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001-es BibID:	BIBFORM004875
Első szerző:	Márián Teréz (radiobiológus)
Cím:	Effects of miltefosine on membrane permeability and accumulation of [99mTc]-hexakis-2-methoxyisobutyl isonitrile, 2-[18F]fluoro-2-deoxy-D-glucose, daunorubucin and rhodamine123 in multidrug-resistant and sensitive cells / Marian, T., Balkay, L., Tron, L., Krasznai, Z. T., Szabo-Peli, J., Krasznai, Z.
Dátum:	2005
ISSN:	0928-0987
Megjegyzések:	Miltefosine is a phospholipid analog that exhibits antineoplastic activity against breast cancer metastases, but its mechanism of action remains uncertain. The aim of this study was to investigate the transport mechanism for the removal of miltefosine and [99mTc]-hexakis-2-methoxyisobutyl isonitrile (99mTc-MIBI) from multidrug-resistant cells. The P-glycoprotein pump function, cell viability, and 99mTc-MIBI and 2-[18F]fluoro-2-deoxy-D-glucose (18FDG) uptakes were measured in NIH 3T3 (3T3) and NIH 3T3MDR1 G185 (3T3MDR1) mouse fibroblasts and human lymphoid B JY cells. Miltefosine treatment increased the permeability and fluidity of these tumor cells in a concentration-dependent manner. The multidrug-sensitive cells were 3-4 times more sensitive to miltefosine than the multidrug-resistant ones. The extent of 99mTc-MIBI accumulation in the P-glycoprotein-expressing cells increased in the presence of miltefosine, whereas the rhodamine123 and daunorubicin uptakes of the cells did not change significantly. In the 3T3MDR1 cells verapamil reinstated the rhodamine123 and daunorubicin accumulation, but not the 99mTc-MIBI uptake. Cyclosporin A reinstated the uptakes of 99mTc-MIBI, daunorubicin and rhodamine123 by the 3T3MDR1 cells. In a concentration-dependent manner miltefosine decreased the extents of 99mTc-MIBI, rhodamine123, daunorubicin and 18FDG accumulation in the JY and 3T3 cells. Our findings indicate a common transport mechanism for 99mTc-MIBI and miltefosine, which is distinct from that for rhodamine123 and daunorubicin in MDR cells
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban 3T3 Cells analogs & derivatives analysis Animals Cell Membrane Permeability Cells Daunorubicin drug effects Drug Resistance,Multiple Fibroblasts Fluorodeoxyglucose F18 Human Humans Hungary Membrane Fluidity Mice Nih 3T3 Cells P-Glycoprotein Permeability pharmacokinetics pharmacology Phosphorylcholine Research Rhodamine 123 Support Technetium Tc 99m Sestamibi Verapamil
Megjelenés:	European Journal of Pharmaceutical Sciences. - 24 : 5 (2005), p. 495-501. -
További szerzők:	Balkay László (1963-) (biofizikus) Trón Lajos (1941-) (biofizikus) Krasznai Zoárd Tibor (1973-) (szülész-nőgyógyász, gyermeknőgyógyász) Szabó-Péli Judit (1977-) (kutató) Krasznai Zoltán (1950-) (biofizikus)
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001-es BibID:	BIBFORM004874
035-os BibID:	(scopus)21144454228 (wos)000229662400002
Első szerző:	Márián Teréz (radiobiológus)
Cím:	Biphasic accumulation kinetics of [99mTc]-hexakis-2-methoxyisobutyl isonitrile in tumour cells and its modulation by lipophilic P-glycoprotein ligands / Marian, T., Balkay, L., Szabo, G., Krasznai, Z. T., Hernadi, Z., Galuska, L., Szabo-Peli, J., Esik, O., Tron, L., Krasznai, Z.
Dátum:	2005
ISSN:	0928-0987
Megjegyzések:	To study the accumulation and washout kinetics of [99mTc]-hexakis-2-methoxyisobutyl isonitrile (99mTc-MIBI) in MDR positive and MDR negative tumour cells and how this is modified by lipophilic P-glycoprotein ligands. METHODS: The tumour cells were incubated in the presence and absence of the ligands and the uptakes of 99mTc-MIBI, rhodamine 123 and 2-[18F]fluoro-2-deoxy-D-glucose (18FDG) were measured. RESULTS: The accumulation of 99mTc-MIBI in the tumour cells followed biphasic kinetics. Verapamil and cyclosporin A increased the membrane fluidity and significantly enhanced the 99mTc-MIBI uptake of the MDR negative cells, while the rhodamine 123 uptake was not affected. Verapamil significantly increased the uptake of rhodamine 123 and 18FDG but did not modify that of 99mTc-MIBI in the MDR positive cells. Cyclosporin A significantly increased the 18FDG uptake of the MDR positive and negative tumour cells; these effects were ouabain-sensitive. Depolarization of the cytoplasmic membrane, acidification of the extracellular medium and the administration of CCCP decreased the accumulation of 99mTc-MIBI and rhodamine 123 uptake in the tumour cells. CONCLUSIONS: Lipophilic P-glycoprotein ligands modified the biphasic accumulation kinetics of the 99mTc-MIBI uptakes of MDR negative and positive tumour cells in different and complex ways and could therefore mask the P-glycoprotein pump-dependent changes in tracer accumulation.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk Animals Cell Line,Tumor Cells Cricetinae Cyclosporine drug effects Drug Resistance,Multiple Fluorine Fluorine Radioisotopes Gluconates Humans Hungary Intracellular Membranes Kinetics Ligands Membrane Fluidity Membrane Potentials metabolism methods Mice Mitochondria P-Glycoprotein Permeability pharmacokinetics pharmacology physiology Radioisotopes Research Rhodamine 123 Support Technetium Tc 99m Sestamibi Verapamil
Megjelenés:	European Journal of Pharmaceutical Sciences. - 25 : 2-3 (2005), p. 201-209. -
További szerzők:	Balkay László (1963-) (biofizikus) Szabó Gábor (1953-) (biofizikus) Krasznai Zoárd Tibor (1973-) (szülész-nőgyógyász, gyermeknőgyógyász) Hernádi Zoltán (1948-) (szülész-nőgyógyász, klinikai onkológus) Galuska László (1946-) (belgyógyász, izotópdiagnoszta) Szabó-Péli Judit (1977-) (kutató) Ésik Olga Trón Lajos (1941-) (biofizikus) Krasznai Zoltán (1950-) (biofizikus)
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