat brain cytosol with N-C12-Ac(13) gave an apparent dissociation constant (Kd) of 11 nM. N-C12-Ac(13) possessed biological activity similar to TPA. Like TPA (final concentration 65 nM) N-C12-Ac(13), at a lower concentration (51 nM), induced expression of Epstein-Barr viral glycoprotein in P3HR-1 cells, differentiation of promyelocytic HL60 cells, and caused predicted changes in the mitotic cycle of histiocytic DD cells. Microspectrofluorometric images of single cells labeled with N-C12-Ac(13) showed bright fluorescence localized intracellularly and dim fluorescence in the nuclear region, consistent with dye binding mainly to cytoplasmic structures and/or organelles and being mostly excluded from the nucleus. Because of the high level of non-specific binding of N-C12-Ac(13), this probe is not ideal for visualizing PKC in intact cells, but would be a valuable fluoroprobe to investigate the kinetic properties of purified PKC. Also, knowledge gained from these studies allows us to predict structures of fluorescent phorbols likely to have less non-specific binding and, consequently, be potentially useful for monitoring PKC in viable cells.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
analysis
Animal
Antigens,Viral
Binding Sites
Brain Chemistry
Cell Cycle
chemical synthesis
Cytosol
Dyes
Female
Flow Cytometry
Fluorescence
Fluorescence Polarization
Fluorescent Dyes
Gene Expression
Herpesvirus 4,Human
Image Processing,Computer-Assisted
immunology
metabolism
Oxadiazoles
pharmacology
Phorbol Esters
Protein Kinase C
Rats
Rats,Inbred Strains
Signal Transduction
Support,Non-U.S.Gov't
Support,U.S.Gov't,P.H.S.
Tetradecanoylphorbol Acetate
Tumor Cells,Cultured
Megjelenés:Journal of Cellular Biochemistry. - 46 : 3 (1991), p. 266-276. -
További szerzők:Szöllősi János (1953-) (biofizikus) Lee, W. C. Haugland, R. P. Guzikowski, A. P. Fulwyler, Mack J. Damjanovich Sándor (1936-2017) (biofizikus) Feuerstein, Burt G. Pershadsingh, Harrihar A.
Internet cím:elektronikus változat
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2.

001-es BibID:BIBFORM005965
Első szerző:Balázs Margit (sejtbiológus, molekuláris genetikus)
Cím:Accessibility of cell surface thiols in human lymphocytes is altered by ionophores or OKT-3 antibody / Margit Balázs, János Matkó, János Szöllösi, László Mátyus, Mack J. Fulwyler, Sándor Damjanovich
Dátum:1986
Megjegyzések:The accessibility of cell surface sulfhydryl groups in human peripheral lymphocytes was investigated with 5,5'-dithiobis-(2-nitrobenzoic acid) in the presence and absence of ionophore antibiotics and the monoclonal antibody, OKT-3. Only a few accessible protein thiols have been found on the cells as demonstrated by labeling with a fluorescent non-penetrating thiol-marker, monobromotrimethyl-ammoniobimane and the subsequent gel electrophoretic analysis of the protein pattern. Difference spectrophotometric measurement of thiol-DTNB reaction revealed that ionophores altering the transmembrane potential induced an enhanced cell surface thiol-exposure on the minute time scale. The effect showed a dependence on the external concentration of the cations. The binding of monoclonal antibody, OKT-3, directed against T3 complexes, resulted in a similar, concentration-dependent increase of thiol-accessibility. These data are interpreted as early membrane-effects of ionophores and the specific antibody including changes in the conformational equilibrium or vertical displacements of certain membrane proteins. These events are likely to be coupled to the changes in the transmembrane potential of the lymphocytes.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Ammonium Compounds
analysis
Antibiotics
Antibodies,Monoclonal
blood
Cell Membrane
Dithionitrobenzoic Acid
drug effects
Electrophoresis,Polyacrylamide Gel
Human
Ionophores
Lymphocytes
Membrane Proteins
metabolism
pharmacology
Spectrometry,Fluorescence
Sulfhydryl Compounds
Support,Non-U.S.Gov't
Support,U.S.Gov't,P.H.S.
Megjelenés:Biochemical and Biophysical Research Communications. - 140 : 3 (1986), p. 999-1006. -
További szerzők:Matkó János (1952-) (biológus) Szöllősi János (1953-) (biofizikus) Mátyus László (1956-) (biofizikus) Fulwyler, Mack J. Damjanovich Sándor (1936-2017) (biofizikus)
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Intézményi repozitóriumban (DEA) tárolt változa
DOI
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3.

