Találati lista | Tudóstér

001-es BibID:	BIBFORM038980
Első szerző:	Celik-Ozenci, Ciler
Cím:	Mature and fertile sperm selectively bind to hyaluronic acid : cytoplasmic content, HspA2 levels, chromatin maturity, shape and ICSI sperm selection / Celik-Ozenci C., Jakab A., Vigue L., Demir R., Huszar G.
Dátum:	2002
ISSN:	1071-5576
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idézhető absztrakt külföldön készült közlemény
Megjelenés:	Journal of The Society for Gynecologic Investigation 9 (2002), p. 849. -
További szerzők:	Jakab Attila (1964-) (szülész-nőgyógyász, endokrinológus) Vigue, Lynne Demir, Ramazan Huszár Gábor
Borító:
Saját polcon:

001-es BibID:	BIBFORM038987
Első szerző:	Celik-Ozenci, Ciler
Cím:	Can one visually select aneuploidy-free sperm : a study of morphometry and fluorescence in-situ hybridization / Celik-Ozenci C. Catalanotti J., Jakab A. Kovacs T. Demir R. Huszar G.
Dátum:	2002
ISSN:	0268-1161
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idézhető absztrakt külföldön készült közlemény
Megjelenés:	Human Reproduction 17 : Suppl. 1. (2002), p. 41. -
További szerzők:	Catalanotti, Jillian Jakab Attila (1964-) (szülész-nőgyógyász, endokrinológus) Kovács Tamás (1959-) (szülész-nőgyógyász, humángenetikus) Demir, Ramazan Huszár Gábor
Internet cím:	Szerző által megadott URL
Borító:
Saját polcon:

001-es BibID:	BIBFORM023074
Első szerző:	Celik-Ozenci, Ciler
Cím:	Sperm selection for ICSI : shape properties do not predict the absence or presence of numerical chromosomal aberrations / Ciler Celik-Ozenci, Attila Jakab, Tamas Kovacs, Jillian Catalanotti, Ramazan Demir, Patricia Bray-Ward, David Ward, Gabor Huszar
Dátum:	2004
ISSN:	0268-1161
Megjegyzések:	We hypothesize that the potential relationship between abnormal sperm morphology and increased frequency of numerical chromosomal aberrations is based on two attributes of diminished sperm maturity: (i) cytoplasmic retention and consequential sperm shape abnormalities; and (ii) meiotic errors caused by low levels of the HspA2 chaperone, a component of the synaptonemal complex. Because sperm morphology and aneuploidies were assessed in semen, but not in the same spermatozoa, previous studies addressing this relationship were inconclusive. We recently demonstrated that sperm shape is preserved following fluorescence in situ hybridization (FISH). Thus, we examined the shape and chromosomal aberrations in the same sperm. METHODS: We performed phase contrast microscopy and FISH, using centromeric probes for chromosomes X, Y, 10, 11 and 17 in 15 men. The fluorescence and respective phase contrast images were digitized using the Metamorph program. We studied 1286 sperm (256 disomic, 130 diploid and 900 haploid sperm) by three criteria: head and tail dimensions, head shape and Kruger strict morphology. Furthermore, in each analysis, we considered whether disomic or diploid sperm may be distinguished from haploid sperm. RESULTS: There was an overall, but not discriminative, relationship between abnormal sperm dimensions or shape and increased frequencies of numerical chromosomal aberrations. However, approximately 68 of the 256 disomic, and four of 130 diploid sperm showed head and tail dimensions comparable with the most normal, lowest tertile of the 900 haploid spermatozoa. Considering all 1286 sperm, among those with the most regular, symmetrical shape (n = 367), there were 63 and five with disomic and diploid nuclei, respectively. In line with these findings, among the 256 disomic sperm, 10% were Kruger normal. CONCLUSIONS: Sperm dimensions or shape are not reliable attributes in selection of haploid sperm for ICSI.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban spermium aneuploidia
Megjelenés:	Human Reproduction. - 19 : 9 (2004), p. 2052-2059. -
További szerzők:	Jakab Attila (1964-) (szülész-nőgyógyász, endokrinológus) Kovács Tamás (1959-) (szülész-nőgyógyász, humángenetikus) Catalanotti, Jillian Demir, Ramazan Bray-Ward, Patricia Ward, David Huszár Gábor
Internet cím:	DOI Intézményi repozitóriumban (DEA) tárolt változat
Borító:
Saját polcon:

001-es BibID:	BIBFORM023072
Első szerző:	Celik-Ozenci, Ciler
Cím:	Human Sperm Maintain Their Shape Following Decondensation and Denaturation for Fluorescent In Situ Hybridization : shape Analysis and Objective Morphometry / Celik-Ozenci C., Catalanotti J., Jakab A., Aksu C., Ward D., Bray-Ward P., Demir R., Huszar G.
Dátum:	2003
ISSN:	0006-3363
Megjegyzések:	The relationship between abnormal sperm morphology and chromosomal aberrations has been of interest. Thus far, however, studies have focused on frequencies of sperm with either abnormal morphology or aneuploidies in semen samples, not on detection of individual spermatozoa exhibiting both abnormal morphology and aneuploidy. To assess the feasibility of simultaneous evaluation of both attributes in an individual sperm cell, we investigated whether sperm shape is preserved after decondensation and denaturation, procedures that are required for fluorescent in situ hybridization (FISH). On 21 slides, 395 sperm were fixed, photographed, and then digitized by the computer-assisted Metamorph morphometry program for individual evaluation before decondensation. To establish whether sperm of various shapes would behave in similar manners, the cells were also classified, according to their head shapes, into symmetrical (n = 115), asymmetrical (n = 115), irregular (n = 115), and amorphous (n = 50) categories. Following decondensation and subsequent denaturation, sperm that had been photographed initially were relocalized and digitized for morphometry. Head area, perimeter, long axis, short axis, shape factor, and tail length were evaluated in each of the 395 sperm in both the native and decondensed states. After the decondensation and denaturation protocol of the FISH procedure, the sperm exhibited a proportional increase in dimensions as compared to their original sizes. Their initial shapes were preserved with high fidelity whether the sperm were in the symmetrical, asymmetrical, irregular, or amorphous categories. Hybridization with the chromosome probes had no further effect on sperm shape or size. We provide images to demonstrate how these findings facilitate studies about the relationship between sperm shape and chromosomal content or aberrations in individual spermatozoa.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban sperm
Megjelenés:	Biology of Reproduction. - 69 : 4 (2003), p. 1347-1355. -
További szerzők:	Catalanotti, Jillian Jakab Attila (1964-) (szülész-nőgyógyász, endokrinológus) Aksu, Cinar Ward, David Bray-Ward, Patricia Demir, Ramazan Huszár Gábor
Internet cím:	DOI Intézményi repozitóriumban (DEA) tárolt változat
Borító:
Saját polcon: