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001-es BibID:	BIBFORM016014
Első szerző:	Bodó Enikő
Cím:	Thyroid-Stimulating Hormone, a Novel, Locally Produced Modulator of Human Epidermal Functions, Is Regulated by Thyrotropin-Releasing Hormone and Thyroid Hormones / Bodo, E., Kany, B., Gaspar, E., Knuver, J., Kromminga, A., Ramot, Y., Biro, T., Tiede, S., van Beek, N., Poeggeler, B., Meyer, K. C., Wenzel, B. E., Paus, R.
Dátum:	2010
ISSN:	0013-7227
Megjegyzések:	Several elements of the hypothalamic-pituitary-thyroid axis (HPT) reportedly are transcribed by human skin cell populations, and human hair follicles express functional receptors for TSH. Therefore, we asked whether the epidermis of normal human skin is yet another extrathyroidal target of TSH and whether epidermis even produces TSH. If so, we wanted to clarify whether intraepidermal TSH expression is regulated by TRH and/or thyroid hormones and whether TSH alters selected functions of normal human epidermis in situ. TSH and TSH receptor (TSH-R) expression were analyzed in the epidermis of normal human scalp skin by immunohistochemistry and PCR. In addition, full-thickness scalp skin was organ cultured and treated with TSH, TRH, or thyroid hormones, and the effect of TSH treatment on the expression of selected genes was measured by quantitative PCR and/or quantitative immunohistochemistry. Here we show that normal human epidermis expresses TSH at the mRNA and protein levels in situ and transcribes TSH-R. It also contains thyrostimulin transcripts. Intraepidermal TSH immunoreactivity is up-regulated by TRH and down-regulated by thyroid hormones. Although TSH-R immunoreactivity in situ could not be documented within the epidermis, but in the immediately adjacent dermis, TSH treatment of organ-cultured human skin strongly up-regulated epidermal expression of involucrin, loricrin, and keratins 5 and 14. Thus, normal human epidermis in situ is both an extrapituitary source and (possibly an indirect) target of TSH signaling, which regulates defined epidermal parameters. Intraepidermal TSH expression appears to be regulated by the classical endocrine controls that determine the systemic HPT axis.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	Endocrinology. - 151 : 4 (2010), p. 1633-1642. -
További szerzők:	Kany Benedikt Gáspár Erzsébet Knüver, Jana Kromminga, Arno Ramot, Yuval Bíró Tamás (1968-) (élettanász) Tiede, Stephan van Beek, Nina Poeggeler, Burkhard Meyer, Katja C. Wenzel, Björn E. Paus, Ralf
Internet cím:	DOI Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM016018
Első szerző:	Poeggeler, Burkhard
Cím:	Thyrotropin powers human mitochondria / Poeggeler, B., Knuever, J., Gaspar, E., Biro, T., Klinger, M., Bodo, E., Wiesner, R. J., Wenzel, B. E., Paus, R.
Dátum:	2010
ISSN:	0892-6638
Megjegyzések:	Here we demonstrate that the neuropeptide hormone thyrotropin (TSH), which controls thyroid hormone production, exerts a major nonclassical function in mitochondrial biology. Based on transcriptional, ultrastructural, immunohistochemical, and biochemical evidence, TSH up-regulates mitochondrial biogenesis and consequently activity in organ-cultured normal human epidermis in situ. Mitochondrial activity was assessed by measuring 2 key components of the respiratory chain. The abundance of mitochondria was assessed employing 2 independent morphological techniques: counting their numbers in human epidermis by high-magnification light microscopy of skin sections immunostained for mitochondria-selective cytochromec-oxidase subunit 1 (MTCO1) and transmission electron microscopy (TEM). Treatment with 10 mU/ml of TSH for 6 d strongly up-regulates the number of light-microscopically visualized, MTCO1-demarcated mitochondria. On the ultrastructural level, TEM confirms that TSH indeed stimulates mitochondrial proliferation and biogenesis in the perinuclear region of human skin epidermal keratinocytes. On the transcriptional level, TSH up-regulates MTCO1 mRNA (quantitative RT-PCR) and significantly enhances complex I and IV (cytochrome-c-oxidase) activity. This study pioneers the concept that mitochondrial energy metabolism and biogenesis in a normal, prototypic human epithelial tissue underlies potent neuroendocrine controls and introduces human skin organ culture as a clinically relevant tool for further exploring this novel research frontier in the control of mitochondrial biology.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	The FASEB Journal. - 24 : 5 (2010), p. 1525-1531. -
További szerzők:	Knüver, Jana Gáspár Erzsébet Bíró Tamás (1968-) (élettanász) Klinger, Matthias Bodó Enikő Wiesner, Rudolf J. Wenzel, Björn E. Paus, Ralf
Internet cím:	DOI Intézményi repozitóriumban (DEA) tárolt változat
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