001-es BibID:BIBFORM005968
035-os BibID:(scopus)0023260317
Első szerző:Damjanovich Sándor (biofizikus)
Cím:Cyclosporin depolarizes human lymphocytes : earliest observed effect on cell metabolism / S. Damjanovich, A. Aszalos, S. A. Mulhern, J. Szollosi, M. Balazs, L. Tron, M. J. Fulwyler
Dátum:1987
Megjegyzések:Cyclosporin A (CsA) produced dose-dependent membrane depolarization of human peripheral blood lymphocytes. The phenomenon was investigated applying the membrane potential probe dihexyloxacarbocyanine iodide in a flow cytometer in combination with ionophores, hormones and monoclonal antibodies binding to different subclasses of lymphocytes and the anti-interleukin 2 receptor antibody. Human interferon-gamma abolished the depolarizing effect of cyclosporin on lymphocytes. Interleukin 2 caused depolarization and also enhanced the effect of CsA. OKT4 and OKT8 monoclonal antibodies slightly hindered depolarization by CsA while OKT3, OKT11 and OKIa1 antibodies had no such effect. Valinomycin decreased CsA's effect on the membrane potential while the ionophore A-23187 and ionomycin caused depolarizations that were additive with CsA's. CsA treatment released the isotope from 42K-loaded human lymphocytes in a dose-dependent fashion. CsA addition increased intracellular calcium content. CsA decreased the motional freedom of a spin probe in the membrane, but did not hinder the binding of fluoresceinated antibodies to the cell surface. These results suggest immediate alteration in membrane structure upon CsA treatment, causing potassium leakage and calcium ion uptake. These are the earliest detected effects of CsA on cells so far.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Antibodies,Monoclonal
blood
Calcium
Carbocyanines
Cell Membrane
classification
Cyclosporins
Cytoplasm
Dimethyl Sulfoxide
drug effects
Electron Spin Resonance Spectroscopy
Flow Cytometry
Human
immunology
Interferon Type II
Interleukin-2
Intracellular Membranes
Ion Channels
Ionomycin
Ionophores
Lymphocytes
Membrane Fluidity
Membrane Potentials
metabolism
methods
pharmacology
Potassium
Potassium Radioisotopes
Spectrometry,Fluorescence
ultrastructure
Valinomycin
Megjelenés:European Journal of Immunology. - 17 : 6 (1987), p. 763-768. -
További szerzők:Aszalos Adorján Mulhern, Sally Szöllősi János (1953-) (biofizikus) Balázs Margit (1952-) (sejtbiológus, molekuláris genetikus) Trón Lajos (1941-) (biofizikus) Fulwyler, Mack J.
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4.

001-es BibID:BIBFORM006015
Első szerző:Szöllősi János (biofizikus)
Cím:Flow cytometric measurements of fluorescence energy transfer using single laser excitation / Szöllösi, J., Mátyus, L., Trón, L., Balázs, M., Ember, I., Fulwyler, M. J., Damjanovich, S.
Dátum:1987
Megjegyzések:Flow cytometric energy transfer (FCET) measurements between labeled specific sites of cell surface elements (Szollosi et al., Cytometry, 5:210-216, 1984) have been extended in a simplified form using a flow cytometer equipped with single excitation beam. This versatile and easily applicable method has several advantages over any nonflow cytometric (i.e., spectrofluorimetric) energy transfer measurements on cell surfaces: The labeled ligands can be applied in excess, without washing, thereby enabling the investigation of relatively labile receptor-ligand complexes. Contributions of signals from cell debris, from cell aggregates, or from nonviable cells can be avoided by gating the data collection on the light scatter signal. The heterogeneity of the cell population with respect to the proximity of the labeled binding sites can be studied. In the cases of homologous ligands or of ligands binding to the same molecule but at different epitopes, the determination of fluorescence resonance energy transfer efficiency values can be carried out on a cell-by-cell basis, offering data on intramolecular conformational changes. This modified FCET method enabled us to demonstrate the uniform density of glycoproteins, specific for Con A binding, in the plasma membrane of normal and Gross virus leukemic mouse cells of different sizes. The utility of this procedure has also been demonstrated by using the mean fluorescence intensities of the distribution curves in the calculation of the fluorescence energy transfer efficiency.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
analysis
Antigens,Surface
Binding Sites
diagnostic use
Energy Transfer
Epitopes
Flow Cytometry
Fluorescein
Fluoresceins
Fluorescence
Glycoproteins
instrumentation
Lasers
Ligands
Light
Lymphocytes
Lymphoma
Receptors,Concanavalin A
Rhodamines
Spectrometry,Fluorescence
Support,U.S.Gov't,Non-P.H.S.
Megjelenés:Cytometry. - 8 : 2 (1987), p. 120-128. -
További szerzők:Mátyus László (1956-) (biofizikus) Trón Lajos (1941-) (biofizikus) Balázs Margit (1952-) (sejtbiológus, molekuláris genetikus) Ember István Fulwyler, Mack J. Damjanovich Sándor (1936-2017) (biofizikus)
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Intézményi repozitóriumban (DEA) tárolt változa
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5.

