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1.

001-es BibID:BIBFORM040367
Első szerző:Cayli, Sevil
Cím:Biochemical markers of sperm function : male fertility and sperm selection for ICSI / Cayli, S., Jakab, A., Ovari, L., Delpiano, E., Celik-Ozenci, C., Sakkas, D., Ward, D., Huszar, G.
Dátum:2003
ISSN:1472-6483
Megjegyzések:The expression of a 70 kDa chaperone protein, HspA2 (formerly called CK-M), has been identified in mature human spermatozoa. The central role of HspA2 has been established, as the expression level of this protein is related to sperm cellular maturity, DNA integrity, chromatin maturity, chromosomal aneuploidy frequency and sperm function, including fertilizing potential. The spermiogenetic events of cytoplasmic extrusion and remodelling of the plasma membrane, which facilitate the formation of zona pellucida binding site(s) in human spermatozoa, are related. Finally, the presence of the hyaluronic acid (HA) receptor on the plasma membrane of mature sperm coupled with the HA-coated slide sperm-binding assay, facilitates the testing of infertile men and the selection of single mature spermatozoa for ICSI. Because mature spermatozoa have no residual cytoplasm, the HA-bound sperm fraction is also enriched in spermatozoa that are normal by the Kruger strict morphology method.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Reproductive Biomedicine Online. - 7 : 4 (2003), p. 462-468. -
További szerzők:Jakab Attila (1964-) (szülész-nőgyógyász, endokrinológus) Óvári László (szülész-nőgyógyász, sebész) Delpiano, Elena Celik-Ozenci, Ciler Sakkas, Denny Ward, D. Huszár Gábor
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2.

001-es BibID:BIBFORM038980
Első szerző:Celik-Ozenci, Ciler
Cím:Mature and fertile sperm selectively bind to hyaluronic acid : cytoplasmic content, HspA2 levels, chromatin maturity, shape and ICSI sperm selection / Celik-Ozenci C., Jakab A., Vigue L., Demir R., Huszar G.
Dátum:2002
ISSN:1071-5576
Tárgyszavak:Orvostudományok Klinikai orvostudományok idézhető absztrakt
külföldön készült közlemény
Megjelenés:Journal of The Society for Gynecologic Investigation 9 (2002), p. 849. -
További szerzők:Jakab Attila (1964-) (szülész-nőgyógyász, endokrinológus) Vigue, Lynne Demir, Ramazan Huszár Gábor
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3.

001-es BibID:BIBFORM038987
Első szerző:Celik-Ozenci, Ciler
Cím:Can one visually select aneuploidy-free sperm : a study of morphometry and fluorescence in-situ hybridization / Celik-Ozenci C. Catalanotti J., Jakab A. Kovacs T. Demir R. Huszar G.
Dátum:2002
ISSN:0268-1161
Tárgyszavak:Orvostudományok Klinikai orvostudományok idézhető absztrakt
külföldön készült közlemény
Megjelenés:Human Reproduction 17 : Suppl. 1. (2002), p. 41. -
További szerzők:Catalanotti, Jillian Jakab Attila (1964-) (szülész-nőgyógyász, endokrinológus) Kovács Tamás (1959-) (szülész-nőgyógyász, humángenetikus) Demir, Ramazan Huszár Gábor
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4.

001-es BibID:BIBFORM023074
Első szerző:Celik-Ozenci, Ciler
Cím:Sperm selection for ICSI : shape properties do not predict the absence or presence of numerical chromosomal aberrations / Ciler Celik-Ozenci, Attila Jakab, Tamas Kovacs, Jillian Catalanotti, Ramazan Demir, Patricia Bray-Ward, David Ward, Gabor Huszar
Dátum:2004
ISSN:0268-1161
Megjegyzések:We hypothesize that the potential relationship between abnormal sperm morphology and increased frequency of numerical chromosomal aberrations is based on two attributes of diminished sperm maturity: (i) cytoplasmic retention and consequential sperm shape abnormalities; and (ii) meiotic errors caused by low levels of the HspA2 chaperone, a component of the synaptonemal complex. Because sperm morphology and aneuploidies were assessed in semen, but not in the same spermatozoa, previous studies addressing this relationship were inconclusive. We recently demonstrated that sperm shape is preserved following fluorescence in situ hybridization (FISH). Thus, we examined the shape and chromosomal aberrations in the same sperm. METHODS: We performed phase contrast microscopy and FISH, using centromeric probes for chromosomes X, Y, 10, 11 and 17 in 15 men. The fluorescence and respective phase contrast images were digitized using the Metamorph program. We studied 1286 sperm (256 disomic, 130 diploid and 900 haploid sperm) by three criteria: head and tail dimensions, head shape and Kruger strict morphology. Furthermore, in each analysis, we considered whether disomic or diploid sperm may be distinguished from haploid sperm. RESULTS: There was an overall, but not discriminative, relationship between abnormal sperm dimensions or shape and increased frequencies of numerical chromosomal aberrations. However, approximately 68 of the 256 disomic, and four of 130 diploid sperm showed head and tail dimensions comparable with the most normal, lowest tertile of the 900 haploid spermatozoa. Considering all 1286 sperm, among those with the most regular, symmetrical shape (n = 367), there were 63 and five with disomic and diploid nuclei, respectively. In line with these findings, among the 256 disomic sperm, 10% were Kruger normal. CONCLUSIONS: Sperm dimensions or shape are not reliable attributes in selection of haploid sperm for ICSI.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
spermium aneuploidia
Megjelenés:Human Reproduction. - 19 : 9 (2004), p. 2052-2059. -
További szerzők:Jakab Attila (1964-) (szülész-nőgyógyász, endokrinológus) Kovács Tamás (1959-) (szülész-nőgyógyász, humángenetikus) Catalanotti, Jillian Demir, Ramazan Bray-Ward, Patricia Ward, David Huszár Gábor
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5.

