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001-es BibID:BIBFORM060025
Első szerző:Kőszeghy Áron (Ph.D hallgató, élettanász)
Cím:Endocannabinoid signaling modulates neurons of the pedunculopontine nucleus (PPN) via astrocytes. / Áron Kőszeghy, Adrienn Kovács, Tamás Bíró, Péter Szűcs, János Vincze, Zoltán Hegyi, Miklós Antal, Balázs Pál
Dátum:2015
Megjegyzések:The pedunculopontine nucleus (PPN) is known as the cholinergic part of the reticular activating system (RAS) and it plays an important role in transitions of slow-wave sleep to REM sleep and wakefulness. Although both exogenous and endocannabinoids affect sleep, the mechanism of endocannabinoid neuromodulation has not been characterized at cellular level in the PPN. In this paper, we demonstrate that both neurons and glial cells from the PPN respond to cannabinoid type 1 (CB1) receptor agonists. The neuronal response can be depolarization or hyperpolarization, while astrocytes exhibit more frequent calcium waves. All these effects are absent in CB1 gene-deficient mice. Blockade of the fast synaptic neurotransmission or neuronal action potential firing does not change the effect on the neuronal membrane potential significantly, while inhibition of astrocytic calcium waves by thapsigargin diminishes the response. Inhibition of group I metabotropic glutamate receptors (mGluRs) abolishes hyperpolarization, whereas blockade of group II mGluRs prevents depolarization. Initially active neurons and glial cells display weaker responses partially due to the increased endocannabinoid tone in their environment. Taken together, we propose that cannabinoid receptor stimulation modulates PPN neuronal activity in the following manner: active neurons may elicit calcium waves in astrocytes via endogenous CB1 receptor agonists. Astrocytes in turn release glutamate that activates different metabotropic glutamate receptors of neurons and modulate PPN neuronal activity.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Endocannabinoid
Pedunculopontine nucleus
CB1 receptor
Neuromodulation
Astrocyte
Metabotropic glutamate receptor
Megjelenés:Brain structure and function 220 : 5 (2015), p. 3023-3041. -
További szerzők:Kovács Adrienn (1989-) (molekuláris biológus) Bíró Tamás (1968-) (élettanász) Szűcs Péter (1974-) (kutatóorvos) Vincze János (1988-) (orvos) Hegyi Zoltán (1983-) (molekuláris biológus) Antal Miklós (1951-) (orvos, anatómus) Pál Balázs (1975-) (élettanász)
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2.

001-es BibID:BIBFORM062728
Első szerző:Kovács Adrienn (molekuláris biológus)
Cím:Direct presynaptic and indirect astrocyte-mediated mechanisms both contribute to endocannabinoid signaling in the pedunculopontine nucleus of mice / Kovács A., Bordás Cs., Bíró T., Hegyi Z., Antal M., Szücs P., Pál B.
Dátum:2017
ISSN:1863-2653 1863-2661
Megjegyzések:The pedunculopontine nucleus (PPN), a cholinergic nucleus of the reticular activating system, is known to be involved in the regulation of sleep and wakefulness. Endogenous and exogenous cannabinoids, either by systemic or local administration to the pedunculopontine nucleus can both influence sleep. We previously demonstrated that activation of astrocytes by cannabinoid type 1 (CB1) receptor agonists was able to modulate the membrane potential of PPN neurons, even in the presence of blockers of fast synaptic neurotransmission. In the present work we provide evidence that synaptic inputs of PPN neurons are also affected by activation of presynaptic and astrocytic CB1 receptors.Using slice electrophysiology combined with calcium imaging, optogenetics and immunohistochemistry, we revealed a direct presynaptic inhibitory action on inhibitory postsynaptic currents, along with a mild increase of excitatory postsynaptic currents during CB1 receptor stimulation. Besides inhibition of excitatory and inhibitory neurotransmission through stimulation of presynaptic CB1 receptors, astrocyte- and mGluR-dependent tonic inhibition and excitation also developed. The mild stimulatory action of CB1 receptor activation on excitatory neurotransmission is the combination of astrocyte-dependent tonic excitation on excitatory neurons and the canonical presynaptic CB1 receptor activation and consequential inhibition of excitatory synaptic neurotransmission, whereas the astrocyte-dependent stimulatory action was not observed on inhibitory neurotransmission within the PPN.Our findings demonstrate that endocannabinoids act in the PPN via a dual pathway, consisting of a direct presynaptic and an indirect, astrocyte-mediated component, regulating synaptic strength and neuronal activity via independent mechanisms.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
pedunculopontine nucleus
CB1 receptor
optogenetics
astrocyte
neuromodulation
Megjelenés:Brain Structure & Function 222 : 1 (2017), p. 247-266. -
További szerzők:Bordás Csilla Bíró Tamás (1968-) (élettanász) Hegyi Zoltán (1983-) (molekuláris biológus) Antal Miklós (1951-) (orvos, anatómus) Szűcs Péter (1974-) (kutatóorvos) Pál Balázs (1975-) (élettanász)
Pályázati támogatás:Nemzeti Agykutatási Program KTIA_13_NAP-A-I/10
Egyéb
Nemzeti Agykutatási Program KTIA_NAP_13-1-2013-0001
Egyéb
Nemzeti Agykutatási Program KTIA_NAP_13-2-2014-0005
Egyéb
MTA-TKI 242
MTA
TÁMOP-4.2.2.B-15/1/KONV-2015-0001
TÁMOP
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
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3.