001-es BibID:BIBFORM006011
Első szerző:Szöllősi János (biofizikus)
Cím:Flow cytometric resonance energy transfer measurements support the association of a 95-kDa peptide termed T27 with the 55-kDa Tac peptide / J. Szöllösi, S. Damjanovich, C. K. Goldman, M. J. Fulwyler, A. A. Aszalos, G. Goldstein, P. Rao, M. A. Talle, T. A. Waldmann
Dátum:1987
Megjegyzések:Two monoclonal antibodies (OKT27 and OKT27b) have been produced that react with distinct epitopes of a 95-kDa peptide. The T27 antigen is widely distributed, being expressed on B lymphocytes, monocytes, and adult T-leukemic cells but not on polymorphonuclear leukocytes or platelets. There was a low level of T27 expression on resting T cells that increased on T-cell activation. In preliminary studies, the OKT27b antibody coprecipitated a 55-kDa peptide, as well as the 95-kDa peptide, from the radiolabeled cells of the HuT 102B2 cell line. Preclearance with anti-Tac, a monoclonal antibody to the 55-kDa peptide of the multichain interleukin 2 receptor, removed the 55-kDa but not the 95-kDa peptide from subsequent OKT27b immunoprecipitates of HuT 102B2 extracts, suggesting the possibility that the T27 peptide was associated with the Tac peptide. However, the precipitation of the p55 Tac peptide by OKT27b was quite inconsistent. Thus, additional information was sought using a flow cytometric energy transfer technique to provide a physical estimation of the proximity between the Tac and the T27 peptides. The flow cytometric version of the fluorescence resonance energy transfer technique permits the determination of inter- and intramolecular distances at 2- to 10-nm levels on a cell-by-cell basis. Using this approach, there was a mean energy transfer of 7.3% with HuT 102B2 cells when fluorescein isothiocyanate anti-Tac served as the donor and tetramethylrhodamine isothiocyanate OKT27 served as the acceptor. In contrast, there was no energy transfer in comparable studies observed when fluorescein anti-Tac and rhodamine anti-transferrin receptor antibodies were used. These observations support the conclusion that there is a close nonrandom proximity in HuT 102B2 cells between the 95-kDa peptide identified by the OKT27 monoclonal antibody and the p55 Tac peptide of the multichain interleukin 2 receptor.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Adult
analysis
Antibodies,Monoclonal
Antigens,CD27
Antigens,Neoplasm
Antigens,Surface
B-Lymphocytes
Biophysics
Cell Line
Energy Transfer
Epitopes
Flow Cytometry
Fluorescence
Human
Hungary
immunology
Interleukin-2
Lymphocytes
Monocytes
Peptides
Receptors,Immunologic
Receptors,Interleukin-2
Support,Non-U.S.Gov't
Support,U.S.Gov't,Non-P.H.S.
T-Lymphocytes
Tumor Cells,Cultured
Megjelenés:Proceedings of the National Academy of Sciences of the United States of America. - 84 : 20 (1987), p. 7246-7250. -
További szerzők:Damjanovich Sándor (1936-2017) (biofizikus) Goldman, Caroline K. Fulwyler, Mack J. Aszalos Adorján Goldstein, G. Rao, P. Talle, M. A. Waldmann, Thomas A.
Internet cím:elektronikus változat
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6.

001-es BibID:BIBFORM006010
Első szerző:Szöllősi János (biofizikus)
Cím:Physical association between MHC class I and class II molecules detected on the cell surface by flow cytometric energy transfer / J. Szollosi, S. Damjanovich, M. Balazs, P. Nagy, L. Tron, M. J. Fulwyler, F. M. Brodsky
Dátum:1989
Megjegyzések:The physical association of HLA class I and class II Ag in the membranes of PGF and JY lymphoblastoid cell lines was studied using flow cytometric energy transfer. This technique measures the proximity of cell surface molecules in the nm range and provides a distribution histogram of the average proximity of molecules on each cell of a population. HLA Ag were labeled with mAb conjugated to fluorescein, serving as donor, or tetramethylrhodamine, serving as acceptor molecules. Significant fluorescence energy transfer was detected between various combinations of class I and class II molecules indicating that these molecules are within 10 nanometers of each other. Specifically, energy transfer was observed between class I molecules and DR, DQ, or DP class II HLA molecules. In addition, energy transfer between all combinations of DR, DQ, and DP molecules was observed. No transfer was observed among class I molecules or among DR or among DP molecules. Among DQ molecules, subpopulations transferred fluorescence energy to each other. The close contact measured between class I and class II Ag correlates with previous reports of cocapping and may reflect an immunologically significant interaction or the reported tendency of class I Ag to associate with other cell surface receptors, including growth factor receptors. The energy transfer between fluorescent antibodies to class II Ag suggests the existence of heterodimers formed from the different locus products, as well as possible quaternary surface interactions between alpha/beta complexes from separate loci.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Antibodies,Monoclonal
Antibody Specificity
Antigens,Surface
Binding Sites
Binding Sites,Antibody
Cell Line
Cell Line,Transformed
Cell Membrane
Energy Transfer
Flow Cytometry
Fluorescence
Histocompatibility Antigens
Histocompatibility Antigens Class I
HLA-D Antigens
Human
Hungary
immunology
Lymphocytes
metabolism
Support,Non-U.S.Gov't
Support,U.S.Gov't,Non-P.H.S.
Support,U.S.Gov't,P.H.S.
Megjelenés:The Journal of Immunology. - 143 : 1 (1989), p. 208-213. -
További szerzők:Damjanovich Sándor (1936-2017) (biofizikus) Balázs Margit (1952-) (sejtbiológus, molekuláris genetikus) Nagy P. Trón Lajos (1941-) (biofizikus) Fulwyler, Mack J. Brodsky, F. M.
Internet cím:elektronikus változat
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7.

