Találati lista | Tudóstér

001-es BibID:	BIBFORM016328
Első szerző:	Maródi László (gyermekgyógyász infektológus, immunológus)
Cím:	Augmentation of Human Macrophage Candidacidal Capacity by Recombinant Human Myeloperoxidase and Granulocyte- Macrophage Colony-Stimulating Factor / Maródi László, Tournay Christophe, Káposzta Rita, Richard B. Johnston J.R., Nicole Moguilevsky
Dátum:	1998
Megjegyzések:	Phagocyte myeloperoxidase (MPO) is believed to be particularly important in defense against candida infection. We reported earlier that monocytes, rich in MPO, killed Candida albicans at a significantly higher rate and extent than did monocyte-derived macrophages, known to lack MPO, and that C. albicans is less resistant to MPO-dependent oxidants than less pathogenic Candida species. We hypothesized, therefore, that the capacity of macrophages to kill C. albicans might be improved in the presence of MPO. In this study, we evaluated the ability of recombinant human MPO (rhMPO) to augment the killing of C. albicans by resident macrophages and macrophages activated by recombinant human granulocyte-macrophage colony-stimulating factor. Addition of rhMPO (concentration range, 0.8 to 6.4 U/ml) to suspensions of resident and activated macrophages and opsonized C. albicans resulted in concentration-dependent and significant increases in candida killing. This enhancement was particularly pronounced with activated macrophages, whether C. albicans was opsonized or unopsonized and ingested through the macrophage mannose receptor. rhMPO did not affect the killing of C. albicans by monocytes, nor did it affect phagocytosis of opsonized or unopsonized C. albicans. These results indicate that exogenous rhMPO can augment the candidacidal capacity of both resident and activated macrophages, with a more profound effect on activated cells. We suggest that rhMPO may be effective in the treatment of invasive candidiasis.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	Infection and Immunity. - 66 : 6 (1998), p. 2750-2754. -
További szerzők:	Christophe, Tournay Káposzta Rita (1968-) (csecsemő- és gyermekgyógyász) Johnston, Richard B. jr. Moguilevsky, Nicole
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat elektronikus változat
Borító:
Saját polcon:

001-es BibID:	BIBFORM018081
Első szerző:	Tournay, Christophe
Cím:	Uptake of recombinant myeloperoxidase, free or fused to Fc gamma, by macrophages enhances killing activity toward micro-organisms / Tournay Christophe, Courtoy J. Pierre, Maródi László, Totté Philippe, Werenne John, Jacquet Alain, Garcia-Quintana Lida, Bollen Alex, Moguilevsky Nicole
Dátum:	1996
Megjegyzések:	A chimeric antibody-like molecule consisting of the human myeloperoxidase (rMPO) fused to the second and third constant-sequence (CH2 and CH3) Fc domains of human immunoglobulin G-1 has been constructed and expressed in Chinese hamster ovary (CHO) cells. This fusion molecule was designed to combine the binding specificity of Fc with the antimicrobial properties of rMPO. The rMPO-Fc fusion dimerized through the Fc fragment, while retaining the enzymatic activity of rMPO. The chimeric molecule was glycosylated and most of the propeptide was eliminated, indicating a better processing of the polypeptide than for rMPO alone. Both rMPO and rMPO-Fc bound to and were internalized by macrophage-like U937 promonocytic cells. Unexpectedly, the chimera failed to bind to the Fc receptor but interacted with a higher affinity than rMPO with the same binding sites. The presence of the Fc fragment in the chimera, in addition, did not extend the plasma half-life of the fusion protein. In vitro, rMPO-Fc exhibited a stronger killing effect than rMPO toward Candida albicans in the presence of either H202 alone or human macrophages. In vivo, rMPO-Fc similarly conferred a better protection than rMPO in a lethal model of murine cowdriosis. These properties could be related to the Fc-induced dimerization of the fusion protein in CHO cells.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	DNA and Cell Biology. - 15 : 8 (1996), p. 617-624. -
További szerzők:	Courtoy, J. Pierre Maródi László (1949-) (gyermekgyógyász infektológus, immunológus) Totté, Philippe Werenne, John Jacquet, Alain Garcia-Quintana, Lida Bollen, Alex Moguilevsky, Nicole
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat
Borító:
Saját polcon: