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001-es BibID:BIBFORM046563
Első szerző:Jakab Attila (szülész-nőgyógyász, endokrinológus)
Cím:Efficacy of the swim-up method in eliminating sperm with diminished maturity and aneuploidy / Attila Jakab, Tamas Kovacs, Zoltan Zavaczki, Antal Borsos, Patricia Bray-Ward, David Ward, Gabor Huszar
Dátum:2003
ISSN:1460-2350
Megjegyzések:We have previously shown that after 80% Percoll centrifugation there is an overall 2.7-fold reduction of sperm with chromosomal disomies and diploidies (3.2-fold and 2.0-fold respectively), and of sperm with diminished maturity as detected by cytoplasmic retention. The relationship between disomies and immature sperm was r = 0.7, suggesting that disomy primarily originates in immature sperm. In the present work we studied the efficacy of the swim-up method in elimination of sperm with diminished maturity and with chromosomal aberrations in the swim-up sperm fractions of 10 patients (sperm concentration: 20 ? 3.9 x 106/ml, range 8.9-45.5; sperm motility: 45.2 ? 2.4, all mean ? SEM). METHODS: The validity of the study was enhanced by assessing each sperm fraction with three-colour (X, Y and 17; 5000 sperm) and two-colour (10 and 11; 5000 sperm) chromosome probes using fluorescence in-situ hybridization (FISH). Thus, in each sample 10 000 sperm were evaluated. The incidence of diminished maturity sperm was assessed with creatine kinase immunocytochemistry. RESULTS: In the swim-up fractions there was a reduction in the frequencies of disomic sperm, whether considering the sex chromosomes (1.4-fold) or the three autosomal chromosomes (1.5-fold based on the aggregate frequencies of disomy 10, 11 and 17). There was also a 1.5-fold reduction in diminished maturity sperm, indicating a relationship between the proportion of immature sperm and chromosomal aneuploidies (r = 0.46, P < 0.05, n = 20). Diploid sperm were reduced at a 2.7-fold rate, whether assessed with two- or three-colour FISH. There was a slight increase in the X/Y ratios. CONCLUSIONS: Swim-up reduces the proportion of sperm with chromosomal aberrations and of sperm with diminished maturity. When compared with the results of the previous study with gradient centrifugation performed on semen samples with similar quality, the efficacy after swim-up is lower for disomies and higher for diploidies than that of gradient centrifugation.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Human Reproduction. - 18 : 7 (2003), p. 1481-1488. -
További szerzők:Kovács Tamás (1959-) (szülész-nőgyógyász, humángenetikus) Zavaczki Zoltán Borsos Antal (1939-) (szülész-nőgyógyász, gyermeknőgyógyász) Bray-Ward, Patricia Ward, David Huszár Gábor
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DOI
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2.

001-es BibID:BIBFORM023098
Első szerző:Kovács Tamás
Cím:Preparation of sperm fractions and individual sperm with low levels of chromosomal aneuploidies for assisted reproduction / Tamas Kovacs, Attila Jakab, Ertug Kovanci, Zoltan Zavaczki, Denny Sakkas, Gabor Huszar
Dátum:2003
Tárgyszavak:Orvostudományok Klinikai orvostudományok könyvfejezet
andrology
sperm function
sperm selection
Megjelenés:Essential IVF / eds. Van Blerkom J., Gregory L. - p. 202-213
További szerzők:Jakab Attila (1964-) (szülész-nőgyógyász, endokrinológus) Kovanci, Ertug Zavaczki Zoltán Sakkas, Denny Huszár Gábor
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3.

001-es BibID:BIBFORM023078
Első szerző:Zavaczki Zoltán
Cím:Dimensional assessment of X-bearing and Y-bearing haploid and disomic human sperm with the use of fluorescence in situ hybridization and objective morphometry / Zavaczki Z., Celik-Ozenci C., Ovari L., Jakab A., Sati G. L., Ward D. C., Huszar G.
Dátum:2006
ISSN:0015-0282
Megjegyzések:To explore the dimensional attributes of haploid and disomic X-bearing and Y-bearing spermatozoa. Design: Morphometric evaluation of more than 2,000 X-bearing and Y-bearing spermatozoa after identification of the genotype with fluorescence in situ hybridization. Setting: Academic clinical and research andrology laboratory. Main Outcome Measure(s): Sperm head area, perimeter, long axis, short axis, shape factor, elliptical form factor (long axis/short axis), and tail length. Result(s): We found no differences in dimensions or dimensional distributions between X-bearing and Y-bearing spermatozoa, whether in the native or the decondensed state, or in oligozoospermic or normozoospermic men. There were inconsistent differences and a 70% overlap in the dimensions of haploid and disomic spermatozoa. The other 30% of sperm with disomic nuclei were either smaller or larger compared to haploid spermatozoa. Conclusion(s): There are no differences, or distinguishing characteristics, in dimensions or dimensional distri- butions between X-bearing and Y-bearing spermatozoa. Dimensional attributes do not discriminate between dysomic and haploid spermatozoa.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Sperm
X-bearing spermatozoa
dimensional attributes
haploid
dosomic
Megjelenés:Fertility And Sterility. - 85 : 1 (2006), p. 121-127. -
További szerzők:Celik-Ozenci, Ciler Óvári László (szülész-nőgyógyász, sebész) Jakab Attila (1964-) (szülész-nőgyógyász, endokrinológus) Sati, Leyla G. Ward, David Huszár Gábor
Internet cím:DOI
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