001-es BibID:BIBFORM045740
035-os BibID:(dekdb)9630543567
Első szerző:Trón Lajos (biofizikus)
Cím:On the role of cell surface dynamics and transmembrane information transfer: cyclosporin A changes physical properties of cell membranes / L. Trón, S. Damjanovich, A. Aszalós, J. Szöllősi, Sally A. Mulhern, M. J. Fulwyler
Dátum:1986
ISBN:9630543567
Tárgyszavak:Orvostudományok Elméleti orvostudományok könyvfejezet
Enzimek
Biofizika
Membránok (biológia)
Anyagcsere
Fehérjék
Nukleinsavak
Megjelenés:Dynamics of biochemical systems: lectures presented at the FEBS advanced course and round table discussion of the IUB interest group on kinetics and mechanisms of enzymes and metabolic networks: Debrecen, Hungary, 18-24 August 1985 / ed. by S. Damjanovich, T. Keleti, L. Trón. - p. 417-441. -
További szerzők:Damjanovich Sándor (1936-2017) (biofizikus) Aszalos Adorján Szöllősi János (1953-) (biofizikus) Mulhern, Sally Fulwyler, Mack J.
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
Borító:

8.

001-es BibID:BIBFORM004890
035-os BibID:(scopus)26444597596 (wos)000232299300017
Első szerző:Vereb György (biofizikus, orvos)
Cím:Biphasic calcium response of platelet-derived growth factor stimulated glioblastoma cells is a function of cell confluence / Vereb, G., Feuerstein, B. G., Hyun, W. C., Fulwyler, M. J., Balazs, M., Szollosi, J.
Dátum:2005
ISSN:1552-4922
Megjegyzések:Previous reports have linked the spiking or two-phased character of calcium transients evoked by platelet-derived growth factor (PDGF) to the position of cells in the cell cycle without regard to cell-cell contact and communication. Because cell confluence can regulate growth factor receptor expression and dephosphorylation, we investigated the effect of cell culture confluence and cell cycle on calcium responses of PDGF-BB-stimulated A172 glioblastoma cells. METHODS: Digital imaging cytometry was used to correlate the peak and duration of calcium response with bromodeoxyuridine positivity and DNA content and with culture confluence on a cell-by-cell basis. RESULTS: In serum-starved cultures, complete two-phase calcium signals and shorter, lower spikes occurred independent of cell cycle phase. However, the confluence of cell culture seemed essential for inducing a complete response because cells in sparse cultures exhibited mostly short spikes with lower peaks or no transients at all. CONCLUSION: Because cell confluence, by virtue of cell-cell contacts, is assumed to be an important regulator of proliferation, one is tempted to speculate that in transformed cells the ability to produce stronger growth signals upon reaching confluence and facing contact inhibition could provide a proliferative advantage.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
analysis
Biophysics
Bromodeoxyuridine
Calcium
Calcium Signaling
Cell Adhesion
Cell Culture
Cell Cycle
Cells
Cells,Cultured
Dna
drug effects
genetics
Glioblastoma
Hungary
metabolism
methods
pathology
pharmacology
Platelet-Derived Growth Factor
Research
Spectrometry,Fluorescence
Support
Megjelenés:Cytometry. Part A. - 67 : 2 (2005), p. 172-179. -
További szerzők:Feuerstein, Burt G. Hyun, William C. Fulwyler, Mack J. Balázs Margit (1952-) (sejtbiológus, molekuláris genetikus) Szöllősi János (1953-) (biofizikus)
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DOI
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