001-es BibID:BIBFORM023072
Első szerző:Celik-Ozenci, Ciler
Cím:Human Sperm Maintain Their Shape Following Decondensation and Denaturation for Fluorescent In Situ Hybridization : shape Analysis and Objective Morphometry / Celik-Ozenci C., Catalanotti J., Jakab A., Aksu C., Ward D., Bray-Ward P., Demir R., Huszar G.
Dátum:2003
ISSN:0006-3363
Megjegyzések:The relationship between abnormal sperm morphology and chromosomal aberrations has been of interest. Thus far, however, studies have focused on frequencies of sperm with either abnormal morphology or aneuploidies in semen samples, not on detection of individual spermatozoa exhibiting both abnormal morphology and aneuploidy. To assess the feasibility of simultaneous evaluation of both attributes in an individual sperm cell, we investigated whether sperm shape is preserved after decondensation and denaturation, procedures that are required for fluorescent in situ hybridization (FISH). On 21 slides, 395 sperm were fixed, photographed, and then digitized by the computer-assisted Metamorph morphometry program for individual evaluation before decondensation. To establish whether sperm of various shapes would behave in similar manners, the cells were also classified, according to their head shapes, into symmetrical (n = 115), asymmetrical (n = 115), irregular (n = 115), and amorphous (n = 50) categories. Following decondensation and subsequent denaturation, sperm that had been photographed initially were relocalized and digitized for morphometry. Head area, perimeter, long axis, short axis, shape factor, and tail length were evaluated in each of the 395 sperm in both the native and decondensed states. After the decondensation and denaturation protocol of the FISH procedure, the sperm exhibited a proportional increase in dimensions as compared to their original sizes. Their initial shapes were preserved with high fidelity whether the sperm were in the symmetrical, asymmetrical, irregular, or amorphous categories. Hybridization with the chromosome probes had no further effect on sperm shape or size. We provide images to demonstrate how these findings facilitate studies about the relationship between sperm shape and chromosomal content or aberrations in individual spermatozoa.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
sperm
Megjelenés:Biology of Reproduction. - 69 : 4 (2003), p. 1347-1355. -
További szerzők:Catalanotti, Jillian Jakab Attila (1964-) (szülész-nőgyógyász, endokrinológus) Aksu, Cinar Ward, David Bray-Ward, Patricia Demir, Ramazan Huszár Gábor
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6.

001-es BibID:BIBFORM040368
Első szerző:Delpiano, Elena
Cím:Frequency of chromosomal aneuploidis and diploidies in oligozoospermic and normozoospermic men / Delpiano, E., Jakab, A., Kovács, T., Ward, D. C., Revelli, A., Huszar, G.
Dátum:2003
ISSN:1472-6483
Tárgyszavak:Orvostudományok Klinikai orvostudományok idézhető absztrakt
Megjelenés:Reproductive Biomedicine Online. - 7 : Suppl. 1. (2003), p. 12-13. -
További szerzők:Jakab Attila (1964-) (szülész-nőgyógyász, endokrinológus) Kovács Tamás (1959-) (szülész-nőgyógyász, humángenetikus) Ward, David Revelli, Alberto Huszár Gábor
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7.

