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001-es BibID:BIBFORM023074
Első szerző:Celik-Ozenci, Ciler
Cím:Sperm selection for ICSI : shape properties do not predict the absence or presence of numerical chromosomal aberrations / Ciler Celik-Ozenci, Attila Jakab, Tamas Kovacs, Jillian Catalanotti, Ramazan Demir, Patricia Bray-Ward, David Ward, Gabor Huszar
Dátum:2004
ISSN:0268-1161
Megjegyzések:We hypothesize that the potential relationship between abnormal sperm morphology and increased frequency of numerical chromosomal aberrations is based on two attributes of diminished sperm maturity: (i) cytoplasmic retention and consequential sperm shape abnormalities; and (ii) meiotic errors caused by low levels of the HspA2 chaperone, a component of the synaptonemal complex. Because sperm morphology and aneuploidies were assessed in semen, but not in the same spermatozoa, previous studies addressing this relationship were inconclusive. We recently demonstrated that sperm shape is preserved following fluorescence in situ hybridization (FISH). Thus, we examined the shape and chromosomal aberrations in the same sperm. METHODS: We performed phase contrast microscopy and FISH, using centromeric probes for chromosomes X, Y, 10, 11 and 17 in 15 men. The fluorescence and respective phase contrast images were digitized using the Metamorph program. We studied 1286 sperm (256 disomic, 130 diploid and 900 haploid sperm) by three criteria: head and tail dimensions, head shape and Kruger strict morphology. Furthermore, in each analysis, we considered whether disomic or diploid sperm may be distinguished from haploid sperm. RESULTS: There was an overall, but not discriminative, relationship between abnormal sperm dimensions or shape and increased frequencies of numerical chromosomal aberrations. However, approximately 68 of the 256 disomic, and four of 130 diploid sperm showed head and tail dimensions comparable with the most normal, lowest tertile of the 900 haploid spermatozoa. Considering all 1286 sperm, among those with the most regular, symmetrical shape (n = 367), there were 63 and five with disomic and diploid nuclei, respectively. In line with these findings, among the 256 disomic sperm, 10% were Kruger normal. CONCLUSIONS: Sperm dimensions or shape are not reliable attributes in selection of haploid sperm for ICSI.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
spermium aneuploidia
Megjelenés:Human Reproduction. - 19 : 9 (2004), p. 2052-2059. -
További szerzők:Jakab Attila (1964-) (szülész-nőgyógyász, endokrinológus) Kovács Tamás (1959-) (szülész-nőgyógyász, humángenetikus) Catalanotti, Jillian Demir, Ramazan Bray-Ward, Patricia Ward, David Huszár Gábor
Internet cím:DOI
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2.

001-es BibID:BIBFORM023072
Első szerző:Celik-Ozenci, Ciler
Cím:Human Sperm Maintain Their Shape Following Decondensation and Denaturation for Fluorescent In Situ Hybridization : shape Analysis and Objective Morphometry / Celik-Ozenci C., Catalanotti J., Jakab A., Aksu C., Ward D., Bray-Ward P., Demir R., Huszar G.
Dátum:2003
ISSN:0006-3363
Megjegyzések:The relationship between abnormal sperm morphology and chromosomal aberrations has been of interest. Thus far, however, studies have focused on frequencies of sperm with either abnormal morphology or aneuploidies in semen samples, not on detection of individual spermatozoa exhibiting both abnormal morphology and aneuploidy. To assess the feasibility of simultaneous evaluation of both attributes in an individual sperm cell, we investigated whether sperm shape is preserved after decondensation and denaturation, procedures that are required for fluorescent in situ hybridization (FISH). On 21 slides, 395 sperm were fixed, photographed, and then digitized by the computer-assisted Metamorph morphometry program for individual evaluation before decondensation. To establish whether sperm of various shapes would behave in similar manners, the cells were also classified, according to their head shapes, into symmetrical (n = 115), asymmetrical (n = 115), irregular (n = 115), and amorphous (n = 50) categories. Following decondensation and subsequent denaturation, sperm that had been photographed initially were relocalized and digitized for morphometry. Head area, perimeter, long axis, short axis, shape factor, and tail length were evaluated in each of the 395 sperm in both the native and decondensed states. After the decondensation and denaturation protocol of the FISH procedure, the sperm exhibited a proportional increase in dimensions as compared to their original sizes. Their initial shapes were preserved with high fidelity whether the sperm were in the symmetrical, asymmetrical, irregular, or amorphous categories. Hybridization with the chromosome probes had no further effect on sperm shape or size. We provide images to demonstrate how these findings facilitate studies about the relationship between sperm shape and chromosomal content or aberrations in individual spermatozoa.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
sperm
Megjelenés:Biology of Reproduction. - 69 : 4 (2003), p. 1347-1355. -
További szerzők:Catalanotti, Jillian Jakab Attila (1964-) (szülész-nőgyógyász, endokrinológus) Aksu, Cinar Ward, David Bray-Ward, Patricia Demir, Ramazan Huszár Gábor
Internet cím:DOI
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3.

