Találati lista | Tudóstér

001-es BibID:	BIBFORM040367
Első szerző:	Cayli, Sevil
Cím:	Biochemical markers of sperm function : male fertility and sperm selection for ICSI / Cayli, S., Jakab, A., Ovari, L., Delpiano, E., Celik-Ozenci, C., Sakkas, D., Ward, D., Huszar, G.
Dátum:	2003
ISSN:	1472-6483
Megjegyzések:	The expression of a 70 kDa chaperone protein, HspA2 (formerly called CK-M), has been identified in mature human spermatozoa. The central role of HspA2 has been established, as the expression level of this protein is related to sperm cellular maturity, DNA integrity, chromatin maturity, chromosomal aneuploidy frequency and sperm function, including fertilizing potential. The spermiogenetic events of cytoplasmic extrusion and remodelling of the plasma membrane, which facilitate the formation of zona pellucida binding site(s) in human spermatozoa, are related. Finally, the presence of the hyaluronic acid (HA) receptor on the plasma membrane of mature sperm coupled with the HA-coated slide sperm-binding assay, facilitates the testing of infertile men and the selection of single mature spermatozoa for ICSI. Because mature spermatozoa have no residual cytoplasm, the HA-bound sperm fraction is also enriched in spermatozoa that are normal by the Kruger strict morphology method.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	Reproductive Biomedicine Online. - 7 : 4 (2003), p. 462-468. -
További szerzők:	Jakab Attila (1964-) (szülész-nőgyógyász, endokrinológus) Óvári László (szülész-nőgyógyász, sebész) Delpiano, Elena Celik-Ozenci, Ciler Sakkas, Denny Ward, D. Huszár Gábor
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
Borító:
Saját polcon:

001-es BibID:	BIBFORM023079
Első szerző:	Huszár Gábor
Cím:	Hyaluronic acid binding ability of human sperm reflects cellular maturity and fertilizing potential : selection of sperm for intracytoplasmic sperm injection / Huszár G., Ozkavukcu S., Jakab A., Celik-Ozenci C., Sati L. G., Cayli S.
Dátum:	2006
ISSN:	1040-872X
Megjegyzések:	The current concepts of sperm biochemical markers and the central role of the HspA2 chaperone protein, a measure of sperm cellular maturity and fertilizing potential, are reviewed. RECENT FINDINGS: Because HspA2 is a component of the synaptonemal complex, low HspA2 levels and increased frequency of chromosomal aneuploidies are related in diminished maturity sperm. We also suggest a relationship between HspA2 expression in elongating spermatids and events of late spermiogenesis, such as cytoplasmic extrusion and plasma membrane remodeling that aid the formation of the zona pellucida binding and hyaluronic acid binding sites. The presence of hyaluronic acid receptor on the plasma membrane of mature sperm, coupled with hyaluronic acid coated glass or plastic surfaces, facilitates testing of sperm function and selection of single mature sperm for intracytoplasmic sperm injection. The frequencies of sperm with chromosomal disomy are reduced approximately fourfold to fivefold in hyaluronic acid selected sperm compared with semen sperm, comparable to the increase in such abnormalities in intracytoplasmic sperm injection offspring. Hyaluronic acid binding also excludes immature sperm with cytoplasmic extrusion, persistent histones, and DNA chain breaks. SUMMARY: Hyaluronic acid mediated sperm selection is a novel technique that is comparable to sperm zona pellucida binding. Hyaluronic acid selected sperm will also alleviate the risks related to intracytoplasmic sperm injection fertilization with sperm of diminished maturity that currently cause worldwide concern.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban chromosomal disomy and diploidy intracytoplasmic sperm injection sperm maturation zona pellucida binding
Megjelenés:	Current Opinion In Obstetrics & Gynecology. - 18 : 3 (2006), p. 260-267. -
További szerzők:	Ozkavukcu, Sinan Jakab Attila (1964-) (szülész-nőgyógyász, endokrinológus) Celik-Ozenci, Ciler Sati, Leyla G. Cayli, Sevil
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat DOI
Borító:
Saját polcon:

001-es BibID:	BIBFORM002256
Első szerző:	Huszár Gábor
Cím:	Fertility testing and ICSI sperm selection by hyaluronic acid binding : clinical and genetic aspects / Gabor Huszar, Attila Jakab, Denny Sakkas, Ciler-Celik Ozenci, Sevil Cayli, Elena Delpiano, Sinan Ozkavukcu
Dátum:	2007
Megjegyzések:	The testis-expressed chaperone protein, HspA2 (previously creatine kinase M isoform) was established as a measure of human sperm cellular maturity, function and fertility. The presence of HspA2 in the synaptonemal complex is likely to link low HspA2 expression and increased frequency of chromosomal aneuploidies in arrested-maturity spermatozoa. A relationship also exists between HspA2 expression in elongating spermatids and the associated spermatogenetic events, including plasma membrane remodelling and the formation of zona pellucida and hyaluronic acid (HA) binding sites. The HA receptor of mature spermatozoa, when coupled with HA-coated slides and/or Petri dishes, allows visual observation of sperm-HA binding, providing a basis for sperm maturity testing, a major improvement in semen evaluation, and selection of mature spermatozoa for intracytoplasmic sperm injection (ICSI). Thus, in HA-selected spermatozoa the frequency of chromosomal disomy and diploidy is reduced 4- to 6-fold compared with semen sperm fractions. This reduction is similar to the increase in numerical chromosomal aberrations in ICSI children. Combined studies of sperm shape and chromosome probes demonstrated that sperm morphology does not aid selection of haploid spermatozoa. The HA-mediated sperm selection is a novel and efficient technique that may alleviate potential problems related to ICSI fertilization with visually selected spermatozoa.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	Reproductive Biomedicine Online 14 : 5 (2007), p. 650-663. -
További szerzők:	Jakab Attila (1964-) (szülész-nőgyógyász, endokrinológus) Sakkas, Denny Ozenci, Ciler-Celik Cayli, Sevil Delpiano, Elena Ozkavukcu, Sinan
Internet cím:	elektronikus változat
Borító:
Saját polcon:

001-es BibID:	BIBFORM023077
Első szerző:	Jakab Attila (szülész-nőgyógyász, endokrinológus)
Cím:	Intracytoplasmic sperm injection : a novel selection method for sperm with normal frequency chromosomal aneuploidies / Jakab A., Sakkas D., Delpiano E., Cayli S., Kovanci E., Ward D., Revelli A., Huszár G.
Dátum:	2005
ISSN:	0015-0282
Megjegyzések:	To test a newly invented intracytoplasmic sperm injection (ICSI) sperm selection method based on sperm hyaluronic acid (HA) binding. Design: Comparison of chromosomal disomy and diploidy frequencies in sperm arising from semen and in HA-bound sperm. Setting: Academic andrology laboratory. Patient(s): Men presenting for semen analysis. Intervention(s): Washed sperm fractions of 32 semen samples were applied to Petri dishes or glass slides coated with immobilized HA. The unbound sperm were rinsed gently, and the HA-bound sperm were removed with an ICSI pipette. The control sperm population was the unselected sperm. Both HA-selected and unselected sperm were treated with fluorescence in situ hybridization with centromeric probes for the X, Y, and 17 chromosomes. Main Outcome Measure(s): Chromosomal disomy and diploidy frequencies. Result(s): In the HA-bound sperm (495?2,079 per man, 41,670 in all) compared with unselected sperm (4,770 per man, 162,210 in all), the chromosomal disomy frequencies were reduced to 0.16% from 0.52%, diploidy to 0.09% from 0.51%, and sex chromosome disomy to 0.05% from 0.27% (a 5.4-fold reduction vs. 4-fold respective increase in ICSI offspring). Conclusion(s): The HA sperm selection method for ICSI, which is based on a relationship between sperm receptors for zona pellucida and HA, will likely reduce the potential genetic complications and adverse public health effects of ICSI.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban Sperm maturity chromosomal aneuploidies ICSI sperm selection genetic integrity paternal contribution
Megjelenés:	Fertility And Sterility. - 84 : 6 (2005), p. 1665-1673. -
További szerzők:	Sakkas, Denny Delpiano, Elena Cayli, Sevil Kovanci, Ertug Ward, David Revelli, Alberto Huszár Gábor
Internet cím:	DOI Intézményi repozitóriumban (DEA) tárolt változat
Borító:
Saját polcon: