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001-es BibID:	BIBFORM001290
035-os BibID:	(Wos)000187210800003 (Scopus)0348010590
Első szerző:	Röszer Tamás (orvos, biológus)
Cím:	A possible stimulatory effect of FMRFamide on neural nitric oxide production in the central nervous system of Helix lucorum L. / Tamás Röszer, Zsolt Jenei, Tamás Gáll, Olivér Nagy, Zsolt Czimmerer, Zoltán Serfözö, Károly Elekes, Gáspár Bánfalvi
Dátum:	2004
Megjegyzések:	The anatomical and functional relationship between neurons expressing nitric oxide (NO) synthase and molluscan cardioexcitatory (FMRFamide)-like neuropeptides was studied in the central ganglia of Helix lucorum (Pulmonata, Gastropoda), applying NADPHdiaphorase (NADPHd) histochemistry to visualize NO synthase and immunocytochemistry to demonstrate FMRFamide (FMRFa) at the light microscopic level. The NO production of the ganglia was detected by the colorimetric Griess determination of nitrite, a breakdown product of NO. Effects of the NO synthase substrate amino acid L-arginine, the NO synthase inhibitor Nomega-nitro-L-arginine (NOARG), synthetic FMRFa and the FMRFa sensitive ion channel blocker amiloride hydrochloride on nitrite production were also tested. NADPHd reaction labeled nerve cells and fibers in the procerebra, mesocerebra and metacerebra within the cerebral ganglia, and cell clusters in the postcerebral ganglia. FMRFa immunolabeling could be observed within subpopulations of NADPHd positive cells and in pericellular varicose fibers surrounding NADPHd stained neurons. Nitrite production of the ganglia was stimulated by L-arginine (10- 20 mM) but was decreased by NOARG (1-2 mM). Synthetic FMRFa (0.830-3.340 mM) increased the nitrite production in a dose dependent manner, but was ineffective in the presence of NOARG. Amiloride hydrochloride (7.890 mM) reduced the FMRFa evoked nitrite production in all ganglia. This is the first description of an anatomical relationship between putative NO producing and FMRFa containing cells, suggesting a possible regulatory role of FMRFa in the NO mediated signaling in an invertebrate nervous system. Copyright 2004 S. Karger AG, Basel
Tárgyszavak:	Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk FMRFamid nitrogén monoxid neurokémia gerinctelenek fejlábúak éticsiga
Megjelenés:	Brain, Behavior and Evolution. - 63 : 1 (2004), p.23-33. -
További szerzők:	Jenei Zsolt Gáll Tamás Nagy Olivér Czimmerer Zsolt (1981-) (molekuláris biológus) Serfőző Zoltán Elekes Károly Bánfalvi Gáspár (1943-) (sejtbiológus, gyógyszerész)
Internet cím:	elektronikus változat
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001-es BibID:	BIBFORM043618
035-os BibID:	(Wos)000183199400003 (Scopus)0038045730
Első szerző:	Serfőző Zoltán
Cím:	Development of the nitric oxide/cGMP system in the embryonic and juvenile pond snail, Lymnaea stagnalis L. A comparative in situ hybridization, histochemical and immunohistochemical study / Zoltán Serfőző, Zoltán Veréb, Tamás Rőszer, György Kemenes, Károly Elekes
Dátum:	2002
ISSN:	0030-6002 1788-6120
Megjegyzések:	AbstractRecent studies have indicated that nitric oxide (NO)-induced cGMP synthesis is involved in different steps of neurogenesis in invertebrates. The development of putative NO synthetising elements was described earlier in the embryonic and juvenile pond snail, Lymnaea stagnalis, applying NADPH-diaphorase histochemistry (Serfozo et al., 1998). In the present study, we examined the distribution of NO synthase (NOS) during Lymnaea development by in situ hybridization for Lymnaea-NOS mRNA, histochemical, and immunohistochemical techniques for the NOS and NO-stimulated cGMP. Peripheral fibers projecting to the CNS and terminating in the ganglionic neuropils showed NOS immunoreactivity from 85% of embryonic development. At the same time, a fine dot-like, immunostaining indicated the presence of cGMP in the neuropil area. In the CNS, Lymnaea-NOS mRNA positive, as well as NOS and cGMP immunoreactive perikarya were detected first during postembryonic development; their number significantly increased from P3 juvenile stage. Some of the cell groups in the CNS containing NOS immunoreactive material also displayed Lymnaea-NOS mRNA hybridization signal and were cGMP-positive. However, in the subesophageal ganglia, the distribution of Lymnaea-NOS mRNA positive cell groups did not correspond to that of the NOS immunoreactive cells. Neurons revealing transient NOS and cGMP immunoreactivity, respectively, could also be detected in this part of the CNS. In most of the ganglia the number of Lymnaea-NOS mRNA containing and cGMP immunopositive neurons, respectively, exceeded that of the NOS immunoreactive cells from P4 juvenile stage. The localization of NADPH-diaphorase reaction also correlated well with that of the NOS immunoreactivity in the developing CNS. At the periphery, colocalization of Lymnaea-NOS mRNA signal, NOS and cGMP immunoreactivities were observed in the epithelial cells of the esophagus and mantle after hatching. The findings suggest the functional maturity of the NO/cGMP signal transduction pathway at both central and peripheral levels during the development of the snail, Lymnaea stagnalis. The differences in the localization of Lymnaea-NOS mRNA labeling and NOS immunoreactivity in the CNS and PNS can be explained by the existence of different NOS isoforms, posttranslational regulation of NOS, and/or some non-specific antibody labeling.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk Stem cell therapy Buerger PAD
Megjelenés:	Journal of Neurocytology. - 31 : 2 (2002), p. 131-147. -
További szerzők:	Veréb Zoltán (1980-) (immunológus, mikrobiológus, molekuláris biológus) Röszer Tamás (1979-) (orvos, biológus) Kemenes György Elekes Károly
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat DOI
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