001-es BibID:BIBFORM085361
035-os BibID:(WoS)000537309400001 (Scopus)85085880838
Első szerző:Oláh Attila (élettanász)
Cím:Mitochondrial energy metabolism is negatively regulated by cannabinoid receptor 1 in intact human epidermis / Oláh Attila, Alam Majid, Chéret Jérémy, Kis Nikoletta Gréta, Hegyi Zoltán, Szöllősi Attila Gábor, Vidali Silvia, Bíró Tamás, Paus Ralf
Dátum:2020
ISSN:0906-6705
Megjegyzések:Epidermal energy metabolism is relevant to skin physiology, aging, and photodamage. While selected hormones stimulate epidermal keratinocyte mitochondrial activity, its negative regulation remains unknown. In several cell types, cannabinoid receptor 1 (CB1) is expressed both in the cell membrane (cmCB1), and in the mitochondrial outer membrane (mtCB1), where its stimulation directly suppresses mitochondrial functions. In the current pilot study, we investigated if CB1 is a negative regulator of human epidermal energy metabolism under physiological conditions. Using organ-cultured full-thickness human skin specimens of healthy individuals, we showed that antagonizing the homeostatic CB1 signaling by the administration of the CB1 inverse agonist AM251 increased activity of respiratory chain complex I and II/IV activity in a CB1-dependent manner, since the CB1-selective agonist arachidonyl-2'-chloroethylamide could prevent the effect. Moreover, the phenomenon was also reproduced by siRNA-mediated down-regulation of CB1. As revealed by the unaltered expression of several relevant markers (TFAM, VDAC1, MTCO1, and NDUFS4), modulation of CB1 signaling had no effect on the epidermal mitochondrial mass. Next, by using immunoelectron microscopy, we found that human epidermal keratinocytes express both cmCB1 and mtCB1. Finally, by using equipotent extracellularly-restricted (hemopressin) as well as cell-permeable (AM251) inverse agonists, we found that mitochondrial activity is most likely exclusively regulated by mtCB1. Thus, our data identify mtCB1 as a negative regulator of keratinocyte mitochondrial activity in intact human epidermis, and highlight the question, whether topical therapeutic interventions capable of selectively activating mtCB1 can reduce excessive mitochondrial ROS production resulting from dysregulated mitochondrial activity during skin aging or photodamage.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
endocannabinoid
epidermal keratinocyte
mitochondrial activity
mitochondrially expressed CB1 (mtCB1)
cannabinoid receptor 1 (CB1)
Megjelenés:Experimental Dermatology. - 29 : 7 (2020), p. 616-622. -
További szerzők:Alam, Majid Chéret, Jérémy Kis Gréta (1979-) (környezetkutató) Hegyi Zoltán (1983-) (molekuláris biológus) Szöllősi Attila Gábor (1982-) (élettanász) Vidali, Silvia Bíró Tamás (1968-) (élettanász) Paus, Ralf
Pályázati támogatás:121360
OTKA
125055
OTKA
Hungarian National Brain Research Program [KTIA_NAP_13-1-2013-001]
Egyéb
Bolyai János Kutatási Ösztöndíj
MTA
ITM - Új Nemzeti Kiválóság Program ÚNKP-19-4-DE-287
Egyéb
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Intézményi repozitóriumban (DEA) tárolt változat
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