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001-es BibID:BIBFORM076044
035-os BibID:(WoS)000436909600011 (Scopus)85054071643 (PubMed)29769426
Első szerző:Horváth Balázs (élettanász)
Cím:Effect of the intracellular calcium concentration chelator BAPTA acetoxy-methylester on action potential duration in canine ventricular myocytes / B. Horvath, N. Szentandrassy, R. Veress, D. Baranyai, K. Kistamas, J. Almassy, A. Toth, J. Magyar, T. Banyasz, P. P. Nanasi
Dátum:2018
Megjegyzések:Intracellular calcium concentration ([Ca2+]i) is often buffered by using the cell-permeant acetoxy-methylester form of the Ca2+ chelator BAPTA (BAPTA-AM) under experimental conditions. This study was designed to investigate the time-dependent actions of extracellularly applied BAPTA-AM on action potential duration (APD) in cardiac cells. Action potentials were recorded from enzymatically isolated canine ventricular myocytes with conventional sharp microelectrodes. The effect of BAPTA-AM on the rapid delayed rectifier K+ current (IKr) was studied using conventional voltage clamp and action potential voltage clamp techniques. APD was lengthened by 5 ?M BAPTA-AM - but not by BAPTA - and shortened by the Ca2+ ionophore A23187 in a time-dependent manner. The APD-lengthening effect of BAPTA-AM was strongly suppressed in the presence of nisoldipine, and enhanced in the presence of BAY K8644, suggesting that a shift in the [Ca2+]i-dependent inactivation of L-type Ca2+ current may be an important underlying mechanism. However, in the presence of the IKr-blocker dofetilide or E-4031 APD was shortened rather than lengthened by BAPTA-AM. Similarly, the APD-lengthening effect of 100 nM dofetilide was halved by the pretreatment with BAPTA-AM. In line with these results, IKr was significantly reduced by extracellularly applied BAPTA-AM under both conventional voltage clamp and action potential voltage clamp conditions. This inhibition of IKr was partially reversible and was not related to the Ca2+ chelator effect BAPTA-AM. The possible mechanisms involved in the APD-modifying effects of BAPTA-AM are discussed. It is concluded that BAPTA-AM has to be applied carefully to control [Ca2+]i in whole cell systems because of its direct inhibitory action on IKr.
Tárgyszavak:idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
calcium chelators
intracellular calcium concentration
action potential duration
cardiac ion currents
potassium ion
currents
ventricular myocytes
Megjelenés:Journal of Physiology and Pharmacology. - 69 : 1 (2018), p. 99-107. -
További szerzők:Szentandrássy Norbert (1976-) (élettanász) Veress Roland (1992-) (molekuláris biológus) Baranyai Dóra Kistamás Kornél (1986-) (biológus) Almássy János (1981-) (élettanász, biológus, angol-magyar szakfordító) Tóth A. (farmakológus) Magyar János (1961-) (élettanász) Bányász Tamás (1960-) (élettanász) Nánási Péter Pál (1956-) (élettanász)
Pályázati támogatás:NKFIHK-115397
NKFIH
NKFIH-K109736
NKFIH
NKFIH-PD120794
NKFIH
OTKA ANN-113273
OTKA
GINOP-2.3.2-15-2016-00040
GINOP
EFOP-3.6.2-16-2017-00006
EFOP
UNKP-17-4-III-DE-201
UNKP
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
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