001-es BibID:BIBFORM046563
Első szerző:Jakab Attila (szülész-nőgyógyász, endokrinológus)
Cím:Efficacy of the swim-up method in eliminating sperm with diminished maturity and aneuploidy / Attila Jakab, Tamas Kovacs, Zoltan Zavaczki, Antal Borsos, Patricia Bray-Ward, David Ward, Gabor Huszar
Dátum:2003
ISSN:1460-2350
Megjegyzések:We have previously shown that after 80% Percoll centrifugation there is an overall 2.7-fold reduction of sperm with chromosomal disomies and diploidies (3.2-fold and 2.0-fold respectively), and of sperm with diminished maturity as detected by cytoplasmic retention. The relationship between disomies and immature sperm was r = 0.7, suggesting that disomy primarily originates in immature sperm. In the present work we studied the efficacy of the swim-up method in elimination of sperm with diminished maturity and with chromosomal aberrations in the swim-up sperm fractions of 10 patients (sperm concentration: 20 ? 3.9 x 106/ml, range 8.9-45.5; sperm motility: 45.2 ? 2.4, all mean ? SEM). METHODS: The validity of the study was enhanced by assessing each sperm fraction with three-colour (X, Y and 17; 5000 sperm) and two-colour (10 and 11; 5000 sperm) chromosome probes using fluorescence in-situ hybridization (FISH). Thus, in each sample 10 000 sperm were evaluated. The incidence of diminished maturity sperm was assessed with creatine kinase immunocytochemistry. RESULTS: In the swim-up fractions there was a reduction in the frequencies of disomic sperm, whether considering the sex chromosomes (1.4-fold) or the three autosomal chromosomes (1.5-fold based on the aggregate frequencies of disomy 10, 11 and 17). There was also a 1.5-fold reduction in diminished maturity sperm, indicating a relationship between the proportion of immature sperm and chromosomal aneuploidies (r = 0.46, P < 0.05, n = 20). Diploid sperm were reduced at a 2.7-fold rate, whether assessed with two- or three-colour FISH. There was a slight increase in the X/Y ratios. CONCLUSIONS: Swim-up reduces the proportion of sperm with chromosomal aberrations and of sperm with diminished maturity. When compared with the results of the previous study with gradient centrifugation performed on semen samples with similar quality, the efficacy after swim-up is lower for disomies and higher for diploidies than that of gradient centrifugation.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Human Reproduction. - 18 : 7 (2003), p. 1481-1488. -
További szerzők:Kovács Tamás (1959-) (szülész-nőgyógyász, humángenetikus) Zavaczki Zoltán Borsos Antal (1939-) (szülész-nőgyógyász, gyermeknőgyógyász) Bray-Ward, Patricia Ward, David Huszár Gábor
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DOI
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4.

001-es BibID:BIBFORM038992
Első szerző:Jakab Attila (szülész-nőgyógyász, endokrinológus)
Cím:Association between diploidy and double heads in spermatozoa arising from meiosis I and meiosis II / Jakab A., Kovacs T., Borsos A., Ward D. C., Bray-Ward P., Huszar G.
Dátum:2003
ISSN:0268-1161
Tárgyszavak:Orvostudományok Klinikai orvostudományok idézhető absztrakt
egyetemen (Magyarországon) készült közlemény
Megjelenés:Human Reproduction. - 18 : Suppl. 1. (2003), p. 188. -
További szerzők:Kovács Tamás (1959-) (szülész-nőgyógyász, humángenetikus) Borsos Antal (1939-) (szülész-nőgyógyász, gyermeknőgyógyász) Ward, David Bray-Ward, Patricia Huszár Gábor
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5.

001-es BibID:BIBFORM038985
Első szerző:Jakab Attila (szülész-nőgyógyász, endokrinológus)
Cím:Selection of sperm with low aneuploidy frequency for ICSI / Jakab A., Sakkas D., Celik-Ozenci C., Vigue L., Ward D., Bray-Ward P., Huszar G.
Dátum:2002
ISSN:0268-1161
Tárgyszavak:Orvostudományok Klinikai orvostudományok idézhető absztrakt
külföldön készült közlemény
Megjelenés:Human Reproduction. - 17 : Suppl. 1. (2002), p. 35-36. -
További szerzők:Sakkas, Denny Celik-Ozenci, Ciler Vigue, Lynne Ward, D. Bray-Ward, Patricia Huszár Gábor
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