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001-es BibID:	BIBFORM023079
Első szerző:	Huszár Gábor
Cím:	Hyaluronic acid binding ability of human sperm reflects cellular maturity and fertilizing potential : selection of sperm for intracytoplasmic sperm injection / Huszár G., Ozkavukcu S., Jakab A., Celik-Ozenci C., Sati L. G., Cayli S.
Dátum:	2006
ISSN:	1040-872X
Megjegyzések:	The current concepts of sperm biochemical markers and the central role of the HspA2 chaperone protein, a measure of sperm cellular maturity and fertilizing potential, are reviewed. RECENT FINDINGS: Because HspA2 is a component of the synaptonemal complex, low HspA2 levels and increased frequency of chromosomal aneuploidies are related in diminished maturity sperm. We also suggest a relationship between HspA2 expression in elongating spermatids and events of late spermiogenesis, such as cytoplasmic extrusion and plasma membrane remodeling that aid the formation of the zona pellucida binding and hyaluronic acid binding sites. The presence of hyaluronic acid receptor on the plasma membrane of mature sperm, coupled with hyaluronic acid coated glass or plastic surfaces, facilitates testing of sperm function and selection of single mature sperm for intracytoplasmic sperm injection. The frequencies of sperm with chromosomal disomy are reduced approximately fourfold to fivefold in hyaluronic acid selected sperm compared with semen sperm, comparable to the increase in such abnormalities in intracytoplasmic sperm injection offspring. Hyaluronic acid binding also excludes immature sperm with cytoplasmic extrusion, persistent histones, and DNA chain breaks. SUMMARY: Hyaluronic acid mediated sperm selection is a novel technique that is comparable to sperm zona pellucida binding. Hyaluronic acid selected sperm will also alleviate the risks related to intracytoplasmic sperm injection fertilization with sperm of diminished maturity that currently cause worldwide concern.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban chromosomal disomy and diploidy intracytoplasmic sperm injection sperm maturation zona pellucida binding
Megjelenés:	Current Opinion In Obstetrics & Gynecology. - 18 : 3 (2006), p. 260-267. -
További szerzők:	Ozkavukcu, Sinan Jakab Attila (1964-) (szülész-nőgyógyász, endokrinológus) Celik-Ozenci, Ciler Sati, Leyla G. Cayli, Sevil
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat DOI
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001-es BibID:	BIBFORM002256
Első szerző:	Huszár Gábor
Cím:	Fertility testing and ICSI sperm selection by hyaluronic acid binding : clinical and genetic aspects / Gabor Huszar, Attila Jakab, Denny Sakkas, Ciler-Celik Ozenci, Sevil Cayli, Elena Delpiano, Sinan Ozkavukcu
Dátum:	2007
Megjegyzések:	The testis-expressed chaperone protein, HspA2 (previously creatine kinase M isoform) was established as a measure of human sperm cellular maturity, function and fertility. The presence of HspA2 in the synaptonemal complex is likely to link low HspA2 expression and increased frequency of chromosomal aneuploidies in arrested-maturity spermatozoa. A relationship also exists between HspA2 expression in elongating spermatids and the associated spermatogenetic events, including plasma membrane remodelling and the formation of zona pellucida and hyaluronic acid (HA) binding sites. The HA receptor of mature spermatozoa, when coupled with HA-coated slides and/or Petri dishes, allows visual observation of sperm-HA binding, providing a basis for sperm maturity testing, a major improvement in semen evaluation, and selection of mature spermatozoa for intracytoplasmic sperm injection (ICSI). Thus, in HA-selected spermatozoa the frequency of chromosomal disomy and diploidy is reduced 4- to 6-fold compared with semen sperm fractions. This reduction is similar to the increase in numerical chromosomal aberrations in ICSI children. Combined studies of sperm shape and chromosome probes demonstrated that sperm morphology does not aid selection of haploid spermatozoa. The HA-mediated sperm selection is a novel and efficient technique that may alleviate potential problems related to ICSI fertilization with visually selected spermatozoa.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	Reproductive Biomedicine Online 14 : 5 (2007), p. 650-663. -
További szerzők:	Jakab Attila (1964-) (szülész-nőgyógyász, endokrinológus) Sakkas, Denny Ozenci, Ciler-Celik Cayli, Sevil Delpiano, Elena Ozkavukcu, Sinan
Internet cím:	elektronikus változat
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