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001-es BibID:	BIBFORM101152
Első szerző:	Ni, Dong-Qi
Cím:	Titanium dioxide nanoparticles perturb the blood-testis barrier via disruption of actin-based cell adhesive function / Dong-Qi Ni, Dan-Dan Ma, Shuang-Li Hao, Wan-Xi Yang, Tamas Kovacs, Fu-Qing Tan
Dátum:	2021
ISSN:	1945-4589
Megjegyzések:	As one of the most commonly used nanoparticles, titanium dioxide nanoparticles (TiO2-NPs) are widely used as coating reagents in cosmetics, medicine and other industries. The increasing risk of exposure to TiO2-NPs raises concerns about their safety. In this study, we investigated the mechanism by which TiO2-NPs cross the bloodtestis barrier (BTB). TM-4 cells were selected as an in vitro Sertoli cell model of BTB. Cell viability, cell morphological changes, apoptosis, oxidative damage, and the expression levels of actin regulatory and tight junction (TJ) proteins were assessed in TM-4 cells treated with 3-nm and 24-nm TiO2-NPs. Cells treated with 3- nm TiO2-NPs exhibited increased cytotoxicity and decreased Annexin II expression, whereas cells treated with 24-nm TiO2-NPs exhibited increased Arp 3 and c-Src expression. Both TiO2-NPs induced significant oxidative stress, decreased the expression of TJ proteins (occludin, ZO-1 and claudin 5), damaged the TJ structure, and exhibited enlarged gaps between TM-4 cells. Our results indicated that both TiO2-NPs crossed the BTB by disrupting actin-based adhesive junctions of TM-4 cells; however, apoptosis was not observed. Our results provide new insights into how TiO2-NPs cross the BTB.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk TiO2-NPs blood-testis barrier TM-4 cell tight junction actin
Megjelenés:	Aging. - 13 : 23 (2021), p. 25440-25452. -
További szerzők:	Ma, Dan-Dan Hao, Shuang-Li Yang, Wan-Xi Kovács Tamás (1959-) (szülész-nőgyógyász, humángenetikus) Tan, Fu-Qing
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM015677
Első szerző:	Sun, Xiao
Cím:	The role of actin and myosin during spermatogenesis / Sun Xiao, Kovacs Tamas, Hu Yan-Jun, Yang Wan-Xi
Dátum:	2010
ISSN:	0301-4851
Megjegyzések:	Spermatogenesis is a transitionary process in which the diploid spermatogonia transform into haploid mature spermatozoa. Actin and myosin have been implicated in various aspects during spermatogenesis. Actin is present in the form of monomer, oligomer and polymer within cells, the latter is called microfilament. There are five actin-containing structures during spermatogenesis, i.e., ectoplasmic specialization, acroplaxome, manchette in mammals, actin cones in Drosophila and acroframosome in Caridean shrimp. They are involved in the shaping and differentiating of spermatids. Along with spermatogenesis, the actin cytoskeletons show active remodeling in this process. Some actin binding or actin regulated proteins have been demonstrated to regulate dynamic changes of the actin-containing structures. Myosin, actin-dependent molecular motor, plays an important role during spermatogenesis, such as involving in acrosome biogenesis, vesicle transport, gene transcription and nuclear shaping. The actin cytoskeleton and actin binding/regulated proteins cooperate to facilitate spermatogenesis. In this review, we summarize the existing knowledge about the cytoskeletal structures consisting of actin, actin binding/regulated proteins and myosin during spermatogenesis.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban actin myosin spermatogenesis acrosome biogenesis nuclear shaping
Megjelenés:	Molecular Biology Reports. - 38 : 6 (2010), p. 3993-4001. -
További szerzők:	Kovács Tamás (1959-) (szülész-nőgyógyász, humángenetikus) Hu, Yan-Jun Yang, Wan-Xi
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM083491
035-os BibID:	(PMID)31446094
Első szerző:	Wei, Ya-Lan
Cím:	C-terminal kinesin motor es-KIFC1 regulates nuclear formation during spermiogenesis in Chinese mitten crab Eriocheir sinensis / Ya-Lan Wei, Tong Yang, Tamas Kovacs, Wan-Xi Yang
Dátum:	2019
ISSN:	0378-1119
Megjegyzések:	Kinesin-14 motor es-kifc1 is highly expressed in the male reproductive system of the Chinese mitten crab Eriocheir sinensis (E. sinensis). In addition to acrosomal formation, es-KIFC1 also tightly surrounds the nucleus and its specific mechanism remains unknown. During spermatogenesis, sperm nucleus dents into a cup-shaped structure with several radial arms and completed the nuclear decondensation. In this study, the spatial expression pattern of es-KIFC1 indicates a potential function in nuclear formation with the nuclear localization sequence (NLS) on N-terminal domain which is crucial for the translocation of es-KIFC1 into the nucleus. The Motor domain is associated with microtubule modulation and the Golgi vesicles positioning. Furthermore, the expression level of es-KIFC1 is not only related to the seasonal variation of crustacean development, but also associates with mature sperm storage. The double strand RNA (dsRNA) mediated RNA interference manifests that the cup-shaped sperm nucleus is remarkably malformed and even separates the chromatin throughout the nuclei at the last stage of spermiogenesis. Besides, the sperm nucleus almost disperses its structure and separates the chromatin into several segments throughout the nucleus showing an asymmetrical performance without cytoskeleton. In summary, these results indicate the importance of es-KIFC1 in microtubule positioning and the maintenance of the mature sperm nuclei.
Tárgyszavak:	Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk Eriocheir sinensis Es-KIFC1 Nucleus RNAi Spermatogenesis
Megjelenés:	Gene. - 719 (2019), p. 1-10. -
További szerzők:	Yang, Tong Kovács Tamás (1959-) (szülész-nőgyógyász, humángenetikus) Yang, Wan-Xi
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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