001-es BibID:BIBFORM038979
Első szerző:Huszár Gábor
Cím:A double chamber device for advanced sperm testing : semen analysis and an assay for sperm maturity by hyaluronic acid (HA) binding / G. Huszár, C. Celik-Ozenci, A. Jakab, L. Vigue
Dátum:2002
ISSN:0196-3635
Tárgyszavak:Orvostudományok Klinikai orvostudományok idézhető absztrakt
külföldön készült közlemény
Megjelenés:Journal of Andrology 23 : Suppl. (2002), p. 55. -
További szerzők:Celik-Ozenci, Ciler Jakab Attila (1964-) (szülész-nőgyógyász, endokrinológus) Vigue, Lynne
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8.

001-es BibID:BIBFORM023099
Első szerző:Huszár Gábor
Cím:Hyaluronic acid binding by human sperm : andrology evaluation of male fertility and sperm selection for intracytoplasmic sperm injection / Gabor Huszar, Attila Jakab, Ciler Celik-Ozenci, Leyla G. Sati
Dátum:2006
Tárgyszavak:Orvostudományok Klinikai orvostudományok könyvfejezet
andrology
sperm function
sperm selection
Megjelenés:Male Infertility - Diagnosis and Treatment / eds. Kruger T. F., Oehninger S. C.. - p. 413-425
További szerzők:Jakab Attila (1964-) (szülész-nőgyógyász, endokrinológus) Celik-Ozenci, Ciler Sati, Leyla G.
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9.

001-es BibID:BIBFORM023097
Első szerző:Huszár Gábor
Cím:Sperm testing by hyaluronic acid binding : andrologic laboratory assessment and sperm selection for ICSI / Gabor Huszar, Attila Jakab, Ciler Celik-Ozenci, Denny Sakkas, Tamas Kovacs, Lynne Vigue
Dátum:2003
Tárgyszavak:Orvostudományok Klinikai orvostudományok könyvfejezet
andrology
sperm function
sperm selection
Megjelenés:Biotechnology of Human Reproduction / eds. Revelli A., Tur-Kaspa I., Massabrio M. - p. 149-157
További szerzők:Jakab Attila (1964-) (szülész-nőgyógyász, endokrinológus) Celik-Ozenci, Ciler Sakkas, Denny Kovács Tamás Vigue, Lynne
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10.

001-es BibID:BIBFORM023079
Első szerző:Huszár Gábor
Cím:Hyaluronic acid binding ability of human sperm reflects cellular maturity and fertilizing potential : selection of sperm for intracytoplasmic sperm injection / Huszár G., Ozkavukcu S., Jakab A., Celik-Ozenci C., Sati L. G., Cayli S.
Dátum:2006
ISSN:1040-872X
Megjegyzések:The current concepts of sperm biochemical markers and the central role of the HspA2 chaperone protein, a measure of sperm cellular maturity and fertilizing potential, are reviewed. RECENT FINDINGS: Because HspA2 is a component of the synaptonemal complex, low HspA2 levels and increased frequency of chromosomal aneuploidies are related in diminished maturity sperm. We also suggest a relationship between HspA2 expression in elongating spermatids and events of late spermiogenesis, such as cytoplasmic extrusion and plasma membrane remodeling that aid the formation of the zona pellucida binding and hyaluronic acid binding sites. The presence of hyaluronic acid receptor on the plasma membrane of mature sperm, coupled with hyaluronic acid coated glass or plastic surfaces, facilitates testing of sperm function and selection of single mature sperm for intracytoplasmic sperm injection. The frequencies of sperm with chromosomal disomy are reduced approximately fourfold to fivefold in hyaluronic acid selected sperm compared with semen sperm, comparable to the increase in such abnormalities in intracytoplasmic sperm injection offspring. Hyaluronic acid binding also excludes immature sperm with cytoplasmic extrusion, persistent histones, and DNA chain breaks. SUMMARY: Hyaluronic acid mediated sperm selection is a novel technique that is comparable to sperm zona pellucida binding. Hyaluronic acid selected sperm will also alleviate the risks related to intracytoplasmic sperm injection fertilization with sperm of diminished maturity that currently cause worldwide concern.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
chromosomal disomy and diploidy
intracytoplasmic sperm injection
sperm maturation
zona pellucida binding
Megjelenés:Current Opinion In Obstetrics & Gynecology. - 18 : 3 (2006), p. 260-267. -
További szerzők:Ozkavukcu, Sinan Jakab Attila (1964-) (szülész-nőgyógyász, endokrinológus) Celik-Ozenci, Ciler Sati, Leyla G. Cayli, Sevil
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11.

001-es BibID:BIBFORM002256
Első szerző:Huszár Gábor
Cím:Fertility testing and ICSI sperm selection by hyaluronic acid binding : clinical and genetic aspects / Gabor Huszar, Attila Jakab, Denny Sakkas, Ciler-Celik Ozenci, Sevil Cayli, Elena Delpiano, Sinan Ozkavukcu
Dátum:2007
Megjegyzések:The testis-expressed chaperone protein, HspA2 (previously creatine kinase M isoform) was established as a measure of human sperm cellular maturity, function and fertility. The presence of HspA2 in the synaptonemal complex is likely to link low HspA2 expression and increased frequency of chromosomal aneuploidies in arrested-maturity spermatozoa. A relationship also exists between HspA2 expression in elongating spermatids and the associated spermatogenetic events, including plasma membrane remodelling and the formation of zona pellucida and hyaluronic acid (HA) binding sites. The HA receptor of mature spermatozoa, when coupled with HA-coated slides and/or Petri dishes, allows visual observation of sperm-HA binding, providing a basis for sperm maturity testing, a major improvement in semen evaluation, and selection of mature spermatozoa for intracytoplasmic sperm injection (ICSI). Thus, in HA-selected spermatozoa the frequency of chromosomal disomy and diploidy is reduced 4- to 6-fold compared with semen sperm fractions. This reduction is similar to the increase in numerical chromosomal aberrations in ICSI children. Combined studies of sperm shape and chromosome probes demonstrated that sperm morphology does not aid selection of haploid spermatozoa. The HA-mediated sperm selection is a novel and efficient technique that may alleviate potential problems related to ICSI fertilization with visually selected spermatozoa.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Reproductive Biomedicine Online 14 : 5 (2007), p. 650-663. -
További szerzők:Jakab Attila (1964-) (szülész-nőgyógyász, endokrinológus) Sakkas, Denny Ozenci, Ciler-Celik Cayli, Sevil Delpiano, Elena Ozkavukcu, Sinan
Internet cím:elektronikus változat
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12.

001-es BibID:BIBFORM046563
Első szerző:Jakab Attila (szülész-nőgyógyász, endokrinológus)
Cím:Efficacy of the swim-up method in eliminating sperm with diminished maturity and aneuploidy / Attila Jakab, Tamas Kovacs, Zoltan Zavaczki, Antal Borsos, Patricia Bray-Ward, David Ward, Gabor Huszar
Dátum:2003
ISSN:1460-2350
Megjegyzések:We have previously shown that after 80% Percoll centrifugation there is an overall 2.7-fold reduction of sperm with chromosomal disomies and diploidies (3.2-fold and 2.0-fold respectively), and of sperm with diminished maturity as detected by cytoplasmic retention. The relationship between disomies and immature sperm was r = 0.7, suggesting that disomy primarily originates in immature sperm. In the present work we studied the efficacy of the swim-up method in elimination of sperm with diminished maturity and with chromosomal aberrations in the swim-up sperm fractions of 10 patients (sperm concentration: 20 ? 3.9 x 106/ml, range 8.9-45.5; sperm motility: 45.2 ? 2.4, all mean ? SEM). METHODS: The validity of the study was enhanced by assessing each sperm fraction with three-colour (X, Y and 17; 5000 sperm) and two-colour (10 and 11; 5000 sperm) chromosome probes using fluorescence in-situ hybridization (FISH). Thus, in each sample 10 000 sperm were evaluated. The incidence of diminished maturity sperm was assessed with creatine kinase immunocytochemistry. RESULTS: In the swim-up fractions there was a reduction in the frequencies of disomic sperm, whether considering the sex chromosomes (1.4-fold) or the three autosomal chromosomes (1.5-fold based on the aggregate frequencies of disomy 10, 11 and 17). There was also a 1.5-fold reduction in diminished maturity sperm, indicating a relationship between the proportion of immature sperm and chromosomal aneuploidies (r = 0.46, P < 0.05, n = 20). Diploid sperm were reduced at a 2.7-fold rate, whether assessed with two- or three-colour FISH. There was a slight increase in the X/Y ratios. CONCLUSIONS: Swim-up reduces the proportion of sperm with chromosomal aberrations and of sperm with diminished maturity. When compared with the results of the previous study with gradient centrifugation performed on semen samples with similar quality, the efficacy after swim-up is lower for disomies and higher for diploidies than that of gradient centrifugation.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Human Reproduction. - 18 : 7 (2003), p. 1481-1488. -
További szerzők:Kovács Tamás (1959-) (szülész-nőgyógyász, humángenetikus) Zavaczki Zoltán Borsos Antal (1939-) (szülész-nőgyógyász, gyermeknőgyógyász) Bray-Ward, Patricia Ward, David Huszár Gábor
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