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001-es BibID:	BIBFORM006053
Első szerző:	Szöllősi János (biofizikus)
Cím:	Attachment of A172 human glioblastoma cells affects calcium signalling : a comparison of image cytometry, flow cytometry, and spectrofluorometry / Szollosi, J., Feuerstein, B. G., Hyun, W. C., Das, M. K., Marton, L. J.
Dátum:	1991
Megjegyzések:	The intracellular free calcium concentration ([Ca2+]i) of indo-1 loaded A172 human glioblastoma cells stimulated by platelet-derived growth factor (PDGF) was studied in cell suspensions by flow cytometry and spectrofluorometry and in confluent monolayers by laser image cytometry and spectrofluorometry. With all three techniques, the percentage of responsive cells, peak [Ca2+]i, and the duration of response were directly related, and the delay time was inversely related to PDGF dose. The maximum response occurred at a PDGF concentration of about 20 ng/ml. Basal and peak [Ca2+]i did not differ significantly from method to method even though different calibration procedures were used. Cells in suspension monitored by both spectrofluorometry and flow cytometry displayed significantly shorter calcium responses than attached cells. This did not appear to be a direct effect of trypsinization. Spectral analysis of indo-1 in cytoplasm, 40% glycerol, and aqueous solutions showed significant differences in the isosbestic point and quantum efficiency. Calibration of [Ca2+]i with spectrofluorometry is more accurate using the ratio of fluorescence intensities than the fluorescence intensities measured at either 405 or 485 nm.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk analysis Calcium Cell Separation Chelating Agents Comparative Study Cytoplasm drug therapy Dyes Flow Cytometry Fluorescence Fluorescent Dyes Glioblastoma Glioma Glycerol Human Image Cytometry Indoles metabolism pharmacology Platelet-Derived Growth Factor Spectrometry,Fluorescence Support,Non-U.S.Gov't Support,U.S.Gov't,P.H.S. Tumor Cells,Cultured
Megjelenés:	Cytometry. - 12 : 8 (1991), p. 707-716. -
További szerzők:	Feuerstein, Burt G. Hyun, William C. Das, M. K. Marton, Laurence J.
Internet cím:	elektronikus változat
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