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001-es BibID:BIBFORM016014
Első szerző:Bodó Enikő
Cím:Thyroid-Stimulating Hormone, a Novel, Locally Produced Modulator of Human Epidermal Functions, Is Regulated by Thyrotropin-Releasing Hormone and Thyroid Hormones / Bodo, E., Kany, B., Gaspar, E., Knuver, J., Kromminga, A., Ramot, Y., Biro, T., Tiede, S., van Beek, N., Poeggeler, B., Meyer, K. C., Wenzel, B. E., Paus, R.
Dátum:2010
ISSN:0013-7227
Megjegyzések:Several elements of the hypothalamic-pituitary-thyroid axis (HPT) reportedly are transcribed by human skin cell populations, and human hair follicles express functional receptors for TSH. Therefore, we asked whether the epidermis of normal human skin is yet another extrathyroidal target of TSH and whether epidermis even produces TSH. If so, we wanted to clarify whether intraepidermal TSH expression is regulated by TRH and/or thyroid hormones and whether TSH alters selected functions of normal human epidermis in situ. TSH and TSH receptor (TSH-R) expression were analyzed in the epidermis of normal human scalp skin by immunohistochemistry and PCR. In addition, full-thickness scalp skin was organ cultured and treated with TSH, TRH, or thyroid hormones, and the effect of TSH treatment on the expression of selected genes was measured by quantitative PCR and/or quantitative immunohistochemistry. Here we show that normal human epidermis expresses TSH at the mRNA and protein levels in situ and transcribes TSH-R. It also contains thyrostimulin transcripts. Intraepidermal TSH immunoreactivity is up-regulated by TRH and down-regulated by thyroid hormones. Although TSH-R immunoreactivity in situ could not be documented within the epidermis, but in the immediately adjacent dermis, TSH treatment of organ-cultured human skin strongly up-regulated epidermal expression of involucrin, loricrin, and keratins 5 and 14. Thus, normal human epidermis in situ is both an extrapituitary source and (possibly an indirect) target of TSH signaling, which regulates defined epidermal parameters. Intraepidermal TSH expression appears to be regulated by the classical endocrine controls that determine the systemic HPT axis.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Endocrinology. - 151 : 4 (2010), p. 1633-1642. -
További szerzők:Kany Benedikt Gáspár Erzsébet Knüver, Jana Kromminga, Arno Ramot, Yuval Bíró Tamás (1968-) (élettanász) Tiede, Stephan van Beek, Nina Poeggeler, Burkhard Meyer, Katja C. Wenzel, Björn E. Paus, Ralf
Internet cím:DOI
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2.

001-es BibID:BIBFORM009197
Első szerző:Bodó Enikő
Cím:Human female hair follicles are a direct, nonclassical target for thyroid-stimulating hormone / Bodo, E., Kromminga, A., Biro, T., Borbiro, I., Gaspar, E., Zmijewski, M. A., van Beek, N., Langbein, L., Slominski, A. T., Paus, R.
Dátum:2009
ISSN:1523-1747 (Electronic)
Megjegyzések:Pituitary thyroid-stimulating hormone (TSH) regulates thyroid hormone synthesis via receptors (TSH-R) expressed on thyroid epithelial cells. As the hair follicle (HF) is uniquely hormone-sensitive and, hypothyroidism with its associated, increased TSH serum levels clinically can lead to hair loss, we asked whether human HFs are a direct target for TSH. Here, we report that normal human scalp skin and microdissected human HFs express TSH-R mRNA. TSH-R-like immunoreactivity is limited to the mesenchymal skin compartments in situ. TSH may alter HF mesenchymal functions, as it upregulates alpha-smooth muscle actin expression in HF fibroblasts. TSH-R stimulation by its natural ligand in organ culture changes the expression of several genes of human scalp HFs (for example keratin K5), upregulates the transcription of classical TSH target genes and enhances cAMP production. Although the functional role of TSH in human HF biology awaits further dissection, these findings document that intracutaneous TSH-Rs are fully functional in situ and that HFs of female individuals are direct targets for nonclassical, extrathyroidal TSH bioregulation. This suggests that organ-cultured scalp HFs provide an instructive and physiologically relevant human model for exploring nonclassical functions of TSH, in and beyond the skin.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Actins
Cell Proliferation
Cells, Cultured
Cyclic AMP
Female
Fibroblasts
Hair Follicle
Humans
Keratin-5
Mesoderm
Nuclear Proteins
Organ Culture Techniques
RNA, Messenger
Receptors, Thyrotropin
Scalp
Skin
Thyroglobulin
Thyrotropin
Transcription Factors
Megjelenés:The Journal of Investigative Dermatology. - 129 : 5 (2009), p. 1126-1139. -
További szerzők:Kromminga, Arno Bíró Tamás (1968-) (élettanász) Borbíró István (1982-) (biokémikus, molekuláris biológus) Gáspár Erzsébet Zmijewski, Michal A. van Beek, Nina Langbein, Lutz Slominski, Andrzej T. Paus, Ralf
Internet cím:DOI
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3.

