CCL

Összesen 1 találat.
#/oldal:
Részletezés:
Rendezés:

1.

001-es BibID:BIBFORM087405
035-os BibID:(cikkazonosító)5135 (WoS)000554266900001 (Scopus)85088162016
Első szerző:Kotogány Edit
Cím:Imidazo[1,2-b]pyrazole-7-Carboxamide Derivative Induces Differentiation-Coupled Apoptosis of Immature Myeloid Cells Such as Acute Myeloid Leukemia and Myeloid-Derived Suppressor Cells / Edit Kotogány, József Á. Balog, Lajos I. Nagy, Róbert Alföldi, Valeria Bertagnolo, Federica Brugnol, András Demjén, Anita K. Kovács, Péter Batár, Gabriella Mezei, Renáta Szabó, Iván Kanizsai, Csaba Varga, László G. Puskás, Gábor J. Szeben
Dátum:2020
ISSN:1422-0067
Megjegyzések:Chemotherapy-induced differentiation of immature myeloid progenitors, such as acute myeloid leukemia (AML) cells or myeloid-derived suppressor cells (MDSCs), has remained a challenge for the clinicians. Testing our imidazo[1,2-b]pyrazole-7-carboxamide derivative on HL-60 cells, we obtained ERK phosphorylation as an early survival response to treatment followed by the increase of the percentage of the Bcl-xlbright and pAktbright cells. Following the induction of Vav1 and the AP-1 complex, a driver of cellular differentiation, FOS, JUN, JUNB, and JUND were elevated on a concentration and time-dependent manner. As a proof of granulocytic differentiation, the cells remained non-adherent, the expression of CD33 decreased; the granularity, CD11b expression, and MPO activity of HL-60 cells increased upon treatment. Finally, viability of HL-60 cells was hampered shown by the depolarization of mitochondria, activation of caspase-3, cleavage of Z-DEVD-aLUC, appearance of the sub-G1 population, and the leakage of the lactate-dehydrogenase into the supernatant. We confirmed the differentiating effect of our drug candidate on human patient-derived AML cells shown by the increase of CD11b and decrease of CD33+, CD7+, CD206+, and CD38bright cells followed apoptosis (IC50: 80 nM) after treatment ex vivo. Our compound reduced both CD11b+/Ly6C+ and CD11b+/Ly6G+ splenic MDSCs from the murine 4T1 breast cancer model ex vivo.
Tárgyszavak:Orvostudományok Klinikai orvostudományok kutatási jelentés
folyóiratcikk
acute myeloid leukemia
myeloid-derived suppressor cells
differentiation
apoptosis
Megjelenés:International Journal Molecular Sciences. - 21 : 14 (2020), p. 1-27. -
További szerzők:Balog József A. Nagy Lajos István (Szeged) Alföldi Róbert Bertagnolo, Valeria Brugnol, Federica Demjén András Kovács Anita K. Batár Péter (1969-) (belgyógyász, haematológus) Mezei Gabriella (1969-) (belgyógyász) Szabó Renáta Kanizsai Iván Varga Csaba (orvos) Puskás László G. Szeben Gábor J.
Internet cím:DOI
Intézményi repozitóriumban (DEA) tárolt változat
Borító:
Rekordok letöltése1