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001-es BibID:BIBFORM047051
035-os BibID:PMID:2157500
Első szerző:Csortos Csilla (biokémikus)
Cím:Phosphorylase phosphatase activities of rat liver in streptozotocin-diabetes / Csilla Csortos, Ilona Farkas, Lloyd Sparks, Tamás Bányász, Tibor Kovács, Pál Gergely
Dátum:1990
ISSN:0167-4889
Megjegyzések:Protein phosphatase-1 and 2A, accounting for all the hepatic activity regulating phosphorylase, were assayed in streptozotocin-induced (8 weeks) diabetic Wistar rats. Cytosolic protein phosphatase-1 and 2A were distinguished by chromatography on heparin-Sepharose and by inhibition with inhibitor-2. Approx. 25-35% increases in type-1 phosphorylase phosphatase activity measured in cytosols were registered in diabetic rats when compared with control and 24 h fasting animals. The enrichment of protein phosphatase-1 in the cytosol of streptozotocin-treated rat livers could not be attributed to the reduced glycogen content with the onset of diabetes, since this elevated level of type-1 phosphatase was not observed in fasting rats with low glycogen content. The translocation of type-1 phosphatase from the particulate fraction into the cytosol was also recorded in trypsin-treated samples of diabetic rat livers. The apparent molecular weight of type-1 phosphatase in the cytosol of control and fasted rats was 160,000 as judged by gel filtration. The type-1 phosphatase activity that was released from the particulate fraction by streptozotocin-induced diabetes identified a further enzyme species (Mr 110,000) in the cytosol. Our data imply that the higher levels of cytosolic protein phosphatase-1 in diabetic rat liver could be a consequence of the dissociation of the catalytic subunit of protein phosphatase-1 and the glycogen-binding subunit in rat livers.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Protein phosphatase
Hepatic metabolism
Diabetes
Fasting
Streptozotocin
Rat liver
Megjelenés:Biochimica et Biophysica Acta (BBA). Molecular Cell Research. - 1052 : 1 (1990), p. 235-241. -
További szerzők:Farkas Ilona (1953-) (biokémikus) Sparks, Lloyd Bányász Tamás (1960-) (élettanász) Kovács Tibor (1929-1994) (élettanász) Gergely Pál (1947-) (biokémikus)
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
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