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001-es BibID:BIBFORM006016
Első szerző:Szöllősi János (biofizikus)
Cím:Fluorescence energy transfer measurements on cell surfaces : a critical comparison of steady-state fluorimetric and flow cytometric methods / János Szöllősi, Lajos Trón, Sándor Damjanovich, Stephen H. Helliwell, Donna Arndt-Jovin, Thomas M. Jovin
Dátum:1984
Megjegyzések:The energy transfer efficiency E was measured between fluorescein-conjugated concanavalin A (Con A) and rhodamine-conjugated Con A bound to homogeneous tissue culture cells, the HK22 murine lymphoma cell line. Results from a flow cytometric energy transfer method (FCET) and two different steady-state fluorimeter methods were compared. The data were found to be in close agreement after careful correction of the steady-state fluorimetric measurements for contributions from dissociating ligand. The biological variability of the individual cells with respect to E was calculated using an error propagation analysis and were found to be less than the variability in the absolute amount of ligand binding per cell. FCET has a number of advantages over the fluorimetric measurements using suspensions of cells: (1) relatively labile receptor-ligand complexes can be measured; (2) the analysis can be restricted to undamaged cells by gating the data collection on the light-scattering signals; (3) heterogeneous populations of cells with respect to donor and acceptor topology can be distinguished by the correlation of E with other cellular parameters derived from additional signals or combinations thereof; and (4) the dynamics of donor-acceptor redistribution on subpopulations can be measured.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
analysis
Animal
Cell Line
Cell Membrane
Concanavalin A
Energy Transfer
Flow Cytometry
Fluorescence
Lymphoma
Mathematics
methods
Mice
Mice,Inbred Strains
Microscopy,Fluorescence
pathology
physiology
physiopathology
Support,Non-U.S.Gov't
Megjelenés:Cytometry. - 5 : 2 (1984), p. 210-216. -
További szerzők:Trón Lajos (1941-) (biofizikus) Damjanovich Sándor (1936-2017) (biofizikus) Helliwell, Stephen H. Arndt-Jovin, Donna J. Jovin, Thomas M.
Internet cím:elektronikus változat
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2.

001-es BibID:BIBFORM006021
Első szerző:Trón Lajos (biofizikus)
Cím:Flow cytometric measurement of fluorescence resonance energy transfer on cell surfaces. Quantitative evaluation of the transfer efficiency on a cell-by-cell basis / Trón, L., Szöllösi, J., Damjanovich, S., Helliwell, S. H., Arndt-Jovin, D. J., Jovin, T. M.
Dátum:1984
Megjegyzések:A method has been developed for the determination of the efficiency (E) of the fluorescence resonance energy transfer between moieties on cell surfaces by use of a computer-controlled flow cytometer capable of dual wavelength excitation. The absolute value of E may be calculated on a single-cell basis. The analysis requires the measurement of samples stained with donor and acceptor conjugated ligands alone as well as together. In model experiments HK 22 murine lymphoma cells labeled with fluorescein-conjugated concanavalin A (Con A) and/or rhodamine conjugated Con A were used to determine energy transfer histograms. Using the analytic solution to energy transfer in two dimensions, a high surface density of Con A binding sites was found that suggests that the Con A receptor sites on the cell surface are to a degree preclustered . We call this technique flow cytometric energy transfer ( FCET ).
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
analysis
Animal
Binding Sites
Cell Line
Cell Membrane
Comparative Study
Concanavalin A
Energy Transfer
Flow Cytometry
Fluorescein
Fluorescein-5-isothiocyanate
Fluoresceins
Fluorescence
Ligands
Lymphoma
metabolism
Mice
Rhodamines
Spectrometry,Fluorescence
Support,Non-U.S.Gov't
Thiocyanates
Megjelenés:Biophysical Journal. - 45 : 5 (1984), p. 939-946. -
További szerzők:Szöllősi János (1953-) (biofizikus) Damjanovich Sándor (1936-2017) (biofizikus) Helliwell, Stephen H. Arndt-Jovin, Donna J. Jovin, Thomas M.
Internet cím:elektronikus változat
Borító:
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