001-es BibID:BIBFORM039252
Első szerző:Gáspár Erzsébet
Cím:Thyrotropin-Releasing Hormone Selectively Stimulates Human Hair Follicle Pigmentation / Gáspár, Erzsébet, Nguyen-Thi, Kim T, Hardenbicker Celine, Tiede Stephan, Plate Christian, Bodó Enikő, Knuever Jana, Funk Wolfgang, Bíró Tamás, Paus Ralf
Dátum:2011
ISSN:0022-202X
Megjegyzések:In amphibians, thyrotropin-releasing hormone (TRH) stimulates skin melanophores by inducing secretion of α-melanocyte-stimulating hormone in the pituitary gland. However, it is unknown whether this tripeptide neurohormone exerts any direct effects on pigment cells, namely, on human melanocytes, under physiological conditions. Therefore, we have investigated whether TRH stimulates pigment production in organ-cultured human hair follicles (HFs), the epithelium of which expresses both TRH and its receptor, and/or in full-thickness human skin in situ. TRH stimulated melanin synthesis, tyrosinase transcription and activity, melanosome formation, melanocyte dendricity, gp100 immunoreactivity, and microphthalmia-associated transcription factor expression in human HFs in a pituitary gland-independent manner. TRH also stimulated proliferation, gp100 expression, tyrosinase activity, and dendricity of isolated human HF melanocytes. However, intraepidermal melanogenesis was unaffected. As TRH upregulated the intrafollicular production of "pituitary" neurohormones (proopiomelanocortin transcription and ACTH immunoreactivity) and as agouti-signaling protein counteracted TRH-induced HF pigmentation, these pigmentary TRH effects may be mediated in part by locally generated melanocortins and/or by MC-1 signaling. Our study introduces TRH as a novel, potent, selective, and evolutionarily highly conserved neuroendocrine factor controlling human pigmentation in situ. This physiologically relevant and melanocyte sub-population-specific neuroendocrine control of human pigmentation deserves clinical exploration, e.g., for preventing or reversing hair graying.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Journal Of Investigative Dermatology. - 131 : 12 (2011), p. 2368-2377. -
További szerzők:Nguyen-Thi, Kim T Hardenbicker, Celine Tiede, Stephan Plate, Christian Bodó Enikő Knüver, Jana Funk, Wolfgang Bíró Tamás (1968-) (élettanász) Paus, Ralf
Internet cím:Szerző által megadott URL
DOI
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4.

001-es BibID:BIBFORM016018
Első szerző:Poeggeler, Burkhard
Cím:Thyrotropin powers human mitochondria / Poeggeler, B., Knuever, J., Gaspar, E., Biro, T., Klinger, M., Bodo, E., Wiesner, R. J., Wenzel, B. E., Paus, R.
Dátum:2010
ISSN:0892-6638
Megjegyzések:Here we demonstrate that the neuropeptide hormone thyrotropin (TSH), which controls thyroid hormone production, exerts a major nonclassical function in mitochondrial biology. Based on transcriptional, ultrastructural, immunohistochemical, and biochemical evidence, TSH up-regulates mitochondrial biogenesis and consequently activity in organ-cultured normal human epidermis in situ. Mitochondrial activity was assessed by measuring 2 key components of the respiratory chain. The abundance of mitochondria was assessed employing 2 independent morphological techniques: counting their numbers in human epidermis by high-magnification light microscopy of skin sections immunostained for mitochondria-selective cytochromec-oxidase subunit 1 (MTCO1) and transmission electron microscopy (TEM). Treatment with 10 mU/ml of TSH for 6 d strongly up-regulates the number of light-microscopically visualized, MTCO1-demarcated mitochondria. On the ultrastructural level, TEM confirms that TSH indeed stimulates mitochondrial proliferation and biogenesis in the perinuclear region of human skin epidermal keratinocytes. On the transcriptional level, TSH up-regulates MTCO1 mRNA (quantitative RT-PCR) and significantly enhances complex I and IV (cytochrome-c-oxidase) activity. This study pioneers the concept that mitochondrial energy metabolism and biogenesis in a normal, prototypic human epithelial tissue underlies potent neuroendocrine controls and introduces human skin organ culture as a clinically relevant tool for further exploring this novel research frontier in the control of mitochondrial biology.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:The FASEB Journal. - 24 : 5 (2010), p. 1525-1531. -
További szerzők:Knüver, Jana Gáspár Erzsébet Bíró Tamás (1968-) (élettanász) Klinger, Matthias Bodó Enikő Wiesner, Rudolf J. Wenzel, Björn E. Paus, Ralf
Internet cím:DOI
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